顆粒酶A(GZMA)多克隆抗體
Polyclonal Antibody to Granzyme A (GZMA)
CTLA3; HFSP; HF; H factor; Granzyme 1; Fragmentin-1; Hanukkah factor; Cytotoxic T-Lymphocyte-Associated Serine Esterase 3; CTL tryptase; Cytotoxic T-lymphocyte proteinase 1
- 編號PAA599Mu01
- 物種Mus musculus (Mouse，小鼠) 相同的名稱，不同的物種。
- 來源多抗制備
- 宿主兔
- 效價-
- Ig類型 IgG
- 純化方式抗原特異性親和純化
- 標記物無標記物
- 免疫原 RPA599Mu01-顆粒酶A(GZMA)重組蛋白
- 緩沖液成份0.01M 磷酸鹽緩沖液（pH7.4，containing 0.05% Proclin-300，50% glycerol）
- 性狀液體
- 濃度0.5mg/ml
- 且適物種Homo sapiens (Human，人), Rattus norvegicus (Rat，大鼠), Sus scrofa; Porcine (Pig，豬)
- 應(yīng)用WB
如果抗體需用于流式細胞術(shù)，請參見流式抗體。 - 下載 英文說明書 中文說明書
- 規(guī)格 20μl100μl 200μl 1ml 10ml
- 價格 ¥ 583 ¥ 1361 ¥ 1944 ¥ 4860 ¥ 19440
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產(chǎn)品包裝(模擬)
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產(chǎn)品包裝(模擬)
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IHC圖
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Western Blot; Sample: Rat Kidney lysate Primary Ab: 0.8μg/ml Rabbit Anti-Mouse GZMA Antibody Second Ab: 0.2μg/mL HRP-Linked Caprine Anti-Rabbit IgG Polyclonal Antibody (Catalog: SAA544Rb19)
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Western Blot; Sample: Lane1: Mouse Lung lysate; Lane2: Porcine Kidney lysate
Primary Ab: 1.5μg/ml Rabbit Anti-Mouse GZMA Antibody
Second Ab: 0.2μg/mL HRP-Linked Caprine Anti-Rabbit IgG Polyclonal Antibody
(Catalog: SAA544Rb19) -
Knockout Varification:
Lane 1: Wild-type Jurkat cell lysate;
Lane 2: GZMA knockout Jurkat cell lysate;
Predicted MW: 29kDa
Observed MW: 27kDa
Primary Ab: 5μg/ml Rabbit Anti-Mouse GZMA Antibody
Second Ab: 0.2μg/mL?HRP-Linked Caprine Anti-Rabbit IgG Polyclonal Antibody
(Catalog: SAA544Rb19) -
Western Blot; Samples: Lane1: Mouse Kidney lysate; Lane2: Porcine Kidney lysate; Lane3: Jurkat cell lysate;
Primary Ab: 2μg/ml Rabbit Anti-Mouse GZMA Antibody
Second Ab: 0.2μg/ml HRP-Linked Caprine Anti-Rabbit IgG Polyclonal Antibody
(Catalog: SAA544Rb19)
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通過ISO 9001、ISO 13485質(zhì)量體系認證
特異性
該抗體是針對GZMA的兔多克隆抗體。在免疫組織化學(xué)染色和免疫印跡實驗中能識別GZMA。
用法
Western blotting: 0.01-5μg/mL;
Immunohistochemistry: 5-20μg/mL;
Immunocytochemistry: 5-20μg/mL;
Optimal working dilutions must be determined by end user.
儲存
經(jīng)常使用則4°C保存。-20°C保存不超過兩年。避免反復(fù)凍融。
穩(wěn)定性
熱穩(wěn)定性以損失率顯示。損失率是由加速降解試驗決定，具體方法如下：在37°C孵育48小時，沒有顯著的降解或者沉淀產(chǎn)生。保質(zhì)期內(nèi)，在適當?shù)臈l件下存儲，損失率低于5%。
贈品
增值服務(wù)
相關(guān)產(chǎn)品
| 編號 | 適用物種：Mus musculus (Mouse，小鼠) | 應(yīng)用(僅供研究使用，不用于臨床診斷！) |
| RPA599Mu01 | 顆粒酶A(GZMA)重組蛋白 | Positive Control; Immunogen; SDS-PAGE; WB. |
| RPA599Mu02 | 顆粒酶A(GZMA)重組蛋白 | Positive Control; Immunogen; SDS-PAGE; WB. |
| PAA599Mu02 | 顆粒酶A(GZMA)多克隆抗體 | WB; IHC; ICC; IP. |
| PAA599Mu01 | 顆粒酶A(GZMA)多克隆抗體 | WB |
| LAA599Mu71 | 顆粒酶A(GZMA)多克隆抗體(生物素標記) | WB; IHC; ICC. |
| SEA599Mu | 顆粒酶A(GZMA)檢測試劑盒(酶聯(lián)免疫吸附試驗法) | Enzyme-linked immunosorbent assay for Antigen Detection. |
| LMA599Mu | 顆粒酶A(GZMA)等多因子檢測試劑盒(流式熒光發(fā)光法) | FLIA Kit for Antigen Detection. |
| KSA599Mu01 | 顆粒酶A(GZMA)檢測試劑盒DIY材料(酶聯(lián)免疫吸附試驗法) | 自己動手制作ELISA試劑盒的主要材料 |
參考文獻
| 雜志 | 參考文獻 |
| Cell Reports | Granzyme A Produces Bioactive IL-1β through a Nonapoptotic Inflammasome-Independent Pathway [Pubmed:25437548] |
| Journal of Dermatological Science | Relationships?among?plasma?granzyme?B?level,?pruritus?and?dermatitis?in?patients?with?atopicdermatitis. [pubmed:27686401] |
| Journal of Maternal-Fetal & Neonatal?Medicine | Extracellular granzyme A in amniotic fluid is elevated in the presence of sterile intra-amniotic inflammation in preterm prelabor rupture of membranes [Pubmed: 32912008] |
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