周期素依賴性激酶抑制因子2A(CDKN2A)多克隆抗體
Polyclonal Antibody to Cyclin Dependent Kinase Inhibitor 2A (CDKN2A)
P16; ARF; CDK4I; CMM2; INK4; INK4a; MLM; MTS1; TP16; P14; P14ARF; P16INK4a; P19; Melanoma P16; Cyclin-dependent kinase 4 inhibitor A; Multiple tumor suppressor 1
- 編號PAA794Hu01
- 物種Homo sapiens (Human，人) 相同的名稱，不同的物種。
- 來源多抗制備
- 宿主兔
- 效價-
- Ig類型 IgG
- 純化方式抗原特異性親和純化
- 標(biāo)記物無標(biāo)記物
- 免疫原 RPA794Hu01-周期素依賴性激酶抑制因子2A(CDKN2A)重組蛋白
- 緩沖液成份0.01M 磷酸鹽緩沖液（pH7.4，containing 0.05% Proclin-300，50% glycerol）
- 性狀液體
- 濃度0.5mg/ml
- 且適物種-
- 應(yīng)用WB; IHC; ICC/IF;FCM
如果抗體需用于流式細胞術(shù)，請參見流式抗體。 - 下載 英文說明書 中文說明書
- 規(guī)格 20μl100μl 200μl 1ml 10ml
- 價格 ¥ 569 ¥ 1327 ¥ 1896 ¥ 4740 ¥ 18960
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產(chǎn)品包裝(模擬)
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產(chǎn)品包裝(模擬)
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DAB staining on IHC-P; Samples: Human Liver Tissue; Primary Ab: 20μg/ml Rabbit Anti-Human CDKN2A Antibody Second Ab: 2μg/mL?HRP-Linked Caprine Anti-Rabbit IgG Polyclonal Antibody (Catalog: SAA544Rb19)
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DAB staining on IHC-P;
Sample: Rat Cerebrum Tissue
Primary Ab: 10μg/ml Rabbit Anti-Human CDKN2A Antibody
Control: Used PBS instead of primary antibody
Second Ab: 2μg/ml HRP-Linked Caprine Anti-Rabbit IgG Polyclonal Antibody
(Catalog: SAA544Rb19) -
FITC staining on IF;
Samples: Human MCF7 cell;
Primary Ab: 20μg/ml Rabbit Anti-Human CDKN2A Antibody
Second Ab: 0.75μg/ml FITC-Linked Caprine Anti-Rabbit IgG Polyclonal Antibody
(Catalog: SAA544Rb18) -
FITC staining on IF;
Samples: Human Hela cell;
Primary Ab: 20μg/ml Rabbit Anti-Human CDKN2A Antibody
Second Ab: 1μg/ml FITC-Linked Caprine Anti-Rabbit IgG Polyclonal Antibody
(Catalog: SAA544Rb18) -
AF488 staining on IF;
Sample: Hela cell
Primary Ab: 20μg/ml Rabbit Anti-Human CDKN2A Antibody
Second Ab: 2μg/ml AF488-Linked Caprine Anti-Rabbit IgG Polyclonal Antibody
(Catalog: SAA544Rb11)
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Figure. Western Blot; Sample: Recombinant CDKN2A, Human.
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Western Blot; Samples: Lane1: Hela cell lysate; Lane2: MCF7 cell lysate; Lane3: HepG2 cell lysate;
Primary Ab: 0.05μg/ml Rabbit Anti-Human CDKN2A Antibody
Second Ab: 0.2μg/ml HRP-Linked Caprine Anti-Rabbit IgG Polyclonal Antibody
(Catalog: SAA544Rb19) -
WB圖
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WB圖
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HepG2 human hepatocellular carcinoma cell line was stained with rabbit Anti-human CDKN2A Polyclonal Antibody (Catalog # PAA794Hu01, filled red histogram) or Isotype control antibody (Catalog # IS067-Rb01, filled green histogram), followed by APC-conjugated Anti-rabbit IgG Secondary Antibody (Catalog # SAA544Rb15). Cells were fixed with 4% paraformaldehyde and permeabilized with 0.1% Triton X-100.
