谷丙轉(zhuǎn)氨酶(ALT)多克隆抗體
Polyclonal Antibody to Alanine Aminotransferase (ALT)
GPT; ALAT; SGPT; AAT1; GPT1; Serum Glutamic Pyruvic Transaminase; Alanine Transaminase; Glutamate pyruvate transaminase 1; Glutamic--alanine transaminase 1
- 編號PAA207Hu02
- 物種Homo sapiens (Human，人) 相同的名稱，不同的物種。
- 來源多抗制備
- 宿主兔
- 效價-
- Ig類型 IgG
- 純化方式抗原特異性親和純化
- 標記物無標記物
- 免疫原 RPA207Hu02-谷丙轉(zhuǎn)氨酶(ALT)重組蛋白
- 緩沖液成份0.01M 磷酸鹽緩沖液（pH7.4，containing 0.05% Proclin-300，50% glycerol）
- 性狀液體
- 濃度0.5mg/ml
- 且適物種Mus musculus (Mouse，小鼠), Rattus norvegicus (Rat，大鼠)
- 應用WB; IHC; ICC; IP.
如果抗體需用于流式細胞術，請參見流式抗體。 - 下載 英文說明書 中文說明書
- 規(guī)格 20μl100μl 200μl 1ml 10ml
- 價格 ¥ 569 ¥ 1327 ¥ 1896 ¥ 4740 ¥ 18960
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產(chǎn)品包裝(模擬)
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產(chǎn)品包裝(模擬)
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DAB staining on IHC-P; Samples: Human Liver Tissue; Primary Ab: 20μg/ml Rabbit Anti-Human ALT Antibody Second Ab: 2μg/mL?HRP-Linked Caprine Anti-Rabbit IgG Polyclonal Antibody (Catalog: SAA544Rb19)
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Vector Red staining on IHC-P;
Samples: Human Kidney Tissue;
Primary Ab: 15μg/ml Rabbit Anti-Human ALT Antibody
Second Ab: 2μg/mL?HRP-Linked Caprine Anti-Rabbit IgG Polyclonal Antibody -
Vector Red staining on IHC-P;
Samples: Human Liver Tissue;
Primary Ab: 15μg/ml Rabbit Anti-Human ALT Antibody
Second Ab: 2μg/mL?HRP-Linked Caprine Anti-Rabbit IgG Polyclonal Antibody -
DAB staining on IHC-P;
Sample: Human Liver cancer Tissue;
Primary Ab: 20μg/ml Rabbit Anti-Human ALT Antibody
Second Ab: 2μg/mL?HRP-Linked Caprine Anti-Rabbit IgG Polyclonal Antibody
(Catalog: SAA544Rb19)
Secondary antibody only control: Used PBS instead of primary antibody, Second Ab: 2μg/mL?HRP-Linked Caprine Anti-Rabbit IgG Polyclonal Antibody
(Catalog: SAA544Rb19) -
DAB staining on IHC-P;
Sample: Human Kidney Tissue;
Primary Ab: 20μg/ml Rabbit Anti-Human ALT Antibody
Second Ab: 2μg/mL?HRP-Linked Caprine Anti-Rabbit IgG Polyclonal Antibody
(Catalog: SAA544Rb19)
Secondary antibody only control: Used PBS instead of primary antibody, Second Ab: 2μg/mL?HRP-Linked Caprine Anti-Rabbit IgG Polyclonal Antibody
(Catalog: SAA544Rb19) -
DAB staining on IHC-P;
Sample: Rat Liver Tissue;
Primary Ab: 10μg/ml Rabbit Anti-Human ALT Antibody
Second Ab: 2μg/mL?HRP-Linked Caprine Anti-Rabbit IgG Polyclonal Antibody
(Catalog: SAA544Rb19)
Secondary antibody only control: Used PBS instead of primary antibody, Second Ab: 2μg/mL?HRP-Linked Caprine Anti-Rabbit IgG Polyclonal Antibody
(Catalog: SAA544Rb19) -
DAB staining on IHC-P;
Sample: Human Breast cancer Tissue
Primary Ab: 20μg/ml Rabbit Anti-Human ALT Antibody
Control: Used PBS instead of primary antibody
Second Ab: 2μg/ml HRP-Linked Caprine Anti-Rabbit IgG Polyclonal Antibody
(Catalog: SAA544Rb19) -
DAB staining on IHC-P;
Sample: Human Liver Tissue
Primary Ab: 20μg/ml Rabbit Anti-Human ALT Antibody
Control: Used PBS instead of primary antibody
Second Ab: 2μg/ml HRP-Linked Caprine Anti-Rabbit IgG Polyclonal Antibody
(Catalog: SAA544Rb19) -
DAB staining on IHC-P;
Sample: Human Kidney Tissue
Primary Ab: 20μg/ml Rabbit Anti-Human ALT Antibody
Control: Used PBS instead of primary antibody
Second Ab: 2μg/ml HRP-Linked Caprine Anti-Rabbit IgG Polyclonal Antibody
(Catalog: SAA544Rb19)
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Figure. Western Blot; Sample: Recombinant ALT, Human.
