親核素α2(KPNa2)多克隆抗體
Polyclonal Antibody to Karyopherin Alpha 2 (KPNa2)
IPOA1; QIP2; RCH1; SRP1alpha; RAG Cohort 1; Importin Alpha 1; RAG cohort protein 1; SRP1-alpha
- 編號PAE748Hu01
- 物種Homo sapiens (Human，人) 相同的名稱，不同的物種。
- 來源多抗制備
- 宿主兔
- 效價-
- Ig類型 IgG
- 純化方式抗原特異性親和純化
- 標記物無標記物
- 免疫原 RPE748Hu01-親核素α2(KPNa2)重組蛋白
- 緩沖液成份0.01M 磷酸鹽緩沖液（pH7.4，containing 0.05% Proclin-300，50% glycerol）
- 性狀液體
- 濃度0.5mg/mL
- 且適物種-
- 應用WB,IHC,ICC/IF; FCM
如果抗體需用于流式細胞術，請參見流式抗體。 - 下載 英文說明書 中文說明書
- 規(guī)格 20μl100μl 200μl 1ml 10ml
- 價格 ¥ 630 ¥ 1470 ¥ 2100 ¥ 5250 ¥ 21000
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產(chǎn)品包裝(模擬)
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產(chǎn)品包裝(模擬)
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FITC staining on IF; Samples: Human HepG2 cell; Primary Ab: 20μg/ml Rabbit Anti-Human KPNa2 Antibody Second Ab: 1.5μg/ml FITC-Linked Caprine Anti-Rabbit IgG Polyclonal Antibody (Catalog: SAA544Rb18)
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DAB staining on IHC-P;
Sample: Rat Lymph node Tissue;
Primary Ab: 20ug/ml Rabbit Anti-Human KPNa2 Antibody
Second Ab: 2μg/mL?HRP-Linked Caprine Anti-Rabbit IgG Polyclonal Antibody
(Catalog: SAA544Rb19) -
DAB staining on IHC-P;
Sample: Rat Testis Tissue;
Primary Ab: 20ug/ml Rabbit Anti-Human KPNa2 Antibody
Second Ab: 2μg/mL?HRP-Linked Caprine Anti-Rabbit IgG Polyclonal Antibody
(Catalog: SAA544Rb19) -
AF488 staining on IF;<br/>Sample: HepG2 cell<br/>Primary Ab: 20μg/ml Rabbit Anti-Human KPNa2 Antibody<br/>Second Ab: 2μg/ml AF488-Linked Caprine Anti-Rabbit IgG Polyclonal Antibody<br/>(Catalog: SAA544Rb11)
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Figure. Western Blot; Sample: Recombinant KPNa2, Human.
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Western Blot; Sample: Lane1: 293T cell lysate; Lane2: Hela cell lysate; Lane3: Raji cell lysate; Lane4: U2OS cell lysate
Primary Ab: 0.2μg/ml Rabbit Anti-Human KPNa2 Antibody
Second Ab: 0.2μg/mL HRP-Linked Caprine Anti-Rabbit IgG Polyclonal Antibody
(Catalog: SAA544Rb19) -
Knockout Varification: <br/>Lane 1: Wild-type HepG2 cell lysate; <br/>Lane 2: KPNa2 knockout HepG2 cell lysate; <br/>Predicted MW: 58kDa <br/>Observed MW: 58kDa<br/>Primary Ab: 3μg/ml Rabbit Anti-Human KPNa2 Antibody<br/>Second Ab: 0.2μg/mL?HRP-Linked Caprine Anti-Rabbit IgG Polyclonal Antibody<br/>(Catalog: SAA544Rb19)
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Human A549 cell was fixed with 2% paraformaldehyde (10 min) , permeabilised with 0.1% BSA-Triton X-100,then stained with 20μg/ml rabbit Anti-human KPNa2 Polyclonal Antibody (Catalog PAE748Hu01, red histogram) or Isotype control antibody (Catalog IS067-Rb01, green histogram), followed by 1μg/ml FITC-conjugated Anti-rabbit IgG Secondary Antibody (Catalog SAA544Rb18).
