堿性成纖維細(xì)胞生長因子(FGF2)檢測試劑盒(酶聯(lián)免疫吸附試驗(yàn)法)
ELISA Kit for Fibroblast Growth Factor 2, Basic (FGF2)
B-FGF; BFGF; FGFB; HBGH-2; Basic Fibroblast Growth Factor; Heparin-binding growth factor 2
- 編號(hào)CEA551Hu
- 物種Homo sapiens (Human，人) 相同的名稱，不同的物種。
- 實(shí)驗(yàn)方法競爭抑制
- 反應(yīng)時(shí)長2h
- 檢測范圍12.35-1,000pg/mL
- 靈敏度最小可檢測劑量小于等于4.43pg/mL.
- 樣本類型serum, plasma, tissue homogenates, cell lysates, cell culture supernates and other biological fluids
- 下載 英文說明書 中文說明書
- 規(guī)格 48T96T 96T*5 96T*10 96T*100
- 價(jià)格 ¥ 1966 ¥ 2808 ¥ 12636 ¥ 23868 ¥ 196560
- 欲了解實(shí)際交易價(jià)格和更多情況，請(qǐng)與當(dāng)?shù)亟?jīng)銷商聯(lián)系!
-
產(chǎn)品包裝(模擬)
-
產(chǎn)品包裝(模擬)
-
實(shí)驗(yàn)結(jié)果圖
-
標(biāo)準(zhǔn)曲線圖
-
通過ISO 9001、ISO 13485質(zhì)量體系認(rèn)證
特異性
本試劑盒用于檢測堿性成纖維細(xì)胞生長因子(FGF2)，經(jīng)檢測與其它相似物質(zhì)無明顯交叉反應(yīng)。
由于受到技術(shù)及樣本來源的限制，不可能完成對(duì)所有相關(guān)或相似物質(zhì)交叉反應(yīng)檢測，因此本試劑盒有可能與未經(jīng)檢測的其它物質(zhì)有交叉反應(yīng)。
精密度
精密度用樣品測定值的變異系數(shù)CV表示。CV(%) = SD/mean×100
批內(nèi)差：取同批次試劑盒對(duì)低、中、高值定值樣本進(jìn)行定量檢測,每份樣本連續(xù)測定20 次，分別計(jì)算不同濃度樣本的平均值及SD值。
批間差：選取3個(gè)不同批次的試劑盒分別對(duì)低、中、高值定值樣本進(jìn)行定量測定，每個(gè)樣本使用同一試劑盒重復(fù)測定8次，分別計(jì)算不同濃度樣本的平均值及SD值。
批內(nèi)差： CV<10%
批間差： CV<12%
穩(wěn)定性
經(jīng)測定，試劑盒在有效期內(nèi)按推薦溫度保存，其活性降低率小于5%。
為減小外部因素對(duì)試劑盒破壞前后檢測值的影響，實(shí)驗(yàn)室的環(huán)境條件需盡量保持一致，尤其是實(shí)驗(yàn)室內(nèi)溫度、濕度及溫育條件。其次由同一實(shí)驗(yàn)員來進(jìn)行操作可減少人為誤差。
實(shí)驗(yàn)流程
1. 實(shí)驗(yàn)前標(biāo)準(zhǔn)品、試劑及樣本的準(zhǔn)備；
2. 加樣（標(biāo)準(zhǔn)品及樣本）50µL，
加入50µL檢測液A(臨用前配制);
37°C溫育1小時(shí)。
3. 洗板3次；
4. 加檢測溶液B100µL，37°C孵育30分鐘；
5. 洗板5次；
6. 加TMB底物90µL，37°C孵育10-20分鐘；
7. 加終止液50µL，立即450nm讀數(shù)。
實(shí)驗(yàn)原理
本試劑盒應(yīng)用競爭抑制酶聯(lián)免疫分析法測定標(biāo)本中待測物質(zhì)水平。將堿性成纖維細(xì)胞生長因子(FGF2)單克隆抗體包被微孔板，制成固相載體，往包被抗體的微孔中同時(shí)加入生物素標(biāo)記的抗原和待測抗原(標(biāo)準(zhǔn)品或樣本)，待測抗原與生物素標(biāo)記抗原對(duì)特異性抗體進(jìn)行競爭結(jié)合。溫育后經(jīng)洗滌去掉未結(jié)合物，然后加入HRP標(biāo)記的親和素，經(jīng)過溫育和徹底洗滌后加入底物TMB顯色。TMB在過氧化物酶的催化下轉(zhuǎn)化成藍(lán)色，并在酸的作用下轉(zhuǎn)化成最終的黃色。待測標(biāo)本濃度越高，標(biāo)記抗原和抗體的結(jié)合就越受到抑制，顯色愈淺。顯色的深淺與酶量呈正相關(guān)，而與樣品中待測物質(zhì)含量呈負(fù)相關(guān)。用酶標(biāo)儀在450nm波長下測定吸光度（O.D.值），計(jì)算樣品濃度。
贈(zèng)品
增值服務(wù)
相關(guān)產(chǎn)品
| 編號(hào) | 適用物種：Homo sapiens (Human，人) | 應(yīng)用(僅供研究使用，不用于臨床診斷！) |
| RPA551Hu02 | 堿性成纖維細(xì)胞生長因子(FGF2)重組蛋白 | Positive Control; Immunogen; SDS-PAGE; WB. |
| RPA551Hu04 | 堿性成纖維細(xì)胞生長因子(FGF2)重組蛋白 | Positive Control; Immunogen; SDS-PAGE; WB. |
| RPA551Hu01 | 堿性成纖維細(xì)胞生長因子(FGF2)重組蛋白 | Positive Control; Immunogen; SDS-PAGE; WB. |
| APA551Hu01 | 堿性成纖維細(xì)胞生長因子(FGF2)活性蛋白 | Cell?culture;?Activity?Assays. |
| CPA551Hu21 | 堿性成纖維細(xì)胞生長因子(FGF2)卵白蛋白偶聯(lián)物 | Immunogen; SDS-PAGE; WB. |
| PAA551Hu01 | 堿性成纖維細(xì)胞生長因子(FGF2)多克隆抗體 | WB; IHC; ICC; IP. |
| LAA551Hu71 | 堿性成纖維細(xì)胞生長因子(FGF2)多克隆抗體(生物素標(biāo)記) | WB; IHC; ICC. |
| LAA551Hu81 | 堿性成纖維細(xì)胞生長因子(FGF2)多克隆抗體(異硫氰酸熒光素標(biāo)記) | WB; IHC; ICC; IF. |
