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天冬氨酸轉(zhuǎn)氨酶(AST)檢測試劑盒DIY材料(酶聯(lián)免疫吸附試驗法)

ELISA Kit DIY Materials for Aspartate Aminotransferase (AST)

cCAT; AAT; ASAT; SGOT; GOT1; Cysteine transaminase, cytoplasmic; Transaminase A; Aspartate Transaminase 1,Cytoplasmic; Glutamic-Oxaloacetic Transaminase 1,Soluble

  • 天冬氨酸轉(zhuǎn)氨酶(AST)檢測試劑盒DIY材料(酶聯(lián)免疫吸附試驗法) 產(chǎn)品包裝(模擬)
  • 天冬氨酸轉(zhuǎn)氨酶(AST)檢測試劑盒DIY材料(酶聯(lián)免疫吸附試驗法) 產(chǎn)品包裝(模擬)
  • Certificate 通過ISO 9001、ISO 13485質(zhì)量體系認(rèn)證

特異性

試劑盒中的抗體對天冬氨酸轉(zhuǎn)氨酶(AST)具有高靈敏度和高特異性，經(jīng)檢測與其它相似物質(zhì)無明顯交叉反應(yīng)。

用法

1.用100μL每孔的工作濃度的包被抗體包被96孔板，4度孵育過夜或者37度孵育2小時；
2.洗板1次；
3.用200μL每孔的工作濃度的封閉液,37度孵育1.5小時；
4.洗板1次。酶標(biāo)板即可用于ELISA實驗，后續(xù)使用方法與ELISA同。

儲存

抗體，標(biāo)準(zhǔn)品和HRP標(biāo)記的鏈霉親和素在-20度保存，TMB需在4度保存，96孔板在室溫保存。所有組分保質(zhì)期為1年，試劑一旦開封，在4度保存時間為1個月。

配套試劑

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編號 適用物種：Homo sapiens (Human，人) 應(yīng)用(僅供研究使用，不用于臨床診斷！)
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參考文獻(xiàn)

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Kidney International Adrenocorticotropic hormone ameliorates acute kidney injury by steroidogenic-dependent and-independent mechanisms [Pubmed: 23325074]
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Journal of Immunology The P2X1 Receptor Is Required for Neutrophil Extravasation during Lipopolysaccharide-Induced Lethal Endotoxemia in Mice [Pubmed:25480563]
Gastroenterol Res Pract Assessing the Effect of Leptin on Liver Damage in Case of Hepatic Injury Associated with Paracetamol Poisoning [PubMed: 26697061]
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Scientific Reports Adiponectin?protects?the?rats?liver?against?chronic?intermittent?hypoxia?induced?injury?throughAMP-activated?protein?kinase?pathway. [pubmed:27678302]
?Biochimica et Biophysica Acta (BBA)-General Subjects Modulation of gut microbiota contributes to curcumin-mediated attenuation of hepatic steatosis in rats [pubmed:28341485]
Scientific Reports Deletion of MCP-1 Impedes Pathogenesis of Acid Ceramidase Deficiency [Pubmed:29379059]
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laboratory investigation Hepatic pathology and altered gene transcription in a murine model of acid ceramidase deficiency [Pubmed: 31186526]
Brain Behavior and Immunity Corticosterone-mediated microglia activation affects dendritic spine plasticity and motor learning functions in minimal hepatic encephalopathy [Pubmed: 31437533]
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