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促性腺激素釋放激素(GnRH)檢測試劑盒DIY材料(酶聯(lián)免疫吸附試驗法)

ELISA Kit DIY Materials for Gonadotropin Releasing Hormone (GnRH)

GNRH1; GRH; LNRH; LHRH; LH-RH I; Luliberin; Luteinizing-Hormone Releasing Hormone; Progonadoliberin-1; Gonadoliberin I; Gonadorelin; GnRH-associated peptide 1

  • 促性腺激素釋放激素(GnRH)檢測試劑盒DIY材料(酶聯(lián)免疫吸附試驗法) 產(chǎn)品包裝(模擬)
  • 促性腺激素釋放激素(GnRH)檢測試劑盒DIY材料(酶聯(lián)免疫吸附試驗法) 產(chǎn)品包裝(模擬)
  • Certificate 通過ISO 9001、ISO 13485質(zhì)量體系認證

特異性

試劑盒中的抗體對促性腺激素釋放激素(GnRH)具有高靈敏度和高特異性，經(jīng)檢測與其它相似物質(zhì)無明顯交叉反應(yīng)。

用法

1.用100μL每孔的工作濃度的包被抗體包被96孔板，4度孵育過夜或者37度孵育2小時；
2.洗板1次；
3.用200μL每孔的工作濃度的封閉液,37度孵育1.5小時；
4.洗板1次。酶標板即可用于ELISA實驗，后續(xù)使用方法與ELISA同。

儲存

抗體，標準品和HRP標記的鏈霉親和素在-20度保存，TMB需在4度保存，96孔板在室溫保存。所有組分保質(zhì)期為1年，試劑一旦開封，在4度保存時間為1個月。

配套試劑

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參考文獻

雜志 參考文獻
Mol Pharmacol. Maternal exposure to dioxin imprints sexual immaturity of the pups through fixing the status of the reduced expression of hypothalamic gonadotropin-releasing … [Pubmed:24132183]
Journal of Sports Science & Medicine Heavy Resistance Training and Supplementation With the Alleged Testosterone Booster Nmda has No Effect on Body Composition, Muscle Performance, and Serum Hormones Associated With the Hypothalamo-Pituitary-Gonadal Axis in Resistance-Trained Males [Pubmed:24570624]
Environmental Research Perfluorooctane sulfonate effects on the reproductive axis in adult male rats [Pubmed:25171141]
Toxicology Letters Possible role of serotonin and neuropeptide Y on the disruption of the reproductive axis activity by perfluorooctane sulfonate [Pubmed:25623392]
Toxicol Sci Chronic exposure of female mice to an environmental level of perfluorooctane sulfonate suppresses estrogen synthesis through reduced histone H3K14 acetylation of the StAR promoter leading to deficits in follicular development and ovulation [PubMed: 26358002]
Int J Mol Sci. Overexpression of PRL7D1 in Leydig Cells Causes Male Reproductive Dysfunction in Mice [Pubmed:26771609]
Oral Presentations OP0019-HPR Effectiveness of A Progressive Resistance Strength Program on Hand Osteoarthritis: A Randomised Controlled Trial [60.1.abstract]
Oral Presentations OP0021-HPR Sleep Disorders in Patients with Rheumatoid Arthritis and Relationship with Disease Activity [60.3.abstract]
Toxicological?Sciences Exposure of pregnant mice to perfluorobutanesulfonate causes hypothyroxinemia and developmental abnormalities in female offspring [pubmed:27803384]
Chronobiology of the immune reponse OP0020?The Association of Gonadotropin-Releasing Hormone and Cytokines in Rheumatoid Arthritis [content:75]
Environmental Science & Technology Pyrethroid Insecticide Cypermethrin Accelerates Pubertal Onset in Male Mice via Disrupting Hypothalamic-Pituitary-Gonadal Axis [pubmed:28731686]
Toxicological?Sciences Impact of Perfluorooctane Sulfonate on Reproductive Ability of Female Mice through Suppression of Estrogen Receptor a-Activated Kisspeptin Neurons [10.1093:toxsci]
GENES Nectin-like molecule 2, a necessary sexual maturation regulator, participates in congenital hypogonadotropic hypogonadism [Pubmed: 32535046]
BioMed Research International Pathophysiological Changes in Female Rats with Estrous Cycle Disorder Induced by Long-Term Heat Stress [Pubmed: 32685488]
JOURNAL OF ETHNOPHARMACOLOGY Zihuai recipe alleviates cyclophosphamide-induced diminished ovarian reserve via suppressing PI3K/AKT-mediated apoptosis [33422655]
J Virol Zika virus infection in the ovary induces continuously elevated progesterone level and compromises conception in interferon α/β receptor-deficient mice [34730391]
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