白蛋白(ALB)檢測試劑盒(酶聯(lián)免疫吸附試驗法)
ELISA Kit for Albumin (ALB)
Serum albumin
- 編號CEB028Bo
- 物種Bos taurus; Bovine (Cattle，牛) 相同的名稱，不同的物種。
- 實驗方法競爭抑制
- 反應時長2h
- 檢測范圍370.4-30,000ng/mL
- 靈敏度最小可檢測劑量小于等于161.3ng/mL.
- 樣本類型serum, plasma, tissue homogenates, cell lysates, cell culture supernates and other biological fluids
- 下載 英文說明書 中文說明書
- 規(guī)格 48T96T 96T*5 96T*10 96T*100
- 價格 ¥ 3352 ¥ 4788 ¥ 21546 ¥ 40698 ¥ 335160
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產(chǎn)品包裝(模擬)
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產(chǎn)品包裝(模擬)
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實驗結果圖
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標準曲線圖
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通過ISO 9001、ISO 13485質量體系認證
特異性
本試劑盒用于檢測白蛋白(ALB)，經(jīng)檢測與其它相似物質無明顯交叉反應。
由于受到技術及樣本來源的限制，不可能完成對所有相關或相似物質交叉反應檢測，因此本試劑盒有可能與未經(jīng)檢測的其它物質有交叉反應。
回收率
分別于定值血清及血漿樣本中加入一定量的白蛋白(ALB)（加標樣品），重復測定并計算其均值，回收率為測定值與理論值的比率。
| 樣本 | 回收率范圍(%) | 平均回收率(%) |
| serum(n=5) | 91-105 | 97 |
| EDTA plasma(n=5) | 90-98 | 95 |
| heparin plasma(n=5) | 97-104 | 101 |
精密度
精密度用樣品測定值的變異系數(shù)CV表示。CV(%) = SD/mean×100
批內差：取同批次試劑盒對低、中、高值定值樣本進行定量檢測,每份樣本連續(xù)測定20 次，分別計算不同濃度樣本的平均值及SD值。
批間差：選取3個不同批次的試劑盒分別對低、中、高值定值樣本進行定量測定，每個樣本使用同一試劑盒重復測定8次，分別計算不同濃度樣本的平均值及SD值。
批內差： CV<10%
批間差： CV<12%
線性
在定值血清及血漿樣本內加入適量的白蛋白(ALB)，并倍比稀釋成1:2，1:4，1:8，1:16的待測樣本，線性范圍即為稀釋后樣本中白蛋白(ALB)含量的測定值與理論值的比率。
| 樣本 | 1:2 | 1:4 | 1:8 | 1:16 |
| serum(n=5) | 90-98% | 86-101% | 79-97% | 86-104% |
| EDTA plasma(n=5) | 83-92% | 93-101% | 98-105% | 94-102% |
| heparin plasma(n=5) | 84-103% | 97-105% | 79-102% | 98-105% |
穩(wěn)定性
經(jīng)測定，試劑盒在有效期內按推薦溫度保存，其活性降低率小于5%。
為減小外部因素對試劑盒破壞前后檢測值的影響，實驗室的環(huán)境條件需盡量保持一致，尤其是實驗室內溫度、濕度及溫育條件。其次由同一實驗員來進行操作可減少人為誤差。
實驗流程
1. 實驗前標準品、試劑及樣本的準備；
2. 加樣（標準品及樣本）50µL，
加入50µL檢測液A(臨用前配制);
37°C溫育1小時。
3. 洗板3次；
4. 加檢測溶液B100µL，37°C孵育30分鐘；
5. 洗板5次；
6. 加TMB底物90µL，37°C孵育10-20分鐘；
7. 加終止液50µL，立即450nm讀數(shù)。
實驗原理
本試劑盒應用競爭抑制酶聯(lián)免疫分析法測定標本中待測物質水平。將白蛋白(ALB)單克隆抗體包被微孔板，制成固相載 體，往包被抗體的微孔中同時加入生物素標記的抗原和待測抗原(標準品或樣本)，待測抗原與生物素標記抗原對特異性抗體進行競爭結合。溫育后經(jīng)洗滌去掉未結合物，然后加入HRP標記的親和素，經(jīng)過溫育和徹底洗滌后加入底物TMB顯色。TMB在過氧化物酶的催化下轉化成藍色，并在酸的作用下轉化成最終的黃色。待測標本濃度越高，標記抗原和抗體的結合就越受到抑制，顯色愈淺。顯色的深淺與酶量呈正相關，而與樣品中待測物質含量呈負相關。用酶標儀在450nm波長下測定吸光度（O.D.值），計算樣品濃度。
增值服務
相關產(chǎn)品
| 編號 | 適用物種：Bos taurus; Bovine (Cattle，牛) | 應用(僅供研究使用，不用于臨床診斷！) |
| NPB028Bo01 | 白蛋白(ALB)天然蛋白 | Positive Control; Immunogen; SDS-PAGE; WB. |
| PAB028Bo01 | 白蛋白(ALB)多克隆抗體 | WB; IHC; ICC; IP. |
| LAB028Bo71 | 白蛋白(ALB)多克隆抗體(生物素標記) | WB; IHC; ICC. |
| CEB028Bo | 白蛋白(ALB)檢測試劑盒(酶聯(lián)免疫吸附試驗法) | Enzyme-linked immunosorbent assay for Antigen Detection. |
| KSB028Bo11 | 白蛋白(ALB)檢測試劑盒DIY材料(酶聯(lián)免疫吸附試驗法) | Main materials for "Do It (ELISA Kit) Yourself". |
參考文獻
| 雜志 | 參考文獻 |
| PLoS One | Simvastatin Reduces Endotoxin-Induced Acute Lung Injury by Decreasing Neutrophil Recruitment and Radical Formation [PubMed: 22701728] |
| Liver International | Albumin administration protects against bilirubin-induced auditory brainstem dysfunction in Gunn rat pups [Onlinelibrary: liv.12219] |
| Journal of Infectious Diseases | Adjunctive corticosteroid therapy improves lung immunopathology and survival during severe secondary pneumococcal pneumonia in mice [Pubmed: 24273183] |
| Toxins (Basel). | Increased Circulating Levels of Vitamin D Binding Protein in MS Patients [Pubmed:25590278] |
| Mol Cell Biochem | The wnt/β-catenin signaling pathway participates in rhein ameliorating kidney injury in DN mice [PubMed: 26346164] |
| PLoS?One | Collecting Protein Biomarkers in Breath Using Electret Filters: A Preliminary Method on New Technical Model and Human Study [Pubmed:26934615] |
| Journal of Biomaterials and Tissue Engineering | 3D Printing of Differentiated Bone Marrow Mesenchymal Cells as a New Method for Liver Tissue Engineering [art00007] |
| Experimental and molecular pathology | Alteration of Connexin43 expression in a rat model of obesity-related glomerulopathy. [pubmed:29246788] |
