維生素D結(jié)合蛋白(DBP)多克隆抗體
Polyclonal Antibody to Vitamin D Binding Protein (DBP)
GC; VDBG; VDBP; Group-Specific Component; Gc-globulin
- 編號(hào)PAB810Hu01
- 物種Homo sapiens (Human，人) 相同的名稱，不同的物種。
- 來源多抗制備
- 宿主兔
- 效價(jià)-
- Ig類型 IgG
- 純化方式抗原特異性親和純化
- 標(biāo)記物無標(biāo)記物
- 免疫原 RPB810Hu01-維生素D結(jié)合蛋白(DBP)重組蛋白
- 緩沖液成份磷酸鹽緩沖液（pH7.4,含有0.02% NaN3和50%甘油）
- 性狀液體
- 濃度0.5mg/ml
- 且適物種Mus musculus (Mouse，小鼠), Sus scrofa; Porcine (Pig，豬)
- 應(yīng)用WB; IHC; ICC; IP; FCM
如果抗體需用于流式細(xì)胞術(shù)，請(qǐng)參見流式抗體。 - 下載 英文說明書 中文說明書
- 規(guī)格 20μl100μl 200μl 1ml 10ml
- 價(jià)格 ¥ 598 ¥ 1394 ¥ 1992 ¥ 4980 ¥ 19920
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產(chǎn)品包裝(模擬)
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產(chǎn)品包裝(模擬)
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DAB staining on IHC-P; Samples: Human Kidney Tissue; Primary Ab: 20μg/ml Rabbit Anti-Human DBP Antibody Second Ab: 2μg/mL?HRP-Linked Caprine Anti-Rabbit IgG Polyclonal Antibody (Catalog: SAA544Rb19)
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DAB staining on IHC-P;
Samples: Human Placenta Tissue;
Primary Ab: 20μg/ml Rabbit Anti-Human DBP Antibody
Second Ab: 2μg/mL?HRP-Linked Caprine Anti-Rabbit IgG Polyclonal Antibody
(Catalog: SAA544Rb19) -
DAB staining on IHC-P;
Sample: Human Placenta Tissue
Primary Ab: 20μg/ml Rabbit Anti-Human DBP Antibody
Control: Used PBS instead of primary antibody
Second Ab: 2μg/ml HRP-Linked Caprine Anti-Rabbit IgG Polyclonal Antibody
(Catalog: SAA544Rb19) -
DAB staining on IHC-P;
Sample: Human Kidney Tissue
Primary Ab: 20μg/ml Rabbit Anti-Human DBP Antibody
Control: Used PBS instead of primary antibody
Second Ab: 2μg/ml HRP-Linked Caprine Anti-Rabbit IgG Polyclonal Antibody
(Catalog: SAA544Rb19)
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Figure. Western Blot; Sample: Recombinant DBP, Human.
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Western Blot; Sample: Mouse Liver lysate;
Primary Ab: 1μg/ml Rabbit Anti-Human DBP Antibody
Second Ab: 0.2μg/mL?HRP-Linked Caprine Anti-Rabbit IgG Polyclonal Antibody
(Catalog: SAA544Rb19) -
Western Blot; Sample: Human Serum;
Primary Ab: 1μg/ml Rabbit Anti-Human DBP Antibody
Second Ab: 0.2μg/mL?HRP-Linked Caprine Anti-Rabbit IgG Polyclonal Antibody
(Catalog: SAA544Rb19) -
Western Blot; Samples: Lane1: Mouse Liver lysate; Lane2: LO2 cell lysate; Lane3: Human Lung lysate;
Primary Ab: 0.1μg/ml Rabbit Anti-Human DBP Antibody
Second Ab: 0.2μg/ml HRP-Linked Caprine Anti-Rabbit IgG Polyclonal Antibody
(Catalog: SAA544Rb19) -
Human NB-4 cell line was fixed with 2% paraformaldehyde (10 min) , permeabilised with 0.1% BSA-Triton X-100,then stained with 20μg/ml rabbit Anti-human DBP Polyclonal Antibody (Catalog PAB810Hu01, red histogram) or Isotype control antibody (Catalog IS067-Rb01, green histogram), followed by 1μg/ml FITC-conjugated Anti-rabbit IgG Secondary Antibody (Catalog SAA544Rb18).