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Western Blot; Sample: Mouse Liver lysate; <br/>Primary Ab: 2μg/ml Rabbit Anti-Human ALT Antibody<br/>Second Ab: 0.2μg/mL?HRP-Linked Caprine Anti-Rabbit IgG Polyclonal Antibody<br/>(Catalog: SAA544Rb19) Selected
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Western Blot; Sample: Mouse Cerebrum lysate;
Primary Ab: 2μg/ml Rabbit Anti-Human ALT Antibody
Second Ab: 0.2μg/mL HRP-Linked Caprine Anti-Rabbit IgG Polyclonal Antibody
(Catalog: SAA544Rb19) -
Western Blot; Sample: Lane1: Rat Heart lysate; Lane2: Rat Liver lysate; Lane3: Rat Kidney lysate; Lane4: Rat Cerebrum lysate; Lane5: Rat Skeletal muscle lysate<br/>Primary Ab: 0.2μg/ml Rabbit Anti-Human ALT Antibody<br/>Second Ab: 0.2μg/mL HRP-Linked Caprine Anti-Rabbit IgG Polyclonal Antibody<br/>(Catalog: SAA544Rb19) Selected
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Western Blot; Sample: Mouse Kidney lysate;
Primary Ab: 2μg/ml Rabbit Anti-Human ALT Antibody
Second Ab: 0.2μg/mL HRP-Linked Caprine Anti-Rabbit IgG Polyclonal Antibody
(Catalog: SAA544Rb19)
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通過ISO 9001、ISO 13485質(zhì)量體系認證
特異性
該抗體是針對ALT的兔多克隆抗體。在免疫組織化學染色和免疫印跡實驗中能識別ALT。
用法
Western blotting: 0.01-2μg/mL;
Immunohistochemistry: 5-20μg/mL;
Immunocytochemistry: 5-20μg/mL;
Optimal working dilutions must be determined by end user.
儲存
經(jīng)常使用則4°C保存。-20°C保存不超過兩年。避免反復凍融。
穩(wěn)定性
熱穩(wěn)定性以損失率顯示。損失率是由加速降解試驗決定，具體方法如下：在37°C孵育48小時，沒有顯著的降解或者沉淀產(chǎn)生。保質(zhì)期內(nèi)，在適當?shù)臈l件下存儲，損失率低于5%。
贈品
增值服務
相關產(chǎn)品
| 編號 | 適用物種：Homo sapiens (Human，人) | 應用(僅供研究使用，不用于臨床診斷！) |
| RPA207Hu02 | 谷丙轉(zhuǎn)氨酶(ALT)重組蛋白 | Positive Control; Immunogen; SDS-PAGE; WB. |
| RPA207Hu03 | 谷丙轉(zhuǎn)氨酶(ALT)重組蛋白 | Positive Control; Immunogen; SDS-PAGE; WB. |
| RPA207Hu01 | 谷丙轉(zhuǎn)氨酶(ALT)重組蛋白 | Positive Control; Immunogen; SDS-PAGE; WB. |
| PAA207Hu01 | 谷丙轉(zhuǎn)氨酶(ALT)多克隆抗體 | WB; IHC; ICC; IP. |
| PAA207Hu03 | 谷丙轉(zhuǎn)氨酶(ALT)多克隆抗體 | WB; ICC; IHC-P; IHC-F; ELISA. |
| PAA207Hu02 | 谷丙轉(zhuǎn)氨酶(ALT)多克隆抗體 | WB; IHC; ICC; IP. |
| LAA207Hu71 | 谷丙轉(zhuǎn)氨酶(ALT)多克隆抗體(生物素標記) | WB; IHC; ICC. |
| LAA207Hu81 | 谷丙轉(zhuǎn)氨酶(ALT)多克隆抗體(異硫氰酸熒光素標記) | WB; IHC; ICC; IF. |
| MAA207Hu21 | 谷丙轉(zhuǎn)氨酶(ALT)單克隆抗體 | WB; IHC |
| MAA207Hu24 | 谷丙轉(zhuǎn)氨酶(ALT)單克隆抗體 | WB; IHC; ICC; IP. |
| MAA207Hu23 | 谷丙轉(zhuǎn)氨酶(ALT)單克隆抗體 | WB; IHC; ICC; IP. |
| MAA207Hu25 | 谷丙轉(zhuǎn)氨酶(ALT)單克隆抗體 | WB; IHC; ICC; IP. |
| MAA207Hu22 | 谷丙轉(zhuǎn)氨酶(ALT)單克隆抗體 | WB,IHC |
| MAA207Hu27 | 谷丙轉(zhuǎn)氨酶(ALT)單克隆抗體 | WB; IHC; ICC; IP. |
| MAA207Hu29 | 谷丙轉(zhuǎn)氨酶(ALT)單克隆抗體 | WB; IHC; ICC; IP. |
| LAA207Hu72 | 谷丙轉(zhuǎn)氨酶(ALT)單克隆抗體(生物素標記) | WB; IHC; ICC. |
| MEA207Hu | 谷丙轉(zhuǎn)氨酶(ALT)檢測試劑盒(酶聯(lián)免疫吸附試驗法,小樣本) | Enzyme-linked immunosorbent assay for Antigen Detection. |
