O-6-甲基鳥嘌呤DNA甲基轉(zhuǎn)移酶(MGMT)多克隆抗體
Polyclonal Antibody to O-6-Methylguanine DNA Methyltransferase (MGMT)
Methylated-DNA--Protein-Cysteine Methyltransferase; 6-O-methylguanine-DNA methyltransferase; Methylated-DNA--protein-cysteine methyltransferase
- 編號PAC027Hu01
- 物種Homo sapiens (Human，人) 相同的名稱，不同的物種。
- 來源多抗制備
- 宿主兔
- 效價-
- Ig類型 IgG
- 純化方式抗原特異性親和純化
- 標(biāo)記物無標(biāo)記物
- 免疫原 RPC027Hu01-O-6-甲基鳥嘌呤DNA甲基轉(zhuǎn)移酶(MGMT)重組蛋白
- 緩沖液成份0.01M 磷酸鹽緩沖液（pH7.4，containing 0.05% Proclin-300，50% glycerol）
- 性狀液體
- 濃度0.5mg/ml
- 且適物種Sus scrofa; Porcine (Pig，豬)
- 應(yīng)用WB,IHC
如果抗體需用于流式細(xì)胞術(shù)，請參見流式抗體。 - 下載 英文說明書 中文說明書
- 規(guī)格 20μl100μl 200μl 1ml 10ml
- 價格 ¥ 630 ¥ 1470 ¥ 2100 ¥ 5250 ¥ 21000
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產(chǎn)品包裝(模擬)
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產(chǎn)品包裝(模擬)
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DAB staining on IHC-P; Samples: Human Kidney Tissue; Primary Ab: 10μg/ml Rabbit Anti-Human MGMT Antibody Second Ab: 2μg/mL?HRP-Linked Caprine Anti-Rabbit IgG Polyclonal Antibody (Catalog: SAA544Rb19)
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DAB staining on IHC-P;
Samples: Human Stomach Tissue;
Primary Ab: 10μg/ml Rabbit Anti-Human MGMT Antibody
Second Ab: 2μg/mL?HRP-Linked Caprine Anti-Rabbit IgG Polyclonal Antibody
(Catalog: SAA544Rb19) -
DAB staining on IHC-P;
Sample: Porcine Testis Tissue
Primary Ab: 10μg/ml Rabbit Anti-Human MGMT Antibody
Control: Used PBS instead of primary antibody
Second Ab: 2μg/ml HRP-Linked Caprine Anti-Rabbit IgG Polyclonal Antibody
(Catalog: SAA544Rb19) -
DAB staining on IHC-P;
Sample: Porcine Liver Tissue
Primary Ab: 10μg/ml Rabbit Anti-Human MGMT Antibody
Control: Used PBS instead of primary antibody
Second Ab: 2μg/ml HRP-Linked Caprine Anti-Rabbit IgG Polyclonal Antibody
(Catalog: SAA544Rb19) -
DAB staining on IHC-P;
Sample: Porcine Kidney Tissue
Primary Ab: 10μg/ml Rabbit Anti-Human MGMT Antibody
Control: Used PBS instead of primary antibody
Second Ab: 2μg/ml HRP-Linked Caprine Anti-Rabbit IgG Polyclonal Antibody
(Catalog: SAA544Rb19)
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Figure. Western Blot; Sample: Recombinant MGMT, Human.
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Western Blot; Sample: Jurkat cell lysate
Primary Ab: 0.2μg/ml Rabbit Anti-Human MGMT Antibody
Second Ab: 0.2μg/ml HRP-Linked Caprine Anti-Rabbit IgG Polyclonal Antibody
(Catalog: SAA544Rb19) -
Knockout Varification: <br/>Lane 1: Wild-type Jurkat cell lysate; <br/>Lane 2: MGMT knockout Jurkat cell lysate; <br/>Predicted MW: 21kd <br/>Observed MW: 22kd<br/>Primary Ab: 3μg/ml Rabbit Anti-Human MGMT Antibody<br/>Second Ab: 0.2μg/mL?HRP-Linked Caprine Anti-Rabbit IgG Polyclonal Antibody<br/>(Catalog: SAA544Rb19)
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通過ISO 9001、ISO 13485質(zhì)量體系認(rèn)證
特異性
該抗體是針對MGMT的兔多克隆抗體。在免疫組織化學(xué)染色和免疫印跡實驗中能識別MGMT。
用法
Western blotting: 0.01-3μg/mL;
Immunohistochemistry: 5-30μg/mL;
Optimal working dilutions must be determined by end user.
儲存
經(jīng)常使用則4°C保存。-20°C保存不超過兩年。避免反復(fù)凍融。
穩(wěn)定性
熱穩(wěn)定性以損失率顯示。損失率是由加速降解試驗決定，具體方法如下：在37°C孵育48小時，沒有顯著的降解或者沉淀產(chǎn)生。保質(zhì)期內(nèi)，在適當(dāng)?shù)臈l件下存儲，損失率低于5%。
贈品
增值服務(wù)
相關(guān)產(chǎn)品
| 編號 | 適用物種：Homo sapiens (Human，人) | 應(yīng)用(僅供研究使用，不用于臨床診斷！) |
| RPC027Hu01 | O-6-甲基鳥嘌呤DNA甲基轉(zhuǎn)移酶(MGMT)重組蛋白 | Positive Control; Immunogen; SDS-PAGE; WB. |
| PAC027Hu01 | O-6-甲基鳥嘌呤DNA甲基轉(zhuǎn)移酶(MGMT)多克隆抗體 | WB,IHC |
| MAC027Hu22 | O-6-甲基鳥嘌呤DNA甲基轉(zhuǎn)移酶(MGMT)單克隆抗體 | WB; IHC; ICC; IP. |
| SEC027Hu | O-6-甲基鳥嘌呤DNA甲基轉(zhuǎn)移酶(MGMT)檢測試劑盒(酶聯(lián)免疫吸附試驗法) | Enzyme-linked immunosorbent assay for Antigen Detection. |
| LMC027Hu | O-6-甲基鳥嘌呤DNA甲基轉(zhuǎn)移酶(MGMT)等多因子檢測試劑盒(流式熒光發(fā)光法) | FLIA Kit for Antigen Detection. |
參考文獻(xiàn)
| 雜志 | 參考文獻(xiàn) |
| Biotechnology and Bioengineering | Targeted nitric oxide delivery preferentially induces glioma cell chemosensitivity via altered p53 and O(6) -Methylguanine-DNA Methyltransferase activity [PubMed: 23125026] |
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