白介素35(IL35)檢測(cè)試劑盒(酶聯(lián)免疫吸附試驗(yàn)法)
ELISA Kit for Interleukin 35 (IL35)
- 編號(hào)SEC008Hu
- 物種Homo sapiens (Human，人) 相同的名稱(chēng)，不同的物種。
- 實(shí)驗(yàn)方法雙抗夾心
- 反應(yīng)時(shí)長(zhǎng)3h
- 檢測(cè)范圍15.6-1,000pg/mL
- 靈敏度最小可檢測(cè)劑量小于等于5.5pg/mL.
- 樣本類(lèi)型serum, plasma, tissue homogenates, cell lysates, cell culture supernates and other biological fluids
- 下載 英文說(shuō)明書(shū) 中文說(shuō)明書(shū)
- 規(guī)格 48T96T 96T*5 96T*10 96T*100
- 價(jià)格 ¥ 2940 ¥ 4200 ¥ 18900 ¥ 35700 ¥ 294000
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產(chǎn)品包裝(模擬)
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產(chǎn)品包裝(模擬)
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實(shí)驗(yàn)結(jié)果圖
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標(biāo)準(zhǔn)曲線(xiàn)圖
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通過(guò)ISO 9001、ISO 13485質(zhì)量體系認(rèn)證
特異性
本試劑盒用于檢測(cè)白介素35(IL35)，經(jīng)檢測(cè)與其它相似物質(zhì)無(wú)明顯交叉反應(yīng)。
由于受到技術(shù)及樣本來(lái)源的限制，不可能完成對(duì)所有相關(guān)或相似物質(zhì)交叉反應(yīng)檢測(cè)，因此本試劑盒有可能與未經(jīng)檢測(cè)的其它物質(zhì)有交叉反應(yīng)。
回收率
分別于定值血清及血漿樣本中加入一定量的白介素35(IL35)（加標(biāo)樣品），重復(fù)測(cè)定并計(jì)算其均值，回收率為測(cè)定值與理論值的比率。
| 樣本 | 回收率范圍(%) | 平均回收率(%) |
| serum(n=5) | 95-105 | 98 |
| EDTA plasma(n=5) | 84-98 | 93 |
| heparin plasma(n=5) | 86-102 | 90 |
精密度
精密度用樣品測(cè)定值的變異系數(shù)CV表示。CV(%) = SD/mean×100
批內(nèi)差：取同批次試劑盒對(duì)低、中、高值定值樣本進(jìn)行定量檢測(cè),每份樣本連續(xù)測(cè)定20 次，分別計(jì)算不同濃度樣本的平均值及SD值。
批間差：選取3個(gè)不同批次的試劑盒分別對(duì)低、中、高值定值樣本進(jìn)行定量測(cè)定，每個(gè)樣本使用同一試劑盒重復(fù)測(cè)定8次，分別計(jì)算不同濃度樣本的平均值及SD值。
批內(nèi)差： CV<10%
批間差： CV<12%
線(xiàn)性
在定值血清及血漿樣本內(nèi)加入適量的白介素35(IL35)，并倍比稀釋成1:2，1:4，1:8，1:16的待測(cè)樣本，線(xiàn)性范圍即為稀釋后樣本中白介素35(IL35)含量的測(cè)定值與理論值的比率。
| 樣本 | 1:2 | 1:4 | 1:8 | 1:16 |
| serum(n=5) | 98-105% | 82-101% | 87-105% | 91-98% |
| EDTA plasma(n=5) | 90-101% | 91-105% | 80-96% | 80-105% |
| heparin plasma(n=5) | 78-97% | 94-101% | 78-97% | 87-102% |
穩(wěn)定性
經(jīng)測(cè)定，試劑盒在有效期內(nèi)按推薦溫度保存，其活性降低率小于5%。
為減小外部因素對(duì)試劑盒破壞前后檢測(cè)值的影響，實(shí)驗(yàn)室的環(huán)境條件需盡量保持一致，尤其是實(shí)驗(yàn)室內(nèi)溫度、濕度及溫育條件。其次由同一實(shí)驗(yàn)員來(lái)進(jìn)行操作可減少人為誤差。
實(shí)驗(yàn)流程
1. 實(shí)驗(yàn)前標(biāo)準(zhǔn)品、試劑及樣本的準(zhǔn)備；
2. 加樣（標(biāo)準(zhǔn)品及樣本）100µL，37°C孵育1小時(shí)；
3. 吸棄，加檢測(cè)溶液A100µL，37°C孵育1小時(shí)；
4. 洗板3次；
5. 加檢測(cè)溶液B100µL，37°C孵育30分鐘；
6. 洗板5次；
7. 加TMB底物90µL，37°C孵育10-20分鐘；
8. 加終止液50µL，立即450nm讀數(shù)。
實(shí)驗(yàn)原理
將IL12A抗體包被于96孔微孔板中，制成固相載體，向微孔中分別加入標(biāo)準(zhǔn)品或標(biāo)本，其中IL35與連接于固相載體上的抗體結(jié)合，然后加入生物素化的IL27B抗體，在將未結(jié)合的生物素化抗體洗凈后，加入HRP標(biāo)記的親和素，再次徹底洗滌后加入TMB底物顯色。TMB在過(guò)氧化物酶的催化下轉(zhuǎn)化成藍(lán)色，并在酸的作用下轉(zhuǎn)化成最終的黃色。顏色的深淺和樣品中的IL35濃度呈正相關(guān)。用酶標(biāo)儀在450nm波長(zhǎng)下測(cè)定吸光度（O.D.值），計(jì)算樣品濃度。
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相關(guān)產(chǎn)品
| 編號(hào) | 適用物種：Homo sapiens (Human，人) | 應(yīng)用(僅供研究使用，不用于臨床診斷！) |
| RPC008Hu01 | 白介素35(IL35)重組蛋白 | Positive Control; Immunogen; SDS-PAGE; WB. |
| APC008Hu01 | 白介素35(IL35)活性蛋白 | Cell?culture;?Activity?Assays. |
| PAC008Hu01 | 白介素35(IL35)多克隆抗體 | WB; IHC; ICC; IP. |
| MAC008Hu25 | 白介素35(IL35)單克隆抗體 | WB; IHC; ICC; IP. |
| MAC008Hu22 | 白介素35(IL35)單克隆抗體 | WB; IHC; ICC; IP. |
| MAC008Hu23 | 白介素35(IL35)單克隆抗體 | WB; IHC; ICC; IP. |
| MAC008Hu24 | 白介素35(IL35)單克隆抗體 | WB; IHC; ICC; IP. |
| MAC008Hu29 | 白介素35(IL35)單克隆抗體 | WB; IHC; ICC; IP. |
| MAC008Hu21 | 白介素35(IL35)單克隆抗體 | WB; IHC; ICC; IP. |
| MAC008Hu28 | 白介素35(IL35)單克隆抗體 | WB; IHC; ICC; IP. |
| MAC008Hu26 | 白介素35(IL35)單克隆抗體 | WB; IHC; ICC; IP. |
| MAC008Hu27 | 白介素35(IL35)單克隆抗體 | WB; IHC; ICC; IP. |