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通過ISO 9001、ISO 13485質(zhì)量體系認(rèn)證
特異性
該抗體是針對(duì)DBP的兔多克隆抗體。在免疫組織化學(xué)染色和免疫印跡實(shí)驗(yàn)中能識(shí)別DBP。
用法
Western blotting: 0.01-2μg/mL;
Immunohistochemistry: 5-20μg/mL;
Immunocytochemistry: 5-20μg/mL;
Flow cytometry:20μg/ml;
Optimal working dilutions must be determined by end user.
儲(chǔ)存
經(jīng)常使用則4°C保存。-20°C保存不超過兩年。避免反復(fù)凍融。
穩(wěn)定性
熱穩(wěn)定性以損失率顯示。損失率是由加速降解試驗(yàn)決定，具體方法如下：在37°C孵育48小時(shí)，沒有顯著的降解或者沉淀產(chǎn)生。保質(zhì)期內(nèi)，在適當(dāng)?shù)臈l件下存儲(chǔ)，損失率低于5%。
贈(zèng)品
增值服務(wù)
抗體標(biāo)記定制服務(wù)
Protein-A/G純化柱
細(xì)胞和組織染色液
抗體陽(yáng)性對(duì)照品
組織/切片定制服務(wù)
磷酸化抗體定制服務(wù)
免疫印跡(WB)實(shí)驗(yàn)服務(wù)
免疫組織化學(xué)(IHC)實(shí)驗(yàn)服務(wù)
免疫細(xì)胞化學(xué)(ICC)實(shí)驗(yàn)服務(wù)
流式細(xì)胞術(shù)(FCM)實(shí)驗(yàn)服務(wù)
免疫沉淀(IP)實(shí)驗(yàn)服務(wù)
免疫熒光(IF)實(shí)驗(yàn)服務(wù)
緩沖液
DAB顯色試劑盒
SABC試劑盒
長(zhǎng)臂生物素標(biāo)記試劑盒
實(shí)時(shí)熒光定量PCR實(shí)驗(yàn)服務(wù)
相關(guān)產(chǎn)品
| 編號(hào) | 適用物種：Homo sapiens (Human，人) | 應(yīng)用(僅供研究使用，不用于臨床診斷！) |
| RPB810Hu02 | 維生素D結(jié)合蛋白(DBP)重組蛋白 | Positive Control; Immunogen; SDS-PAGE; WB. |
| APB810Hu61 | 維生素D結(jié)合蛋白(DBP)活性蛋白 | Cell?culture;?Activity?Assays. |
| EPB810Hu61 | 維生素D結(jié)合蛋白(DBP)真核蛋白 | Positive Control; Immunogen; SDS-PAGE; WB. |
| RPB810Hu03 | 維生素D結(jié)合蛋白(DBP)重組蛋白 | Positive Control; Immunogen; SDS-PAGE; WB. |
| RPB810Hu01 | 維生素D結(jié)合蛋白(DBP)重組蛋白 | Positive Control; Immunogen; SDS-PAGE; WB. |
| PAB810Hu03 | 維生素D結(jié)合蛋白(DBP)多克隆抗體 | WB; IHC; ICC; IP. |
| PAB810Hu02 | 維生素D結(jié)合蛋白(DBP)多克隆抗體 | WB; IHC; ICC; IP. |
| PAB810Hu01 | 維生素D結(jié)合蛋白(DBP)多克隆抗體 | WB; IHC; ICC; IP; FCM |
| LAB810Hu71 | 維生素D結(jié)合蛋白(DBP)多克隆抗體(生物素標(biāo)記) | WB; IHC; ICC. |
| MAB810Hu26 | 維生素D結(jié)合蛋白(DBP)單克隆抗體 | WB; IHC; ICC; IP. |
| MAB810Hu22 | 維生素D結(jié)合蛋白(DBP)單克隆抗體 | WB,IHC |
| MAB810Hu24 | 維生素D結(jié)合蛋白(DBP)單克隆抗體 | WB; IHC; ICC; IP. |
| MAB810Hu25 | 維生素D結(jié)合蛋白(DBP)單克隆抗體 | WB; IHC; ICC; IP. |
| MAB810Hu27 | 維生素D結(jié)合蛋白(DBP)單克隆抗體 | WB. |
| MAB810Hu21 | 維生素D結(jié)合蛋白(DBP)單克隆抗體 | WB; IHC; ICC; IP. |
| MAB810Hu23 | 維生素D結(jié)合蛋白(DBP)單克隆抗體 | WB; IHC; ICC; IP. |
| FAB810Hu04 | 抗維生素D結(jié)合蛋白(DBP)單克隆抗體 | FCM |
| FAB810Hu02 | 抗維生素D結(jié)合蛋白(DBP)單克隆抗體 | FCM |
| SEB810Hu | 維生素D結(jié)合蛋白(DBP)檢測(cè)試劑盒(酶聯(lián)免疫吸附試驗(yàn)法) | Enzyme-linked immunosorbent assay for Antigen Detection. |