| SEA207Hu | 谷丙轉(zhuǎn)氨酶(ALT)檢測試劑盒(酶聯(lián)免疫吸附試驗法) | Enzyme-linked immunosorbent assay for Antigen Detection. |
| LMA207Hu | 谷丙轉(zhuǎn)氨酶(ALT)等多因子檢測試劑盒(流式熒光發(fā)光法) | FLIA Kit for Antigen Detection. |
參考文獻
| 雜志 | 參考文獻 |
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| Endocrinology. | Additive effects of nicotine and high-fat diet on hepatic steatosis in male mice. [PubMed: 23093702] |
| Kidney International | Adrenocorticotropic hormone ameliorates acute kidney injury by steroidogenic-dependent and -independent mechanisms [PubMed: PMC3612362] |
| Proceedings of the National Academy of Sciences | Pathogenesis of emerging severe fever with thrombocytopenia syndrome virus in C57/BL6 mouse model [PubMed: PMC3382536] |
| African Journal of Pharmacy and Pharmacology | Protective effect of Panax ginseng against N-acetyl-p-aminophenol-induced hepatotoxicity in rats [Academicjournals: Source] |
| Kidney International | Adrenocorticotropic hormone ameliorates acute kidney injury by steroidogenic-dependent and-independent mechanisms [Pubmed: 23325074] |
| European Journal of Pharmacology | Protective effect of Et-1 receptor antagonist bosentan on paracetamol induced acute liver toxicity in rats [ScienceDirect: S0014299914000235] |
| Eur J Nutr. | Bifidobacterium pseudocatenulatum CECT7765 promotes a TLR2-dependent anti-inflammatory response in intestinal lymphocytes from mice with cirrhosis [Pubmed:25657013] |
| Experimental and Molecular Pathology | Resolvin E1 and chemerin C15 peptide do not improve rodent non-alcoholic steatohepatitis [Pubmed:25681756] |
| Gastroenterol Res Pract | Assessing the Effect of Leptin on Liver Damage in Case of Hepatic Injury Associated with Paracetamol Poisoning [PubMed: 26697061] |
| FNG & Bilim T?p Dergisi | The effects of subacute deltamethrin exposure on rat liver: histochemical, immunohistochemical, and biochemical study [xmlui:11446] |
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| DARU?Journal of Pharmaceutical Sciences | Effects of developmental exposure to silver in ionic and nanoparticle form: A study in rats [pubmed:27716437] |
| Oncotarget | Dihydromyricetin?protects?against?liver?ischemia/reperfusion?induced?apoptosis?via?activation?ofFOXO3a-mediated?autophagy. [pubmed:27793014] |
| Scientific Reports | Adiponectin?protects?the?rats?liver?against?chronic?intermittent?hypoxia?induced?injury?throughAMP-activated?protein?kinase?pathway. [pubmed:27678302] |
| ?Biochimica et Biophysica Acta (BBA)-General Subjects | Modulation of gut microbiota contributes to curcumin-mediated attenuation of hepatic steatosis in rats [pubmed:28341485] |
