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骨成型蛋白2(BMP2)檢測(cè)試劑盒DIY材料(酶聯(lián)免疫吸附試驗(yàn)法)

ELISA Kit DIY Materials for Bone Morphogenetic Protein 2 (BMP2)

BMP2A; BMP-2A; Hemochromatosis Modifier

  • 骨成型蛋白2(BMP2)檢測(cè)試劑盒DIY材料(酶聯(lián)免疫吸附試驗(yàn)法) 產(chǎn)品包裝(模擬)
  • 骨成型蛋白2(BMP2)檢測(cè)試劑盒DIY材料(酶聯(lián)免疫吸附試驗(yàn)法) 產(chǎn)品包裝(模擬)
  • Certificate 通過(guò)ISO 9001、ISO 13485質(zhì)量體系認(rèn)證

特異性

試劑盒中的抗體對(duì)骨成型蛋白2(BMP2)具有高靈敏度和高特異性，經(jīng)檢測(cè)與其它相似物質(zhì)無(wú)明顯交叉反應(yīng)。

用法

1.用100μL每孔的工作濃度的包被抗體包被96孔板，4度孵育過(guò)夜或者37度孵育2小時(shí)；
2.洗板1次；
3.用200μL每孔的工作濃度的封閉液,37度孵育1.5小時(shí)；
4.洗板1次。酶標(biāo)板即可用于ELISA實(shí)驗(yàn)，后續(xù)使用方法與ELISA同。

儲(chǔ)存

抗體，標(biāo)準(zhǔn)品和HRP標(biāo)記的鏈霉親和素在-20度保存，TMB需在4度保存，96孔板在室溫保存。所有組分保質(zhì)期為1年，試劑一旦開封，在4度保存時(shí)間為1個(gè)月。

配套試劑

相關(guān)產(chǎn)品

編號(hào) 適用物種：Rattus norvegicus (Rat，大鼠) 應(yīng)用(僅供研究使用，不用于臨床診斷！)
EPA013Ra61 骨成型蛋白2(BMP2)真核蛋白 Positive Control; Immunogen; SDS-PAGE; WB.
RPA013Ra01 骨成型蛋白2(BMP2)重組蛋白 Positive Control; Immunogen; SDS-PAGE; WB.
PAA013Ra01 骨成型蛋白2(BMP2)多克隆抗體 WB; IHC; ICC; IP.
LAA013Ra71 骨成型蛋白2(BMP2)多克隆抗體(生物素標(biāo)記) WB; IHC; ICC.
MAA013Ra21 骨成型蛋白2(BMP2)單克隆抗體 WB; IHC; ICC; IP.
SEA013Ra 骨成型蛋白2(BMP2)檢測(cè)試劑盒(酶聯(lián)免疫吸附試驗(yàn)法) Enzyme-linked immunosorbent assay for Antigen Detection.
SCA013Ra 骨成型蛋白2(BMP2)檢測(cè)試劑盒(化學(xué)發(fā)光免疫分析法) Chemiluminescent immunoassay for Antigen Detection.
LMA013Ra 骨成型蛋白2(BMP2)等多因子檢測(cè)試劑盒(流式熒光發(fā)光法) FLIA Kit for Antigen Detection.
KSA013Ra01 骨成型蛋白2(BMP2)檢測(cè)試劑盒DIY材料(酶聯(lián)免疫吸附試驗(yàn)法) 自己動(dòng)手制作ELISA試劑盒的主要材料

參考文獻(xiàn)

