表面活性物質(zhì)關(guān)聯(lián)蛋白D(SPD)檢測(cè)試劑盒DIY材料(酶聯(lián)免疫吸附試驗(yàn)法)
ELISA Kit DIY Materials for Surfactant Associated Protein D (SPD)
SFTPD; COLEC7; PSP-D; SFTP4; SP-D; Pulmonary Surfactant Protein D; Collectin-7; Lung surfactant protein D
- 編號(hào)KSB039Hu01
- 物種Homo sapiens (Human，人) 相同的名稱，不同的物種。
- 試劑內(nèi)容 包被抗體、檢測(cè)抗體、標(biāo)準(zhǔn)品、HRP標(biāo)記的鏈霉親和素、TMB底物、96孔板
- 可檢測(cè)樣本serum, plasma, tissue homogenates, cell lysates, lung lavage fluid, cell culture supernates and other biological fluids.
- 可適用原理雙抗夾心酶聯(lián)免疫吸附試驗(yàn)法測(cè)抗原
- 可檢測(cè)范圍0.625-40ng/mL
- 可適靈敏度0.233ng/mL
- 應(yīng)用Main materials for "Do It (ELISA Kit) Yourself".
- 下載 英文說(shuō)明書(shū) 中文說(shuō)明書(shū)
- 規(guī)格 96T*596T*10 96T*20 96T*50 96T*100
- 價(jià)格 ¥ 5378 ¥ 8964 ¥ 16135 ¥ 31374 ¥ 53784
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通過(guò)ISO 9001、ISO 13485質(zhì)量體系認(rèn)證
特異性
試劑盒中的抗體對(duì)表面活性物質(zhì)關(guān)聯(lián)蛋白D(SPD)具有高靈敏度和高特異性，經(jīng)檢測(cè)與其它相似物質(zhì)無(wú)明顯交叉反應(yīng)。
用法
1.用100μL每孔的工作濃度的包被抗體包被96孔板，4度孵育過(guò)夜或者37度孵育2小時(shí)；
2.洗板1次；
3.用200μL每孔的工作濃度的封閉液,37度孵育1.5小時(shí)；
4.洗板1次。酶標(biāo)板即可用于ELISA實(shí)驗(yàn)，后續(xù)使用方法與ELISA同。
儲(chǔ)存
抗體，標(biāo)準(zhǔn)品和HRP標(biāo)記的鏈霉親和素在-20度保存，TMB需在4度保存，96孔板在室溫保存。所有組分保質(zhì)期為1年，試劑一旦開(kāi)封，在4度保存時(shí)間為1個(gè)月。
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參考文獻(xiàn)
| 雜志 | 參考文獻(xiàn) |
| The American Journal of Rhinology & Allergy | Surfactant protein A and D in chronic rhinosinusitis with nasal polyposis and corticosteroid response [Ingenta: art00005] |
| J Occup Health | Evaluation of acute oxidative stress induced by NiO nanoparticles in vivo and in vitro [PubMed: 21233593] |
| Inhalation Toxicology | Comparison of acute oxidative stress on rat lung induced by nano and fine-scale, soluble and insoluble metal oxide particles: NiO and TiO2 [PubMed: 22642288] |
| American Journal of Rhinology & Allergy | Detection of surfactant proteins A, B, C, and D in human nasal mucosa and their regulation in chronic rhinosinusitis with polyps [PubMed: 23406594] |
| Plos one | Staphylococcus aureus and Pseudomonas aeruginosa Express and Secrete Human Surfactant Proteins [PubMed: PMC3551896] |
| Plos one | The Detection of Surfactant Proteins A, B, C and D in the Human Brain and Their Regulation in Cerebral Infarction, Autoimmune Conditions and Infections of the CNS [PubMed: PMC3787032] |
| Nachweis und Charakterisierung des Oberfl?chenproteins PLUNC (Palate, Lung and Nasal Clone Protein) an der Augenoberfl?che und Bedeutung für das Trockene Auge [Opus4:Source] | |
| PLoS One | The?Cerebral?Surfactant?System?and Its?Alteration?in?HydrocephalicConditions. [pubmed:27656877] |
| Frontiers in Aging Neuroscience | Correlations?of?Ventricular?Enlargement?with?Rheologically?Active?SurfactantProteins?in?Cerebrospinal?Fluid. [pubmed:28101052] |
| American Journal of Physiology. Lung Cellular and Molecular Physiology | Chronic lung injury and impaired pulmonary function in a mouse model of acid ceramidase deficiency. [pubmed:29167126] |
| Molecular Neurobiology | Rheologically Essential Surfactant Proteins of the CSF Interacting with Periventricular White Matter Changes in Hydrocephalus Patients–Implications for CSF?… [Doi: 10.1007/s12035-019-01648-z] |
| Respir Res | Elevated plasma levels of epithelial and endothelial cell markers in COVID-19 survivors with reduced lung diffusing capacity six months after hospital?… [Pubmed:35189887] |
| Virology Journal | The association between sPD-1 levels versus liver biochemistry and viral markers in chronic hepatitis B patients: a comparative study of different sPD-1?… [Pubmed:35361235] |
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