分泌型卷曲相關(guān)蛋白5(SFRP5)多克隆抗體
Polyclonal Antibody to Secreted Frizzled Related Protein 5 (SFRP5)
SARP3; Secreted Apoptosis Related Protein 3; Frizzled-related protein 1b
- 編號(hào)PAC842Ra01
- 物種Rattus norvegicus (Rat，大鼠) 相同的名稱(chēng)，不同的物種。
- 來(lái)源多抗制備
- 宿主兔
- 效價(jià)-
- Ig類(lèi)型 IgG
- 純化方式抗原特異性親和純化
- 標(biāo)記物無(wú)標(biāo)記物
- 免疫原 RPC842Ra01-分泌型卷曲相關(guān)蛋白5(SFRP5)重組蛋白
- 緩沖液成份磷酸鹽緩沖液（pH7.4,含有0.02% NaN3和50%甘油）
- 性狀液體
- 濃度500μg/mL
- 且適物種Mus musculus (Mouse，小鼠)
- 應(yīng)用WB; IHC; ICC; IP.
如果抗體需用于流式細(xì)胞術(shù)，請(qǐng)參見(jiàn)流式抗體。 - 下載 英文說(shuō)明書(shū) 中文說(shuō)明書(shū)
- 規(guī)格 20μl100μl 200μl 1ml 10ml
- 價(jià)格 ¥ 684 ¥ 1596 ¥ 2280 ¥ 5700 ¥ 22800
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產(chǎn)品包裝(模擬)
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產(chǎn)品包裝(模擬)
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IHC圖
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Figure. Western Blot; Sample: Recombinant SFRP5, Rat.
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Western Blot; Sample: Mouse Pancreas lysate
Primary Ab: 0.3μg/ml Rabbit Anti-Rat SFRP5 Antibody
Second Ab: 0.2μg/mL HRP-Linked Caprine Anti-Rabbit IgG Polyclonal Antibody
(Catalog: SAA544Rb19)
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通過(guò)ISO 9001、ISO 13485質(zhì)量體系認(rèn)證
特異性
該抗體是針對(duì)SFRP5的兔多克隆抗體。在免疫組織化學(xué)染色和免疫印跡實(shí)驗(yàn)中能識(shí)別SFRP5。
用法
Western blotting: 0.5-2ug/ml
Immunocytochemistry in formalin fixed cells: 5-20ug/ml
Immunohistochemistry in formalin fixed frozen section: 5-20ug/ml
Immunohistochemistry in paraffin section: 5-20ug/ml
Enzyme-linked Immunosorbent Assay: 0.05-2ug/ml
Optimal working dilutions must be determined by end user.
儲(chǔ)存
經(jīng)常使用則4°C保存。-20°C保存不超過(guò)兩年。避免反復(fù)凍融。
穩(wěn)定性
熱穩(wěn)定性以損失率顯示。損失率是由加速降解試驗(yàn)決定，具體方法如下：在37°C孵育48小時(shí)，沒(méi)有顯著的降解或者沉淀產(chǎn)生。保質(zhì)期內(nèi)，在適當(dāng)?shù)臈l件下存儲(chǔ)，損失率低于5%。
贈(zèng)品
增值服務(wù)
抗體標(biāo)記定制服務(wù)
Protein-A/G純化柱
細(xì)胞和組織染色液
抗體陽(yáng)性對(duì)照品
組織/切片定制服務(wù)
磷酸化抗體定制服務(wù)
免疫印跡(WB)實(shí)驗(yàn)服務(wù)
免疫組織化學(xué)(IHC)實(shí)驗(yàn)服務(wù)
免疫細(xì)胞化學(xué)(ICC)實(shí)驗(yàn)服務(wù)
流式細(xì)胞術(shù)(FCM)實(shí)驗(yàn)服務(wù)
免疫沉淀(IP)實(shí)驗(yàn)服務(wù)
免疫熒光(IF)實(shí)驗(yàn)服務(wù)
緩沖液
DAB顯色試劑盒
SABC試劑盒
長(zhǎng)臂生物素標(biāo)記試劑盒
實(shí)時(shí)熒光定量PCR實(shí)驗(yàn)服務(wù)
相關(guān)產(chǎn)品
| 編號(hào) | 適用物種：Rattus norvegicus (Rat，大鼠) | 應(yīng)用(僅供研究使用，不用于臨床診斷！) |
| RPC842Ra01 | 分泌型卷曲相關(guān)蛋白5(SFRP5)重組蛋白 | Positive Control; Immunogen; SDS-PAGE; WB. |
| PAC842Ra01 | 分泌型卷曲相關(guān)蛋白5(SFRP5)多克隆抗體 | WB; IHC; ICC; IP. |
| LAC842Ra71 | 分泌型卷曲相關(guān)蛋白5(SFRP5)多克隆抗體(生物素標(biāo)記) | WB; IHC; ICC. |
| MAC842Ra21 | 分泌型卷曲相關(guān)蛋白5(SFRP5)單克隆抗體 | WB; IHC; ICC; IP. |
| SEC842Ra | 分泌型卷曲相關(guān)蛋白5(SFRP5)檢測(cè)試劑盒(酶聯(lián)免疫吸附試驗(yàn)法) | Enzyme-linked immunosorbent assay for Antigen Detection. |
| LMC842Ra | 分泌型卷曲相關(guān)蛋白5(SFRP5)等多因子檢測(cè)試劑盒(流式熒光發(fā)光法) | FLIA Kit for Antigen Detection. |
參考文獻(xiàn)
| 雜志 | 參考文獻(xiàn) |
| Diabetes Research and Clinical Practice | Plasma SFRP5 levels are decreased in Chinese subjects with obesity and type 2 diabetes and negatively correlated with parameters of insulin resistance [PubMed: 23290274] |
| European Journal of Clinical Investigation | Sfrp5 correlates with insulin resistance and oxidative stress [PubMed: 23398169] |
| Pediatric Research | Balanced duo of anti-inflammatory SFRP5 and proinflammatory WNT5A in children [Pubmed: 24603290] |
| Clin Endocrinol (Oxf). | SFRP5 correlates with obesity and metabolic syndrome and increases after weight loss in children [Pubmed: 24330025] |
| The Journal of Clinical Endocrinology & Metabolism | Circulating Sfrp5 is a signature of obesity-related metabolic disorders and is regulated by glucose and liraglutide in humans [Pubmed: 23185036] |
| Atherosclerosis | Low serum level of secreted frizzled-related protein 5, an anti-inflammatory adipokine, is associated with coronary artery disease [Pubmed: 24530778] |
| PLoS One. | Identification of adipokine clusters related to parameters of fat mass, insulin sensitivity and inflammation [Pubmed:Pmc4072672] |
| J Clin Endocrinol Metab. | Activation of noncanonical Wnt signaling through WNT5A in visceral adipose tissue of obese subjects is related to inflammation [Pubmed:24840810] |
