甘油醛-3-磷酸脫氫酶(GAPDH)單克隆抗體
Monoclonal Antibody to Glyceraldehyde-3-Phosphate Dehydrogenase (GAPDH)
G3PD; GAPD; Peptidyl-cysteine S-nitrosylase GAPDH
- 編號MAB932Hu22
- 物種Homo sapiens (Human，人) 相同的名稱，不同的物種。
- 來源單抗制備
- 宿主小鼠
- 效價(克隆號)3-2#
- Ig亞型 N/A
- 純化方式蛋白A + 蛋白G親和純化
- 標記物無標記物
- 免疫原 RPB932Hu01-甘油醛-3-磷酸脫氫酶(GAPDH)重組蛋白
- 緩沖液成份磷酸鹽緩沖液（pH7.4,含有0.02% NaN3和50%甘油）
- 性狀液體
- 濃度1mg/mL
- 且適物種Mus musculus (Mouse，小鼠), Rattus norvegicus (Rat，大鼠)
- 應用WB; IHC; ICC; IP.
如果抗體需用于流式細胞術，請參見流式抗體。 - 下載 英文說明書 中文說明書
- 規(guī)格 20μl100μl 200μl 1ml 10ml
- 價格 ¥ 752 ¥ 1756 ¥ 2508 ¥ 6270 ¥ 25080
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產品包裝(模擬)
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產品包裝(模擬)
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DAB staining on IHC-P; Sample: Human Liver Tissue Primary Ab: 30μg/ml Mouse Anti-Human GAPDH Antibody Control: Used PBS instead of primary antibody Second Ab: 2μg/ml HRP-Linked Caprine Anti-Mouse IgG Polyclonal Antibody (Catalog: SAA544Mu19)
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DAB staining on IHC-P;
Sample: Human Liver cancer Tissue
Primary Ab: 30μg/ml Mouse Anti-Human GAPDH Antibody
Control: Used PBS instead of primary antibody
Second Ab: 2μg/ml HRP-Linked Caprine Anti-Mouse IgG Polyclonal Antibody
(Catalog: SAA544Mu19) -
DAB staining on IHC-P;
Sample: Human Thyroid cancer Tissue
Primary Ab: 30μg/ml Mouse Anti-Human GAPDH Antibody
Control: Used PBS instead of primary antibody
Second Ab: 2μg/ml HRP-Linked Caprine Anti-Mouse IgG Polyclonal Antibody
(Catalog: SAA544Mu19)
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Figure. Western Blot; Sample: Recombinant GAPDH, Human.
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Western Blot; Samples: Lane1: Human Liver lysate; Lane2: Rat Liver lysate; Lane3: Mouse Liver lysate;
Primary Ab: 1μg/ml Mouse Anti-Human GAPDH Antibody
Second Ab: 0.2μg/ml HRP-Linked Caprine Anti-Mouse IgG Polyclonal Antibody
(Catalog: SAA544Mu19)
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通過ISO 9001、ISO 13485質量體系認證
特異性
該抗體是針對GAPDH的小鼠單克隆抗體。在免疫組織化學染色和免疫印跡實驗中能識別GAPDH。
用法
Western blotting: 0.01-5μg/mL;
Immunohistochemistry: 5-30μg/mL;
Immunocytochemistry: 5-20μg/mL;
Optimal working dilutions must be determined by end user.
儲存
經常使用則4°C保存。-20°C保存不超過兩年。避免反復凍融。
穩(wěn)定性
熱穩(wěn)定性以損失率顯示。損失率是由加速降解試驗決定，具體方法如下：在37°C孵育48小時，沒有顯著的降解或者沉淀產生。保質期內，在適當的條件下存儲，損失率低于5%。
贈品
增值服務
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| 編號 | 適用物種：Homo sapiens (Human，人) | 應用(僅供研究使用，不用于臨床診斷！) |
| RPB932Hu02 | 甘油醛-3-磷酸脫氫酶(GAPDH)重組蛋白 | Positive Control; Immunogen; SDS-PAGE; WB. |
| RPB932Hu03 | 甘油醛-3-磷酸脫氫酶(GAPDH)重組蛋白 | Positive Control; Immunogen; SDS-PAGE; WB. |
| RPB932Hu01 | 甘油醛-3-磷酸脫氫酶(GAPDH)重組蛋白 | Positive Control; Immunogen; SDS-PAGE; WB. |
| PAB932Hu01 | 甘油醛-3-磷酸脫氫酶(GAPDH)多克隆抗體 | WB; IHC; ICC; IP. |
| CAB932Hu01 | 抗甘油醛-3-磷酸脫氫酶(GAPDH)多克隆抗體 | Loading Control of WB. |
| PAB932Hu02 | 甘油醛-3-磷酸脫氫酶(GAPDH)多克隆抗體 | WB; IHC; ICC; IP; FCM |
| LAB932Hu71 | 甘油醛-3-磷酸脫氫酶(GAPDH)多克隆抗體(生物素標記) | WB; IHC; ICC. |
| MAB932Hu24 | 甘油醛-3-磷酸脫氫酶(GAPDH)單克隆抗體 | WB; IHC; ICC; IP. |
| MAB932Hu25 | 甘油醛-3-磷酸脫氫酶(GAPDH)單克隆抗體 | WB; IHC; ICC; IP. |
| MAB932Hu27 | 甘油醛-3-磷酸脫氫酶(GAPDH)單克隆抗體 | WB; IHC; ICC; IP. |
| MAB932Hu29 | 甘油醛-3-磷酸脫氫酶(GAPDH)單克隆抗體 | WB; IHC; ICC; IP. |
| MAB932Hu22 | 甘油醛-3-磷酸脫氫酶(GAPDH)單克隆抗體 | WB; IHC; ICC; IP. |
| MAB932Hu26 | 甘油醛-3-磷酸脫氫酶(GAPDH)單克隆抗體 | WB; IHC; ICC; IP. |
| MAB932Hu28 | 甘油醛-3-磷酸脫氫酶(GAPDH)單克隆抗體 | WB; IHC; ICC; IP. |