| MAA551Hu22 | 堿性成纖維細(xì)胞生長因子(FGF2)單克隆抗體 | WB; IHC; ICC; IP. |
| MAA551Hu21 | 堿性成纖維細(xì)胞生長因子(FGF2)單克隆抗體 | WB; IHC; ICC; IP. |
| SEA551Hu | 堿性成纖維細(xì)胞生長因子(FGF2)檢測試劑盒(酶聯(lián)免疫吸附試驗(yàn)法) | Enzyme-linked immunosorbent assay for Antigen Detection. |
| MEA551Hu | 堿性成纖維細(xì)胞生長因子(FGF2)檢測試劑盒(酶聯(lián)免疫吸附試驗(yàn)法,小樣本) | Enzyme-linked immunosorbent assay for Antigen Detection. |
| CEA551Hu | 堿性成纖維細(xì)胞生長因子(FGF2)檢測試劑盒(酶聯(lián)免疫吸附試驗(yàn)法) | Enzyme-linked immunosorbent assay for Antigen Detection. |
| HEA551Hu | 堿性成纖維細(xì)胞生長因子(FGF2)檢測試劑盒(酶聯(lián)免疫吸附試驗(yàn)法,高敏型) | Enzyme-linked immunosorbent assay for Antigen Detection. |
| LMA551Hu | 堿性成纖維細(xì)胞生長因子(FGF2)等多因子檢測試劑盒(流式熒光發(fā)光法) | FLIA Kit for Antigen Detection. |
| KSA551Hu11 | 堿性成纖維細(xì)胞生長因子(FGF2)檢測試劑盒DIY材料(酶聯(lián)免疫吸附試驗(yàn)法) | Main materials for "Do It (ELISA Kit) Yourself". |
參考文獻(xiàn)
| 雜志 | 參考文獻(xiàn) |
| Journal of Pediatric Surgery | The influence of nutrients, biliary-pancreatic secretions, and systemic trophic hormones on intestinal adaptation in a Roux-en-Y bypass model [PubMed: 20438940] |
| International Journal of Nanomedicine | Effect of heparan sulfate and gold nanoparticles on muscle development during embryogenesis [PubMed: PMC3254262] |
| Archives of Animal Nutrition | Effect of taurine and gold nanoparticles on the morphological and molecular characteristics of muscle development during chicken embryogenesis [Tandfonline: 644918] |
| Diabetes | Peroxisome Proliferator–Activated Receptor-γ Coactivator-1α (PGC-1α) Enhances Engraftment and Angiogenesis of Mesenchymal Stem Cells in Diabetic Hindlimb Ischemia [Pubmed: 22266669] |
| Molecular Pharmacology | Electrospun Fibers with Plasmid bFGF Polyplex Loadings Promote Skin Wound Healing in Diabetic Rats [Pubmed: 22091745] |
| Science Alert | Antiangiogenic Activities of Cinnamon, Black and Green Tea Extracts on Experimentally Induced Breast Cancer in Rats [Scialert: Source] |
| Nanoscale Research Letters | Silver nanoparticles administered to chicken affect VEGFA and FGF2 gene expression in breast muscle and heart. [PubMed: 22827927] |
| PLoS One | Carbon nanoparticles downregulate expression of basic fibroblast growth factor in the heart during embryogenesis [PubMed: PMC3771850] |
| International Journal of Molecular Sciences | Nano-Nutrition of Chicken Embryos. The Effect of in Ovo Administration of Diamond Nanoparticles and L-Glutamine on Molecular Responses in Chicken Embryo Pectoral Muscles [NCBI:PMC3856104] |
| Archives of Animal Nutrition | Nano-nutrition of chicken embryos. The effect of silver nanoparticles and ATP on expression of chosen genes involved in myogenesis [Pubmed: 23952606] |
| Journal of Rehabilitation Research & Development | Role of sensory and motor intensity of electrical stimulation on fibroblastic growth factor-2 expression, inflammation, vascularization, and mechanical strength of full-thickness wounds [Pubmed: 23934870] |
