蛋白激酶Cβ1(PKCb1)檢測試劑盒DIY材料(酶聯(lián)免疫吸附試驗法)
ELISA Kit DIY Materials for Protein Kinase C Beta 1 (PKCb1)
PKC-B1; PRKCB; PRKCB1
- 編號KSB045Rb01
- 物種Oryctolagus cuniculus (Rabbit,兔) 相同的名稱,不同的物種。
- 試劑內(nèi)容 包被抗體、檢測抗體、標(biāo)準(zhǔn)品、HRP標(biāo)記的鏈霉親和素、TMB底物、96孔板
- 可檢測樣本Serum, plasma, tissue homogenates, cell lysates and other biological fluids.
- 可適用原理雙抗夾心酶聯(lián)免疫吸附試驗法測抗原
- 可檢測范圍0.156-10ng/mL
- 可適靈敏度0.061ng/mL
- 應(yīng)用自己動手制作ELISA試劑盒的主要材料
- 下載 英文說明書 中文說明書
- 規(guī)格 96T*596T*10 96T*20 96T*50 96T*100
- 價格 ¥ 7445 ¥ 12408 ¥ 22334 ¥ 43428 ¥ 74448
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特異性
試劑盒中的抗體對蛋白激酶Cβ1(PKCb1)具有高靈敏度和高特異性,經(jīng)檢測與其它相似物質(zhì)無明顯交叉反應(yīng)。
用法
1.用100μL每孔的工作濃度的包被抗體包被96孔板,4度孵育過夜或者37度孵育2小時;
2.洗板1次;
3.用200μL每孔的工作濃度的封閉液,37度孵育1.5小時;
4.洗板1次。酶標(biāo)板即可用于ELISA實驗,后續(xù)使用方法與ELISA同。
儲存
抗體,標(biāo)準(zhǔn)品和HRP標(biāo)記的鏈霉親和素在-20度保存,TMB需在4度保存,96孔板在室溫保存。所有組分保質(zhì)期為1年,試劑一旦開封,在4度保存時間為1個月。
配套試劑
增值服務(wù)
相關(guān)產(chǎn)品
編號 | 適用物種:Oryctolagus cuniculus (Rabbit,兔) | 應(yīng)用(僅供研究使用,不用于臨床診斷!) |
RPB045Rb01 | 蛋白激酶Cβ1(PKCb1)重組蛋白 | Positive Control; Immunogen; SDS-PAGE; WB. |
PAB045Rb51 | 蛋白激酶Cβ1(PKCb1)多克隆抗體 | WB; IHC; ICC; IP. |
PAB045Rb01 | 蛋白激酶Cβ1(PKCb1)多克隆抗體 | WB; IHC; ICC; IP. |
LAB045Rb71 | 蛋白激酶Cβ1(PKCb1)多克隆抗體(生物素標(biāo)記) | WB; IHC; ICC. |
KSB045Rb01 | 蛋白激酶Cβ1(PKCb1)檢測試劑盒DIY材料(酶聯(lián)免疫吸附試驗法) | 自己動手制作ELISA試劑盒的主要材料 |
參考文獻(xiàn)
雜志 | 參考文獻(xiàn) |
Vascular Pharmacology | Hesperetin ameliorates hyperglycemia induced retinal vasculopathy via anti-angiogenic effects in experimental diabetic rats [ScienceDirect: S1537189112000407] |
Food and Chemical Toxicology | Prevention and management of diabetic retinopathy in STZ diabetic rats by Tinospora cordifolia and its molecular mechanisms [PubMed: S0278691512004103] |
Journal of ocular pharmacology and therapeutics | Retinoprotective Effects of Moringa oleifera via Antioxidant, Anti-Inflammatory, and Anti-Angiogenic Mechanisms in Streptozotocin-Induced Diabetic Rats [Pubmed: 23215831] |
Mol Cell Biochem. | Effects of Trigonella foenum-graecum (L.) on retinal oxidative stress, and proinflammatory and angiogenic molecular biomarkers in streptozotocin-induced diabetic rats [Pubmed:24242137] |
J Clin Diagn Res. | Evaluation of Protein Kinase Cβ and PPARγ Activity in Diabetic Rats Supplemented with Momordica charantia [pmc:PMC4866090] |
Journal of Clinical&Diagnostic Research | Evaluation?of?Protein?Kinase?Cβ and PPARγ?Activity?in?Diabetic?RatsSupplemented?with?Momordica?charantia. [pubmed:27190792] |
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