Ⅰ型前膠原羧基端原肽(PICP)檢測試劑盒(酶聯(lián)免疫吸附試驗法)
ELISA Kit for Procollagen I C-Terminal Propeptide (PICP)
P1CP; C-Propeptide Of Type I Procollagen; Procollagen I Carboxy Terminal Propeptide
- 編號SEA570Hu
- 物種Homo sapiens (Human，人) 相同的名稱，不同的物種。
- 實驗方法雙抗夾心
- 反應時長3h
- 檢測范圍0.312-20ng/mL
- 靈敏度最小可檢測劑量小于等于0.129ng/mL.
- 樣本類型serum, plasma, tissue homogenates, cell lysates, cell culture supernates and other biological fluids
- 下載 英文說明書 中文說明書
- 規(guī)格 48T96T 96T*5 96T*10 96T*100
- 價格 ¥ 2646 ¥ 3780 ¥ 17010 ¥ 32130 ¥ 264600
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產(chǎn)品包裝(模擬)
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產(chǎn)品包裝(模擬)
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實驗結果圖
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標準曲線圖
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通過ISO 9001、ISO 13485質量體系認證
特異性
本試劑盒用于檢測Ⅰ型前膠原羧基端原肽(PICP)，經(jīng)檢測與其它相似物質無明顯交叉反應。
由于受到技術及樣本來源的限制，不可能完成對所有相關或相似物質交叉反應檢測，因此本試劑盒有可能與未經(jīng)檢測的其它物質有交叉反應。
回收率
分別于定值血清及血漿樣本中加入一定量的Ⅰ型前膠原羧基端原肽(PICP)（加標樣品），重復測定并計算其均值，回收率為測定值與理論值的比率。
| 樣本 | 回收率范圍(%) | 平均回收率(%) |
| serum(n=5) | 92-101 | 97 |
| EDTA plasma(n=5) | 81-101 | 91 |
| heparin plasma(n=5) | 90-103 | 95 |
精密度
精密度用樣品測定值的變異系數(shù)CV表示。CV(%) = SD/mean×100
批內(nèi)差：取同批次試劑盒對低、中、高值定值樣本進行定量檢測,每份樣本連續(xù)測定20 次，分別計算不同濃度樣本的平均值及SD值。
批間差：選取3個不同批次的試劑盒分別對低、中、高值定值樣本進行定量測定，每個樣本使用同一試劑盒重復測定8次，分別計算不同濃度樣本的平均值及SD值。
批內(nèi)差： CV<10%
批間差： CV<12%
線性
在定值血清及血漿樣本內(nèi)加入適量的Ⅰ型前膠原羧基端原肽(PICP)，并倍比稀釋成1:2，1:4，1:8，1:16的待測樣本，線性范圍即為稀釋后樣本中Ⅰ型前膠原羧基端原肽(PICP)含量的測定值與理論值的比率。
| 樣本 | 1:2 | 1:4 | 1:8 | 1:16 |
| serum(n=5) | 81-94% | 89-102% | 79-102% | 80-90% |
| EDTA plasma(n=5) | 84-93% | 89-99% | 79-95% | 79-91% |
| heparin plasma(n=5) | 82-91% | 83-95% | 78-102% | 83-101% |
穩(wěn)定性
經(jīng)測定，試劑盒在有效期內(nèi)按推薦溫度保存，其活性降低率小于5%。
為減小外部因素對試劑盒破壞前后檢測值的影響，實驗室的環(huán)境條件需盡量保持一致，尤其是實驗室內(nèi)溫度、濕度及溫育條件。其次由同一實驗員來進行操作可減少人為誤差。
實驗流程
1. 實驗前標準品、試劑及樣本的準備；
2. 加樣（標準品及樣本）100µL，37°C孵育1小時；
3. 吸棄，加檢測溶液A100µL，37°C孵育1小時；
4. 洗板3次；
5. 加檢測溶液B100µL，37°C孵育30分鐘；
6. 洗板5次；
7. 加TMB底物90µL，37°C孵育10-20分鐘；
8. 加終止液50µL，立即450nm讀數(shù)。
實驗原理
將Ⅰ型前膠原羧基端原肽(PICP)抗體包被于96孔微孔板中，制成固相載體，向微孔中分別加入標準品或標本，其中的Ⅰ型前膠原羧基端原肽(PICP)與連接于固相載體上的抗體結合，然后加入生物素化的Ⅰ型前膠原羧基端原肽(PICP)抗體，將未結合的生物素化抗體洗凈后，加入HRP標記的親和素，再次徹底洗滌后加入TMB底物顯色。TMB在過氧化物酶的催化下轉化成藍色，并在酸的作用下轉化成最終的黃色。顏色的深淺和樣品中的Ⅰ型前膠原羧基端原肽(PICP)呈正相關。用酶標儀在450nm波長下測定吸光度(O.D.值)，計算樣品濃度。
相關產(chǎn)品
| 編號 | 適用物種：Homo sapiens (Human，人) | 應用(僅供研究使用，不用于臨床診斷！) |
| RPA570Hu01 | Ⅰ型前膠原羧基端原肽(PICP)重組蛋白 | Positive Control; Immunogen; SDS-PAGE; WB. |
| EPA570Hu61 | Ⅰ型前膠原羧基端原肽(PICP)真核蛋白 | Positive Control; Immunogen; SDS-PAGE; WB. |
| APA570Hu61 | Ⅰ型前膠原羧基端原肽(PICP)活性蛋白 | Cell?culture;?Activity?Assays. |
| CPA570Hu22 | Ⅰ型前膠原羧基端原肽(PICP)卵白蛋白偶聯(lián)物 | Immunogen; SDS-PAGE; WB. |
| CPA570Hu21 | Ⅰ型前膠原羧基端原肽(PICP)卵白蛋白偶聯(lián)物 | Immunogen; SDS-PAGE; WB. |
| PAA570Hu01 | Ⅰ型前膠原羧基端原肽(PICP)多克隆抗體 | WB; IHC; ICC; IP. |
| PAA570Hu08 | Ⅰ型前膠原羧基端原肽(PICP)多克隆抗體 | WB; IHC; ICC; IP. |
| SEA570Hu | Ⅰ型前膠原羧基端原肽(PICP)檢測試劑盒(酶聯(lián)免疫吸附試驗法) | Enzyme-linked immunosorbent assay for Antigen Detection. |
| SCA570Hu | Ⅰ型前膠原羧基端原肽(PICP)檢測試劑盒(化學發(fā)光免疫分析法) | Chemiluminescent immunoassay for Antigen Detection. |
| LMA570Hu | Ⅰ型前膠原羧基端原肽(PICP)等多因子檢測試劑盒(流式熒光發(fā)光法) | FLIA Kit for Antigen Detection. |
參考文獻
| 雜志 | 參考文獻 |
| Nutrition | Dyslipidemic high-fat diet affects adversely bone metabolism in mice associated with impaired antioxidant capacity [ScienceDirect: S0899900709004717] |
