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α2-HS糖蛋白(AHSG)檢測(cè)試劑盒DIY材料(酶聯(lián)免疫吸附試驗(yàn)法)

ELISA Kit DIY Materials for Alpha-2-Heremans Schmid Glycoprotein (AHSG)

aHSG; HSGa; A2HS; AHS; FETUA; HSG-A2; Fetuin-A; Alpha 2-HS Glycoprotein; Fetuin A; Alpha-2-Z-Globulin; Ba-alpha-2-Glycoprotein

  • α2-HS糖蛋白(AHSG)檢測(cè)試劑盒DIY材料(酶聯(lián)免疫吸附試驗(yàn)法) 產(chǎn)品包裝(模擬)
  • α2-HS糖蛋白(AHSG)檢測(cè)試劑盒DIY材料(酶聯(lián)免疫吸附試驗(yàn)法) 產(chǎn)品包裝(模擬)
  • Certificate 通過ISO 9001、ISO 13485質(zhì)量體系認(rèn)證

特異性

試劑盒中的抗體對(duì)α2-HS糖蛋白(aHSG)具有高靈敏度和高特異性,經(jīng)檢測(cè)與其它相似物質(zhì)無明顯交叉反應(yīng)。

用法

1.用100μL每孔的工作濃度的包被抗體包被96孔板,4度孵育過夜或者37度孵育2小時(shí);
2.洗板1次;
3.用200μL每孔的工作濃度的封閉液,37度孵育1.5小時(shí);
4.洗板1次。酶標(biāo)板即可用于ELISA實(shí)驗(yàn),后續(xù)使用方法與ELISA同。

儲(chǔ)存

抗體,標(biāo)準(zhǔn)品和HRP標(biāo)記的鏈霉親和素在-20度保存,TMB需在4度保存,96孔板在室溫保存。所有組分保質(zhì)期為1年,試劑一旦開封,在4度保存時(shí)間為1個(gè)月。

配套試劑

相關(guān)產(chǎn)品

編號(hào) 適用物種:Bos taurus; Bovine (Cattle,牛) 應(yīng)用(僅供研究使用,不用于臨床診斷!)
RPA178Bo01 α2-HS糖蛋白(AHSG)重組蛋白 Positive Control; Immunogen; SDS-PAGE; WB.
PAA178Bo01 α2-HS糖蛋白(AHSG)多克隆抗體 WB; IHC; ICC; IP.
MAA178Bo21 α2-HS糖蛋白(AHSG)單克隆抗體 WB; IHC; ICC; IP.
SEA178Bo α2-HS糖蛋白(AHSG)檢測(cè)試劑盒(酶聯(lián)免疫吸附試驗(yàn)法) Enzyme-linked immunosorbent assay for Antigen Detection.
LMA178Bo α2-HS糖蛋白(AHSG)等多因子檢測(cè)試劑盒(流式熒光發(fā)光法) FLIA Kit for Antigen Detection.
KSA178Bo01 α2-HS糖蛋白(AHSG)檢測(cè)試劑盒DIY材料(酶聯(lián)免疫吸附試驗(yàn)法) 自己動(dòng)手制作ELISA試劑盒的主要材料
PGA178Bo01 α2-HS糖蛋白(AHSG)引物對(duì) PCR
CGA178Bo01 α2-HS糖蛋白(AHSG)cDNA PCR、蛋白表達(dá)等

參考文獻(xiàn)

雜志 參考文獻(xiàn)
Journal of Proteomics Proteomic analysis reveals oxidative stress response as the main adaptative physiological mechanism in cows under different production systems [ScienceDirect: S1874391912002084]
Journal of Physiology and Biochemistry Curcumin prevents liver fat accumulation and serum fetuin-A increase in rats fed a high-fat diet [PubMed: 23430567]
BioMed Research International Serum Fetuin-A Levels Related with Microalbuminuria in Diet-Induced Obese Rats [PubMed: PMC3591142]
Osteoporosis International Estrogen increases the transcription of human α2-Heremans-Schmid-glycoprotein by an interplay of estrogen receptor α and activator protein-1. [Pubmed: 24504099]
BMC Pharmacology and Toxicology A novel 11β-hydroxysteroid dehydrogenase type1 inhibitor CNX-010-49 improves hyperglycemia, lipid profile and reduces body weight in diet induced obese C57B6/J mice with a potential to provide cardio protective benefits [Pubmed:25098735]
Acta Dermatovenerologica Croatica Relationship Between Serum Fetuin-A Levels and Carotid Intima-media Thickness in Turkish Patients with Mild to Moderate Psoriasis. A Case-control Study [PubMed: 26476900]
Mol Cell Endocrinol Fetuin A promotes lipotoxicity in β cells through the TLR4 signaling pathway and the role of pioglitazone in anti-lipotoxicity. [PubMed: 25986658]
Ann Dermatol Evaluation of Arterial Stiffness in Patients with Beh [PubMed: 26719639]
Endocrine Visceral and subcutaneous adipose tissue express and secrete functional alpha2hsglycoprotein (fetuin a) especially in obesity [pubmed:27738888]
Diabetes Care Targeted Microbiome Intervention by Microencapsulated Delayed-Release Niacin Beneficially Affects Insulin Sensitivity in Humans [pubmed:29212824]
JOURNAL OF?ALZHEIMERS?DISEASE Acute Anti-Inflammatory Markers ITIH4 and AHSG in Mice Brain of a Novel Alzheimer's Disease Model [Pubmed: 30958362]
Journal of Alzheimer's Disease A New Serum Biomarker Set to Detect Mild Cognitive Impairment and Alzheimer's Disease by Peptidome Technology [Pubmed: 31771070]
Sci Rep Ileal transposition helps to regulate plasma hepatokine levels in obese Zucker (Crl: ZUC (ORL)-Lepr fa) rats [33833309]
J Obstet Gynaecol Res Pred?ct?ve value of pregnancy of foll?cular flu?d fetu?n‐A and‐B levels ?n infert?le women after intra‐cytoplasmic sperm injection [34708901]
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