CD276分子(CD276)多克隆抗體
Polyclonal Antibody to Cluster Of Differentiation 276 (CD276)
B7-H3; B7H3; CD276 Molecule; B7 homolog 3; Costimulatory molecule
- 編號PAA781Hu01
- 物種Homo sapiens (Human，人) 相同的名稱，不同的物種。
- 來源多抗制備
- 宿主兔
- 效價-
- Ig類型 IgG
- 純化方式抗原特異性親和純化
- 標記物無標記物
- 免疫原 RPA781Hu01-CD276分子(CD276)重組蛋白
- 緩沖液成份0.01M 磷酸鹽緩沖液（pH7.4，containing 0.05% Proclin-300，50% glycerol）
- 性狀液體
- 濃度0.5mg/mL
- 且適物種-
- 應(yīng)用WB; IHC; FCM
如果抗體需用于流式細胞術(shù)，請參見流式抗體。 - 下載 英文說明書 中文說明書
- 規(guī)格 20μl100μl 200μl 1ml 10ml
- 價格 ¥ 569 ¥ 1327 ¥ 1896 ¥ 4740 ¥ 18960
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產(chǎn)品包裝(模擬)
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產(chǎn)品包裝(模擬)
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DAB staining on IHC-P; Samples: Human Liver cancer Tissue; Primary Ab: 10μg/ml Rabbit Anti-Human CD276 Antibody Second Ab: 2μg/mL?HRP-Linked Caprine Anti-Rabbit IgG Polyclonal Antibody (Catalog: SAA544Rb19)
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Vector Red staining on IHC-P;
Samples: Human Placenta Tissue;
Primary Ab: 10μg/ml Rabbit Anti-Human CD276 Antibody
Second Ab: 2μg/mL?HRP-Linked Caprine Anti-Rabbit IgG Polyclonal Antibody -
DAB staining on IHC-P;
Sample: Human Prostate Tissue
Primary Ab: 10μg/ml Rabbit Anti-Human CD276 Antibody
Control: Used PBS instead of primary antibody
Second Ab: 2μg/ml HRP-Linked Caprine Anti-Rabbit IgG Polyclonal Antibody
(Catalog: SAA544Rb19)
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Figure. Western Blot; Sample: Recombinant CD276, Human.
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Western Blot; Sample: Lane1: 293T cell lysate; Lane2: PC3 cell lysate; Lane3: MCF7 cell lysate
Primary Ab: 3μg/ml Rabbit Anti-Human CD276 Antibody
Second Ab: 0.2μg/mL HRP-Linked Caprine Anti-Rabbit IgG Polyclonal Antibody
(Catalog: SAA544Rb19) -
Knockout Varification:
Lane 1: Wild-type 293T cell lysate;
Lane 2: CD276 knockout 293T cell lysate;
Predicted MW: 57,53,33kd
Observed MW: 100kd
Primary Ab: 3μg/ml Rabbit Anti-Human CD276 Antibody
Second Ab: 0.2μg/mL?HRP-Linked Caprine Anti-Rabbit IgG Polyclonal Antibody
(Catalog: SAA544Rb19) -
ChoK1 transfected with human CD276 (Stable transfection) was stained with rabbit Anti-human CD276 Polyclonal Antibody (Catalog # PAA781Hu01), filled red histogram) or Isotype control antibody (Catalog # IS067-Rb01, filled blue histogram), followed by CY5-conjugated Anti-rabbit IgG Secondary Antibody (Catalog # SAA544Rb11). Cells were fixed with 4% paraformaldehyde and permeabilized with 0.1% Triton X-100.
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通過ISO 9001、ISO 13485質(zhì)量體系認證
特異性
該抗體是針對CD276的兔多克隆抗體。在免疫組織化學(xué)染色和免疫印跡實驗中能識別CD276。
用法
Western blotting: 0.01-3μg/mL;
Immunohistochemistry: 5-30μg/mL;
For flow cytometry: 0.5-2 μg per 10^6 cells in 100 μl volume;
Optimal working dilutions must be determined by end user.
儲存
經(jīng)常使用則4°C保存。-20°C保存不超過兩年。避免反復(fù)凍融。
穩(wěn)定性
熱穩(wěn)定性以損失率顯示。損失率是由加速降解試驗決定，具體方法如下：在37°C孵育48小時，沒有顯著的降解或者沉淀產(chǎn)生。保質(zhì)期內(nèi)，在適當?shù)臈l件下存儲，損失率低于5%。
贈品
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相關(guān)產(chǎn)品
| 編號 | 適用物種：Homo sapiens (Human，人) | 應(yīng)用(僅供研究使用，不用于臨床診斷！) |
| APA781Hu01 | CD276分子(CD276)活性蛋白 | Cell?culture;?Activity?Assays. |
| RPA781Hu01 | CD276分子(CD276)重組蛋白 | Positive Control; Immunogen; SDS-PAGE; WB. |
| PAA781Hu01 | CD276分子(CD276)多克隆抗體 | WB; IHC; FCM |
| FAA781Hu01 | 抗CD276分子(CD276)多克隆抗體 | Flow cytometry. |
| MAA781Hu22 | CD276分子(CD276)單克隆抗體 | WB |
| MAA781Hu21 | CD276分子(CD276)單克隆抗體 | WB; IHC; ICC; IP. |
| SEA781Hu | CD276分子(CD276)檢測試劑盒(酶聯(lián)免疫吸附試驗法) | Enzyme-linked immunosorbent assay for Antigen Detection. |
| LMA781Hu | CD276分子(CD276)等多因子檢測試劑盒(流式熒光發(fā)光法) | FLIA Kit for Antigen Detection. |
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