| American Heart Journal | Serum markers of deranged myocardial collagen turnover: their relation to malignant ventricular arrhythmias in cardioverter-defibrillator recipients with heart failure. [PubMed: 23067911] |
| Journal of Hypertension | Circulating biomarkers of collagen metabolism in arterial hypertension: relevance of target organ damage [Pubmed: 23615327] |
| Pancreatology | Cdkn1a is a key mediator of rat pancreatic stellate cell senescence [Pubmed: 23719597] |
| The American Journal of Cardiology | Relation of burden of myocardial fibrosis to malignant ventricular arrhythmias and outcomes in Fabry disease [Pubmed:25073565] |
| PLoS One. | An NMR Metabolomic Study on the Effect of Alendronate in Ovariectomized Mice [Pubmed:25184758] |
| Journal of Bone and Mineral Research | The Anti-Osteoanabolic Function of Sclerostin is Blunted in Mice Carrying a High Bone Mass Mutation of Lrp5 [Pubmed:25640331] |
| Bone | The active role of osteoporosis in the interaction between osteoblasts and bone metastases [PubMed: 26057367] |
| J Nutr Biochem | Consumption of vitamin D2 [PubMed: 25792284] |
| Bibliothekssystem Universit?t Hamburg | Bilaterale Kommunikation zwischen Osteoblasten und Osteoklasten [volltexte:2016] |
| Inflammation Research | Fibrosis of extracellular matrix is related to the duration of the disease but is unrelated to the dynamics of collagen metabolism in dilated cardiomyopathy [pubmed:27516211] |
| Heart & Vessels | Left ventricular reverse remodeling is not related to biopsy-detected extracellular matrix fibrosis and serum markers of fibrosis in dilated cardiomyopathy, regardless of the definition used for LVRR [pubmed:28004175] |
| Scandinavian?Journal?of?Rheumatology | The?association?between?insulin-like?growth?factor?1?(IGF-1),?IGF-binding?proteins?(IGFBPs), and the?carboxyterminal?propeptide?of?type?I?procollagen?(PICP) in pre- and postmenopausal women with rheumatoid arthritis. [pubmed:27775453] |
| Allergy?and?Asthma?Proceedings | Effect?of?montelukast?on?markers?of?airway remodeling?in?children?withasthma. [pubmed:27657514] |
| Scandinavian Journal of Rheumatology | The association between insulin-like growth factor 1 (IGF-1), IGF-binding proteins (IGFBPs), and the carboxyterminal propeptide of type I procollagen (PICP) in pre- and postmenopausal women with rheumatoid arthritis [loi:irhe20] |
| Conditional Mouse Models Support the Role of SLC39A14 (ZIP14) in Hyperostosis Cranialis Interna and in Bone Homeostasis [ISBN: 978-94-6299-582-6] | |
| MOLECULAR MEDICINE REPORTS | Endothelial?to?mesenchymal transition in human idiopathic dilated cardiomyopathy [pubmed:29115553] |
| Pediatrics and Neonatology | Age-related changes in biochemical bone profile in thalassemic children [pubmed:28967496] |
| Heart and Vessels | Left ventricular reverse remodeling is not related to biopsy?detected extracellular matrix fibrosis and serum markers of fibrosis in dilated cardiomyopathy, regardless of the definition used for LVRR [10.1007/s00380-016-0930-y] |
| Journal of Bone and Mineral Research | The Lrp4 R1170Q homozygous knock‐in mouse recapitulates the bone phenotype of sclerosteosis in humans [pubmed:28477420] |
