肌酸激酶MB同工酶(CKMB)檢測試劑盒(酶聯(lián)免疫吸附試驗(yàn)法)
ELISA Kit for Creatine Kinase MB Isoenzyme (CKMB)
CK2; CK-MB; Creatine Kinase,Muscle/Brain
- 編號SEA479Hu
- 物種Homo sapiens (Human，人) 相同的名稱，不同的物種。
- 實(shí)驗(yàn)方法雙抗夾心
- 反應(yīng)時(shí)長3h
- 檢測范圍1.56-100ng/mL
- 靈敏度最小可檢測劑量小于等于0.59ng/mL.
- 樣本類型serum, plasma, tissue homogenates, cell lysates, cell culture supernates and other biological fluids
- 下載 英文說明書 中文說明書
- 規(guī)格 48T96T 96T*5 96T*10 96T*100
- 價(jià)格 ¥ 2646 ¥ 3780 ¥ 17010 ¥ 32130 ¥ 264600
- 欲了解實(shí)際交易價(jià)格和更多情況，請與當(dāng)?shù)亟?jīng)銷商聯(lián)系!
-
產(chǎn)品包裝(模擬)
-
產(chǎn)品包裝(模擬)
-
實(shí)驗(yàn)結(jié)果圖
-
標(biāo)準(zhǔn)曲線圖
-
通過ISO 9001、ISO 13485質(zhì)量體系認(rèn)證
特異性
本試劑盒用于檢測肌酸激酶MB同工酶(CKMB)，經(jīng)檢測與其它相似物質(zhì)無明顯交叉反應(yīng)。
由于受到技術(shù)及樣本來源的限制，不可能完成對所有相關(guān)或相似物質(zhì)交叉反應(yīng)檢測，因此本試劑盒有可能與未經(jīng)檢測的其它物質(zhì)有交叉反應(yīng)。
回收率
分別于定值血清及血漿樣本中加入一定量的肌酸激酶MB同工酶(CKMB)（加標(biāo)樣品），重復(fù)測定并計(jì)算其均值，回收率為測定值與理論值的比率。
| 樣本 | 回收率范圍(%) | 平均回收率(%) |
| serum(n=5) | 95-104 | 101 |
| EDTA plasma(n=5) | 91-99 | 96 |
| heparin plasma(n=5) | 86-101 | 94 |
精密度
精密度用樣品測定值的變異系數(shù)CV表示。CV(%) = SD/mean×100
批內(nèi)差：取同批次試劑盒對低、中、高值定值樣本進(jìn)行定量檢測,每份樣本連續(xù)測定20 次，分別計(jì)算不同濃度樣本的平均值及SD值。
批間差：選取3個(gè)不同批次的試劑盒分別對低、中、高值定值樣本進(jìn)行定量測定，每個(gè)樣本使用同一試劑盒重復(fù)測定8次，分別計(jì)算不同濃度樣本的平均值及SD值。
批內(nèi)差： CV<10%
批間差： CV<12%
線性
在定值血清及血漿樣本內(nèi)加入適量的肌酸激酶MB同工酶(CKMB)，并倍比稀釋成1:2，1:4，1:8，1:16的待測樣本，線性范圍即為稀釋后樣本中肌酸激酶MB同工酶(CKMB)含量的測定值與理論值的比率。
| 樣本 | 1:2 | 1:4 | 1:8 | 1:16 |
| serum(n=5) | 87-101% | 82-105% | 97-105% | 78-91% |
| EDTA plasma(n=5) | 84-102% | 80-96% | 96-105% | 89-97% |
| heparin plasma(n=5) | 96-103% | 92-101% | 94-105% | 80-95% |
穩(wěn)定性
經(jīng)測定，試劑盒在有效期內(nèi)按推薦溫度保存，其活性降低率小于5%。
為減小外部因素對試劑盒破壞前后檢測值的影響，實(shí)驗(yàn)室的環(huán)境條件需盡量保持一致，尤其是實(shí)驗(yàn)室內(nèi)溫度、濕度及溫育條件。其次由同一實(shí)驗(yàn)員來進(jìn)行操作可減少人為誤差。
實(shí)驗(yàn)流程
1. 實(shí)驗(yàn)前標(biāo)準(zhǔn)品、試劑及樣本的準(zhǔn)備；
2. 加樣（標(biāo)準(zhǔn)品及樣本）100µL，37°C孵育1小時(shí)；
3. 吸棄，加檢測溶液A100µL，37°C孵育1小時(shí)；
4. 洗板3次；
5. 加檢測溶液B100µL，37°C孵育30分鐘；
6. 洗板5次；
7. 加TMB底物90µL，37°C孵育10-20分鐘；
8. 加終止液50µL，立即450nm讀數(shù)。
實(shí)驗(yàn)原理
將CKMM抗體包被于96孔微孔板中，制成固相載體，向微孔中分別加入標(biāo)準(zhǔn)品或標(biāo)本，其中CKMB與連接于固相載體上的抗體結(jié)合，然后加入生物素化的CKBB抗體，在將未結(jié)合的生物素化抗體洗凈后，加入HRP標(biāo)記的親和素，再次徹底洗滌后加入TMB底物顯色。TMB在過氧化物酶的催化下轉(zhuǎn)化成藍(lán)色，并在酸的作用下轉(zhuǎn)化成最終的黃色。顏色的深淺和樣品中的CKMB濃度呈正相關(guān)。用酶標(biāo)儀在450nm波長下測定吸光度（O.D.值），計(jì)算樣品濃度。
增值服務(wù)
相關(guān)產(chǎn)品
| 編號 | 適用物種：Homo sapiens (Human，人) | 應(yīng)用(僅供研究使用，不用于臨床診斷！) |
| SEA479Hu | 肌酸激酶MB同工酶(CKMB)檢測試劑盒(酶聯(lián)免疫吸附試驗(yàn)法) | Enzyme-linked immunosorbent assay for Antigen Detection. |
| LMA479Hu | 肌酸激酶MB同工酶(CKMB)等多因子檢測試劑盒(流式熒光發(fā)光法) | FLIA Kit for Antigen Detection. |
參考文獻(xiàn)
| 雜志 | 參考文獻(xiàn) |
