抗白蛋白抗體(Anti-ALB)檢測試劑盒(酶聯(lián)免疫吸附試驗法)
ELISA Kit for Anti-Albumin Antibody (Anti-ALB)
Serum albumin
- 編號AEB028Hu
- 物種Homo sapiens (Human，人) 相同的名稱，不同的物種。
- 實驗方法間接法
- 反應(yīng)時長2h, 30min
- 檢測范圍31.2-2,000pg/mL
- 靈敏度最小可檢測劑量小于等于12.3pg/mL.
- 樣本類型serum, plasma and other biological fluids
- 下載 英文說明書 中文說明書
- 規(guī)格 48T96T 96T*5 96T*10 96T*100
- 價格 ¥ 3360 ¥ 4800 ¥ 21600 ¥ 40800 ¥ 336000
- 欲了解實際交易價格和更多情況，請與當(dāng)?shù)亟?jīng)銷商聯(lián)系!
-
產(chǎn)品包裝(模擬)
-
產(chǎn)品包裝(模擬)
-
實驗結(jié)果圖
-
標(biāo)準(zhǔn)曲線圖
-
通過ISO 9001、ISO 13485質(zhì)量體系認(rèn)證
特異性
本試劑盒用于檢測抗白蛋白抗體(Anti-ALB)，經(jīng)檢測與其它相似物質(zhì)無明顯交叉反應(yīng)。
由于受到技術(shù)及樣本來源的限制，不可能完成對所有相關(guān)或相似物質(zhì)交叉反應(yīng)檢測，因此本試劑盒有可能與未經(jīng)檢測的其它物質(zhì)有交叉反應(yīng)。
回收率
分別于定值血清及血漿樣本中加入一定量的抗白蛋白抗體(Anti-ALB)（加標(biāo)樣品），重復(fù)測定并計算其均值，回收率為測定值與理論值的比率。
| 樣本 | 回收率范圍(%) | 平均回收率(%) |
| serum(n=5) | 79-93 | 87 |
| EDTA plasma(n=5) | 92-101 | 97 |
| heparin plasma(n=5) | 80-103 | 101 |
精密度
精密度用樣品測定值的變異系數(shù)CV表示。CV(%) = SD/mean×100
批內(nèi)差：取同批次試劑盒對低、中、高值定值樣本進(jìn)行定量檢測,每份樣本連續(xù)測定20 次，分別計算不同濃度樣本的平均值及SD值。
批間差：選取3個不同批次的試劑盒分別對低、中、高值定值樣本進(jìn)行定量測定，每個樣本使用同一試劑盒重復(fù)測定8次，分別計算不同濃度樣本的平均值及SD值。
批內(nèi)差： CV<10%
批間差： CV<12%
線性
在定值血清及血漿樣本內(nèi)加入適量的抗白蛋白抗體(Anti-ALB)，并倍比稀釋成1:2，1:4，1:8，1:16的待測樣本，線性范圍即為稀釋后樣本中抗白蛋白抗體(Anti-ALB)含量的測定值與理論值的比率。
| 樣本 | 1:2 | 1:4 | 1:8 | 1:16 |
| serum(n=5) | 89-103% | 94-102% | 83-103% | 80-91% |
| EDTA plasma(n=5) | 85-97% | 95-105% | 89-102% | 93-105% |
| heparin plasma(n=5) | 87-101% | 91-101% | 81-103% | 90-102% |
穩(wěn)定性
經(jīng)測定，試劑盒在有效期內(nèi)按推薦溫度保存，其活性降低率小于5%。
為減小外部因素對試劑盒破壞前后檢測值的影響，實驗室的環(huán)境條件需盡量保持一致，尤其是實驗室內(nèi)溫度、濕度及溫育條件。其次由同一實驗員來進(jìn)行操作可減少人為誤差。
實驗流程
1. 實驗前標(biāo)準(zhǔn)品、試劑及樣本的準(zhǔn)備；
2. 加樣（標(biāo)準(zhǔn)品及樣本）100µL，37°C孵育1小時；
3. 吸棄，加檢測溶液A100μL，37°C孵育1小時；
4. 洗板5次；
5. 加TMB底物90µL，37°C孵育10-20分鐘；
6. 加終止液50µL，立即450nm讀數(shù)。
實驗原理
將抗原包被于96孔微孔板中，制成固相載體，向微孔中分別加入標(biāo)準(zhǔn)品或標(biāo)本，其中的抗白蛋白抗體(Anti-ALB)與連接于固相載體上的抗原結(jié)合，然后加入HRP標(biāo)記的二抗，徹底洗滌后加入TMB底物顯色。TMB在過氧化物酶的催化下轉(zhuǎn)化成藍(lán)色，并在酸的作用下轉(zhuǎn)化成最終的黃色。顏色的深淺和樣品中的抗白蛋白抗體(Anti-ALB)呈正相關(guān)。用酶標(biāo)儀在450nm波長下測定吸光度(O.D.值)，計算樣品濃度。
增值服務(wù)
相關(guān)產(chǎn)品
| 編號 | 適用物種：Homo sapiens (Human，人) | 應(yīng)用(僅供研究使用，不用于臨床診斷！) |
| NPB028Hu01 | 白蛋白(ALB)天然蛋白 | Positive Control; Immunogen; SDS-PAGE; WB. |
| NPB028Hu02 | 白蛋白(ALB)天然蛋白 | Positive Control; Immunogen; SDS-PAGE; WB. |
| RPB028Hu01 | 白蛋白(ALB)重組蛋白 | Positive Control; Immunogen; SDS-PAGE; WB. |
| EPB028Hu51 | 白蛋白(ALB)真核蛋白 | Positive Control; Immunogen; SDS-PAGE; WB. |
| RPB028Hu51 | 白蛋白(ALB)重組蛋白 | Positive Control; Immunogen; SDS-PAGE; WB. |
| PAB028Hu07 | 白蛋白(ALB)多克隆抗體 | WB; IHC; ICC; IP. |
| PAB028Hu05 | 白蛋白(ALB)多克隆抗體 | WB; IHC; ICC; IP. |
| PAB028Hu51 | 白蛋白(ALB)多克隆抗體 | WB; IHC; ICC; IP. |
| PAB028Hu01 | 白蛋白(ALB)多克隆抗體 | WB; IHC; ICC; IP. |
| PAB028Hu02 | 白蛋白(ALB)多克隆抗體 | WB; IHC; ICC; IP. |
| LAB028Hu71 | 白蛋白(ALB)多克隆抗體(生物素標(biāo)記) | WB; IHC; ICC. |
| LAB028Hu81 | 白蛋白(ALB)多克隆抗體(異硫氰酸熒光素標(biāo)記) | WB; IHC; ICC; IF. |
| MAB028Hu23 | 白蛋白(ALB)單克隆抗體 | WB; IHC; ICC; IP. |
