角蛋白8(CK8)多克隆抗體
Polyclonal Antibody to Cytokeratin 8 (CK8)
K8; CARD2; KRT8; CYK8; K2C8; KO; Keratin 8; Keratin, type II cytoskeletal 8
- 編號(hào)PAC025Hu01
- 物種Homo sapiens (Human，人) 相同的名稱，不同的物種。
- 來源多抗制備
- 宿主兔
- 效價(jià)-
- Ig類型 IgG
- 純化方式抗原特異性親和純化
- 標(biāo)記物無標(biāo)記物
- 免疫原 RPC025Hu01-角蛋白8(CK8)重組蛋白
- 緩沖液成份0.01M 磷酸鹽緩沖液（pH7.4，containing 0.05% Proclin-300，50% glycerol）
- 性狀液體
- 濃度0.5mg/ml
- 且適物種-
- 應(yīng)用WB,IHC,ICC/IF; FCM
如果抗體需用于流式細(xì)胞術(shù)，請參見流式抗體。 - 下載 英文說明書 中文說明書
- 規(guī)格 20μl100μl 200μl 1ml 10ml
- 價(jià)格 ¥ 630 ¥ 1470 ¥ 2100 ¥ 5250 ¥ 21000
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產(chǎn)品包裝(模擬)
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產(chǎn)品包裝(模擬)
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DAB staining on IHC-P; Samples: Human Liver cancer Tissue; Primary Ab: 20μg/ml Rabbit Anti-Human CK8 Antibody Second Ab: 2μg/mL?HRP-Linked Caprine Anti-Rabbit IgG Polyclonal Antibody (Catalog: SAA544Rb19)
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DAB staining on IHC-P;
Samples: Human Tonsil Tissue;
Primary Ab: 10μg/ml Rabbit Anti-Human KRT8 Antibody
Second Ab: 2μg/mL?HRP-Linked Caprine Anti-Rabbit IgG Polyclonal Antibody
(Catalog: SAA544Rb19) -
DAB staining on IHC-P;
Samples: Human Prostate Tissue;
Primary Ab: 10μg/ml Rabbit Anti-Human KRT8 Antibody
Second Ab: 2μg/mL?HRP-Linked Caprine Anti-Rabbit IgG Polyclonal Antibody
(Catalog: SAA544Rb19) -
DAB staining on IHC-P;
Samples: Human Thyroid cancer Tissue;
Primary Ab: 10μg/ml Rabbit Anti-Human KRT8 Antibody
Second Ab: 2μg/mL?HRP-Linked Caprine Anti-Rabbit IgG Polyclonal Antibody
(Catalog: SAA544Rb19) -
DAB staining on IHC-P;
Samples: Human Kidney Tissue;
Primary Ab: 10μg/ml Rabbit Anti-Human KRT8 Antibody
Second Ab: 2μg/mL?HRP-Linked Caprine Anti-Rabbit IgG Polyclonal Antibody
(Catalog: SAA544Rb19) -
DAB staining on IHC-P;
Samples: Human Stomach Tissue;
Primary Ab: 10μg/ml Rabbit Anti-Human KRT8 Antibody
Second Ab: 2μg/mL?HRP-Linked Caprine Anti-Rabbit IgG Polyclonal Antibody
(Catalog: SAA544Rb19) -
FITC staining on IF;
Samples: Human MCF7 cell;
Primary Ab: 20μg/ml Rabbit Anti-Human KRT8 Antibody
Second Ab: 1.5μg/ml FITC-Linked Caprine Anti-Rabbit IgG Polyclonal Antibody
(Catalog: SAA544Rb18)
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Figure. Western Blot; Sample: Recombinant CK8, Human.
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Knockout Varification: <br/>Lane 1: Wild-type Hela cell lysate; <br/>Lane 2: KRT8 knockout Hela cell lysate; <br/>Predicted MW: 54,57kDa <br/>Observed MW: 57kDa<br/>Primary Ab: 2μg/ml Rabbit Anti-Human KRT8 Antibody<br/>Second Ab: 0.2μg/mL?HRP-Linked Caprine Anti-Rabbit IgG Polyclonal Antibody<br/>(Catalog: SAA544Rb19)
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Western Blot; Sample: Lane1: A431 cell lysate; Lane2: A549 cell lysate; Lane3: Hela cell lysate; Lane4: MCF7 cell lysate
Primary Ab: 0.05μg/ml Rabbit Anti-Human CK8 Antibody
Second Ab: 0.2μg/mL HRP-Linked Caprine Anti-Rabbit IgG Polyclonal Antibody
(Catalog: SAA544Rb19) -
Human MCF7 cell was fixed with 2% paraformaldehyde (10 min) , permeabilised with 0.2% BSA-Triton X-100,then stained with 20μg/ml rabbit Anti-human CK8 Polyclonal Antibody (Catalog PAC025Hu01, red histogram) or Isotype control antibody (Catalog IS067-Rb01, green histogram), followed by 1μg/ml FITC-conjugated Anti-rabbit IgG Secondary Antibody (Catalog SAA544Rb18).