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| MAB932Hu21 | 甘油醛-3-磷酸脫氫酶(GAPDH)單克隆抗體 | WB; IHC; ICC; IP. |
| MAB932Hu23 | 甘油醛-3-磷酸脫氫酶(GAPDH)單克隆抗體 | WB; IHC; ICC; IP. |
| SEB932Hu | 甘油醛-3-磷酸脫氫酶(GAPDH)檢測試劑盒(酶聯(lián)免疫吸附試驗法) | Enzyme-linked immunosorbent assay for Antigen Detection. |
| SCB932Hu | 甘油醛-3-磷酸脫氫酶(GAPDH)檢測試劑盒(化學發(fā)光免疫分析法) | Chemiluminescent immunoassay for Antigen Detection. |
| LMB932Hu | 甘油醛-3-磷酸脫氫酶(GAPDH)等多因子檢測試劑盒(流式熒光發(fā)光法) | FLIA Kit for Antigen Detection. |
參考文獻
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| Clin Cancer Res | Inactivation of the p53–KLF4–CEBPA Axis in Acute Myeloid Leukemia [PubMed: 26408402] |
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| Diabetes & Metabolism | Dapagliflozin modulates glucagon secretion in an SGLT2-independent manner in murine alpha cells. [pubmed:28499695] |
| Scientific Reports | Adhesion of Toxoplasma gondii tachyzoite-infected vehicle leukocytes to capillary endothelial cells triggers timely parasite egression [pubmed:28720868] |
| Journal of Biological Chemistry | Biochemical characterization of the PHARC associated serine hydrolase ABHD12 reveals its preference for very long chain lipids [Pubmed: 30237167] |
| Biomaterials | Untangling the response of bone tumor cells and bone forming cells to matrix stiffness and adhesion ligand density by means of hydrogels [Pubmed: 30343256] |
| Bulletin of Experimental Biology and Medicine | Influence of Magnetite Nanoparticles and Quantum Dots on the Expression of Reference Genes in Peripheral Blood Cells [Pubmed: 30488202] |
| Journal?of?Medicinal?Chemistry | Discovery, Optimization and Target Identification of Novel Potent Broad-Spectrum Antiviral Inhibitors [Pubmed: 30938999] |
| International Immunopharmacology | Methionine enkephalin (MENK) regulates the immune pathogenesis of type 2 diabetes mellitus via the IL-33/ST2 pathway [Pubmed: 31078923] |
| Cancers | Rationale for a Combination Therapy Consisting of MCL1-and MEK-Inhibitors in Acute Myeloid Leukemia [Pubmed: 31718075] |
| Biological research | Linc-OIP5 in the breast cancer cells regulates angiogenesis of human umbilical vein endothelial cells through YAP1/Notch/NRP1 signaling circuit at a tumor … [Pubmed: 32046779] |
| Biomed Pharmacother | Silence of FAM83H-AS1 promotes chemosensitivity of gastric cancer through Wnt/β-catenin signaling pathway [Pubmed: 32028241] |
| Metab Brain Dis | Ginger and turmeric lipid-solubles attenuate heated oil-induced oxidative stress in the brain via the upregulation of NRF2 and improve cognitive function in rats [Pubmed: 33170419] |
| South African Journal of Botany | Evaluation of the effect of herbal extracts and their bioactive compounds against motion sickness by regulating neurotransmitter levels in vitro and in vivo [] |
| Regulation of hepatic lipid metabolism in CB1-/-and Abcb4-/--HBV surface protein transgenic mice [] | |
| Cancers (Basel) | BMI1-Inhibitor PTC596 in Combination with MCL1 Inhibitor S63845 or MEK Inhibitor Trametinib in the Treatment of Acute Leukemia. Cancers 2021, 13, 581 [33540760] |
| MOLECULAR CANCER RESEARCH | Omega-3 Eicosapentaenoic Acid Reduces Prostate Tumor Vascularity [33262291] |
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| Prog Neurobiol | Retinoic acid receptor beta protects striatopallidal medium spiny neurons from mitochondrial dysfunction and neurodegeneration [Pubmed:35151792] |
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