| LAC008Hu72 | 白介素35(IL35)單克隆抗體(生物素標(biāo)記) | WB; IHC; ICC. |
| SEC008Hu | 白介素35(IL35)檢測(cè)試劑盒(酶聯(lián)免疫吸附試驗(yàn)法) | Enzyme-linked immunosorbent assay for Antigen Detection. |
| HEC008Hu | 白介素35(IL35)檢測(cè)試劑盒(酶聯(lián)免疫吸附試驗(yàn)法,高敏型) | Enzyme-linked immunosorbent assay for Antigen Detection. |
| LMC008Hu | 白介素35(IL35)等多因子檢測(cè)試劑盒(流式熒光發(fā)光法) | FLIA Kit for Antigen Detection. |
| PSC008Hu01 | 白介素35(IL35)抗體對(duì) | ELISA; CLIA; ELISPOT; Luminex; Immunochromatography and other Immunoassays. |
| KSC008Hu01 | 白介素35(IL35)檢測(cè)試劑盒DIY材料(酶聯(lián)免疫吸附試驗(yàn)法) | Main materials for "Do It (ELISA Kit) Yourself". |
參考文獻(xiàn)
| 雜志 | 參考文獻(xiàn) |
| Oncology Letters | Aberrant expression of Treg-associated cytokine IL-35 along with IL-10 and TGF-β in acute myeloid leukemia. [PubMed: 22783403] |
| Journal of Maternal-Fetal and Neonatal Medicine | Plasma IL-17, IL-35, interferon-γ, SOCS3 and TGF-β levels in pregnant women with preeclampsia, and their relation with severity of disease [Pubmed: 24175856] |
| Journal of Reproductive Immunology | What is the impact of Th1/Th2 ratio, SOCS3, IL17, and IL35 levels in unexplained infertility? [Pubmed: 24368037] |
| Human Immunology | The imbalanced profile and clinical significance of T helper associated cytokines in bone marrow microenvironment of the patients with acute myeloid leukemia [ScienceDirect: S0198885913005776] |
| Folia Biologica (Praha) | Gene Expression of Subunits of the IL-12 Family Cytokines in moDCs Derived In Vitro from the Cord Blood of Children of Healthy and Allergic Mothers [Pubmed:24785110] |
| Molecular Medicine Reports | Function of interleukin?17 and ?35 in the blood of patients with hepatitis B?related liver cirrhosis [Pubmed:25323532] |
| Rom J Morphol Embryol. | The regular distribution and expression pattern of immunosuppressive cytokine IL-35 in mouse uterus during early pregnancy [Pubmed:25611266] |
| Journal of Immunotherapy | Wogonin Inhibits Tumor-derived Regulatory Molecules by Suppressing STAT3 Signaling to Promote Tumor Immunity [PubMed: 25962106] |
| PLOS ONE | Interleukin 35 Synovial Fluid Levels Are Associated with Disease Activity of Rheumatoid Arthritis [PubMed: 26204444] |
| Rheumatology (Oxford) | Interleukin-35 is upregulated in systemic sclerosis and its serum levels are associated with early disease [PubMed: 26231346] |
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| J Biol Chem | Interleukin-35 Inhibits Endothelial Cell Activation by Suppressing MAPK-AP-1 Pathway [PubMed: 26085094] |
| Br J Haematol | Interleukin 35 may contribute to the loss of immunological self‐tolerance in patients with primary immune thrombocytopenia [PubMed: 25640666] |
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| Clinical?Rheumatology | The clinical utility of serum IL-35 in patients with polymyositis and dermatomyositis [pubmed:27502600] |
| Respiratory?Medicine | Enhanced LPS-induced activation of IL-27 signalling in sarcoidosis [pubmed:27492538] |
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| Reproductive Sciences | Upregulation of Interleukin 35 in Patients With Endometriosis Stimulates Cell Proliferation [pubmed:28659009] |
| EXPERIMENTAL AND THERAPEUTIC?MEDICINE | IL?35 may maintain homeostasis of the immune microenvironment in periodontitis [issn:2542-3975] |
| The Journal of Rheumatology | Synoviocytes-derived Interleukin 35 Potentiates B Cell Response in Patients with Osteoarthritis and Rheumatoid Arthritis [pubmed:29247146] |
