載脂蛋白E(APOE)多克隆抗體
Polyclonal Antibody to Apolipoprotein E (APOE)
Apo-E; AD2; Apoprotein; Alzheimer Disease 2(E4-Associated,Late Onset
- 編號PAA704Ra01
- 物種Rattus norvegicus (Rat，大鼠) 相同的名稱，不同的物種。
- 來源多抗制備
- 宿主兔
- 效價-
- Ig類型 IgG
- 純化方式抗原特異性親和純化
- 標(biāo)記物無標(biāo)記物
- 免疫原 RPA704Ra01-載脂蛋白E(APOE)重組蛋白
- 緩沖液成份磷酸鹽緩沖液（pH7.4,含有0.02% NaN3和50%甘油）
- 性狀液體
- 濃度1mg/ml
- 且適物種Mus musculus (Mouse，小鼠)
- 應(yīng)用WB; IHC; ICC; IP; FCM
如果抗體需用于流式細(xì)胞術(shù)，請參見流式抗體。 - 下載 英文說明書 中文說明書
- 規(guī)格 20μl100μl 200μl 1ml 10ml
- 價格 ¥ 616 ¥ 1436 ¥ 2052 ¥ 5130 ¥ 20520
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產(chǎn)品包裝(模擬)
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產(chǎn)品包裝(模擬)
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DAB staining on IHC-P; Sample: Rat Kidney Tissue Primary Ab: 10μg/ml Rabbit Anti-Rat APOE Antibody Control: Used PBS instead of primary antibody Second Ab: 2μg/ml HRP-Linked Caprine Anti-Rabbit IgG Polyclonal Antibody (Catalog: SAA544Rb19)
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DAB staining on IHC-P;
Sample: Rat Liver Tissue
Primary Ab: 10μg/ml Rabbit Anti-Rat APOE Antibody
Control: Used PBS instead of primary antibody
Second Ab: 2μg/ml HRP-Linked Caprine Anti-Rabbit IgG Polyclonal Antibody
(Catalog: SAA544Rb19) -
DAB staining on IHC-P;
Samples: Rat Testis Tissue;
Primary Ab: 20μg/ml Rabbit Anti-Rat APOE Antibody
Second Ab: 2μg/mL?HRP-Linked Caprine Anti-Rabbit IgG Polyclonal Antibody
(Catalog: SAA544Rb19) -
DAB staining on IHC-P;
Sample: Rat Cerebrum Tissue
Primary Ab: 10μg/ml Rabbit Anti-Rat APOE Antibody
Control: Used PBS instead of primary antibody
Second Ab: 2μg/ml HRP-Linked Caprine Anti-Rabbit IgG Polyclonal Antibody
(Catalog: SAA544Rb19)
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Figure. Western Blot; Sample: Recombinant APOE, Rat.
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Western Blot; Sample: Rat Lung lysate;
Primary Ab: 1μg/ml Rabbit Anti-Rat APOE Antibody
Second Ab: 0.2μg/mL?HRP-Linked Caprine Anti-Rabbit IgG Polyclonal Antibody
(Catalog: SAA544Rb19) -
Western Blot; Sample: Rat Serum;
Primary Ab: 1μg/ml Rabbit Anti-Rat APOE Antibody
Second Ab: 0.2μg/mL?HRP-Linked Caprine Anti-Rabbit IgG Polyclonal Antibody
(Catalog: SAA544Rb19) -
Western Blot; Sample: Rat Blood Cells lysate;
Primary Ab: 1μg/ml Rabbit Anti-Rat APOE Antibody
Second Ab: 0.2μg/mL?HRP-Linked Caprine Anti-Rabbit IgG Polyclonal Antibody
(Catalog: SAA544Rb19) -
Western Blot; Sample: Rat Liver lysate;
Primary Ab: 1μg/ml Rabbit Anti-Rat APOE Antibody
Second Ab: 0.2μg/mL?HRP-Linked Caprine Anti-Rabbit IgG Polyclonal Antibody
(Catalog: SAA544Rb19) -
Western Blot; Sample: Mouse Serum;
Primary Ab: 1μg/ml Rabbit Anti-Rat APOE Antibody
Second Ab: 0.2μg/mL?HRP-Linked Caprine Anti-Rabbit IgG Polyclonal Antibody
(Catalog: SAA544Rb19) -
Mouse Hepa1-6 cell was fixed with 2% paraformaldehyde (10 min) , permeabilised with 0.1% BSA-Triton X-100,then stained with 20μg/ml rabbit Anti-Mouse APOE Polyclonal Antibody (Catalog PAA704Ra01, red histogram) or Isotype control antibody (Catalog IS067-Rb01, green histogram), followed by 1μg/ml FITC-conjugated Anti-rabbit IgG Secondary Antibody (Catalog SAA544Rb18).
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通過ISO 9001、ISO 13485質(zhì)量體系認(rèn)證
特異性
該抗體是針對APOE的兔多克隆抗體。在免疫組織化學(xué)染色和免疫印跡實驗中能識別APOE。
用法
Western blotting: 0.5-2μg/mL;
Immunohistochemistry: 5-20μg/mL;
Immunocytochemistry: 5-20μg/mL;
Flow cytometry:20μg/ml;
Optimal working dilutions must be determined by end user.
