白介素1受體拮抗劑(IL1RA)檢測試劑盒DIY材料(酶聯(lián)免疫吸附試驗法)
ELISA Kit DIY Materials for Interleukin 1 Receptor Antagonist (IL1RA)
IL1RN; IL1-RA; ICIL1RA; IL1ra3; IL1F3; IRAP;IL-1RA; Interleukin-1 Family Member 3; IL1 inhibitor; Anakinra
- 編號KSA223Rb01
- 物種Oryctolagus cuniculus (Rabbit,兔) 相同的名稱,不同的物種。
- 試劑內(nèi)容 包被抗體、檢測抗體、標準品、HRP標記的鏈霉親和素、TMB底物、96孔板
- 可檢測樣本Serum, plasma, tissue homogenates, cell lysates, cell culture supernates and other biological fluids.
- 可適用原理雙抗夾心酶聯(lián)免疫吸附試驗法測抗原
- 可檢測范圍7.81-500pg/mL
- 可適靈敏度3.2pg/mL
- 應用自己動手制作ELISA試劑盒的主要材料
- 下載 英文說明書 中文說明書
- 規(guī)格 96T*596T*10 96T*20 96T*50 96T*100
- 價格 ¥ 7387 ¥ 12312 ¥ 22162 ¥ 43092 ¥ 73872
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特異性
試劑盒中的抗體對白介素1受體拮抗劑(IL1RA)具有高靈敏度和高特異性,經(jīng)檢測與其它相似物質(zhì)無明顯交叉反應。
用法
1.用100μL每孔的工作濃度的包被抗體包被96孔板,4度孵育過夜或者37度孵育2小時;
2.洗板1次;
3.用200μL每孔的工作濃度的封閉液,37度孵育1.5小時;
4.洗板1次。酶標板即可用于ELISA實驗,后續(xù)使用方法與ELISA同。
儲存
抗體,標準品和HRP標記的鏈霉親和素在-20度保存,TMB需在4度保存,96孔板在室溫保存。所有組分保質(zhì)期為1年,試劑一旦開封,在4度保存時間為1個月。
配套試劑
增值服務
相關(guān)產(chǎn)品
編號 | 適用物種:Oryctolagus cuniculus (Rabbit,兔) | 應用(僅供研究使用,不用于臨床診斷!) |
RPA223Rb01 | 白介素1受體拮抗劑(IL1RA)重組蛋白 | Positive Control; Immunogen; SDS-PAGE; WB. |
PAA223Rb51 | 白介素1受體拮抗劑(IL1RA)多克隆抗體 | WB; IHC; ICC; IP. |
MAA223Rb22 | 白介素1受體拮抗劑(IL1RA)單克隆抗體 | WB; IHC; ICC; IP. |
MAA223Rb21 | 白介素1受體拮抗劑(IL1RA)單克隆抗體 | WB; IHC; ICC; IP. |
LAA223Rb82 | 白介素1受體拮抗劑(IL1RA)單克隆抗體(異硫氰酸熒光素標記) | WB; IHC; ICC; IF. |
LAA223Rb72 | 白介素1受體拮抗劑(IL1RA)單克隆抗體(生物素標記) | WB; IHC; ICC. |
SEA223Rb | 白介素1受體拮抗劑(IL1RA)檢測試劑盒(酶聯(lián)免疫吸附試驗法) | Enzyme-linked immunosorbent assay for Antigen Detection. |
LMA223Rb | 白介素1受體拮抗劑(IL1RA)等多因子檢測試劑盒(流式熒光發(fā)光法) | FLIA Kit for Antigen Detection. |
KSA223Rb01 | 白介素1受體拮抗劑(IL1RA)檢測試劑盒DIY材料(酶聯(lián)免疫吸附試驗法) | 自己動手制作ELISA試劑盒的主要材料 |
參考文獻
雜志 | 參考文獻 |
The Veterinary Journal | Mucosal imbalance of interleukin-1β and interleukin-1 receptor antagonist in canine inflammatory bowel disease [ScienceDirect: S1090023312000962] |
Daily quercetin supplementation over a period of two weeks results in a moderate accumulation of total plasma flavonols in horses [Researchgate:Source] | |
Journal of Equine Veterinary Science | Synovial Fluid and Serum Concentrations of Interleukin-1 Receptor Antagonist and Interleukin-1 [Science: Article] |
Journal of Equine Veterinary Science | A Two-Week Quercetin Supplementation in Horses Results in Moderate Accumulation of Plasma Flavonol Concentrations [Science: Article] |
Scientific publications in peer reviewed journals | Evidence-based review of efficacy and adverse effects of joint medication and evaluation of synovial fluid and serum markers for osteoarthritis in the horse [receive:101658] |
Multiple Sclerosis and Related Disorders | Interleukin 1 receptor antagonist and 2′-5′-oligoadenylate synthetase-like molecules as novel biomarkers for multiple sclerosis patients in Bahrain [pubmed:29141788] |
Advances in Medical Sciences | A similar pro/anti-inflammatory cytokine balance is present in the airways of competitive athletes and non-exercising asthmatics [pubmed:28822267] |
Archives of Medical Research | Winter ambient training conditions are associated with increased bronchial hyperreactivity and with shifts in serum innate immunity proteins in young?… [Pubmed:29379533] |
Journal of Veterinary?Internal?Medicine | Effect of interleukin‐1β on occludin mRNA expression in the duodenal and colonic mucosa of dogs with inflammatory bowel disease [Pubmed:29572935] |
Frontiers in Pharmacology | M10, a Myricetin-3-ObD-Lactose Sodium Salt, Prevents Ulcerative Colitis Through Inhibiting Necroptosis in Mice [Pubmed: 33041798] |
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