| Cardiovascular Toxicology | Aldehyde Dehydrogenase-2 Activation during Cardioplegic Arrest Enhances the Cardioprotection against Myocardial Ischemia–Reperfusion Injury [PubMed: 22814936] |
| PLoS One | N-Acetylcysteine and Allopurinol Confer Synergy in Attenuating Myocardial Ischemia Injury via Restoring HIF-1α/HO-1 Signaling in Diabetic Rats [PubMed: PMC3715528] |
| Int J Clin Exp Med | Effects of morphine and sufentanil preconditioning against myocardial ischemic-reperfusion injury in rabbits [PubMed: 26629064] |
| European Journal of Pharmacology | Diabetes blocks the cardioprotective effects of sevoflurane postconditioning by impairing Nrf2/Brg1/HO-1 signaling [Pubmed:26973173] |
| Asian Pac J Trop Med.? | Effect of levocarnitine on TIMP-1, ICAM-1 expression of rats with coronary heart disease and its myocardial protection effect [Pubmed:26972400] |
| Heart International | Circulating miR-126 and miR-499 reflect progression of cardiovascular disease; correlations with uric acid and ejection fraction [pubmed:27924211] |
| American Journal of Translational?Research | Peroxisome proliferator-activated receptor γ (PPARγ) mediates the protective effect of quercetin against myocardial ischemia-reperfusion injury via … [PMC5209473] |
| Archivum Immunologiae et Therapiae Experimentalis | In?Vivo?Cardioprotective?Effects?and?Pharmacokinetic?Profile?of?N-Propyl?Caffeamide?AgainstIschemia?Reperfusion?Injury. [pubmed:27480649] |
| Archivos de medicina veterinaria | Cardiac biomarkers in dogs with visceral leishmaniasis [S0301-732X2016000300004] |
| Cellular Physiology and Biochemistry | Remote Limb Ischaemic Postconditioning Protects Against Myocardial Ischaemia/ Reperfusion Injury in Mice: Activation of JAK/STAT3-Mediated Nrf2-Antioxidant Signalling [10.1159:481755] |
| Erciyes Medical Journal | Morinda Citrifolia (Noni) and Low Dose Aspirin Prevent Apoptotic Cell Death and Oxidative Stress on Isoproterenol Induced Myocardial Infarction in Rats [DOI: 10.5152/etd.2017.1732] |
| International Immunopharmacology | Metformin attenuated endotoxin-induced acute myocarditis via activating AMPK [pubmed:28410530] |
| Science?of the?Total?Environment | Co-exposure of silica nanoparticles and methylmercury induced cardiac toxicity and [Pubmed:29727991] |
| Journal of Surgical Research | GYY4137 protects against myocardial ischemia/reperfusion injury via activation of the PHLPP-1/Akt/Nrf2 signaling pathway in diabetic mice [Pubmed:29605032] |
| Molecular?Medicine?Reports? | Hydrogen?rich solution against myocardial injury and aquaporin expression via the PI3K/Akt signaling pathway during cardiopulmonary bypass in rats [Pubmed:29956781] |
| Cell?Death?Discovery | Cyclic helix B peptide ameliorates acute myocardial infarction in mice by inhibiting apoptosis and inflammatory responses [] |
