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色素上皮衍生因子(PEDF)檢測試劑盒DIY材料(酶聯(lián)免疫吸附試驗法)

ELISA Kit DIY Materials for Pigment Epithelium Derived Factor (PEDF)

SERPINF1; EPC1; PIG35; SDF3; Serpin F1; Cell proliferation-inducing gene 35; Serpin Peptidase Inhibitor,Clade F Member 1(Alpha-2 Antiplasmin); Stromal Cell Derived Factor 3

  • 色素上皮衍生因子(PEDF)檢測試劑盒DIY材料(酶聯(lián)免疫吸附試驗法) 產(chǎn)品包裝(模擬)
  • 色素上皮衍生因子(PEDF)檢測試劑盒DIY材料(酶聯(lián)免疫吸附試驗法) 產(chǎn)品包裝(模擬)
  • Certificate 通過ISO 9001、ISO 13485質(zhì)量體系認證

特異性

試劑盒中的抗體對色素上皮衍生因子(PEDF)具有高靈敏度和高特異性，經(jīng)檢測與其它相似物質(zhì)無明顯交叉反應。

用法

1.用100μL每孔的工作濃度的包被抗體包被96孔板，4度孵育過夜或者37度孵育2小時；
2.洗板1次；
3.用200μL每孔的工作濃度的封閉液,37度孵育1.5小時；
4.洗板1次。酶標板即可用于ELISA實驗，后續(xù)使用方法與ELISA同。

儲存

抗體，標準品和HRP標記的鏈霉親和素在-20度保存，TMB需在4度保存，96孔板在室溫保存。所有組分保質(zhì)期為1年，試劑一旦開封，在4度保存時間為1個月。

配套試劑

相關產(chǎn)品

編號 適用物種：Mus musculus (Mouse，小鼠) 應用(僅供研究使用，不用于臨床診斷！)
RPB972Mu01 色素上皮衍生因子(PEDF)重組蛋白 Positive Control; Immunogen; SDS-PAGE; WB.
RPB972Mu02 色素上皮衍生因子(PEDF)重組蛋白 Positive Control; Immunogen; SDS-PAGE; WB.
RPB972Mu03 色素上皮衍生因子(PEDF)重組蛋白 Positive Control; Immunogen; SDS-PAGE; WB.
PAB972Mu01 色素上皮衍生因子(PEDF)多克隆抗體 WB; IHC; ICC; IP.
PAB972Mu03 色素上皮衍生因子(PEDF)多克隆抗體 WB; IHC; ICC; IP.
PAB972Mu02 色素上皮衍生因子(PEDF)多克隆抗體 WB; IHC; ICC; IP.
LAB972Mu81 色素上皮衍生因子(PEDF)多克隆抗體(異硫氰酸熒光素標記) WB; IHC; ICC; IF.
LAB972Mu71 色素上皮衍生因子(PEDF)多克隆抗體(生物素標記) WB; IHC; ICC.
MAB972Mu21 色素上皮衍生因子(PEDF)單克隆抗體 WB,IHC
MAB972Mu22 色素上皮衍生因子(PEDF)單克隆抗體 WB; IHC; ICC; IP.
MEB972Mu 色素上皮衍生因子(PEDF)檢測試劑盒(酶聯(lián)免疫吸附試驗法,小樣本) Enzyme-linked immunosorbent assay for Antigen Detection.
SEB972Mu 色素上皮衍生因子(PEDF)檢測試劑盒(酶聯(lián)免疫吸附試驗法) Enzyme-linked immunosorbent assay for Antigen Detection.
SCB972Mu 色素上皮衍生因子(PEDF)檢測試劑盒(化學發(fā)光免疫分析法) Chemiluminescent immunoassay for Antigen Detection.
LMB972Mu 色素上皮衍生因子(PEDF)等多因子檢測試劑盒(流式熒光發(fā)光法) FLIA Kit for Antigen Detection.
KSB972Mu01 色素上皮衍生因子(PEDF)檢測試劑盒DIY材料(酶聯(lián)免疫吸附試驗法) Main materials for "Do It (ELISA Kit) Yourself".