| Oncology Letters | Serum secreted frizzled?related protein 5 levels differentially decrease in patients with hepatitis B virus?associated chronic infection and hepatocellular carcinoma [Pubmed:25120720] |
| Diabetes Metab Res Rev. | Circulating SFRP5 levels are elevated in drug‐na?ve recently diagnosed type 2 diabetic patients as compared with prediabetic subjects and controls [Pubmed:25139699] |
| Clinical & Experimental Immunology | The wingless-related integration site-5a/secreted frizzled-related protein-6 system is dysregulated in human sepsis [Pubmed:25382802] |
| Diabetes Metab Res Rev | Circulating SFRP5 levels are elevated in drug-na [PubMed: 25139699] |
| J Matern Fetal Neonatal Med | Prediction of gestational diabetes mellitus by first trimester serum secreted frizzle-related protein-5 levels [PubMed: 26100762] |
| Eur J Clin Invest | Sfrp5 associates with beta-cell function in humans [Pubmed:27019073] |
| Osteoporosis International | Bone marrow sFRP5 level is negatively associated with bone formation markers [pubmed:27986984] |
| Diabetes/Metabolism Research and Reviews | Plasma Sfrp5 levels correlate with determinants of the metabolic syndrome in Chinese adults [pubmed:28303637] |
| Cardiovascular Diabetology | Inverse associations between serum levels of secreted frizzled-related protein-5 (SFRP5) and multiple cardiometabolic risk factors: KORA F4 study [pubmed:28851362] |
| Arteriosclerosis, Thrombosis, and?Vascular Biology? | Effect of SFRP5 (Secreted Frizzled–Related Protein 5) on the WNT5A (Wingless-Type Family Member 5A)-Induced Endothelial Dysfunction and Its Relevance With?… [Pubmed:29674475] |
| Obesity | Longitudinal Increases in Adiposity Contribute to Worsening Adipokine Profile over Time in Mexican Americans [Pubmed:29427376] |
| J Bas Res Med Sci | Decreased level of the anti-inflammatory adipokines, secreted frizzled-related protein 5 and adiponectin, in high cholesterol diet-induced atherosclerotic rats [article-1-352-en.pdf] |
| Molecular?Biology?Reports | The effect of serum and follicular fluid secreted frizzle-related protein-5 on in vitro fertilization outcomes in patients with polycystic ovary syndrome [Pubmed: 30194556] |
| Macrophage LRP1 promotes diet-induced hepatic inflammation and metabolic dysfunction by modulating Wnt signaling [] | |
| British?Journal Of Nutrition | A diet high in sugar-sweetened beverage and low in fruits and vegetables is associated with adiposity and a pro-inflammatory adipokine profile [Pubmed: 30375290] |
| J Clin Endocrinol Metab | Role of wnt5a in Metabolic Inflammation in Humans [Pubmed: 30137542] |
| Frontiers in Endocrinology | Association of Adipocytokines With Carotid Intima Media Thickness and Arterial Stiffness in Obstructive Sleep Apnea Patients [Pubmed: 32300333] |
| CLINICAL BIOCHEMISTRY | Sfrp5/Wnt5a and Leptin/adiponectin levels in the serum and the periarterial adipose tissue of patients with peripheral arterial occlusive disease [Pubmed: 33188773] |
| Sao Paulo Medical Journal | Association of serum levels of secreted frizzled-related protein 5 and Wnt member 5a with glomerular filtration rate in patients with type 2 diabetes mellitus and … [Pubmed: 32491086] |
| Kidney Blood Press Res | Secreted Frizzled-Related Protein 5 Ameliorates Vascular Calcification in a Rat Model of Chronic Kidney Disease through the Wnt/β-Catenin Pathway [34469882] |
| Cytokine | Association of serum uromodulin with adipokines in dependence of type 2 diabetes [34920231] |
| PLoS One | Association of circulating MR-proADM with all-cause and cardiovascular mortality in the general population: Results from the KORA F4 cohort study [Pubmed:34990470] |
| JMIR Res Protoc | Investigating New Sensory Methods Related to Taste Sensitivity, Preferences, and Diet of Mother-Infant Pairs and Their Relationship With Body Composition?… [Pubmed:35475790] |
| Diabetology | Early Pregnancy Serum Concentration of Secreted Frizzled-Related Protein 4, Secreted Frizzled-Related Protein 5, and Chemerin in Obese Women Who Develop?… [] |
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