儲存
經(jīng)常使用則4°C保存。-20°C保存不超過兩年。避免反復(fù)凍融。
穩(wěn)定性
熱穩(wěn)定性以損失率顯示。損失率是由加速降解試驗決定，具體方法如下：在37°C孵育48小時，沒有顯著的降解或者沉淀產(chǎn)生。保質(zhì)期內(nèi)，在適當(dāng)?shù)臈l件下存儲，損失率低于5%。
贈品
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相關(guān)產(chǎn)品
| 編號 | 適用物種：Rattus norvegicus (Rat，大鼠) | 應(yīng)用(僅供研究使用，不用于臨床診斷！) |
| RPA704Ra02 | 載脂蛋白E(APOE)重組蛋白 | Positive Control; Immunogen; SDS-PAGE; WB. |
| RPA704Ra01 | 載脂蛋白E(APOE)重組蛋白 | Positive Control; Immunogen; SDS-PAGE; WB. |
| PAA704Ra01 | 載脂蛋白E(APOE)多克隆抗體 | WB; IHC; ICC; IP; FCM |
| PAA704Ra02 | 載脂蛋白E(APOE)多克隆抗體 | WB; IHC; ICC; IP. |
| MAA704Ra22 | 載脂蛋白E(APOE)單克隆抗體 | WB; IHC; ICC; IP. |
| MAA704Ra23 | 載脂蛋白E(APOE)單克隆抗體 | WB; IHC; ICC; IP. |
| MAA704Ra21 | 載脂蛋白E(APOE)單克隆抗體 | WB; IHC; ICC; IP. |
| MAA704Ra24 | 載脂蛋白E(APOE)單克隆抗體 | WB; IHC; ICC; IP. |
| MAA704Ra25 | 載脂蛋白E(APOE)單克隆抗體 | WB; IHC; ICC; IP. |
| MAA704Ra27 | 載脂蛋白E(APOE)單克隆抗體 | WB; IHC; ICC; IP. |
| MAA704Ra29 | 載脂蛋白E(APOE)單克隆抗體 | WB; IHC; ICC; IP. |
| FAA704Ra02 | 抗載脂蛋白E(APOE)單克隆抗體 | Flow cytometry. |
| SEA704Ra | 載脂蛋白E(APOE)檢測試劑盒(酶聯(lián)免疫吸附試驗法) | Enzyme-linked immunosorbent assay for Antigen Detection. |
| LMA704Ra | 載脂蛋白E(APOE)等多因子檢測試劑盒(流式熒光發(fā)光法) | FLIA Kit for Antigen Detection. |
| KSA704Ra01 | 載脂蛋白E(APOE)檢測試劑盒DIY材料(酶聯(lián)免疫吸附試驗法) | Main materials for "Do It (ELISA Kit) Yourself". |
參考文獻(xiàn)
| 雜志 | 參考文獻(xiàn) |
| Seizure - European Journal of Epilepsy | Cerebrospinal fluid apolipoprotein E concentration decreases after seizure [Seizure: PIIS1059131109002441] |
| Biochimica et Biophysica Acta | Hepatic lipase- and endothelial lipase-deficiency in mice promotes macrophage-to-feces RCT and HDL antioxidant properties [PubMed: 23328279 ] |
| Medical Oncology | Expression and clinical significance of apolipoprotein E in pancreatic ductal adenocarcinoma [Pubmed: 23609192] |
| Diabetes. | Adipocyte Atp-Binding Cassette G1 Promotes Triglyceride Storage, Fat Mass Growth And Human Obesity [Pubmed:25249572] |
| renal failure | Frequencies of apolipoprotein E alleles in depressed patients undergoing hemodialysis – a case-control study [PubMed: 25707516] |
| Biochim Biophys Acta | Culturing of HepG2 cells with human serum improve their functionality and suitability in studies of lipid metabolism [PubMed: 26515253] |
| Diabetes | Adipocyte ATP-binding cassette G1 promotes triglyceride storage, fat mass growth, and human obesity [PubMed: 25249572] |
| Scientific Reports | Adaptive?immunity?against?gut?microbiota?enhances?apoE-mediated?immune?regulation?andreduces?atherosclerosis?and?western-diet-related?inflammation. [pubmed:27383250] |
| Biochemical Pharmacology? | Differential effects of apoE and apoJ mimetic peptides on the action of an anti-Aβ scFv in 3xTg-AD mice [Pubmed:30026023] |
| Oncotarget | Deletion of plasma Phospholipid Transfer Protein (PLTP) increases microglial phagocytosis and reduces cerebral amyloid-β deposition in the J20 mouse?… [Pubmed:29731975] |
| Proteomics Clinical Applications | Comparative proteomics analysis of serum proteins in gestational diabetes during early and middle stages of pregnancy [Pubmed: 31162828] |
| Nature Communications | Hepatic HuR modulates lipid homeostasis in response to high-fat diet [Pubmed: 32546794] |
| VETERINARY PATHOLOGY | Pathology and Proteomics-Based Diagnosis of Localized Light-Chain Amyloidosis in Dogs and Cats [Pubmed: 32880234] |
| Journal of Clinical Medicine | The Significance of Apolipoprotein E Measurement in the Screening of Fetal Down Syndrome [33321701] |
| Cancer Cell Int | Discovery and validation of FBLN1 and ANT3 as potential biomarkers for early detection of cervical cancer [33602229] |
| Nat Commun | GP73 is a TBC-domain Rab GTPase-activating protein contributing to the pathogenesis of non-alcoholic fatty liver disease without obesity [34853313] |
| Talanta | Iridium nanoclusters as high sensitive-tunable elemental labels for immunoassays: Determination of IgE and APOE in aqueous humor by inductively coupled?… [Pubmed:35378356] |
| Analytica Chimica Acta | Gold nanoclusters as elemental label for the sequential quantification of apolipoprotein E and metallothionein 2A in individual human cells of the retinal?… [Pubmed:35361429] |
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