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促腎上皮質(zhì)激素釋放激素(CRF)檢測試劑盒DIY材料(酶聯(lián)免疫吸附試驗(yàn)法)

ELISA Kit DIY Materials for Corticotropin Releasing Factor (CRF)

CRH; Corticotropin Releasing Hormone; Corticoliberin

  • 促腎上皮質(zhì)激素釋放激素(CRF)檢測試劑盒DIY材料(酶聯(lián)免疫吸附試驗(yàn)法) 產(chǎn)品包裝(模擬)
  • 促腎上皮質(zhì)激素釋放激素(CRF)檢測試劑盒DIY材料(酶聯(lián)免疫吸附試驗(yàn)法) 產(chǎn)品包裝(模擬)
  • Certificate 通過ISO 9001、ISO 13485質(zhì)量體系認(rèn)證

特異性

試劑盒中的抗體對(duì)促腎上皮質(zhì)激素釋放激素(CRH)具有高靈敏度和高特異性,經(jīng)檢測與其它相似物質(zhì)無明顯交叉反應(yīng)。

用法

1.用100μL每孔的工作濃度的包被抗體包被96孔板,4度孵育過夜或者37度孵育2小時(shí);
2.洗板1次;
3.用200μL每孔的工作濃度的封閉液,37度孵育1.5小時(shí);
4.洗板1次。酶標(biāo)板即可用于ELISA實(shí)驗(yàn),后續(xù)使用方法與ELISA同。

儲(chǔ)存

抗體,標(biāo)準(zhǔn)品和HRP標(biāo)記的鏈霉親和素在-20度保存,TMB需在4度保存,96孔板在室溫保存。所有組分保質(zhì)期為1年,試劑一旦開封,在4度保存時(shí)間為1個(gè)月。

配套試劑

相關(guān)產(chǎn)品

編號(hào) 適用物種:Mus musculus (Mouse,小鼠) 應(yīng)用(僅供研究使用,不用于臨床診斷!)
RPA835Mu01 促腎上皮質(zhì)激素釋放激素(CRF)重組蛋白 Positive Control; Immunogen; SDS-PAGE; WB.
RPA835Mu02 促腎上皮質(zhì)激素釋放激素(CRF)重組蛋白 Positive Control; Immunogen; SDS-PAGE; WB.
CPA835Mu21 促腎上皮質(zhì)激素釋放激素(CRF)卵白蛋白偶聯(lián)物 Immunogen; SDS-PAGE; WB.
CPA835Mu22 促腎上皮質(zhì)激素釋放激素(CRF)卵白蛋白偶聯(lián)物 Immunogen; SDS-PAGE; WB.
CPA835Mu33 促腎上皮質(zhì)激素釋放激素(CRF)鑰孔血藍(lán)蛋白偶聯(lián)物 Immunogen; SDS-PAGE; WB.
PAA835Mu09 促腎上皮質(zhì)激素釋放激素(CRF)多克隆抗體 WB; IHC; ICC; IP.
PAA835Mu01 促腎上皮質(zhì)激素釋放激素(CRF)多克隆抗體 WB; IHC; ICC; IP.
PAA835Mu02 促腎上皮質(zhì)激素釋放激素(CRF)多克隆抗體 WB; IHC; ICC; IP.
PAA835Mu07 促腎上皮質(zhì)激素釋放激素(CRF)多克隆抗體 WB; IHC; ICC; IP.
PAA835Mu08 促腎上皮質(zhì)激素釋放激素(CRF)多克隆抗體 WB; IHC; ICC; IP.
LAA835Mu81 促腎上皮質(zhì)激素釋放激素(CRF)多克隆抗體(異硫氰酸熒光素標(biāo)記) WB; IHC; ICC; IF.
LAA835Mu71 促腎上皮質(zhì)激素釋放激素(CRF)多克隆抗體(生物素標(biāo)記) WB; IHC; ICC.
MEA835Mu 促腎上皮質(zhì)激素釋放激素(CRF)檢測試劑盒(酶聯(lián)免疫吸附試驗(yàn)法,小樣本) Enzyme-linked immunosorbent assay for Antigen Detection.
CEA835Mu 促腎上皮質(zhì)激素釋放激素(CRF)檢測試劑盒(酶聯(lián)免疫吸附試驗(yàn)法) Enzyme-linked immunosorbent assay for Antigen Detection.
LMA835Mu 促腎上皮質(zhì)激素釋放激素(CRF)等多因子檢測試劑盒(流式熒光發(fā)光法) FLIA Kit for Antigen Detection.
KSA835Mu11 促腎上皮質(zhì)激素釋放激素(CRF)檢測試劑盒DIY材料(酶聯(lián)免疫吸附試驗(yàn)法) Main materials for "Do It (ELISA Kit) Yourself".

參考文獻(xiàn)

雜志 參考文獻(xiàn)
Toxicology Letters Regulation of corticosterone secretion is modified by PFOS exposure at different levels of the hypothalamic–pituitary–adrenal axis in adult male rats [Pubmed:24440345]
Bipolar?Disord. Immune dysfunction in bipolar disorder and suicide risk: is there an association between peripheral corticotropin‐releasing hormone and interleukin‐1β? [Pubmed:24862833]
Peptides Differential stress response in rats subjected to chronic mild stress is accompanied by changes in CRH-family gene expression at the pituitary level [Pubmed:25236411]
Ann Dermatol Acute?Stress-Induced?Changes?in?Follicular?Dermal?Papilla?Cells?and?Mobilization?of?Mast?Cells:Implications?for?Hair?Growth. [pubmed:27746640]
Neuroscience Heat stress-induced neuroinflammation and aberration in monoamine levels in hypothalamus are associated with temperature dysregulation [pubmed:28663093]
Biomed Research International Colonic Transit Time and Gut Peptides in Adult Patients with Slow and Normal Colonic Transit Constipation [pubmed:29082242]
Biochemical Pharmacology Effects of n-3 PUFA enriched and n-3 PUFA deficient diets in na?ve and Aβ-treated female rats [Pubmed:30028991]
Frontiers in Pharmacology Vaccinium bracteatum Leaf Extract Reverses Chronic Restraint Stress-Induced Depression-Like Behavior in Mice: Regulation of Hypothalamic-Pituitary?… [Pubmed:30038568]
Toxicology Letters Perfluorooctane sulfonate (PFOS) can alter the hypothalamic-pituitary-adrenal (HPA) axis activity by modifying CRF1 and glucocorticoid receptors [Pubmed:29807116]
Neuropsychiatric disease and treatment The depressive-like behaviors of chronic unpredictable mild stress-treated mice, ameliorated by Tibetan medicine Zuotai: involvement in the hypothalamic?… [Pubmed:29379286]
BMC?Veterinary?Research Hypothalamus-pituitary-adrenal axis involves in anti-viral ability through regulation of immune response in piglets infected by highly pathogenic porcine?… [Pubmed:29540178]
Bioscience Research Bioscience Research []
FASEB Journal Contribution of DNA methylation in chronic stress–induced cardiac remodeling and arrhythmias in mice [Pubmed: 31431066]
Aging Cell Altered glucocorticoid metabolism represents a feature of macroph‐aging [Pubmed: 32463582]
Endocr J Impairment of CRH in the intestinal mucosal epithelial barrier of pregnant Bama miniature pig induced by restraint stress [33408312]
CRYOBIOLOGY O-GlcNAc/Akt pathway regulates glucose metabolism and reduces apoptosis in liver of piglets with acute cold stress [33651993]
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