血管內皮生長因子受體2(VEGFR2)檢測試劑盒DIY材料(酶聯免疫吸附試驗法)
ELISA Kit DIY Materials for Vascular Endothelial Growth Factor Receptor 2 (VEGFR2)
CD309; FLK1; VEGFR; KDR; A Type III Receptor Tyrosine Kinase; Kinase Insert Domain Receptor; Kinase Insert Domain Receptor; Fetal Liver Kinase-1
- 編號KSB367Hu01
- 物種Homo sapiens (Human,人) 相同的名稱,不同的物種。
- 試劑內容 包被抗體、檢測抗體、標準品、HRP標記的鏈霉親和素、TMB底物、96孔板
- 可檢測樣本Serum, plasma, tissue homogenates and other biological fluids.
- 可適用原理雙抗夾心酶聯免疫吸附試驗法測抗原
- 可檢測范圍0.625-40ng/mL
- 可適靈敏度0.236ng/mL
- 應用自己動手制作ELISA試劑盒的主要材料
- 下載 英文說明書 中文說明書
- 規(guī)格 96T*596T*10 96T*20 96T*50 96T*100
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特異性
試劑盒中的抗體對血管內皮生長因子受體2(VEGFR2)具有高靈敏度和高特異性,經檢測與其它相似物質無明顯交叉反應。
用法
1.用100μL每孔的工作濃度的包被抗體包被96孔板,4度孵育過夜或者37度孵育2小時;
2.洗板1次;
3.用200μL每孔的工作濃度的封閉液,37度孵育1.5小時;
4.洗板1次。酶標板即可用于ELISA實驗,后續(xù)使用方法與ELISA同。
儲存
抗體,標準品和HRP標記的鏈霉親和素在-20度保存,TMB需在4度保存,96孔板在室溫保存。所有組分保質期為1年,試劑一旦開封,在4度保存時間為1個月。
配套試劑
增值服務
相關產品
編號 | 適用物種:Homo sapiens (Human,人) | 應用(僅供研究使用,不用于臨床診斷!) |
APB367Hu61 | 血管內皮生長因子受體2(VEGFR2)活性蛋白 | Cell?culture;?Activity?Assays. |
EPB367Hu61 | 血管內皮生長因子受體2(VEGFR2)真核蛋白 | Positive Control; Immunogen; SDS-PAGE; WB. |
APB367Hu01 | 血管內皮生長因子受體2(VEGFR2)活性蛋白 | Cell?culture;?Activity?Assays. |
RPB367Hu01 | 血管內皮生長因子受體2(VEGFR2)重組蛋白 | Positive Control; Immunogen; SDS-PAGE; WB. |
PAB367Hu01 | 血管內皮生長因子受體2(VEGFR2)多克隆抗體 | WB; IHC; ICC; IP. |
LAB367Hu81 | 血管內皮生長因子受體2(VEGFR2)多克隆抗體(異硫氰酸熒光素標記) | WB; IHC; ICC; IF. |
LAB367Hu71 | 血管內皮生長因子受體2(VEGFR2)多克隆抗體(生物素標記) | WB; IHC; ICC. |
FAB367Hu51 | 抗血管內皮生長因子受體2(VEGFR2)多克隆抗體(別藻藍蛋白標記) | FCM |
MAB367Hu23 | 血管內皮生長因子受體2(VEGFR2)單克隆抗體 | WB; IHC; ICC; IP. |
MAB367Hu26 | 血管內皮生長因子受體2(VEGFR2)單克隆抗體 | WB; IHC; ICC; IP. |
RAB367Hu21 | 血管內皮生長因子受體2(VEGFR2)重組抗體 | WB; IF; ICC; IHC; IP; FCM. |
MAB367Hu22 | 血管內皮生長因子受體2(VEGFR2)單克隆抗體 | WB; IHC; ICC; IP. |
MAB367Hu24 | 血管內皮生長因子受體2(VEGFR2)單克隆抗體 | WB; IHC; ICC; IP. |
MAB367Hu21 | 血管內皮生長因子受體2(VEGFR2)單克隆抗體 | WB; IHC; ICC; IP. |
FAB367Hu32 | 抗血管內皮生長因子受體2(VEGFR2)單克隆抗體(別藻藍蛋白/花青染料cy7標記) | FCM |
FAB367Hu82 | 抗血管內皮生長因子受體2(VEGFR2)單克隆抗體(異硫氰酸熒光素標記) | Flow cytometry. |
HEB367Hu | 血管內皮生長因子受體2(VEGFR2)檢測試劑盒(酶聯免疫吸附試驗法,高敏型) | Enzyme-linked immunosorbent assay for Antigen Detection. |
SEB367Hu | 血管內皮生長因子受體2(VEGFR2)檢測試劑盒(酶聯免疫吸附試驗法) | Enzyme-linked immunosorbent assay for Antigen Detection. |
LMB367Hu | 血管內皮生長因子受體2(VEGFR2)等多因子檢測試劑盒(流式熒光發(fā)光法) | FLIA Kit for Antigen Detection. |
KSB367Hu01 | 血管內皮生長因子受體2(VEGFR2)檢測試劑盒DIY材料(酶聯免疫吸附試驗法) | 自己動手制作ELISA試劑盒的主要材料 |
參考文獻
雜志 | 參考文獻 |
APMIS | Peritumoral brain edema in angiomatous supratentorial meningiomas: an investigation of the vascular endothelial growth factor A pathway [PubMed: 23398358] |
Acta Biomater | Antioxidant and bone repair properties of quercetin-functionalized hydroxyapatite: an in vitro osteoblast-osteoclast-endothelial cell co-culture study [PubMed: 26689470] |
Acta Biomaterialia | Antioxidant and bone repair properties of quercetin-functionalized hydroxyapatite: An in vitro osteoblast–osteoclast–endothelial cell co-culture study [Pubmed:26689470] |
Journal?of?Cellular?Physiology | A?Human?3D?In?Vitro?Model?to?Assess?the?Relationship?Between?Osteoporosis?and?Disseminationto?Bone?of?Breast?Cancer?Tumor?Cells. [pubmed:27925188] |
Regenerative Biomaterials | The directional migration and differentiation of mesenchymal stem cells toward vascular endothelial cells stimulated by biphasic calcium phosphate ceramic [10.1093/rb/rbx028] |
International Journal of Immunopathology and Pharmacology | VEGF-R2 and TNF-R1 expression and cytokine production by samples of mammary adenocarcinomas and correlations with histopathological parameters of these?… [Pubmed:29985074] |
JOURNAL OF DERMATOLOGICAL TREATMENT | Effect of isotretinoin (13-cis-retinoic acid) on levels of soluble VEGF receptors (sVEGFR1, sVEGFR2, sVEGFR3) in patients with acne vulgaris [Pubmed: 32043381] |
Journal of Genetic Engineering and Biotechnology | Encapsulated VEGF 121-PLA microparticles promote angiogenesis in human endometrium stromal cells [33523322] |
Mol Cell Biochem | Intuitive repositioning of an anti-depressant drug in combination with tivozanib: precision medicine for breast cancer therapy [34324118] |
BMC Nephrol | Effect of subacute poisoning with lambdacyhalothrin on vascular endothelial growth factor 2 receptor in mice kidneys [Pubmed:35643467] |
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