過氧化物酶體增殖物激活受體γ(PPARg)檢測試劑盒DIY材料(酶聯(lián)免疫吸附試驗(yàn)法)
ELISA Kit DIY Materials for Peroxisome Proliferator Activated Receptor Gamma (PPARg)
PPAR-G; PPARG1; PPARG2; NR1C3; Glitazone Receptor; Nuclear Receptor Subfamily 1 Group C Member 3
- 編號(hào)KSA886Ra01
- 物種Rattus norvegicus (Rat，大鼠) 相同的名稱，不同的物種。
- 試劑內(nèi)容 包被抗體、檢測抗體、標(biāo)準(zhǔn)品、HRP標(biāo)記的鏈霉親和素、TMB底物、96孔板
- 可檢測樣本Serum, plasma, tissue homogenates, cell lysates, cell culture supernates and other biological fluids。
- 可適用原理雙抗夾心酶聯(lián)免疫吸附試驗(yàn)法測抗原
- 可檢測范圍0.156-10ng/mL
- 可適靈敏度0.056ng/mL
- 應(yīng)用Main materials for "Do It (ELISA Kit) Yourself".
- 下載 英文說明書 中文說明書
- 規(guī)格 96T*596T*10 96T*20 96T*50 96T*100
- 價(jià)格 ¥ 7387 ¥ 12312 ¥ 22162 ¥ 43092 ¥ 73872
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產(chǎn)品包裝(模擬)
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通過ISO 9001、ISO 13485質(zhì)量體系認(rèn)證
特異性
試劑盒中的抗體對(duì)過氧化物酶體增殖物激活受體γ(PPARg)具有高靈敏度和高特異性，經(jīng)檢測與其它相似物質(zhì)無明顯交叉反應(yīng)。
用法
1.用100μL每孔的工作濃度的包被抗體包被96孔板，4度孵育過夜或者37度孵育2小時(shí)；
2.洗板1次；
3.用200μL每孔的工作濃度的封閉液,37度孵育1.5小時(shí)；
4.洗板1次。酶標(biāo)板即可用于ELISA實(shí)驗(yàn)，后續(xù)使用方法與ELISA同。
儲(chǔ)存
抗體，標(biāo)準(zhǔn)品和HRP標(biāo)記的鏈霉親和素在-20度保存，TMB需在4度保存，96孔板在室溫保存。所有組分保質(zhì)期為1年，試劑一旦開封，在4度保存時(shí)間為1個(gè)月。
配套試劑
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相關(guān)產(chǎn)品
| 編號(hào) | 適用物種：Rattus norvegicus (Rat，大鼠) | 應(yīng)用(僅供研究使用，不用于臨床診斷！) |
| RPA886Ra01 | 過氧化物酶體增殖物激活受體γ(PPARg)重組蛋白 | Positive Control; Immunogen; SDS-PAGE; WB. |
| PAA886Ra01 | 過氧化物酶體增殖物激活受體γ(PPARg)多克隆抗體 | WB; IHC; ICC; IP. |
| MAA886Ra22 | 過氧化物酶體增殖物激活受體γ(PPARg)單克隆抗體 | WB; IHC; ICC; IP. |
| MAA886Ra23 | 過氧化物酶體增殖物激活受體γ(PPARg)單克隆抗體 | WB; IHC; ICC; IP. |
| MAA886Ra24 | 過氧化物酶體增殖物激活受體γ(PPARg)單克隆抗體 | WB; IHC; ICC; IP. |
| MAA886Ra25 | 過氧化物酶體增殖物激活受體γ(PPARg)單克隆抗體 | WB; IHC; ICC; IP. |
| MAA886Ra21 | 過氧化物酶體增殖物激活受體γ(PPARg)單克隆抗體 | WB,IHC |
| SEA886Ra | 過氧化物酶體增殖物激活受體γ(PPARg)檢測試劑盒(酶聯(lián)免疫吸附試驗(yàn)法) | Enzyme-linked immunosorbent assay for Antigen Detection. |
| LMA886Ra | 過氧化物酶體增殖物激活受體γ(PPARg)等多因子檢測試劑盒(流式熒光發(fā)光法) | FLIA Kit for Antigen Detection. |
| KSA886Ra01 | 過氧化物酶體增殖物激活受體γ(PPARg)檢測試劑盒DIY材料(酶聯(lián)免疫吸附試驗(yàn)法) | Main materials for "Do It (ELISA Kit) Yourself". |
參考文獻(xiàn)
| 雜志 | 參考文獻(xiàn) |
| Neuroscience Letters | Elevated levels of PPAR-gamma in the cerebrospinal fluid of patients with multiple sclerosis [Pubmed: 24021801] |
| J Cell Biochem. | Nonivamide enhances miRNA let‐7d expression and decreases adipogenesis PPARγ expression in 3T3‐L1 cells [Pubmed:25704235] |
| Osteoarthritis Cartilage | Establishment of a rabbit model to study the influence of advanced glycation end products accumulation on osteoarthritis and the protective effect of pioglitazone [PubMed: 26321377] |
