α1-酸性糖蛋白(a1AGP)檢測試劑盒DIY材料(酶聯(lián)免疫吸附試驗法)
ELISA Kit DIY Materials for Alpha-1-Acid Glycoprotein (a1AGP)
AGP1; a1-AGP; ORM1; OMD 1; Orosomucoid 1
- 編號KSA816Ra01
- 物種Rattus norvegicus (Rat,大鼠) 相同的名稱,不同的物種。
- 試劑內(nèi)容 包被抗體、檢測抗體、標(biāo)準(zhǔn)品、HRP標(biāo)記的鏈霉親和素、TMB底物、96孔板
- 可檢測樣本Serum, plasma, tissue homogenates, cell lysates, cell culture supernates and other biological fluids.
- 可適用原理雙抗夾心酶聯(lián)免疫吸附試驗法測抗原
- 可檢測范圍6.25-400ng/mL
- 可適靈敏度2.88ng/mL
- 應(yīng)用Main materials for "Do It (ELISA Kit) Yourself".
- 下載 英文說明書 中文說明書
- 規(guī)格 96T*596T*10 96T*20 96T*50 96T*100
- 價格 ¥ 7387 ¥ 12312 ¥ 22162 ¥ 43092 ¥ 73872
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特異性
試劑盒中的抗體對α1-酸性糖蛋白(a1AGP)具有高靈敏度和高特異性,經(jīng)檢測與其它相似物質(zhì)無明顯交叉反應(yīng)。
用法
1.用100μL每孔的工作濃度的包被抗體包被96孔板,4度孵育過夜或者37度孵育2小時;
2.洗板1次;
3.用200μL每孔的工作濃度的封閉液,37度孵育1.5小時;
4.洗板1次。酶標(biāo)板即可用于ELISA實驗,后續(xù)使用方法與ELISA同。
儲存
抗體,標(biāo)準(zhǔn)品和HRP標(biāo)記的鏈霉親和素在-20度保存,TMB需在4度保存,96孔板在室溫保存。所有組分保質(zhì)期為1年,試劑一旦開封,在4度保存時間為1個月。
配套試劑
增值服務(wù)
相關(guān)產(chǎn)品
編號 | 適用物種:Rattus norvegicus (Rat,大鼠) | 應(yīng)用(僅供研究使用,不用于臨床診斷!) |
RPA816Ra02 | α1-酸性糖蛋白(a1AGP)重組蛋白 | Positive Control; Immunogen; SDS-PAGE; WB. |
RPA816Ra03 | α1-酸性糖蛋白(a1AGP)重組蛋白 | Positive Control; Immunogen; SDS-PAGE; WB. |
RPA816Ra01 | α1-酸性糖蛋白(a1AGP)重組蛋白 | Positive Control; Immunogen; SDS-PAGE; WB. |
PAA816Ra02 | α1-酸性糖蛋白(a1AGP)多克隆抗體 | WB; IHC; ICC; IP. |
PAA816Ra03 | α1-酸性糖蛋白(a1AGP)多克隆抗體 | WB; IHC; ICC; IP. |
PAA816Ra01 | α1-酸性糖蛋白(a1AGP)多克隆抗體 | WB; IHC; ICC; IP. |
LAA816Ra71 | α1-酸性糖蛋白(a1AGP)多克隆抗體(生物素標(biāo)記) | WB; IHC; ICC. |
MAA816Ra23 | α1-酸性糖蛋白(a1AGP)單克隆抗體 | WB; IHC; ICC; IP. |
MAA816Ra27 | α1-酸性糖蛋白(a1AGP)單克隆抗體 | WB; IHC; ICC; IP. |
MAA816Ra25 | α1-酸性糖蛋白(a1AGP)單克隆抗體 | IHC |
MAA816Ra24 | α1-酸性糖蛋白(a1AGP)單克隆抗體 | WB; IHC; ICC; IP. |
MAA816Ra26 | α1-酸性糖蛋白(a1AGP)單克隆抗體 | WB; IHC; ICC; IP. |
MAA816Ra21 | α1-酸性糖蛋白(a1AGP)單克隆抗體 | WB; IHC; ICC; IP. |
MAA816Ra22 | α1-酸性糖蛋白(a1AGP)單克隆抗體 | WB; IHC; ICC; IP. |
FAA816Ra02 | 抗α1-酸性糖蛋白(a1AGP)單克隆抗體 | Flow cytometry. |
LAA816Ra76 | α1-酸性糖蛋白(a1AGP)單克隆抗體(生物素標(biāo)記) | WB; IHC; ICC. |
SEA816Ra | α1-酸性糖蛋白(a1AGP)檢測試劑盒(酶聯(lián)免疫吸附試驗法) | Enzyme-linked immunosorbent assay for Antigen Detection. |
LMA816Ra | α1-酸性糖蛋白(a1AGP)等多因子檢測試劑盒(流式熒光發(fā)光法) | FLIA Kit for Antigen Detection. |
PSA816Ra01 | α1-酸性糖蛋白(a1AGP)抗體對 | ELISA; CLIA; ELISPOT; Luminex; Immunochromatography and other Immunoassays. |
KSA816Ra01 | α1-酸性糖蛋白(a1AGP)檢測試劑盒DIY材料(酶聯(lián)免疫吸附試驗法) | Main materials for "Do It (ELISA Kit) Yourself". |
參考文獻