| Oxidative Medicine and Cellular Longevity | Adiponectin Facilitates Postconditioning Cardioprotection through Both AMPK-Dependent Nuclear and AMPK-Independent Mitochondrial STAT3 Activation [Pubmed: 32190173] |
| PLoS One | Tissue suction-mediated gene transfer to the beating heart in mice [Pubmed: 32027678] |
| FASEB JOURNAL | Deletion of Rap1 protects against myocardial ischemia/reperfusion injury through suppressing cell apoptosis via activation of STAT3 signaling [Pubmed: 32020680] |
| Molecular Medicine Reports | Differentially expressed lncRNAs, miRNAs and mRNAs with associated ceRNA networks in a mouse model of myocardial ischemia/reperfusion injury [Pubmed: 32705277] |
| In Vivo | Hydrogen sulphide and nitric oxide cooperate in cardioprotection against ischemia/reperfusion injury in isolated rat heart [Pubmed: 32871779] |
| Drug Design Development and Therapy | Melatonin Attenuates Anoxia/Reoxygenation Injury by Inhibiting Excessive Mitophagy Through the MT2/SIRT3/FoxO3a Signaling Pathway in H9c2 Cells [Pubmed: 32546969] |
| Nanomaterials | Biological Safety and Biodistribution of Chitosan Nanoparticles [Pubmed: 32340313] |
| Molecular Medicine Reports | The mRNA expression of Il6 and Pdcd1 are predictive and protective factors for doxorubicin?induced cardiotoxicity [33300057] |
| Oxid Med Cell Longev | Inactivation of TOPK Caused by Hyperglycemia Blocks Diabetic Heart Sensitivity to Sevoflurane Postconditioning by Impairing the PTEN/PI3K/Akt Signaling [33986917] |
| Eur J Pharmacol | Long non-coding RNA MALAT1 modulates myocardial ischemia-reperfusion injury though the PI3K/Akt/eNOS pathway by sponging miRNA-133a-3p to target IGF1R?… [Pubmed:34968461] |
| Cardiovasc Drugs Ther | Hyperglycemia-Induced Overexpression of PH Domain Leucine-Rich Repeat Protein Phosphatase 1 (PHLPP1) Compromises the Cardioprotective Effect of Ischemic?… [Pubmed:35715527] |
| Nutrients | Mokko Lactone Alleviates Doxorubicin-Induced Cardiotoxicity in Rats via Antioxidant, Anti-Inflammatory, and Antiapoptotic Activities. Nutrients 2022, 14, 733 [Pubmed:35215383] |
| Experimental and Therapeutic Medicine | Hydrogen therapy after resuscitation improves myocardial injury involving inhibition of autophagy in an asphyxial rat model of cardiac arrest [Pubmed:35495584] |
| Oxidative Medicine and Cellular Longevity | MicroRNA-503 Exacerbates Myocardial Ischemia/Reperfusion Injury via Inhibiting PI3K/Akt-and STAT3-Dependent Prosurvival Signaling Pathways [Pubmed:35620576] |
| International Journal of Sciences: Basic and Applied Research | Vitamin E Attenuates Cardiomyopathy Via Alleviation of Autophagic Stress [] |