參考文獻

雜志 參考文獻
Molecular Therapy A Therapeutic Strategy for Choroidal Neovascularization Based on Recruitment of Mesenchymal Stem Cells to the Sites of Lesions [Nature: 2010144]
American Journal of Nephrology Urinary Pigment Epithelium-Derived Factor as a Marker of Diabetic Nephropathy [Karger: 314326]
34 Increased expression of pigment epithelium-derived factor in aged mesenchymal stem cells impairs their therapeutic efficacy for attenuating myocardial infarction injury [OxfordJournals: source]
Heart and Education in Heart The change of serum level of pigment epithelium-derived factor in coronary heart disease [Bmj: Source]
Mediators of Inflammation Metformin Inhibits Expression and Secretion of PEDF in Adipocyte and Hepatocyte via Promoting AMPK Phosphorylation [Pubmed: 24288442]
Cardiology. The association study of plasma levels of pigment epithelium-derived factor with acute coronary syndrome in the chinese han population. [Pubmed: 24192856]
Endocrinology Rosiglitazone Inhibits Expression and Secretion of PEDF in Adipose Tissue and Liver of Male SD Rats Via a PPAR-γ Independent Mechanism [Endocrine: Source]
Clinical Science Pigment epithelium-derived factor regulates microvascular permeability through adipose triglyceride lipase in sepsis [PubMed: 25700221]
Molecular Sciences Pigment Epithelium-Derived Factor (PEDF) Protects Osteoblastic Cell Line from Glucocorticoid-Induced Apoptosis via PEDF-R [Pubmed:27187377]
Investigative ophthalmoloey & visual science The Ingenious Interactions Between Macrophages and Functionally Plastic Retinal Pigment Epithelium Cells [articleid:2582822]
Scientific reports VEGF-B promotes recovery of corneal innervations and trophic functions in diabetic mice [pubmed:28091556]
國際眼科雜志 色素上皮衍生因子修飾的人臍帶間充質(zhì)干細胞構建方法 [201707007]
Investigative?Ophthalmology?&?Visual?Science PEDF Reduces the Severity of Herpetic Simplex Keratitis in Mice [Pubmed:30025136]
International Eye Science 高海拔地區(qū)新生血管性青光眼不同治療方式的療效及對房水中PEDF, VEGF 水平的影響 [2018/2/201802020.pdf]
Domestic?Animal?Endocrinology Lactation-related changes in tissue expression of PEDF in dairy cows [Pubmed:29758402]
Medical?Science?Monitor Correlation Between Pigment Epithelium-Derived Factor (PEDF) level and Degree of Coronary Angiography and Severity of Coronary Artery Disease in a?… [Pubmed:29574467]
Chemosphere Role of pigment epithelium-derived factor (PEDF) on arsenic-induced neuronal apoptosis [Doi: 10.1016/j.chemosphere.2018.10.100]
Biological?Trace?Element?Research Role of Pigment Epithelium-Derived Factor in Arsenic-Induced Vascular Endothelial Dysfunction in a Rat Model [Pubmed: 30392020]
International?Urology?and Nephrology Increased levels of serum pigment epithelium-derived factor aggravate proteinuria via induction of podocyte actin rearrangement [Pubmed: 30536192]
International Journal of Ophthalmology A novel xeno-free culture system for human retinal pigment epithelium cells [Pubmed: 31024807]
Journal of the American Heart Association Pigment Epithelial‐Derived Factor Deficiency Accelerates Atherosclerosis Development via Promoting Endothelial Fatty Acid Uptake in Mice With Hyperlipidemia [Pubmed: 31711388]
environmental research Pigment epithelium-derived factor (PEDF) ameliorates arsenic-induced vascular endothelial dysfunction in rats and toxicity in endothelial EA. hy926 cells [Pubmed: 32315827]
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES The Expression Levels and Cellular Localization of Pigment Epithelium Derived Factor (PEDF) in Mouse Testis: Its Possible Involvement in the Differentiation of Spermatogonial Cells [33498962]
Hum Cell Long non-coding RNA Rian promotes the expression of tight junction proteins in endothelial cells by regulating perivascular-resident macrophage-like melanocytes ?- [33768511]
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