| PLoS One.? | Carboxypeptidase E-ΔN, a Neuroprotein Transiently Expressed during Development Protects Embryonic Neurons against Glutamate Neurotoxicity [Pubmed:25426952] |
| Implant Dentistry | Effects of Hemostatic Agents on Fibroblast Cells [Lww:Source] |
| Journal of Materials Chemistry B | bFGF-grafted electrospun fibrous scaffolds via poly(dopamine) for skin wound healing [Rsc:Source] |
| Archives of Animal Nutrition | Effect of silver nanoparticles and hydroxyproline, administered in ovo, on the development of blood vessels and cartilage collagen structure in chicken embryos [Pubmed:25530495] |
| Int J Clin Exp Med. | The effects of self-assembling peptide RADA16 hydrogel on malignant phenotype of human hepatocellular carcinoma cell [PubMed: 26628972] |
| Theriogenology | Evaluation of Changes in Doppler Ultrasonography Indices and Levels of Maternal Serum Angiogenic Factors throughout Pregnancy in Ewes [Science: Article] |
| Tumor Biology | Hypoxia-induced secretion of IL-10 from adipose-derived mesenchymal stem cell promotes growth and cancer stem cell properties of Burkitt lymphoma [PubMed: 26695151] |
| Acta Biomaterialia | Preparation and characterization of pro-angiogenic gel derived from small intestinal submucosa [article:S1742706115301410] |
| 22 | Surface biofunctional drug-loaded electrospun fibrous scaffolds for comprehensive repairing hypertrophic scars [Pubmed:26774564] |
| Genet Mol Res | Changes in growth factor levels in the cerebrospinal fluid of autism patients after transplantation of human umbilical cord blood mononuclear cells and umbilical cord-derived mesenchymal stem cells [Pubmed:27323064] |
| BMC?Neuroscience? | 3-O-Methyldopa inhibits astrocyte-mediated dopaminergic neuroprotective effects of l-DOPA [pubmed:27456338] |
| Medical Science Monitor | 5-Aminolaevulinic Acid-Based Photodynamic Therapy Restrains Pathological Hyperplasia of Fibroblasts [898221] |
| Biomedicine & Pharmacotherapy | FGF-2-mediated FGFR1 signaling in human microvascular endothelial cells is activated by vaccarin to promote angiogenesis [pubmed:28841454] |
| Medical Science Monitor | 5-Aminolaevulinic Acid-Based Photodynamic Therapy Restrains Pathological Hyperplasia of Fibroblasts [pubmed:28052053] |
| International Journal of?Clinical?and Experimental Pathology | Platelet-derived growth factor and stromal cell-derived factor-1 promote the skin wound repairing effect of bone mesenchymal stem cells: a key role of matrix metalloproteinase 1 and collagens [ISSN:1936-2625/IJCEP0058424] |
| Journal of Biomaterials and Tissue Engineering | The Combination of Acellular Porcine Small Intestinal Submucosa (SIS) Cryogel Granules and Adipose Tissue Improves the Survival Ratio of Fat Grafting in Mice [10.1166/jbt.2017.1652] |
