無(wú)翅型MMTV整合位點(diǎn)家族成員3A(WNT3A)檢測(cè)試劑盒DIY材料(酶聯(lián)免疫吸附試驗(yàn)法)
ELISA Kit DIY Materials for Wingless Type MMTV Integration Site Family, Member 3A (WNT3A)
- 編號(hào)KSP155Mu01
- 物種Mus musculus (Mouse,小鼠) 相同的名稱,不同的物種。
- 試劑內(nèi)容 包被抗體、檢測(cè)抗體、標(biāo)準(zhǔn)品、HRP標(biāo)記的鏈霉親和素、TMB底物、96孔板
- 可檢測(cè)樣本Tissue homogenates, cell lysates, cell culture supernates and other biological fluids.
- 可適用原理雙抗夾心酶聯(lián)免疫吸附試驗(yàn)法測(cè)抗原
- 可檢測(cè)范圍0.156-10ng/mL
- 可適靈敏度0.054ng/mL
- 應(yīng)用Main materials for "Do It (ELISA Kit) Yourself".
- 下載 英文說(shuō)明書(shū) 中文說(shuō)明書(shū)
- 規(guī)格 96T*596T*10 96T*20 96T*50 96T*100
- 價(jià)格 ¥ 7776 ¥ 12960 ¥ 23328 ¥ 45360 ¥ 77760
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特異性
試劑盒中的抗體對(duì)無(wú)翅型MMTV整合位點(diǎn)家族成員3A(WNT3A)具有高靈敏度和高特異性,經(jīng)檢測(cè)與其它相似物質(zhì)無(wú)明顯交叉反應(yīng)。
用法
1.用100μL每孔的工作濃度的包被抗體包被96孔板,4度孵育過(guò)夜或者37度孵育2小時(shí);
2.洗板1次;
3.用200μL每孔的工作濃度的封閉液,37度孵育1.5小時(shí);
4.洗板1次。酶標(biāo)板即可用于ELISA實(shí)驗(yàn),后續(xù)使用方法與ELISA同。
儲(chǔ)存
抗體,標(biāo)準(zhǔn)品和HRP標(biāo)記的鏈霉親和素在-20度保存,TMB需在4度保存,96孔板在室溫保存。所有組分保質(zhì)期為1年,試劑一旦開(kāi)封,在4度保存時(shí)間為1個(gè)月。
配套試劑
贈(zèng)品
增值服務(wù)
相關(guān)產(chǎn)品
編號(hào) | 適用物種:Mus musculus (Mouse,小鼠) | 應(yīng)用(僅供研究使用,不用于臨床診斷!) |
RPP155Mu01 | 無(wú)翅型MMTV整合位點(diǎn)家族成員3A(WNT3A)重組蛋白 | Positive Control; Immunogen; SDS-PAGE; WB. |
RPP155Mu02 | 無(wú)翅型MMTV整合位點(diǎn)家族成員3A(WNT3A)重組蛋白 | Positive Control; Immunogen; SDS-PAGE; WB. |
PAP155Mu01 | 無(wú)翅型MMTV整合位點(diǎn)家族成員3A(WNT3A)多克隆抗體 | WB; IHC; ICC; IP. |
PAP155Mu02 | 無(wú)翅型MMTV整合位點(diǎn)家族成員3A(WNT3A)多克隆抗體 | WB; IHC; ICC; IP. |
SEP155Mu | 無(wú)翅型MMTV整合位點(diǎn)家族成員3A(WNT3A)檢測(cè)試劑盒(酶聯(lián)免疫吸附試驗(yàn)法) | Enzyme-linked immunosorbent assay for Antigen Detection. |
HEP155Mu | 無(wú)翅型MMTV整合位點(diǎn)家族成員3A(WNT3A)檢測(cè)試劑盒(酶聯(lián)免疫吸附試驗(yàn)法,高敏型) | Enzyme-linked immunosorbent assay for Antigen Detection. |
LMP155Mu | 無(wú)翅型MMTV整合位點(diǎn)家族成員3A(WNT3A)等多因子檢測(cè)試劑盒(流式熒光發(fā)光法) | FLIA Kit for Antigen Detection. |
KSP155Mu01 | 無(wú)翅型MMTV整合位點(diǎn)家族成員3A(WNT3A)檢測(cè)試劑盒DIY材料(酶聯(lián)免疫吸附試驗(yàn)法) | Main materials for "Do It (ELISA Kit) Yourself". |
參考文獻(xiàn)
雜志 | 參考文獻(xiàn) |
Medical Oncology | Wls promotes the proliferation of breast cancer cells via Wnt signaling [Pubmed:25801232] |
J Histochem Cytochem | Wnt3a Mediates the Inhibitory Effect of Hyperoxia on the Transdifferentiation of AECIIs to AECIs [PubMed: 26209081] |
PloS one | Gelatin device for the delivery of growth factors involved in endochondral ossification [pubmed:28380024] |
Life Sciences | Hepatoprotective effect of hesperidin in hepatocellular carcinoma: Involvement of Wnt signaling pathways. [pubmed:28754618] |
Oxidative Medicine and Cellular Longevity | Secreted Frizzled-Related Protein-2 Inhibits Doxorubicin Induced Apoptosis Mediated Through the Akt-mTOR Pathway in Soleus Muscle [Pubmed:30151071] |
The Showa University Journal of Medical Sciences | Eribulin Treatment Induces High Expression of miR-195 and Inactivates the Wnt/β-catenin Signaling Pathway in Triple-negative Breast Cancer [Doi: 10.15369/sujms.30.359] |
Journal of Cancer | Oncogenic Wnt3a: A Candidate Specific Marker and Novel Molecular Target for Hepatocellular Carcinoma [Pubmed: 31737122] |
CANCER LETTERS | Knock down of BMSC-derived Wnt3a or its antagonist analogs attenuate colorectal carcinogenesis induced by chronic Fusobacterium nucleatum infection [Pubmed: 32920199] |
Biomaterials | An improved osseointegration of metal implants by pitavastatin loaded multilayer films with osteogenic and angiogenic properties [34823885] |
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