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Human Hela cell was fixed with 2% paraformaldehyde (10 min) , permeabilised with 0.1% BSA-Triton X-100,then stained with 20μg/ml rabbit Anti-human CDKN2A Polyclonal Antibody (Catalog PAA794Hu01, red histogram) or Isotype control antibody (Catalog IS067-Rb01, green histogram), followed by 1μg/ml FITC-conjugated Anti-rabbit IgG Secondary Antibody (Catalog SAA544Rb18).
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通過ISO 9001、ISO 13485質(zhì)量體系認證
特異性
該抗體是針對CDKN2A的兔多克隆抗體。在免疫組織化學(xué)染色和免疫印跡實驗中能識別CDKN2A。
用法
Western blotting: 0.01-2μg/mL;
Immunohistochemistry: 5-20μg/mL;
Immunocytochemistry: 5-20μg/mL;
Flow cytometry:20μg/ml;
Optimal working dilutions must be determined by end user.
儲存
經(jīng)常使用則4°C保存。-20°C保存不超過兩年。避免反復(fù)凍融。
穩(wěn)定性
熱穩(wěn)定性以損失率顯示。損失率是由加速降解試驗決定，具體方法如下：在37°C孵育48小時，沒有顯著的降解或者沉淀產(chǎn)生。保質(zhì)期內(nèi)，在適當(dāng)?shù)臈l件下存儲，損失率低于5%。
贈品
增值服務(wù)
相關(guān)產(chǎn)品
| 編號 | 適用物種：Homo sapiens (Human，人) | 應(yīng)用(僅供研究使用，不用于臨床診斷！) |
| RPA794Hu01 | 周期素依賴性激酶抑制因子2A(CDKN2A)重組蛋白 | Positive Control; Immunogen; SDS-PAGE; WB. |
| PAA794Hu01 | 周期素依賴性激酶抑制因子2A(CDKN2A)多克隆抗體 | WB; IHC; ICC/IF;FCM |
| FAA794Hu01 | 抗周期素依賴性激酶抑制因子2A(CDKN2A)多克隆抗體 | Flow cytometry. |
| MAA794Hu22 | 周期素依賴性激酶抑制因子2A(CDKN2A)單克隆抗體 | WB; IHC; ICC; IP. |
| MEA794Hu | 周期素依賴性激酶抑制因子2A(CDKN2A)檢測試劑盒(酶聯(lián)免疫吸附試驗法,小樣本) | Enzyme-linked immunosorbent assay for Antigen Detection. |
| SEA794Hu | 周期素依賴性激酶抑制因子2A(CDKN2A)檢測試劑盒(酶聯(lián)免疫吸附試驗法) | Enzyme-linked immunosorbent assay for Antigen Detection. |
| LMA794Hu | 周期素依賴性激酶抑制因子2A(CDKN2A)等多因子檢測試劑盒(流式熒光發(fā)光法) | FLIA Kit for Antigen Detection. |
參考文獻
| 雜志 | 參考文獻 |
| Biochemical genetics | Comparison of Selected Protein Levels in Tumour and Surgical Margin in a Group of Patients with Oral Cavity Cancer [pubmed:28421310] |
| European?Journal of Pharmaceutical?Sciences | Development of core-shell nanocarrier system for augmenting piperine cytotoxic activity against human brain cancer cell line [Pubmed:29597041] |
| Clinical and Experimental Medicine | Senescence-associated miR-34a and miR-126 in middle-aged Indians with type 2 diabetes [Pubmed: 31732824] |
| MOLECULAR AND CELLULAR BIOCHEMISTRY | Tumor suppressor genes are differentially regulated with dietary folate modulations in a rat model of hepatocellular carcinoma [Pubmed: 32974833] |
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