中期因子(MK)單克隆抗體
Monoclonal Antibody to Midkine (MK)
MDK; ARAP; NEGF2; Neurite Growth-Promoting Factor 2; Amphiregulin-associated protein; Midgestation and kidney protein; Neurite outgrowth-promoting factor 2
- 編號MAA631Hu22
- 物種Homo sapiens (Human,人) 相同的名稱,不同的物種。
- 來源單抗制備
- 宿主小鼠
- 效價(jià)(克隆號)C1
- Ig亞型 IgG1 Kappa
- 純化方式蛋白A + 蛋白G親和純化
- 標(biāo)記物無標(biāo)記物
- 免疫原 RPA631Hu01-中期因子(MK)重組蛋白
- 緩沖液成份0.01M 磷酸鹽緩沖液(pH7.4,containing 0.05% Proclin-300,50% glycerol)
- 性狀液體
- 濃度0.77mg/ml
- 且適物種Mus musculus (Mouse,小鼠), Rattus norvegicus (Rat,大鼠)
- 應(yīng)用WB; IHC; ICC; IP.
如果抗體需用于流式細(xì)胞術(shù),請參見流式抗體。 - 下載 英文說明書 中文說明書
- 規(guī)格 20μl100μl 200μl 1ml 10ml
- 價(jià)格 ¥ 605 ¥ 1411 ¥ 2016 ¥ 5040 ¥ 20160
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特異性
該抗體是針對MK的小鼠單克隆抗體。在免疫組織化學(xué)染色和免疫印跡實(shí)驗(yàn)中能識別MK。
用法
Western blotting: 0.5-5μg/mL
Immunohistochemistry: 5-30μg/mL
Immunocytochemistry: 5-30μg/mL
Optimal working dilutions must be determined by end user.
儲存
經(jīng)常使用則4°C保存。-20°C保存不超過兩年。避免反復(fù)凍融。
穩(wěn)定性
熱穩(wěn)定性以損失率顯示。損失率是由加速降解試驗(yàn)決定,具體方法如下:在37°C孵育48小時(shí),沒有顯著的降解或者沉淀產(chǎn)生。保質(zhì)期內(nèi),在適當(dāng)?shù)臈l件下存儲,損失率低于5%。
贈品
增值服務(wù)
抗體標(biāo)記定制服務(wù)
Protein-A/G純化柱
細(xì)胞和組織染色液
抗體陽性對照品
組織/切片定制服務(wù)
磷酸化抗體定制服務(wù)
免疫印跡(WB)實(shí)驗(yàn)服務(wù)
免疫組織化學(xué)(IHC)實(shí)驗(yàn)服務(wù)
免疫細(xì)胞化學(xué)(ICC)實(shí)驗(yàn)服務(wù)
流式細(xì)胞術(shù)(FCM)實(shí)驗(yàn)服務(wù)
免疫沉淀(IP)實(shí)驗(yàn)服務(wù)
免疫熒光(IF)實(shí)驗(yàn)服務(wù)
緩沖液
DAB顯色試劑盒
SABC試劑盒
長臂生物素標(biāo)記試劑盒
實(shí)時(shí)熒光定量PCR實(shí)驗(yàn)服務(wù)
相關(guān)產(chǎn)品
編號 | 適用物種:Homo sapiens (Human,人) | 應(yīng)用(僅供研究使用,不用于臨床診斷!) |
RPA631Hu02 | 中期因子(MK)重組蛋白 | Positive Control; Immunogen; SDS-PAGE; WB. |
RPA631Hu01 | 中期因子(MK)重組蛋白 | Positive Control; Immunogen; SDS-PAGE; WB. |
APA631Hu01 | 中期因子(MK)活性蛋白 | Cell?culture;?Activity?Assays. |
PAA631Hu02 | 中期因子(MK)多克隆抗體 | WB; IHC; ICC; IP. |
PAA631Hu01 | 中期因子(MK)多克隆抗體 | WB; IHC; ICC; IP. |
LAA631Hu71 | 中期因子(MK)多克隆抗體(生物素標(biāo)記) | WB; IHC; ICC. |
LAA631Hu81 | 中期因子(MK)多克隆抗體(異硫氰酸熒光素標(biāo)記) | WB; IHC; ICC; IF. |
MAA631Hu22 | 中期因子(MK)單克隆抗體 | WB; IHC; ICC; IP. |
MAA631Hu21 | 中期因子(MK)單克隆抗體 | WB; IHC; ICC; IP. |
SEA631Hu | 中期因子(MK)檢測試劑盒(酶聯(lián)免疫吸附試驗(yàn)法) | Enzyme-linked immunosorbent assay for Antigen Detection. |
LMA631Hu | 中期因子(MK)等多因子檢測試劑盒(流式熒光發(fā)光法) | FLIA Kit for Antigen Detection. |
KSA631Hu01 | 中期因子(MK)檢測試劑盒DIY材料(酶聯(lián)免疫吸附試驗(yàn)法) | Main materials for "Do It (ELISA Kit) Yourself". |
參考文獻(xiàn)
雜志 | 參考文獻(xiàn) |
Midkine: From Embryogenesis to Pathogenesis and Therapy | Immunochemical Methods for the Detection of Midkine in Samples of Human Origin [Springer: Source] |
33 | Midkine Controls Arteriogenesis by Regulating the Bioavailability of Vascular Endothelial Growth Factor A and the Expression of Nitric Oxide Synthase 1 and … [10.1016:j.ebiom.2017.11.020] |
Circulation Journal | Midkine Promotes Atherosclerotic Plaque Formation Through Its Pro-Inflammatory, Angiogenic and Anti-Apoptotic Functions in Apolipoprotein E-Knockout Mice [pubmed:28781288] |
EBioMedicine | Midkine Controls Arteriogenesis by Regulating the Bioavailability of Vascular Endothelial Growth Factor A and the Expression of Nitric Oxide Synthase 1 and?… [Pubmed:29233575] |
Journal of Clinical Laboratory Analysis | Clinical value of jointly detection pleural fluid Midkine, pleural fluid adenosine deaminase, and pleural fluid carbohydrate antigen 125 in the identification of nonsmall?… [Pubmed:29797475] |
Charakterisierung der funktionalen Rolle des Zytokins Midkine bei der Arteriogenese [:] | |
Scientific Reports | The possible role of Dickkopf-1, Golgi protein-73 and Midkine as predictors of hepatocarcinogenesis: a review and an Egyptian study [Pubmed: 32198440] |
Odontology | Effects of different pulp-capping materials on cell death signaling pathways of lipoteichoic acid-stimulated human dental pulp stem cells [Pubmed: 33206337] |