| J Clin Diagn Res. | Evaluation of Protein Kinase Cβ and PPARγ Activity in Diabetic Rats Supplemented with Momordica charantia [pmc:PMC4866090] |
| Journal of Clinical&Diagnostic Research | Evaluation?of?Protein?Kinase?Cβ and PPARγ?Activity?in?Diabetic?RatsSupplemented?with?Momordica?charantia. [pubmed:27190792] |
| Osteoarthritis?and?Cartilage | Establishment?of a?rabbit?model?to?study?the?influence?of?advanced?glycation?end?productsaccumulation?on?osteoarthritis?and the?protective?effect?of pioglitazone. [pubmed:26321377] |
| Neuroscience Letters | Unlike PPARgamma, neither other PPARs nor PGC-1alpha is elevated in the cerebrospinal fluid of patients with multiple sclerosis [pubmed:28483651] |
| European?Journal?of?Immunology | Engulfment of Hb‐activated platelets differentiates monocytes into pro‐inflammatory macrophages in PNH patients [Pubmed:29677388] |
| Histochemistry and?Cell Biology | Simpson–Golabi–Behmel syndrome human adipocytes reveal a changing phenotype throughout differentiation [Pubmed:29574488] |
| molecular and cellular biochemistry | Maternal omega-3 fatty acids and vitamin E improve placental angiogenesis in late-onset but not early-onset preeclampsia [Pubmed: 31420792] |
| Nutrients.? | Hyperglycemia Changes Expression of Key Adipogenesis Markers (C/EBPα and PPAR?) and Morphology of Differentiating Human Visceral Adipocytes [Pubmed: 31398873] |
| journal of biochemical and molecular toxicology | Indomethacin and juglone inhibit inflammatory molecules to induce apoptosis in colon cancer cells [Pubmed: 31916655] |
| J Cosmet Dermatol | In?\vitro effect of pine bark extract on melanin synthesis, tyrosinase activity, production of endothelin?\1 and PPAR in cultured melanocytes exposed to Ultraviolet??- [33960120] |
| American Chemical Society | TRPA1 Agonist Cinnamaldehyde Decreases Adipogenesis in 3T3-L1 Cells More Potently than the Non-agonist Structural Analog Cinnamyl Isobutyrate [33403292] |
| PLoS One | Quantitative real-time measurement of endothelin-1-induced contraction in single non-activated hepatic stellate cells [34343209] |
| Prostaglandins Leukot Essent Fatty Acids | Maternal Vitamin D Deficiency Reduces Docosahexaenoic Acid, Placental Growth Factor and Peroxisome Proliferator Activated Receptor Gamma levels in the Pup?… [34768025] |
| Food and Chemical Toxicology | Melatonin attenuates cisplatin-induced acute kidney injury in mice: Involvement of PPARα and fatty acid oxidation [Pubmed:35367536] |
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