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凋亡相關(guān)因子(FAS)檢測(cè)試劑盒DIY材料(酶聯(lián)免疫吸附試驗(yàn)法)

ELISA Kit DIY Materials for Factor Related Apoptosis (FAS)

CD95; ALPS1A; ALPS1-A; APO1; APT1; FAS1; FASTM; TNFRSF6; Fas Receptor; TNF Receptor Superfamily Member 6; Tumor Necrosis Factor Receptor Superfamily Member 6

  • 凋亡相關(guān)因子(FAS)檢測(cè)試劑盒DIY材料(酶聯(lián)免疫吸附試驗(yàn)法) 產(chǎn)品包裝(模擬)
  • 凋亡相關(guān)因子(FAS)檢測(cè)試劑盒DIY材料(酶聯(lián)免疫吸附試驗(yàn)法) 產(chǎn)品包裝(模擬)
  • Certificate 通過(guò)ISO 9001、ISO 13485質(zhì)量體系認(rèn)證

特異性

試劑盒中的抗體對(duì)凋亡相關(guān)因子(FAS)具有高靈敏度和高特異性，經(jīng)檢測(cè)與其它相似物質(zhì)無(wú)明顯交叉反應(yīng)。

用法

1.用100μL每孔的工作濃度的包被抗體包被96孔板，4度孵育過(guò)夜或者37度孵育2小時(shí)；
2.洗板1次；
3.用200μL每孔的工作濃度的封閉液,37度孵育1.5小時(shí)；
4.洗板1次。酶標(biāo)板即可用于ELISA實(shí)驗(yàn)，后續(xù)使用方法與ELISA同。

儲(chǔ)存

抗體，標(biāo)準(zhǔn)品和HRP標(biāo)記的鏈霉親和素在-20度保存，TMB需在4度保存，96孔板在室溫保存。所有組分保質(zhì)期為1年，試劑一旦開(kāi)封，在4度保存時(shí)間為1個(gè)月。

配套試劑

相關(guān)產(chǎn)品

編號(hào) 適用物種：Mus musculus (Mouse，小鼠) 應(yīng)用(僅供研究使用，不用于臨床診斷！)
EPA030Mu61 凋亡相關(guān)因子(FAS)真核蛋白 Positive Control; Immunogen; SDS-PAGE; WB.
RPA030Mu01 凋亡相關(guān)因子(FAS)重組蛋白 Positive Control; Immunogen; SDS-PAGE; WB.
RPA030Mu02 凋亡相關(guān)因子(FAS)重組蛋白 Positive Control; Immunogen; SDS-PAGE; WB.
PAA030Mu02 凋亡相關(guān)因子(FAS)多克隆抗體 WB; IHC; ICC; IP.
PAA030Mu01 凋亡相關(guān)因子(FAS)多克隆抗體 WB; IHC; ICC; IP.
LAA030Mu71 凋亡相關(guān)因子(FAS)多克隆抗體(生物素標(biāo)記) WB; IHC; ICC.
SEA030Mu 凋亡相關(guān)因子(FAS)檢測(cè)試劑盒(酶聯(lián)免疫吸附試驗(yàn)法) Enzyme-linked immunosorbent assay for Antigen Detection.
SCA030Mu 凋亡相關(guān)因子(FAS)檢測(cè)試劑盒(化學(xué)發(fā)光免疫分析法) Chemiluminescent immunoassay for Antigen Detection.
LMA030Mu 凋亡相關(guān)因子(FAS)等多因子檢測(cè)試劑盒(流式熒光發(fā)光法) FLIA Kit for Antigen Detection.
KSA030Mu01 凋亡相關(guān)因子(FAS)檢測(cè)試劑盒DIY材料(酶聯(lián)免疫吸附試驗(yàn)法) 自己動(dòng)手制作ELISA試劑盒的主要材料

參考文獻(xiàn)

雜志 參考文獻(xiàn)
European Journal of Pharmacology Montelukast abrogates rhabdomyolysis-induced acute renal failure via rectifying detrimental changes in antioxidant profile and systemic cytokines and apoptotic factors production [ScienceDirect: S0014299912002580]
J Sci Food Agric. Beneficial effects of Allium sativum L. stem extract on lipid metabolism and antioxidant status in obese mice fed a high fat diet [Wiley: Source]
Tumor Biology The diplotype Fas? 1377A/? 670G as a genetic marker to predict a lower risk of breast cancer in Chinese women [Springer:Source]
Naunyn Schmiedebergs Arch Pharmacol The multi-kinase inhibitor pazopanib targets hepatic stellate cell activation and apoptosis alleviating progression of liver fibrosis [PubMed: 26269412]
Pharmacological Reports The heat shock protein 90 inhibitor, 18-AAG, attenuates thioacetamide induced liver fibrosis in mice [Science: Article]
PLoS One Aronia melanocarpa?Extract Ameliorates Hepatic Lipid Metabolism through PPARγ2 Downregulation [10.1371]
Molecular Diagnosis & Therapy The Utility of Biomarkers in Osteoporosis Management [pubmed:28271451]
PLoS One Aronia melanocarpa Extract Ameliorates Hepatic Lipid Metabolism through PPARγ2 Downregulation [pubmed:28081181]
Journal of Microbiology, Immunology and Infection Identification of a panel of serum protein markers in early stage of sepsis and its validation in a cohort of patients [pubmed:28655573]
Journal of Functional Foods Polysaccharides from Cyclocarya paliurus: Chemical composition and lipid-lowering effect on rats challenged with high-fat diet [10.1016/j.jff.2017.07.020]
Toxics Zinc Oxide Nanoparticles Induced Oxidative DNA Damage, Inflammation and Apoptosis in Rat's Brain after Oral Exposure [Pubmed:29861430]
Indian?Journal?of?Medical?and?Paediatric?Oncology The correlation between the level of doxorubicin-induced cardiac damage and serum soluble Fas in an experimental rat model [Doi: 10.4103/ijmpo.ijmpo_82_17]
Scientific Reports EGF-mediated reduced miR-92a-1-5p controls HTR-8/SVneo cell invasion through activation of MAPK8 and FAS which in turn increase MMP-2/-9 expression [Pubmed: 32703964]
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