脂蛋白脂酶(LPL)檢測試劑盒DIY材料(酶聯(lián)免疫吸附試驗法)
ELISA Kit DIY Materials for Lipoprotein lipase (LPL)
LIPD; Lipase, Lipoprotein
- 編號KSA386Ra01
- 物種Rattus norvegicus (Rat,大鼠) 相同的名稱,不同的物種。
- 試劑內容 包被抗體、檢測抗體、標準品、HRP標記的鏈霉親和素、TMB底物、96孔板
- 可檢測樣本Plasma.
- 可適用原理雙抗夾心酶聯(lián)免疫吸附試驗法測抗原
- 可檢測范圍31.25-2000pg/mL
- 可適靈敏度13.6pg/mL
- 應用自己動手制作ELISA試劑盒的主要材料
- 下載 英文說明書 中文說明書
- 規(guī)格 96T*596T*10 96T*20 96T*50 96T*100
- 價格 ¥ 7387 ¥ 12312 ¥ 22162 ¥ 43092 ¥ 73872
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特異性
試劑盒中的抗體對脂蛋白脂酶(LIPD)具有高靈敏度和高特異性,經(jīng)檢測與其它相似物質無明顯交叉反應。
用法
1.用100μL每孔的工作濃度的包被抗體包被96孔板,4度孵育過夜或者37度孵育2小時;
2.洗板1次;
3.用200μL每孔的工作濃度的封閉液,37度孵育1.5小時;
4.洗板1次。酶標板即可用于ELISA實驗,后續(xù)使用方法與ELISA同。
儲存
抗體,標準品和HRP標記的鏈霉親和素在-20度保存,TMB需在4度保存,96孔板在室溫保存。所有組分保質期為1年,試劑一旦開封,在4度保存時間為1個月。
配套試劑
增值服務
相關產(chǎn)品
編號 | 適用物種:Rattus norvegicus (Rat,大鼠) | 應用(僅供研究使用,不用于臨床診斷!) |
RPA386Ra01 | 脂蛋白脂酶(LPL)重組蛋白 | Positive Control; Immunogen; SDS-PAGE; WB. |
PAA386Ra01 | 脂蛋白脂酶(LPL)多克隆抗體 | WB; IHC; ICC; IP. |
MAA386Ra21 | 脂蛋白脂酶(LPL)單克隆抗體 | WB; IHC; ICC; IP. |
SEA386Ra | 脂蛋白脂酶(LPL)檢測試劑盒(酶聯(lián)免疫吸附試驗法) | Enzyme-linked immunosorbent assay for Antigen Detection. |
LMA386Ra | 脂蛋白脂酶(LPL)等多因子檢測試劑盒(流式熒光發(fā)光法) | FLIA Kit for Antigen Detection. |
KSA386Ra01 | 脂蛋白脂酶(LPL)檢測試劑盒DIY材料(酶聯(lián)免疫吸附試驗法) | 自己動手制作ELISA試劑盒的主要材料 |
PGA386Ra01 | 脂蛋白脂酶(LPL)引物對 | PCR |
參考文獻
雜志 | 參考文獻 |
Diabetes | Severity of Diabetes Governs Vascular Lipoprotein Lipase by Affecting Enzyme Dimerization and Disassembly [PubMed: 21646389] |
The?Korean Journal of?Internal Medicine | Clinical efficacy of serum lipase subtype analy-sis for the differential diagnosis of pancreatic and non-pancreatic lipase elevation [Pubmed:27243230] |
The Journal of Lipid Research | ReCavia (Guinea pig )lation of Plasma Lipid Homeostasis by Hepatic Lipoprotein Lipase in Adult Mice [Pubmed:27234787] |
Journal of Functional Foods | Polysaccharides from Cyclocarya paliurus: Chemical composition and lipid-lowering effect on rats challenged with high-fat diet [10.1016/j.jff.2017.07.020] |
Biotechnology Progress | Mass Spectrometric Evaluation of Upstream and Downstream Process Influences on Host Cell Protein Patterns in Biopharmaceutical Products [Pubmed: 30767403] |
arteriosclerosis thrombosis and vascular biology | Integrin β3 Deficiency Results in Hypertriglyceridemia Via Disrupting LPL (Lipoprotein Lipase) Secretion [Pubmed: 32237906] |
Clinical Nutrition ESPEN | Abdominal fat distribution modulates the metabolic effects of exogenous ketones in individuals with new-onset prediabetes after acute pancreatitis: Results from a randomized placebo-controlled trial [34024503] |
Antioxidants | Bio-Evaluation of the Wound Healing Activity of Artemisia judaica L. as Part of the Plant's Use in Traditional Medicine; Phytochemical, Antioxidant, Anti-Inflammatory?… [Pubmed:35204215] |
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