雜志 參考文獻(xiàn)
Journal of Applied Toxicology Chronic exposure to low concentrations of strontium 90 affects bone physiology but not the hematopoietic system in mice [Wiley: Source]
PLoS ONE A Combinatorial Relative Mass Value Evaluation of Endogenous Bioactive Proteins in Three-Dimensional Cultured Nucleus Pulposus Cells of Herniated Intervertebral Discs: Identification of Potential Target Proteins for Gene Therapeutic Approaches [Plosone: Source]
Medical Journal of Chinese People's Liberation Army Effect of transplantation of BMP-2-induced bone marrow mesenchymal stem cells on myocardial infarction of rats after reperfusion [Source]
PLOS ONE PDGF-AA promotes osteogenic differentiation and migration of mesenchymal stem cell by down-regulating PDGFRα and derepressing BMP-Smad1/5/8 signaling [Pubmed:25470749]
Eur J Pharmacol Negative effect of serotonin–norepinephrine reuptake inhibitor therapy on rat bone tissue after orchidectomy [PubMed: 25934570]
Pharmacology Effect of Mirtazapine on Rat Bone Tissue after Orchidectomy [PubMed: 25871861]
Journal of Cellular Biochemistry Titanium with Nanotopography Induces Osteoblast Differentiation by Regulating Endogenous Bone Morphogenetic Protein Expression and Signaling Pathway [PubMed: 26681207]
International Journal of Nanomedicine Sustained dual release of placental growth factor-2 and bone morphogenic protein-2 from heparin-based nanocomplexes for direct osteogenesis [Pubmed:27042064]
Research report EFFECTS OF FLUORIDE ON THE EXPRESSION OF BMP-2 AND SMAD1 IN RAT OSTEOBLASTS IN VITRO [files:p013-022]
Gene? Unveiling the interactions among BMPR-2, ALK-1 and 5-HTT genes in the pathophysiology of HAPE [Pubmed:27196063]
Journal of Materials Chemistry B Extracellular Matrix Powder from Cultured Cartilage-Like Tissue as Cell Carriers for Cartilage Repair [DOI:10.1039/C7TB00640C]
Scientific Reports 3D- Printed Poly(ε-caprolactone) Scaffold Integrated with Cell-laden Chitosan Hydrogels for Bone Tissue Engineering. [pubmed:29042614]
Journal of Applied Biomaterials & Functional Materials Demineralized dentin and enamel matrices as suitable substrates for bone regeneration [pubmed:28731486]
Journal of?Clinical?Periodontology? Peroxisome proliferator‐activated receptor γ plays dual roles on experimental periodontitis in rats [Pubmed:29574908]
Pharmacology Effects of Amlodipine on Bone Metabolism in Orchidectomised Spontaneously Hypertensive Rats [Pubmed:29898457]
Journal of?Applied?Biomaterials?&?Functional?Materials BMP-2 and type I collagen preservation in human deciduous teeth after demineralization [Pubmed:30045659]
Applied Surface Science Enhanced osteogenic differentiation of MC3T3-E1 on rhBMP-2 immobilized titanium surface through polymer-mediated electrostatic interaction []
Materials Science and Engineering: C Injectable hydrogels from enzyme-catalyzed crosslinking as BMSCs-laden scaffold for bone repair and regeneration []
Biomaterials Science Development of CaCO 3 microsphere-based composite hydrogel for dual delivery of growth factor and Ca to enhance bone regeneration []
ENVIRONMENTAL TOXICOLOGY AND PHARMACOLOGY The dose-time effects of fluoride on the expression and DNA methylation level of the promoter region of BMP-2 and BMP-7 in rats [Pubmed: 32004919]
Journal of Industrial and Engineering Chemistry Biofabrication and application of decellularized bone extracellular matrix for effective bone regeneration []
BMC Musculoskeletal Disorders Synergistic Effect of Platelet-rich Fibrin Releasate and Bone Marrow Stem Cells in Preventing Bone Loss in Ovariectomized Mouse []
Bioact Mater New design to remove leukocytes from platelet-rich plasma (PRP) based on cell dimension rather than density [33842739]
Tissue Eng Regen Med Osteogenic and Angiogenic Potency of VEGF165-Transfected Canine Bone Marrow Mesenchymal Cells Combined with Coral Hydroxyapatite in Vitro [34302695]
Toxicon The WNT/β-catenin signalling pathway induces chondrocyte apoptosis in the cartilage injury caused by T-2 toxin in rats [34742779]
ACS Biomater Sci Eng Polydopamine-Coated Poly(l-lactide) Nanofibers with Controlled Release of VEGF and BMP-2 as a Regenerative Periosteum [34472855]
Phytomedicine Efficacy of Total flavonoids of Rhizoma drynariae on the Blood Vessels and the Growth Quality of Bone Graft in the Induced Membrane [Pubmed:35278899]
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