| Cellular & Molecular Immunology | Human immunodeficiency virus type-1 induces a regulatory B cell-like phenotype in vitro [pubmed:28713164] |
| Molecular?Medicine?Reports | Lower level of IL?35 and its reduced inhibition in Th17 cells in patients with bone marrow mononuclear cells Coombs test?positive hemocytopenia. [pubmed:29257310] |
| Asian pacific journal of allergy and immunology | Evaluation of serum interleukin- 35 level in children with persistent asthma. [pubmed:27996283] |
| PLoS One | Interleukin-35 mitigates the function of murine transplanted islet cells via regulation of Treg/Th17 ratio [pubmed:29236782] |
| Hepatology Communications | Significant association between FOXP3 gene polymorphism and steroid-resistant acute rejection in living donor liver transplantation [10.1002/hep4.1052] |
| International Journal of Clinical and Experimental Medicine | Clinical significance of IL-35 expression in the progression of clear cell renal cell carcinoma [ISSN:1940-5901] |
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| Annals of Rheumatic Diseases? | AB0734 Serum il-35 levels in systemic sclerosis and relationship with clinical features [10.1136:annrheumdis-2018-eular.2605] |
| Tohoku?Journal of Experimental?Medicine | Interleukin-35 as a New Biomarker of Renal Involvement in Lupus Nephritis Patients [Pubmed:29576585] |
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| Oncotarget | Pro-tumoral immune cell alterations in wild type and Shb-deficient mice in response to 4T1 breast carcinomas [Pubmed:29721156] |
| Journal of Clinical Rheumatology | Serum Interleukin 35 Levels in Systemic Sclerosis and Relationship With Clinical Features. [Pubmed: 30431486] |
| Oncology Letters | Interleukin?35 is associated with the tumorigenesis and progression of prostate cancer [] |
| Journal of Clinical Investigation | Plasmacytoid dendritic cells protect against immune-mediated acute liver injury via IL-35 [Pubmed: 31264967] |
| Cells | CD4+ FOXP3+ T Cells in Rheumatoid Arthritis Bone Marrow Are Partially Impaired [Pubmed: 32111105] |
| Cytokine | Interleukin-35 in idiopathic inflammatory myopathies [Pubmed: 33128920] |
| FEBS Open Bio | IL35 predicts prognosis in gastric cancer and is associated with angiogenesis by altering TIMP1, PAI1, and IGFBP1 [Pubmed: 33064893] |
| Cancer Cell International | Interleukin-35 promotes progression of prostate cancer and inhibits anti-tumour immunity [Pubmed: 33041668] |
| Journal of Neuroinflammation | Interleukin-9 regulates macrophage activation in the progressive multiple sclerosis brain [Pubmed: 32375811] |
| Int Immunopharmacol | Association of IL-35 expression and gene polymorphisms in rheumatoid arthritis [33307515] |
| Archives of Medical Science | Decreased interleukin-35 levels and CD4+ EBI3+ T cells in patients with type 1 diabetes and the effects of the antibody against CD20 (rituximab) [33488880] |
| Asia Pac Allergy | Anti-inflammatory roles of interleukin-35 in the pathogenesis of Japanese cedar pollinosis [34386410] |
| Int Immunopharmacol | Pentosan Polysulfate Sodium augments the therapeutic effect of 5-Aminosalicylic Acid in DSS colitis model; the role of IL-35 expression [Pubmed:35247859] |
| Biology and Medical Researches | Association of three single nucleotide polymorphisms of interleukin-35 for detection and prediction of women with breast cancer in Erbil city [] |
| Research Square | Analysis of IL-10 and IL-35 in DPP-4 inhibitor-related bullous pemphigoid [] |
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