葡萄糖轉運蛋白4(GLUT4)檢測試劑盒DIY材料(酶聯免疫吸附試驗法)
ELISA Kit DIY Materials for Glucose Transporter 4 (GLUT4)
SLC2A4; Solute carrier family 2, facilitated glucose transporter member 4; Glucose transporter type 4, insulin-responsive
- 編號KSC023Hu01
- 物種Homo sapiens (Human,人) 相同的名稱,不同的物種。
- 試劑內容 包被抗體、檢測抗體、標準品、HRP標記的鏈霉親和素、TMB底物、96孔板
- 可檢測樣本Tissue homogenates, cell lysates and other biological fluids.
- 可適用原理雙抗夾心酶聯免疫吸附試驗法測抗原
- 可檢測范圍1.25-20ng/mL
- 可適靈敏度0.51ng/mL
- 應用自己動手制作ELISA試劑盒的主要材料
- 下載 英文說明書 中文說明書
- 規(guī)格 96T*596T*10 96T*20 96T*50 96T*100
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特異性
試劑盒中的抗體對葡萄糖轉運蛋白4(GLUT4)具有高靈敏度和高特異性,經檢測與其它相似物質無明顯交叉反應。
用法
1.用100μL每孔的工作濃度的包被抗體包被96孔板,4度孵育過夜或者37度孵育2小時;
2.洗板1次;
3.用200μL每孔的工作濃度的封閉液,37度孵育1.5小時;
4.洗板1次。酶標板即可用于ELISA實驗,后續(xù)使用方法與ELISA同。
儲存
抗體,標準品和HRP標記的鏈霉親和素在-20度保存,TMB需在4度保存,96孔板在室溫保存。所有組分保質期為1年,試劑一旦開封,在4度保存時間為1個月。
配套試劑
增值服務
相關產品
編號 | 適用物種:Homo sapiens (Human,人) | 應用(僅供研究使用,不用于臨床診斷!) |
RPC023Hu01 | 葡萄糖轉運蛋白4(GLUT4)重組蛋白 | Positive Control; Immunogen; SDS-PAGE; WB. |
PAC023Hu01 | 葡萄糖轉運蛋白4(GLUT4)多克隆抗體 | WB; IHC; ICC; IP. |
MAC023Hu22 | 葡萄糖轉運蛋白4(GLUT4)單克隆抗體 | WB; IHC; ICC; IP. |
SEC023Hu | 葡萄糖轉運蛋白4(GLUT4)檢測試劑盒(酶聯免疫吸附試驗法) | Enzyme-linked immunosorbent assay for Antigen Detection. |
LMC023Hu | 葡萄糖轉運蛋白4(GLUT4)等多因子檢測試劑盒(流式熒光發(fā)光法) | FLIA Kit for Antigen Detection. |
KSC023Hu01 | 葡萄糖轉運蛋白4(GLUT4)檢測試劑盒DIY材料(酶聯免疫吸附試驗法) | 自己動手制作ELISA試劑盒的主要材料 |
參考文獻
雜志 | 參考文獻 |
Polar Record | Glucose transporter-4 in white blood cells of young and old sled dogs: a model for human biomarker development [Cambridge: Source] |
The International Journal of Biochemistry & Cell Biology | Conditioning causes an increase in glucose transporter-4 levels in mononuclear cells in sled dogs [Pubmed:25236492] |
Psychoneuroendocrinology | Maternal stress predicts altered biogenesis and the profile of mitochondrial proteins in the frontal cortex and hippocampus of adult offspring rats [PubMed: 26143539] |
PLOS ONE | Antidiabetic Effect of Galantamine: Novel Effect for a Known Centrally Acting Drug [PubMed: 26262991] |
American Journal of Animal and Veterinary Sciences | Metabolic Features of Heart Failure with Different Etiology [ofsp11075] |
Journal of Pharmaceutical Negative Results | Hibiscus sabdariffa linn fruits methanolic extract and fractions mediated glucose uptake stimulation and glucose transporter 4 regulation [Doi: 10.4103/jpnr.JPNR_14_18] |
eureka herba indonesia | Effect of Noni Fruit Extract (Morinda citrifolia) on Glucose Intake to Diabetes Mellitus White Rat Muscle Tissue [] |
eureka herba indonesia | The Effects of Sambiloto Leaf Extract (Andrographis peniculata) on Blood Sugar Regulation: an In Vivo Study [] |
eureka herba indonesia | Effect of Bay Leaf Extract (Syzygium polyanthum) on Blood Sugar Regulation via GLUT4 Protein Regulation in White Rat Muscle Tissue Induced Aloxan [] |
Eureka Herba Indonesia | Potential Combination of Tinospora crispa, Andrographis paniculata, Cinamomum burmanii, Syzygium polyanthum and Momordica charantia Extracts Against??- [] |
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