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通過ISO 9001、ISO 13485質量體系認證
特異性
該抗體是針對KPNa2的兔多克隆抗體。在免疫組織化學染色和免疫印跡實驗中能識別KPNa2。
用法
Western blotting: 0.01-2μg/mL;
Immunohistochemistry: 5-20μg/mL;
Immunofluorescence:5-20μg/mL;
Flow cytometry:20μg/mL;
Optimal working dilutions must be determined by end user.
儲存
經(jīng)常使用則4°C保存。-20°C保存不超過兩年。避免反復凍融。
穩(wěn)定性
熱穩(wěn)定性以損失率顯示。損失率是由加速降解試驗決定，具體方法如下：在37°C孵育48小時，沒有顯著的降解或者沉淀產(chǎn)生。保質期內，在適當?shù)臈l件下存儲，損失率低于5%。
贈品
增值服務
相關產(chǎn)品
| 編號 | 適用物種：Homo sapiens (Human，人) | 應用(僅供研究使用，不用于臨床診斷！) |
| RPE748Hu01 | 親核素α2(KPNa2)重組蛋白 | Positive Control; Immunogen; SDS-PAGE; WB. |
| PAE748Hu01 | 親核素α2(KPNa2)多克隆抗體 | WB,IHC,ICC/IF; FCM |
| MAE748Hu21 | 親核素α2(KPNa2)單克隆抗體 | WB; ICC/IF;FCM |
| MAE748Hu22 | 親核素α2(KPNa2)單克隆抗體 | WB; IHC; ICC; IP. |
| MAE748Hu23 | 親核素α2(KPNa2)單克隆抗體 | WB; IHC |
| MAE748Hu24 | 親核素α2(KPNa2)單克隆抗體 | WB; IHC; ICC; IP. |
| MAE748Hu25 | 親核素α2(KPNa2)單克隆抗體 | WB; IHC |
| MAE748Hu26 | 親核素α2(KPNa2)單克隆抗體 | WB; IHC; ICC; IP. |
| FAE748Hu08 | 抗親核素α2(KPNa2)單克隆抗體 | Flow cytometry. |
| FAE748Hu06 | 抗親核素α2(KPNa2)單克隆抗體 | Flow cytometry. |
| FAE748Hu04 | 抗親核素α2(KPNa2)單克隆抗體 | Flow cytometry. |
| FAE748Hu02 | 抗親核素α2(KPNa2)單克隆抗體 | Flow cytometry. |
| SEE748Hu | 親核素α2(KPNa2)檢測試劑盒(酶聯(lián)免疫吸附試驗法) | Enzyme-linked immunosorbent assay for Antigen Detection. |
| LME748Hu | 親核素α2(KPNa2)等多因子檢測試劑盒(流式熒光發(fā)光法) | FLIA Kit for Antigen Detection. |
| KSE748Hu01 | 親核素α2(KPNa2)檢測試劑盒DIY材料(酶聯(lián)免疫吸附試驗法) | Main materials for "Do It(ELISA Kit) Yourself" |
參考文獻
| 雜志 | 參考文獻 |
| Oncol Rep.? | KPNA2 is a promising biomarker candidate for esophageal squamous cell carcinoma and correlates with cell proliferation [Pubmed:25109899] |
| Tumor Biology | KPNA2 is a potential diagnostic serum biomarker for epithelial ovarian cancer and correlates with poor prognosis [pubmed:28651492] |
| Head?&?Neck | Association of overexpressed karyopherin alpha 2 with poor survival and its contribution to interleukin‐1β‐induced matrix metalloproteinase expression in oral cancer [Pubmed:29542209] |
| International Journal of Cancer | Nuclear transport proteins are secreted by cancer cells and identified as potential novel cancer biomarkers [34591985] |
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