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軟骨寡聚基質(zhì)蛋白(COMP)檢測(cè)試劑盒DIY材料(酶聯(lián)免疫吸附試驗(yàn)法)

ELISA Kit DIY Materials for Cartilage Oligomeric Matrix Protein (COMP)

MED; EDM1; EPD1; PSACH; THBS5; TSP-5; Pseudoachondroplasia,Epiphyseal Dysplasia 1,Multiple; Thrombospondin 5

  • 軟骨寡聚基質(zhì)蛋白(COMP)檢測(cè)試劑盒DIY材料(酶聯(lián)免疫吸附試驗(yàn)法) 產(chǎn)品包裝(模擬)
  • 軟骨寡聚基質(zhì)蛋白(COMP)檢測(cè)試劑盒DIY材料(酶聯(lián)免疫吸附試驗(yàn)法) 產(chǎn)品包裝(模擬)
  • Certificate 通過(guò)ISO 9001、ISO 13485質(zhì)量體系認(rèn)證

特異性

試劑盒中的抗體對(duì)軟骨寡聚基質(zhì)蛋白(COMP)具有高靈敏度和高特異性,經(jīng)檢測(cè)與其它相似物質(zhì)無(wú)明顯交叉反應(yīng)。

用法

1.用100μL每孔的工作濃度的包被抗體包被96孔板,4度孵育過(guò)夜或者37度孵育2小時(shí);
2.洗板1次;
3.用200μL每孔的工作濃度的封閉液,37度孵育1.5小時(shí);
4.洗板1次。酶標(biāo)板即可用于ELISA實(shí)驗(yàn),后續(xù)使用方法與ELISA同。

儲(chǔ)存

抗體,標(biāo)準(zhǔn)品和HRP標(biāo)記的鏈霉親和素在-20度保存,TMB需在4度保存,96孔板在室溫保存。所有組分保質(zhì)期為1年,試劑一旦開(kāi)封,在4度保存時(shí)間為1個(gè)月。

配套試劑

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參考文獻(xiàn)

雜志 參考文獻(xiàn)
Chinese Medical Journal Genetic analysis and serum level of cartilage oligomeric matrix protein in patients with pseudoachondroplasia [CMJ: 201082337960590]
BioMed Research International Decreased Plasma COMP and Increased Plasma CTX-II Levels in a Chinese Pseudoachondroplasia Family with Novel COMP Mutation [pubmed:29104872]
Molecular Medicine Reports Mori Folium water extract alleviates articular cartilage damages and inflammatory responses in monosodium iodoacetate?induced osteoarthritis rats [pubmed:29067461]
Archives of Rheumatology Cartilage Oligomeric Matrix Protein Levels in Synovial Fluid in Patients With Primary Knee Osteoarthritis And Healthy Controls: A Preliminary Comparative Analysis With Serum Cartilage Oligomeric Matrix Protein [doi: 10.5606/ArchRheumatol.2017.6220]
Genetic Testing and Molecular Biomarkers Cartilage Oligomeric Matrix Protein Levels in Type 2 Diabetes Associated with Primary Knee Osteoarthritis Patients [Pubmed: 30526057]
International Journal of Clinical and Experimental Medicine The relationship between the serum and synovial fluid osteopontin (OPN), cartilage oligomeric matrix protein (COMP), and the severity of knee osteoarthritis … []
British Journal of Medicine and Medical Research Diagnostic Accuracy of Cartilage Oligomeric Matrix Protein (COMP), for Cartilage Damage in Rheumatoid Arthritis []
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