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吻素1(KISS1)檢測試劑盒DIY材料(酶聯(lián)免疫吸附試驗法)

ELISA Kit DIY Materials for Kisspeptin 1 (KISS1)

Metastin; KiSS-1 Metastasis-Suppressor

  • 吻素1(KISS1)檢測試劑盒DIY材料(酶聯(lián)免疫吸附試驗法) 產(chǎn)品包裝(模擬)
  • 吻素1(KISS1)檢測試劑盒DIY材料(酶聯(lián)免疫吸附試驗法) 產(chǎn)品包裝(模擬)
  • Certificate 通過ISO 9001、ISO 13485質(zhì)量體系認證

特異性

試劑盒中的抗體對吻素1(KISS1)具有高靈敏度和高特異性,經(jīng)檢測與其它相似物質(zhì)無明顯交叉反應。

用法

1.用100μL每孔的工作濃度的包被抗體包被96孔板,4度孵育過夜或者37度孵育2小時;
2.洗板1次;
3.用200μL每孔的工作濃度的封閉液,37度孵育1.5小時;
4.洗板1次。酶標板即可用于ELISA實驗,后續(xù)使用方法與ELISA同。

儲存

抗體,標準品和HRP標記的鏈霉親和素在-20度保存,TMB需在4度保存,96孔板在室溫保存。所有組分保質(zhì)期為1年,試劑一旦開封,在4度保存時間為1個月。

配套試劑

相關產(chǎn)品

編號 適用物種:Homo sapiens (Human,人) 應用(僅供研究使用,不用于臨床診斷!)
RPC559Hu01 吻素1(KISS1)重組蛋白 Positive Control; Immunogen; SDS-PAGE; WB.
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CEC559Hu 吻素1(KISS1)檢測試劑盒(酶聯(lián)免疫吸附試驗法) Enzyme-linked immunosorbent assay for Antigen Detection.
LMC559Hu 吻素1(KISS1)等多因子檢測試劑盒(流式熒光發(fā)光法) FLIA Kit for Antigen Detection.
KSC559Hu01 吻素1(KISS1)檢測試劑盒DIY材料(酶聯(lián)免疫吸附試驗法) Main materials for "Do It (ELISA Kit) Yourself".

參考文獻

雜志 參考文獻
Mol Endocrinol. LIM-homeodomain transcription factor Isl-1 mediates kisspeptin's effect on insulin secretion in mice. [Pubmed:24956377]
臨床兒科雜志 雙酚A 與女童性早熟的關系 [Fileup: Pdf]
J Pediatr Endocrinol Metab The relation of urinary bisphenol A with kisspeptin in girls diagnosed with central precocious puberty and premature thelarche [PubMed: 26565542]
Archives of Physiology and Biochemistry Circulating levels of leptin, nesfatin-1 and kisspeptin in postmenopausal obese women [Pubmed:27011110]
International Journal of Endocrinology The Changes of Serum Leptin and Kisspeptin Levels in Chinese Children and Adolescents in Different Pubertal Stages [pubmed:27990162]
Journal of?Clinical?Research?in Pediatric Endocrinology Basal Serum Neurokinin B Levels in Differentiating Idiopathic Central Precocious Puberty from Premature Thelarche. [pubmed:28008860]
BioMed Research International Kisspeptin Protein in Seminal Plasma Is Positively Associated with Semen Quality: Results from the MARHCS Study in Chongqing, China []
OBESITY?SURGERY Lack of Improvement of Sperm Characteristics in Obese Males After Obesity Surgery Despite the Beneficial Changes Observed in Reproductive Hormones [Pubmed: 30806913]
International Journal of Endocrinology Serum Kisspeptin and Its Relation to Metabolic Parameters and Glucose Metabolism in Prepubertal and Pubertal Obese Children [Pubmed: 33224197]
journal of reproduction and fertility KISS1R Signaling Modulates Gonadotropin Sensitivity in Mouse Leydig Cell [Pubmed: 33112765]
Beyond GnRH, LH and FSH: the role of kisspeptin on hypothalalmic-3 pituitary gonadal (HPG) axis pathology and diagnostic consideration 4 []
Medeni Med J Kisspeptin and Hematologic Parameters as Predictive Biomarkers for First-Trimester Abortions [34239761]
Arch Gynecol Obstet Comparison of the Kisspeptin levels in early onset preeclampsia and late-onset preeclampsia [Pubmed:35039882]
J Pediatr Endocrinol Metab Serum kisspeptin, leptin, neuropeptide Y, and neurokinin B levels in adolescents with polycystic ovary syndrome [Pubmed:35170267]
Genes Differential Expression of Kisspeptin System and Kisspeptin Receptor Trafficking during Spermatozoa Transit in the Epididymis [Pubmed:35205340]
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