順烏頭酸酶1(ACO1)多克隆抗體
Polyclonal Antibody to Aconitase 1 (ACO1)
IREB1; IRP1; IREBP; Iron regulatory protein 1; Iron-responsive element-binding protein 1; Cytoplasmic aconitate hydratase; Citrate hydro-lyase; Ferritin repressor protein
- 編號PAJ683Hu02
- 物種Homo sapiens (Human，人) 相同的名稱，不同的物種。
- 來源多抗制備
- 宿主兔
- 效價-
- Ig類型 IgG
- 純化方式抗原特異性親和純化
- 標記物無標記物
- 免疫原 RPJ683Hu02-順烏頭酸酶1(ACO1)重組蛋白
- 緩沖液成份0.01M 磷酸鹽緩沖液（pH7.4，containing 0.05% Proclin-300，50% glycerol）
- 性狀液體
- 濃度500μg/mL
- 且適物種Mus musculus (Mouse，小鼠), Rattus norvegicus (Rat，大鼠), Sus scrofa; Porcine (Pig，豬)
- 應用WB; ICC; IHC-P; IHC-F; ELISA; FCM
如果抗體需用于流式細胞術，請參見流式抗體。 - 下載 英文說明書 中文說明書
- 規(guī)格 20μl100μl 200μl 1ml 10ml
- 價格 ¥ 630 ¥ 1470 ¥ 2100 ¥ 5250 ¥ 21000
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產品包裝(模擬)
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產品包裝(模擬)
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DAB staining on IHC-P; Samples: Human Kidney Tissue; Primary Ab: 10μg/ml Rabbit Anti-Human ACO1 Antibody Second Ab: 2μg/mL?HRP-Linked Caprine Anti-Rabbit IgG Polyclonal Antibody (Catalog: SAA544Rb19)
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DAB staining on IHC-P;
Samples: Human Lung cancer Tissue;
Primary Ab: 10μg/ml Rabbit Anti-Human ACO1 Antibody
Second Ab: 2μg/mL?HRP-Linked Caprine Anti-Rabbit IgG Polyclonal Antibody
(Catalog: SAA544Rb19) -
DAB staining on IHC-P;
Samples: Human Liver Tissue;
Primary Ab: 20μg/ml Rabbit Anti-Human ACO1 Antibody
Second Ab: 2μg/mL?HRP-Linked Caprine Anti-Rabbit IgG Polyclonal Antibody
(Catalog: SAA544Rb19)
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Figure. Western Blot; Sample: Recombinant ACO1, Human.
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Western Blot; Sample: Porcine Liver lysate;
Primary Ab: 2μg/ml Rabbit Anti-Human ACO1 Antibody
Second Ab: 0.2μg/mL?HRP-Linked Caprine Anti-Rabbit IgG Polyclonal Antibody
(Catalog: SAA544Rb19) -
Western Blot; Sample: Mouse Liver lysate;
Primary Ab: 2μg/ml Rabbit Anti-Human ACO1 Antibody
Second Ab: 0.2μg/mL?HRP-Linked Caprine Anti-Rabbit IgG Polyclonal Antibody
(Catalog: SAA544Rb19) -
Western Blot; Sample: Human HepG2 cell lysate;
Primary Ab: 2μg/ml Rabbit Anti-Human ACO1 Antibody
Second Ab: 0.2μg/mL?HRP-Linked Caprine Anti-Rabbit IgG Polyclonal Antibody
(Catalog: SAA544Rb19) -
Western Blot; Sample: Human 293T cell lysate;
Primary Ab: 2μg/ml Rabbit Anti-Human ACO1 Antibody
Second Ab: 0.2μg/mL?HRP-Linked Caprine Anti-Rabbit IgG Polyclonal Antibody
(Catalog: SAA544Rb19) -
Western Blot; Sample: Porcine Kidney lysate;
Primary Ab: 2μg/ml Rabbit Anti-Human ACO1 Antibody
Second Ab: 0.2μg/mL?HRP-Linked Caprine Anti-Rabbit IgG Polyclonal Antibody
(Catalog: SAA544Rb19) -
Human SiHa cell was fixed with 2% paraformaldehyde (10 min) , permeabilised with 0.1% BSA-Triton X-100,then stained with 20μg/ml rabbit Anti-human ACO1 Polyclonal Antibody (Catalog PAJ683Hu02, red histogram) or Isotype control antibody (Catalog IS067-Rb01, green histogram), followed by 1μg/ml FITC-conjugated Anti-rabbit IgG Secondary Antibody (Catalog SAA544Rb18).
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通過ISO 9001、ISO 13485質量體系認證
特異性
該抗體是針對ACO1的兔多克隆抗體。在免疫組織化學染色和免疫印跡實驗中能識別ACO1。
用法
Western blotting: 1-5μg/mL
Immunocytochemistry in formalin fixed cells: 5-20μg/mL
Immunohistochemistry in formalin fixed frozen section: 5-20μg/mL
Immunohistochemistry in paraffin section: 5-20μg/mL
Enzyme-linked Immunosorbent Assay: 0.05-2μg/mL
Flow cytometry:20μg/ml;
Optimal working dilutions must be determined by end user.
儲存
經常使用則4°C保存。-20°C保存不超過兩年。避免反復凍融。
穩(wěn)定性
熱穩(wěn)定性以損失率顯示。損失率是由加速降解試驗決定，具體方法如下：在37°C孵育48小時，沒有顯著的降解或者沉淀產生。保質期內，在適當?shù)臈l件下存儲，損失率低于5%。
贈品
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相關產品
| 編號 | 適用物種：Homo sapiens (Human，人) | 應用(僅供研究使用，不用于臨床診斷！) |
| RPJ683Hu02 | 順烏頭酸酶1(ACO1)重組蛋白 | Positive Control; Immunogen; SDS-PAGE; WB. |
| RPJ683Hu03 | 順烏頭酸酶1(ACO1)重組蛋白 | Positive Control; Immunogen; SDS-PAGE; WB. |
| RPJ683Hu04 | 順烏頭酸酶1(ACO1)重組蛋白 | Positive Control; Immunogen; SDS-PAGE; WB. |
| RPJ683Hu01 | 順烏頭酸酶1(ACO1)重組蛋白 | Positive Control; Immunogen; SDS-PAGE; WB. |
| PAJ683Hu04 | 順烏頭酸酶1(ACO1)多克隆抗體 | WB; ICC; IHC-P; IHC-F; ELISA. |
| PAJ683Hu02 | 順烏頭酸酶1(ACO1)多克隆抗體 | WB; ICC; IHC-P; IHC-F; ELISA; FCM |
| PAJ683Hu01 | 順烏頭酸酶1(ACO1)多克隆抗體 | WB; ICC; IHC-P; IHC-F; ELISA; FCM |
| PAJ683Hu03 | 順烏頭酸酶1(ACO1)多克隆抗體 | WB; IHC; ICC; IP; FCM |
| LAJ683Hu71 | 順烏頭酸酶1(ACO1)多克隆抗體(生物素標記) | WB; IHC; ICC. |
| SEJ683Hu | 順烏頭酸酶1(ACO1)檢測試劑盒(酶聯(lián)免疫吸附試驗法) | Enzyme-linked immunosorbent assay for Antigen Detection. |
| LMJ683Hu | 順烏頭酸酶1(ACO1)等多因子檢測試劑盒(流式熒光發(fā)光法) | FLIA Kit for Antigen Detection. |
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