| MAB028Hu22 | 白蛋白(ALB)單克隆抗體 | WB; IHC |
| MAB028Hu21 | 白蛋白(ALB)單克隆抗體 | WB; IHC; ICC; IP. |
| HEB028Hu | 白蛋白(ALB)檢測試劑盒(酶聯(lián)免疫吸附試驗法,高敏型) | Enzyme-linked immunosorbent assay for Antigen Detection. |
| CEB028Hu | 白蛋白(ALB)檢測試劑盒(酶聯(lián)免疫吸附試驗法) | Enzyme-linked immunosorbent assay for Antigen Detection. |
| AEB028Hu | 抗白蛋白抗體(Anti-ALB)檢測試劑盒(酶聯(lián)免疫吸附試驗法) | Enzyme-linked immunosorbent assay for Antibody Detection. |
| ACB028Hu | 抗白蛋白抗體(Anti-ALB)檢測試劑盒(化學(xué)發(fā)光免疫分析法) | Chemiluminescent immunoassay for Antibody Detection. |
| CCB028Hu | 白蛋白(ALB)檢測試劑盒(化學(xué)發(fā)光免疫分析法) | Chemiluminescent immunoassay for Antigen Detection. |
| LMB028Hu | 白蛋白(ALB)等多因子檢測試劑盒(流式熒光發(fā)光法) | FLIA Kit for Antigen Detection. |
| KSB028Hu11 | 白蛋白(ALB)檢測試劑盒DIY材料(酶聯(lián)免疫吸附試驗法) | Main materials for "Do It (ELISA Kit) Yourself". |
參考文獻(xiàn)
| 雜志 | 參考文獻(xiàn) |
| PLoS One | Simvastatin Reduces Endotoxin-Induced Acute Lung Injury by Decreasing Neutrophil Recruitment and Radical Formation [PubMed: 22701728] |
| Liver International | Albumin administration protects against bilirubin-induced auditory brainstem dysfunction in Gunn rat pups [Onlinelibrary: liv.12219] |
| Journal of Infectious Diseases | Adjunctive corticosteroid therapy improves lung immunopathology and survival during severe secondary pneumococcal pneumonia in mice [Pubmed: 24273183] |
| Toxins (Basel). | Increased Circulating Levels of Vitamin D Binding Protein in MS Patients [Pubmed:25590278] |
| Mol Cell Biochem | The wnt/β-catenin signaling pathway participates in rhein ameliorating kidney injury in DN mice [PubMed: 26346164] |
| PLoS?One | Collecting Protein Biomarkers in Breath Using Electret Filters: A Preliminary Method on New Technical Model and Human Study [Pubmed:26934615] |
| Journal of Biomaterials and Tissue Engineering | 3D Printing of Differentiated Bone Marrow Mesenchymal Cells as a New Method for Liver Tissue Engineering [art00007] |
| Experimental and molecular pathology | Alteration of Connexin43 expression in a rat model of obesity-related glomerulopathy. [pubmed:29246788] |
| Inflammation | Resolvin D1 Promotes SIRT1 Expression to Counteract the Activation of STAT3 and NF-κB in Mice with Septic-Associated Lung Injury [Pubmed:30014231] |
| Journal of Developmental Origins of?Health?and?Disease | Maternal low-quality protein diet exerts sex-specific effects on plasma amino acid profile and alters hepatic expression of methyltransferases in adult rat offspring [Pubmed:29582727] |
| Medicine | Electret filter collects more exhaled albumin than glass condenser: A method comparison based on human study [Pubmed:29384875] |
| Stem?Cell?Research?&?Therapy | Therapeutic efficiency of human amniotic epithelial stem cell-derived functional hepatocyte-like cells in mice with acute hepatic failure [Pubmed: 30463600] |
| JOURNAL OF BIOMEDICAL MATERIALS RESEARCH PART A | Decellularized Human Liver Scaffold‐Based Three‐dimensional Culture System Facilitate HBV Infection [Pubmed: 30963688] |