| Gastroenterology Research and Practice | Antifibrotic Effect of Lactulose on a Methotrexate-Induced Liver Injury Model [pubmed:28912805] |
| PLoS Pathogens | Alcohol-associated intestinal dysbiosis impairs pulmonary host defense against Klebsiella pneumoniae. [pubmed:28604843] |
| FEBS?Open?Bio | A critical role of hepatitis B virus polymerase in cirrhosis, hepatocellular carcinoma, and steatosis [Pubmed:29321963] |
| Rolle von CMKLR1 und dessen Liganden bei der nicht-alkoholischen Fettlebererkrankung [Regensburg:Source] | |
| Cumhuriyet Medical Journal | The valuable effects of potent antioxidant curcumin in cisplatin induced liver and kidney injury [DOI:Source] |
| BMC?Complementary and Alternative?Medicine? | Hepatoprotective activity of Erythrina× neillii leaf extract and characterization of its phytoconstituents [Pubmed: 28095910] |
| Scientific Reports | Brg1 promotes liver regeneration after partial hepatectomy via regulation of cell cycle [Pubmed: 30787318] |
| Brain Behavior and Immunity | Corticosterone-mediated microglia activation affects dendritic spine plasticity and motor learning functions in minimal hepatic encephalopathy [Pubmed: 31437533] |
| Biomed Research International | Liraglutide Attenuates Nonalcoholic Fatty Liver Disease by Modulating Gut Microbiota in Rats Administered a High-Fat Diet [Pubmed: 32149099] |
| Frontiers in Immunology | CD47 Deficiency in Mice Exacerbates Chronic Fatty Diet-Induced Steatohepatitis Through Its Role in Regulating Hepatic Inflammation and Lipid Metabolism [Pubmed: 32158445] |
| Nanomaterials | Biological Safety and Biodistribution of Chitosan Nanoparticles [Pubmed: 32340313] |
| European Journal of Biology | Effects of Tempol in Lipopolysaccharide-Induced Liver Injury [] |
| Pathogens | Antimalarial Effect of the Total Glycosides of the Medicinal Plant, Ranunculus japonicus [33925018] |
| Gastroenterol Res Pract | An Experimental Study: Benefits of Digoxin on Hepatotoxicity Induced by Methotrexate Treatment [34804155] |
| Int Immunopharmacol | Taraxasterol mitigates Con A-induced hepatitis in mice by suppressing interleukin-2 expression and its signaling in T lymphocytes [34848154] |
| J Magn Reson Imaging | Water Specific MRI T1 Mapping for Evaluating Liver Inflammation Activity Grades in Rats With Methionine‐Choline‐Deficient Diet‐Induced Nonalcoholic Fatty Liver?… [Pubmed:35212074] |
| Medcomm | Blockade of mIL‐6R alleviated lipopolysaccharide‐induced systemic inflammatory response syndrome by suppressing NF‐κB‐mediated Ccl2 expression and?… [Pubmed:35548710] |
| International Immunopharmacology | Dimethyl fumarate ameliorates autoimmune hepatitis in mice by blocking NLRP3 inflammasome activation [Pubmed:35605433] |
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