| Inflammation | Resolvin D1 Promotes SIRT1 Expression to Counteract the Activation of STAT3 and NF-κB in Mice with Septic-Associated Lung Injury [Pubmed:30014231] |
| Journal of Developmental Origins of?Health?and?Disease | Maternal low-quality protein diet exerts sex-specific effects on plasma amino acid profile and alters hepatic expression of methyltransferases in adult rat offspring [Pubmed:29582727] |
| Medicine | Electret filter collects more exhaled albumin than glass condenser: A method comparison based on human study [Pubmed:29384875] |
| Stem?Cell?Research?&?Therapy | Therapeutic efficiency of human amniotic epithelial stem cell-derived functional hepatocyte-like cells in mice with acute hepatic failure [Pubmed: 30463600] |
| JOURNAL OF BIOMEDICAL MATERIALS RESEARCH PART A | Decellularized Human Liver Scaffold‐Based Three‐dimensional Culture System Facilitate HBV Infection [Pubmed: 30963688] |
| biomedicine & pharmacotherapy | Treatment with 3, 4-dihydroxyphenylethyl alcohol glycoside ameliorates sepsis-induced ALI in mice by reducing inflammation and regulating M1 polarization [Pubmed: 31146107] |
| biochemical and biophysical research communications | Anti-proteinuria effect of antibody against ANGPTL3 coil-coiled domain on adriamycin-induced nephropathy in mice [Pubmed: 31256934] |
| Diabetes, Metabolic Syndrome and Obesity | A small molecule inhibitor MCC950 ameliorates kidney injury in diabetic nephropathy by inhibiting NLRP3 inflammasome activation [Pubmed: 31447572] |
| Molecular and Cellular Endocrinology | Diiodothyronines regulate metabolic homeostasis in primary human hepatocytes by modulating mTORC1 and mTORC2 activity [Pubmed: 31580898] |
| BMC Veterinary Research | Preliminary study on the effects of enrofloxacin, flunixin meglumine and pegbovigrastim on Mycoplasma bovis pneumonia [Pubmed: 31655595] |
| International Immunopharmacology | Ghrelin alleviates traumatic brain injury-induced acute lung injury through pyroptosis/NF-κB pathway [Pubmed: 31918060] |
| HYPERTENSION RESEARCH | Sodium butyrate ameliorates deoxycorticosterone acetate/salt-induced hypertension and renal damage by inhibiting the MR/SGK1 pathway [Pubmed: 32908237] |
| Obesity Surgery | Sleeve Gastrectomy Attenuates Diabetic Nephropathy by Upregulating Nephrin Expressions in Diabetic Obese Rats [Pubmed: 32399849] |
| Protective Role of Training and Selenium Consumption Against Renal Toxicity Induced by Cadmium in Rats [] | |
| Nutrition | Gut Dysbiosis Contribute to High Fructose Induced Salt Sensitive Hypertension In Sprague-Dawley Rats [] |
| sensors and actuators b-chemical | A light-induced self-powered competitive immunosensor for the detection of platelet derived growth factor-BB via an elaborately assembled bioconjugate [] |
| Biochem Biophys Res Commun | cGAS promotes sepsis in radiotherapy of cancer by up-regulating caspase-11 signaling [33721834] |
| Journal of Chinese Pharmaceutical Sciences | A simple and sensitive electrochemical impedimetric immunosensor for the detection of apolipoprotein E4 based on CeZnO nanoparticle [] |
| Inflammation | Syringaresinol Resisted Sepsis-Induced Acute Lung Injury by Suppressing Pyroptosis Via the Oestrogen Receptor-β Signalling Pathway [34807349] |
| Nat Commun | GP73 is a TBC-domain Rab GTPase-activating protein contributing to the pathogenesis of non-alcoholic fatty liver disease without obesity [34853313] |
| ACS Appl Mater Interfaces | Emitter–Quencher Pair of Single Atomic Co Sites and Monolayer Titanium Carbide MXenes for Luminol Chemiluminescent Reactions [34914377] |
| Food & Function | Moderation of gut microbiota and bile acid metabolism by chlorogenic acid improves high-fructose-induced salt-sensitive hypertension in mice [Pubmed:35708145] |
| Authorea | Syringaresinol attenuates kidney injury by inhibition of pyroptosis via activating the Nrf2 antioxidant pathway in diabetic nephropathy [] |