| Stem?Cells?and Development | Lower Senescence of Adipose-Derived Stem Cells than Donor-Matched Bone Marrow Stem Cells for Surgical Ventricular Restoration [Pubmed:29630447] |
| Neuroscience?Letters | Increased serum FGF2 levels in first-episode, drug-free patients with schizophrenia [Pubmed: 30172685] |
| Biochemistry and?Cell?Biology | Silencing of Astrocyte elevated gene-1 (AEG-1) inhibits proliferation, migration and invasion, and promotes apoptosis in pancreatic cancer cells [Pubmed: 30359541] |
| Journal of Materials Chemistry B | Gene-activating skin substitute comprised of PLLA/POSS nanofibers and plasmid DNAs encoding ANG and bFGF promotes in vivo revascularization and?… [] |
| BMC?Ophthalmology | HOXA9 is a novel myopia risk gene [Pubmed: 30674274] |
| Applied Materials Today | Immunomodulated electrospun fibrous scaffolds via bFGF camouflage for pelvic regeneration [] |
| International Journal of Ophthalmology | A novel xeno-free culture system for human retinal pigment epithelium cells [Pubmed: 31024807] |
| Open Access Macedonian Journal of Medical Sciences | Low Degree Hyaluronic Acid Crosslinking Inducing the Release of TGF-Β1 in Conditioned Medium of Wharton's Jelly-Derived Stem Cells [] |
| Chemico-Biological Interactions | Bisphenol S triggers the malignancy of hemangioma cells via regulation of basic fibroblast growth factor [Pubmed: 31669319] |
| International Journal of Molecular Sciences | β-Galactosylceramidase Deficiency Causes Bone Marrow Vascular Defects in an Animal Model of Krabbe Disease [Pubmed: 31905906] |
| Molecular Medicine Reports | Long non?coding RNA NORAD regulates angiogenesis of human umbilical vein endothelial cells via miR?590?3p under hypoxic conditions [Pubmed: 32323787] |
| Basic Fibroblast Growth Factor is Involved in Bisphenol S Induced Proliferation of Hemangioma Cells [] | |
| Bioact Mater | New design to remove leukocytes from platelet-rich plasma (PRP) based on cell dimension rather than density [33842739] |
| Acta Biomater | Highly interconnected inverse opal extracellular matrix scaffolds enhance stem cell therapy in limb ischemia [33878473] |
| Materials Science & Engineering C | Double Layer Composite Membrane for Preventing Tendon Adhesion and Promoting Tendon Healing [33812576] |
| Ratlarda deneysel kolon anastomoz ka?a?? modelindeintraperitoneal atorvastatin uygulamas?n?n anastomozka?a?? onar?m? ¨1zerine etkisi [] | |
| World J Hepatol | Leukocyte cell-derived chemotaxin-2 and fibroblast growth factor 21 in alcohol-induced liver cirrhosis [Pubmed:35070009] |
| Biomaterials Advances | Human adipose-derived stem cell-loaded small intestinal submucosa as a bioactive wound dressing for the treatment of diabetic wounds in rats [] |
留言咨詢
