分泌型卷曲相關(guān)蛋白1(SFRP1)檢測試劑盒DIY材料(酶聯(lián)免疫吸附試驗法)
ELISA Kit DIY Materials for Secreted Frizzled Related Protein 1 (SFRP1)
FRP1; FRP; FRP-1; FrzA; SARP2; Secreted apoptosis-related protein 2
- 編號KSF880Mu01
- 物種Mus musculus (Mouse，小鼠) 相同的名稱，不同的物種。
- 試劑內(nèi)容 包被抗體、檢測抗體、標準品、HRP標記的鏈霉親和素、TMB底物、96孔板
- 可檢測樣本Serum, plasma, tissue homogenates and other biological fluids.
- 可適用原理雙抗夾心酶聯(lián)免疫吸附試驗法測抗原
- 可檢測范圍31.2-2000pg/mL
- 可適靈敏度12.7pg/mL
- 應(yīng)用自己動手制作ELISA試劑盒的主要材料
- 下載 英文說明書 中文說明書
- 規(guī)格 96T*596T*10 96T*20 96T*50 96T*100
- 價格 ¥ 7776 ¥ 12960 ¥ 23328 ¥ 45360 ¥ 77760
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產(chǎn)品包裝(模擬)
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產(chǎn)品包裝(模擬)
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通過ISO 9001、ISO 13485質(zhì)量體系認證
特異性
試劑盒中的抗體對分泌型卷曲相關(guān)蛋白1(SFRP1)具有高靈敏度和高特異性，經(jīng)檢測與其它相似物質(zhì)無明顯交叉反應(yīng)。
用法
1.用100μL每孔的工作濃度的包被抗體包被96孔板，4度孵育過夜或者37度孵育2小時；
2.洗板1次；
3.用200μL每孔的工作濃度的封閉液,37度孵育1.5小時；
4.洗板1次。酶標板即可用于ELISA實驗，后續(xù)使用方法與ELISA同。
儲存
抗體，標準品和HRP標記的鏈霉親和素在-20度保存，TMB需在4度保存，96孔板在室溫保存。所有組分保質(zhì)期為1年，試劑一旦開封，在4度保存時間為1個月。
配套試劑
增值服務(wù)
相關(guān)產(chǎn)品
| 編號 | 適用物種：Mus musculus (Mouse，小鼠) | 應(yīng)用(僅供研究使用，不用于臨床診斷！) |
| RPF880Mu02 | 分泌型卷曲相關(guān)蛋白1(SFRP1)重組蛋白 | Positive Control; Immunogen; SDS-PAGE; WB. |
| RPF880Mu01 | 分泌型卷曲相關(guān)蛋白1(SFRP1)重組蛋白 | Positive Control; Immunogen; SDS-PAGE; WB. |
| PAF880Mu01 | 分泌型卷曲相關(guān)蛋白1(SFRP1)多克隆抗體 | WB; IHC; ICC; IP. |
| PAF880Mu02 | 分泌型卷曲相關(guān)蛋白1(SFRP1)多克隆抗體 | WB; IHC; ICC; IP. |
| LAF880Mu71 | 分泌型卷曲相關(guān)蛋白1(SFRP1)多克隆抗體(生物素標記) | WB; IHC; ICC. |
| LAF880Mu81 | 分泌型卷曲相關(guān)蛋白1(SFRP1)多克隆抗體(異硫氰酸熒光素標記) | WB; IHC; ICC; IF. |
| SEF880Mu | 分泌型卷曲相關(guān)蛋白1(SFRP1)檢測試劑盒(酶聯(lián)免疫吸附試驗法) | Enzyme-linked immunosorbent assay for Antigen Detection. |
| LMF880Mu | 分泌型卷曲相關(guān)蛋白1(SFRP1)等多因子檢測試劑盒(流式熒光發(fā)光法) | FLIA Kit for Antigen Detection. |
| KSF880Mu01 | 分泌型卷曲相關(guān)蛋白1(SFRP1)檢測試劑盒DIY材料(酶聯(lián)免疫吸附試驗法) | 自己動手制作ELISA試劑盒的主要材料 |
參考文獻
| 雜志 | 參考文獻 |
| BMC Musculoskeletal Disorders | Biomarkers and cytokines of bone turnover: extensive evaluation in a cohort of patients with ankylosing spondylitis [PubMed: 23025387] |
| Exp Cell Res | Wnt Inhibition is Dysregulated in Gliomas and its Re-Establishment Inhibits Proliferation and Tumor Sphere Formation [PubMed: 26712519] |
| BMC Musculoskeletal Disorders | Correlation?of?blood?bone?turnover?biomarkers?and?Wnt?signaling?antagonists?with AS,?DISH,OPLL, and?OYL. [pubmed:28153008] |
| PloS one | Effects of exercise training on circulating levels of Dickkpof-1 and secreted frizzled-related protein-1 in breast cancer survivors: A pilot single-blind randomized controlled trial. [pubmed:28178355] |
| PLoS One | Effects of exercise training on circulating levels of Dickkpof-1 and secreted frizzled-related protein-1 in breast cancer survivors: A pilot single-blind randomized controlled trial [10.1371] |
| Journal of Experimental & Clinical Cancer Research | PHF21B overexpression promotes cancer stem cell-like traits in prostate cancer cells by activating the Wnt/β-catenin signaling pathway [pubmed:28645312] |
| JBMR Plus | Low serum levels of DKK2 predict incident low‐impact fracture in older women [Doi: 10.1002/jbm4.10179] |
| Nature Communications | Immunological history governs human stem cell memory CD4 heterogeneity via the Wnt signaling pathway [Pubmed: 32041953] |
| acta pharmacologica sinica | sFRP1 protects H9c2 cardiac myoblasts from doxorubicin-induced apoptosis by inhibiting the Wnt/PCP-JNK pathway [Pubmed: 32238888] |
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