| biomedicine & pharmacotherapy | Treatment with 3, 4-dihydroxyphenylethyl alcohol glycoside ameliorates sepsis-induced ALI in mice by reducing inflammation and regulating M1 polarization [Pubmed: 31146107] |
| biochemical and biophysical research communications | Anti-proteinuria effect of antibody against ANGPTL3 coil-coiled domain on adriamycin-induced nephropathy in mice [Pubmed: 31256934] |
| Diabetes, Metabolic Syndrome and Obesity | A small molecule inhibitor MCC950 ameliorates kidney injury in diabetic nephropathy by inhibiting NLRP3 inflammasome activation [Pubmed: 31447572] |
| Molecular and Cellular Endocrinology | Diiodothyronines regulate metabolic homeostasis in primary human hepatocytes by modulating mTORC1 and mTORC2 activity [Pubmed: 31580898] |
| BMC Veterinary Research | Preliminary study on the effects of enrofloxacin, flunixin meglumine and pegbovigrastim on Mycoplasma bovis pneumonia [Pubmed: 31655595] |
| International Immunopharmacology | Ghrelin alleviates traumatic brain injury-induced acute lung injury through pyroptosis/NF-κB pathway [Pubmed: 31918060] |
| HYPERTENSION RESEARCH | Sodium butyrate ameliorates deoxycorticosterone acetate/salt-induced hypertension and renal damage by inhibiting the MR/SGK1 pathway [Pubmed: 32908237] |
| Obesity Surgery | Sleeve Gastrectomy Attenuates Diabetic Nephropathy by Upregulating Nephrin Expressions in Diabetic Obese Rats [Pubmed: 32399849] |
| Protective Role of Training and Selenium Consumption Against Renal Toxicity Induced by Cadmium in Rats [] | |
| Nutrition | Gut Dysbiosis Contribute to High Fructose Induced Salt Sensitive Hypertension In Sprague-Dawley Rats [] |
| sensors and actuators b-chemical | A light-induced self-powered competitive immunosensor for the detection of platelet derived growth factor-BB via an elaborately assembled bioconjugate [] |
| Biochem Biophys Res Commun | cGAS promotes sepsis in radiotherapy of cancer by up-regulating caspase-11 signaling [33721834] |
| Journal of Chinese Pharmaceutical Sciences | A simple and sensitive electrochemical impedimetric immunosensor for the detection of apolipoprotein E4 based on CeZnO nanoparticle [] |
| Inflammation | Syringaresinol Resisted Sepsis-Induced Acute Lung Injury by Suppressing Pyroptosis Via the Oestrogen Receptor-β Signalling Pathway [34807349] |
| Nat Commun | GP73 is a TBC-domain Rab GTPase-activating protein contributing to the pathogenesis of non-alcoholic fatty liver disease without obesity [34853313] |
| ACS Appl Mater Interfaces | Emitter–Quencher Pair of Single Atomic Co Sites and Monolayer Titanium Carbide MXenes for Luminol Chemiluminescent Reactions [34914377] |
| Food & Function | Moderation of gut microbiota and bile acid metabolism by chlorogenic acid improves high-fructose-induced salt-sensitive hypertension in mice [Pubmed:35708145] |
| Authorea | Syringaresinol attenuates kidney injury by inhibition of pyroptosis via activating the Nrf2 antioxidant pathway in diabetic nephropathy [] |
留言咨詢