| Cytokine | 12-month patterns of serum markers of collagen synthesis, transforming growth factor and connective tissue growth factor are similar in new-onset and chronic dilated cardiomyopathy in patients both with and without cardiac fibrosis. [pubmed:28460256] |
| Advanced Healthcare Materials | Prognostic value of fibrosis-related markers in dilated cardiomyopathy: A link between osteopontin and cardiovascular events. [pubmed:29120858] |
| Journal of?Bone?and?Mineral?Metabolism | Mutations That Alter the Carboxy‐Terminal‐Propeptide Cleavage Site of the Chains of Type I Procollagen Are Associated With a Unique Osteogenesis Imperfecta?… [Pubmed:29669177] |
| Cardiovascular Journal of Africa | Assessment of myocardial fibrosis by late gadolinium enhancement imaging and biomarkers of collagen metabolism in chronic rheumatic mitral regurgitation. [Pubmed:29443354] |
| Journal?of?Cellular?Physiology | DNA methylation regulates α‐smooth muscle actin expression during cardiac fibroblast differentiation [Pubmed: 30362530] |
| International Journal of Chronic Obstructive Pulmonary Disease | Altered serum levels of type I collagen turnover indicators accompanied by IL-6 and IL-8 release in stable COPD [Pubmed: 30655663] |
| Scientific Reports | Low fibrosis biomarker levels predict cardiac resynchronization therapy response [Pubmed: 30988339] |
| Bone | Agonist-induced activation of the S1P receptor 2 constitutes a novel osteoanabolic therapy for the treatment of osteoporosis in mice [Pubmed: 31028959] |
| PLoS One | Deficiency of sphingosine-1-phosphate receptor 3 does not affect the skeletal phenotype of mice lacking sphingosine-1-phosphate lyase [Pubmed: 31314788] |
| Bone | Mice lacking plastin-3 display a specific defect of cortical bone acquisition [Pubmed: 31678489] |
| Journal of Diabetes Research | Type 2 Diabetes Mellitus and Chronic Heart Failure with Midrange and Preserved Ejection Fraction: A Focus on Serum Biomarkers of Fibrosis [Pubmed: 33224989] |
| JOURNAL OF BONE AND MINERAL RESEARCH | Piezo1 Inactivation in Chondrocytes Impairs Trabecular Bone Formation [Pubmed: 33180356] |
| Microbiome changes in patients with chronic heart failure with preserved ejection fraction correlate with fibrosis markers: Description of a Russian cohort [] | |
| Ассоциации толщины эпикардиального жира и циркулирующих маркеров фиброза миокарда у пациентов с инфарктом миокарда [] | |
| Russian Journal of Cardiology | Early diagnosis of myocardial fibrosis in patients with epicardial obesity [] |
| J Diabetes Res | Association between Markers of Fibrosis and Heart Failure Incidence in Patients with Type 2 Diabetes Mellitus [34778465] |
| Clinical, Cosmetic and Investigational Dermatology | Extra Cellular Matrix Deposition and Assembly in Dermis Spheroids [34321901] |
| Scandinavian Cardiovascular Journal | Dual integrin αvβ3 and αvβ5 blockade attenuates cardiac dysfunction by reducing fibrosis in a rat model of doxorubicin-induced cardiomyopathy [34296634] |
| Eur J Appl Physiol | Effects of 8-week increment aerobic exercise program on bone metabolism and body composition in young non-athletes [Pubmed:35141785] |
| J. Clin. Med | Simple Predictors for Cardiac Fibrosis in Patients with Type 2 Diabetes Mellitus: The Role of Circulating Biomarkers and Pulse Wave Velocity [Pubmed:35628969] |
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