| AEB810Hu | 抗維生素D結(jié)合蛋白抗體(Anti-DBP)檢測(cè)試劑盒(酶聯(lián)免疫吸附試驗(yàn)法) | Enzyme-linked immunosorbent assay for Antibody Detection. |
| SCB810Hu | 維生素D結(jié)合蛋白(DBP)檢測(cè)試劑盒(化學(xué)發(fā)光免疫分析法) | Chemiluminescent immunoassay for Antigen Detection. |
| LMB810Hu | 維生素D結(jié)合蛋白(DBP)等多因子檢測(cè)試劑盒(流式熒光發(fā)光法) | FLIA Kit for Antigen Detection. |
| KSB810Hu01 | 維生素D結(jié)合蛋白(DBP)檢測(cè)試劑盒DIY材料(酶聯(lián)免疫吸附試驗(yàn)法) | Main materials for "Do It (ELISA Kit) Yourself". |
參考文獻(xiàn)
| 雜志 | 參考文獻(xiàn) |
| Proteome Science | Isotope Coded Protein Labeling analysis of plasma specimens from acute severe dengue fever patients [PubMed: 23101585] |
| Toxins (Basel). | Increased Circulating Levels of Vitamin D Binding Protein in MS Patients [Pubmed:25590278] |
| Endocrine | Vitamin D-binding protein and free vitamin D concentrations in acromegaly [PubMed: 26547217] |
| Proteomics Clinical Applications | Comparative mass spectrometric and immunoassay‐based proteome analysis in serum of Duchenne muscular dystrophy patients [Pubmed:26680509] |
| PLoS One. | 25-Hydroxivitamin D Serum Concentration, Not Free and Bioavailable Vitamin D, Is Associated with Disease Activity in Systemic Lupus Erythematosus Patients. [pubmed:28085957] |
| PROTEOMICS?-?Clinical?Applications | Comparative mass spectrometric and immunoassay-based?proteome?analysis?in?serum?ofDuchenne muscular dystrophy?patients. [pubmed:26680509] |
| Plos One | 25-Hydroxivitamin D serum concentration, not free and bioavailable vitamin D, is associated with disease activity in systemic lupus erythematosus patients [10.1371/journal.pone.0170323] |
| Nutrients | Gene Expression of Sirtuin-1 and Endogenous Secretory Receptor for Advanced Glycation End Products in Healthy and Slightly Overweight Subjects after?… [Pubmed:30037068] |
| BBA- Molecular Basis of Disease | Enhanced remedial effects for vitamin D3 and calcium co-supplementation against pre-existing lead nephrotoxicity in mice: The roles of renal calcium homeostatic?… [Pubmed: 30553018] |
| J Clin Res Pediatr Endocrinol | Is Bioavailable Vitamin D Better Than Total Vitamin D to Evaluate Vitamin D Status in Obese Children? [34013709] |
| PLoS One | Leukocyte telomere length as a compensatory mechanism in vitamin D metabolism [Pubmed:35202418] |
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