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通過ISO 9001、ISO 13485質(zhì)量體系認(rèn)證
特異性
該抗體是針對CK8的兔多克隆抗體。在免疫組織化學(xué)染色和免疫印跡實(shí)驗(yàn)中能識(shí)別CK8。
用法
Western blotting: 0.01-2μg/mL;
Immunohistochemistry: 5-20μg/mL;
Immunocytochemistry: 5-20μg/mL;
Flow cytometry:20μg/ml;
Optimal working dilutions must be determined by end user.
儲(chǔ)存
經(jīng)常使用則4°C保存。-20°C保存不超過兩年。避免反復(fù)凍融。
穩(wěn)定性
熱穩(wěn)定性以損失率顯示。損失率是由加速降解試驗(yàn)決定，具體方法如下：在37°C孵育48小時(shí)，沒有顯著的降解或者沉淀產(chǎn)生。保質(zhì)期內(nèi)，在適當(dāng)?shù)臈l件下存儲(chǔ)，損失率低于5%。
贈(zèng)品
增值服務(wù)
抗體標(biāo)記定制服務(wù)
Protein-A/G純化柱
細(xì)胞和組織染色液
抗體陽性對照品
組織/切片定制服務(wù)
磷酸化抗體定制服務(wù)
免疫印跡(WB)實(shí)驗(yàn)服務(wù)
免疫組織化學(xué)(IHC)實(shí)驗(yàn)服務(wù)
免疫細(xì)胞化學(xué)(ICC)實(shí)驗(yàn)服務(wù)
流式細(xì)胞術(shù)(FCM)實(shí)驗(yàn)服務(wù)
免疫沉淀(IP)實(shí)驗(yàn)服務(wù)
免疫熒光(IF)實(shí)驗(yàn)服務(wù)
緩沖液
DAB顯色試劑盒
SABC試劑盒
長臂生物素標(biāo)記試劑盒
實(shí)時(shí)熒光定量PCR實(shí)驗(yàn)服務(wù)
相關(guān)產(chǎn)品
| 編號(hào) | 適用物種：Homo sapiens (Human，人) | 應(yīng)用(僅供研究使用，不用于臨床診斷！) |
| RPC025Hu01 | 角蛋白8(CK8)重組蛋白 | Positive Control; Immunogen; SDS-PAGE; WB. |
| RPC025Hu02 | 角蛋白8(CK8)重組蛋白 | Positive Control; Immunogen; SDS-PAGE; WB. |
| PAC025Hu02 | 角蛋白8(CK8)多克隆抗體 | WB; IHC; ICC; IP. |
| PAC025Hu01 | 角蛋白8(CK8)多克隆抗體 | WB,IHC,ICC/IF; FCM |
| LAC025Hu81 | 角蛋白8(CK8)多克隆抗體(異硫氰酸熒光素標(biāo)記) | WB; IHC; ICC; IF. |
| LAC025Hu71 | 角蛋白8(CK8)多克隆抗體(生物素標(biāo)記) | WB; IHC; ICC. |
| MAC025Hu21 | 角蛋白8(CK8)單克隆抗體 | WB; IHC; ICC; IP. |
| MAC025Hu23 | 角蛋白8(CK8)單克隆抗體 | WB; IHC; ICC; IP. |
| MAC025Hu22 | 角蛋白8(CK8)單克隆抗體 | WB; IHC; ICC; IP. |
| SEC025Hu | 角蛋白8(CK8)檢測試劑盒(酶聯(lián)免疫吸附試驗(yàn)法) | Enzyme-linked immunosorbent assay for Antigen Detection. |
| LMC025Hu | 角蛋白8(CK8)等多因子檢測試劑盒(流式熒光發(fā)光法) | FLIA Kit for Antigen Detection. |
參考文獻(xiàn)
| 雜志 | 參考文獻(xiàn) |
| Molecular?Medicine?Reports | Placental protein 14 as a potential biomarker for diagnosis of preterm premature rupture of membranes [Pubmed:29749501] |
