S100鈣結(jié)合蛋白A7(S100A7)檢測(cè)試劑盒DIY材料(酶聯(lián)免疫吸附試驗(yàn)法)
ELISA Kit DIY Materials for S100 Calcium Binding Protein A7 (S100A7)
S100-A7; PSOR1; S100A7c; Psoriasin 1
- 編號(hào)KSC035Bo01
- 物種Bos taurus; Bovine (Cattle,牛) 相同的名稱,不同的物種。
- 試劑內(nèi)容 包被抗體、檢測(cè)抗體、標(biāo)準(zhǔn)品、HRP標(biāo)記的鏈霉親和素、TMB底物、96孔板
- 可檢測(cè)樣本Serum, plasma, tissue homogenates, cell lysates, cell culture supernates and other biological fluid.
- 可適用原理雙抗夾心酶聯(lián)免疫吸附試驗(yàn)法測(cè)抗原
- 可檢測(cè)范圍0.156-10ng/mL
- 可適靈敏度0.070ng/mL
- 應(yīng)用Main materials for "Do It(ELISA Kit) Yourself"
- 下載 英文說(shuō)明書(shū) 中文說(shuō)明書(shū)
- 規(guī)格 96T*596T*10 96T*20 96T*50 96T*100
- 價(jià)格 ¥ 8561 ¥ 14268 ¥ 25682 ¥ 49938 ¥ 85608
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特異性
試劑盒中的抗體對(duì)S100鈣結(jié)合蛋白A7(S100A7)具有高靈敏度和高特異性,經(jīng)檢測(cè)與其它相似物質(zhì)無(wú)明顯交叉反應(yīng)。
用法
1.用100μL每孔的工作濃度的包被抗體包被96孔板,4度孵育過(guò)夜或者37度孵育2小時(shí);
2.洗板1次;
3.用200μL每孔的工作濃度的封閉液,37度孵育1.5小時(shí);
4.洗板1次。酶標(biāo)板即可用于ELISA實(shí)驗(yàn),后續(xù)使用方法與ELISA同。
儲(chǔ)存
抗體,標(biāo)準(zhǔn)品和HRP標(biāo)記的鏈霉親和素在-20度保存,TMB需在4度保存,96孔板在室溫保存。所有組分保質(zhì)期為1年,試劑一旦開(kāi)封,在4度保存時(shí)間為1個(gè)月。
配套試劑
贈(zèng)品
增值服務(wù)
相關(guān)產(chǎn)品
編號(hào) | 適用物種:Bos taurus; Bovine (Cattle,牛) | 應(yīng)用(僅供研究使用,不用于臨床診斷!) |
RPC035Bo02 | S100鈣結(jié)合蛋白A7(S100A7)重組蛋白 | Positive Control; Immunogen; SDS-PAGE; WB. |
APC035Bo61 | S100鈣結(jié)合蛋白A7(S100A7)活性蛋白 | Cell?culture;?Activity?Assays. |
RPC035Bo01 | S100鈣結(jié)合蛋白A7(S100A7)重組蛋白 | Positive Control; Immunogen; SDS-PAGE; WB. |
EPC035Bo61 | S100鈣結(jié)合蛋白A7(S100A7)真核蛋白 | Positive Control; Immunogen; SDS-PAGE; WB. |
PAC035Bo02 | S100鈣結(jié)合蛋白A7(S100A7)多克隆抗體 | WB |
PAC035Bo01 | S100鈣結(jié)合蛋白A7(S100A7)多克隆抗體 | WB; IHC; ICC; IP. |
SEC035Bo | S100鈣結(jié)合蛋白A7(S100A7)檢測(cè)試劑盒(酶聯(lián)免疫吸附試驗(yàn)法) | Enzyme-linked immunosorbent assay for Antigen Detection. |
KSC035Bo01 | S100鈣結(jié)合蛋白A7(S100A7)檢測(cè)試劑盒DIY材料(酶聯(lián)免疫吸附試驗(yàn)法) | Main materials for "Do It(ELISA Kit) Yourself" |
參考文獻(xiàn)
雜志 | 參考文獻(xiàn) |
Archives of Dermatological Research | Serum levels of antimicrobial peptides and proteins do not correlate with psoriasis severity and are increased after treatment with fumaric acid esters [SpringerLink: t07u24t43r357l15] |
J Proteome Res | Proteomic Signature of Endothelial Dysfunction Identified in the Serum of Acute Ischemic Stroke Patients by the iTRAQ-Based LC–MS Approach [PubMed: 25807139] |
Exp Dermatol | S100A7 (psoriasin) inhibits human epidermal differentiation by enhanced IL-6 secretion through IκB/NF-κB signaling [Pubmed:27060579] |
Experimental Dermatology | S100A7 (psoriasin) inhibits human epidermal differentiation by enhanced IL‐6 secretion through IκB/NF‐κB signalling [pubmed:27060579] |
Experiental And Therapeutic Medicine | Plasma proteins as potential targets of abnormal Savda syndrome in asthma patients treated with unique Uighur prescription [pubmed:28672924] |
Scientific Reports | The psoriasis-associated IL-17A induces and cooperates with IL-36 cytokines to control keratinocyte differentiation and function. [pubmed:29142248] |
Journal of the European Academy of Dermatology and Venereology | A potential contribution of psoriasin to vascular and epithelial abnormalities and inflammation in systemic sclerosis [Pubmed:28681537] |
Scientific Reports | Measurements of AMPs in stratum corneum of atopic dermatitis and healthy skin–tape stripping technique [Pubmed:29374283] |
Neuroscience?Bulletin | Comprehensive proteomic profiling of patients' tears identifies potential biomarkers for the traumatic vegetative state [Pubmed:30019218] |
Journal of Proteomics | Salivary proteins from dysplastic leukoplakia and oral squamous cell carcinoma and their potential for early detection [Pubmed: 31706945] |
Mediators of Inflammation | Alarmins HMGB1, IL-33, S100A7, and S100A12 in Psoriasis Vulgaris [] |
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