雜志 | 參考文獻 |
J Reprod Dev.? | An Acute-phase Protein as a Regulator of Sperm Survival in the Bovine Oviduct: Alpha 1-acid-glycoprotein Impairs Neutrophil Phagocytosis of Sperm In Vitro [Pubmed:Pmc4219990] |
Molecular Reproduction and Development | Evidence for a novel, local acute-phase response in the bovine oviduct: Progesterone and lipopolysaccharide up-regulate alpha 1-acid-glycoprotein expression in epithelial cells in vitro [Pubmed:25123565] |
BMC Complementary and Alternative Medicine | Identification of plasma protein markers common to patients with malignant tumour and Abnormal Savda in Uighur medicine: a prospective clinical study [Pubmed:25652121] |
PLoS One | Gut Microbial Dysbiosis May Predict Diarrhea and Fatigue in Patients Undergoing Pelvic Cancer Radiotherapy: A Pilot Study [PubMed: 25955845] |
Sci Rep.? | S100A9 and ORM1 serve as predictors of therapeutic response and prognostic factors in advanced extranodal NK/T cell lymphoma patients treated with pegaspargase/gemcitabine [pmc:PMC4810364] |
Journal of Chromatography A | Application of a new procedure for liquid chromatography/mass spectrometry profiling of plasma amino acid-related metabolites and untargeted shotgun proteomics to identify mechanisms and biomarkers of calcific aortic stenosis [S0021967317311858] |
Scientific Reports | An autoregressive logistic model to predict the reciprocal effects of oviductal fluid components on in vitro spermophagy by neutrophils in cattle [pubmed:28667317] |
33 | Transcriptome Analysis Uncovers a Growth-Promoting Activity of Orosomucoid-1 on Hepatocytes. [pubmed:28927749] |
Brazilian Journal of Otorhinolaryngology | Inflammatory markers in palatine tonsils of children with obstructive sleep apnea syndrome [Pubmed: 30213594] |
BioRxiv | Proteome-wide characterization and biomarker identification of intracranial aneurysm [] |
The FEBS journal | Dramatically changed immune‐related molecules as early diagnostic biomarkers of non‐small cell lung cancer [Pubmed: 31482685] |
PLoS One | Effects of vaccination on acute-phase protein response in broiler chicken [Pubmed: 32191767] |
Indian Journal of Animal Sciences | Peripheral concentrations of metabolic and inflammatory indicators during transition period and their relationship with postpartum clinical endometritis in dairy?… [] |
A New Biomarker Tool for Risk Stratification in ?°De Novo?± Acute Heart Failure (OROME) [] |
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