白介素12(IL12)檢測試劑盒(酶聯(lián)免疫吸附試驗法)
ELISA Kit for Interleukin 12 (IL12)
p70
- 編號SEA111Ga
- 物種Chicken (Gallus，雞) 相同的名稱，不同的物種。
- 實驗方法雙抗夾心
- 反應(yīng)時長3h
- 檢測范圍15.6-1000pg/mL
- 靈敏度最小可檢測劑量小于等于6.1pg/mL.
- 樣本類型serum, plasma, tissue homogenates, cell lysates, cell culture supernates and other biological fluids
- 下載 英文說明書 中文說明書
- 規(guī)格 48T96T 96T*5 96T*10 96T*100
- 價格 ¥ 2873 ¥ 4104 ¥ 18468 ¥ 34884 ¥ 287280
- 欲了解實際交易價格和更多情況，請與當(dāng)?shù)亟?jīng)銷商聯(lián)系!
-
產(chǎn)品包裝(模擬)
-
產(chǎn)品包裝(模擬)
-
實驗結(jié)果圖
-
標(biāo)準(zhǔn)曲線圖
-
通過ISO 9001、ISO 13485質(zhì)量體系認(rèn)證
特異性
本試劑盒用于檢測白介素12(IL12)，經(jīng)檢測與其它相似物質(zhì)無明顯交叉反應(yīng)。
由于受到技術(shù)及樣本來源的限制，不可能完成對所有相關(guān)或相似物質(zhì)交叉反應(yīng)檢測，因此本試劑盒有可能與未經(jīng)檢測的其它物質(zhì)有交叉反應(yīng)。
回收率
分別于定值血清及血漿樣本中加入一定量的白介素12(IL12)（加標(biāo)樣品），重復(fù)測定并計算其均值，回收率為測定值與理論值的比率。
| 樣本 | 回收率范圍(%) | 平均回收率(%) |
| serum(n=5) | 78-101 | 82 |
| EDTA plasma(n=5) | 79-101 | 90 |
| heparin plasma(n=5) | 79-94 | 88 |
精密度
精密度用樣品測定值的變異系數(shù)CV表示。CV(%) = SD/mean×100
批內(nèi)差：取同批次試劑盒對低、中、高值定值樣本進(jìn)行定量檢測,每份樣本連續(xù)測定20 次，分別計算不同濃度樣本的平均值及SD值。
批間差：選取3個不同批次的試劑盒分別對低、中、高值定值樣本進(jìn)行定量測定，每個樣本使用同一試劑盒重復(fù)測定8次，分別計算不同濃度樣本的平均值及SD值。
批內(nèi)差： CV<10%
批間差： CV<12%
線性
在定值血清及血漿樣本內(nèi)加入適量的白介素12(IL12)，并倍比稀釋成1:2，1:4，1:8，1:16的待測樣本，線性范圍即為稀釋后樣本中白介素12(IL12)含量的測定值與理論值的比率。
| 樣本 | 1:2 | 1:4 | 1:8 | 1:16 |
| serum(n=5) | 89-103% | 94-101% | 86-96% | 95-103% |
| EDTA plasma(n=5) | 79-99% | 90-98% | 79-98% | 95-104% |
| heparin plasma(n=5) | 79-90% | 88-103% | 87-94% | 78-102% |
穩(wěn)定性
經(jīng)測定，試劑盒在有效期內(nèi)按推薦溫度保存，其活性降低率小于5%。
為減小外部因素對試劑盒破壞前后檢測值的影響，實驗室的環(huán)境條件需盡量保持一致，尤其是實驗室內(nèi)溫度、濕度及溫育條件。其次由同一實驗員來進(jìn)行操作可減少人為誤差。
實驗流程
1. 實驗前標(biāo)準(zhǔn)品、試劑及樣本的準(zhǔn)備；
2. 加樣（標(biāo)準(zhǔn)品及樣本）100µL，37°C孵育1小時；
3. 吸棄，加檢測溶液A100µL，37°C孵育1小時；
4. 洗板3次；
5. 加檢測溶液B100µL，37°C孵育30分鐘；
6. 洗板5次；
7. 加TMB底物90µL，37°C孵育10-20分鐘；
8. 加終止液50µL，立即450nm讀數(shù)。
實驗原理
將IL12A抗體包被于96孔微孔板中，制成固相載體，向微孔中分別加入標(biāo)準(zhǔn)品或標(biāo)本，其中IL12與連接于固相載體上的抗體結(jié)合，然后加入生物素化的IL12B抗體，在將未結(jié)合的生物素化抗體洗凈后，加入HRP標(biāo)記的親和素，再次徹底洗滌后加入TMB底物顯色。TMB在過氧化物酶的催化下轉(zhuǎn)化成藍(lán)色，并在酸的作用下轉(zhuǎn)化成最終的黃色。顏色的深淺和樣品中的IL12濃度呈正相關(guān)。用酶標(biāo)儀在450nm波長下測定吸光度（O.D.值），計算樣品濃度。
增值服務(wù)
相關(guān)產(chǎn)品
| 編號 | 適用物種：Chicken (Gallus，雞) | 應(yīng)用(僅供研究使用，不用于臨床診斷！) |
| RPA111Ga01 | 白介素12(IL12)重組蛋白 | Positive Control; Immunogen; SDS-PAGE; WB. |
| SEA111Ga | 白介素12(IL12)檢測試劑盒(酶聯(lián)免疫吸附試驗法) | Enzyme-linked immunosorbent assay for Antigen Detection. |
| LMA111Ga | 白介素12(IL12)等多因子檢測試劑盒(流式熒光發(fā)光法) | FLIA Kit for Antigen Detection. |
參考文獻(xiàn)
| 雜志 | 參考文獻(xiàn) |
| Journal?of?Virology | Characterization?of?Simian?Immunodeficiency?Virus?Variants?Anatomically?Compartmentalized?inPlasma?and?Milk?in?Chronically?Infected?African?Green?Monkeys. [pubmed:] |
| Cancer?Science | A Novel Potential Strategy for Enhancing Antigen Presentation of Human Hepatocellular Carcinoma Cells Propagated ex-vivo Using a Ribonuclease Enzyme?… [Scholarena:Source] |
| International Immunopharmacology | Switched memory B cells promote alveolar bone damage during periodontitis: An adoptive transfer experiment [Pubmed:30015235] |
| Oncology?Letters | Antitumor efficacy of the Runx2?dendritic cell vaccine in triple?negative breast cancer in vitro [Pubmed:30127867] |
| Neuroscience | The P2X7 receptor, cathepsin S and fractalkine in the trigeminal subnucleus caudalis signal persistent hypernociception in temporomandibular rat joints [Pubmed: 30248434] |
| Cancer?Biomarkers | Soluble LAG3 acts as a potential prognostic marker of gastric cancer and its positive correlation with CD8+ T cell frequency and secretion of IL-12 and INF-γ in?… [Pubmed: 30223387] |
| International?Journal?of?Clinical?and?Experimental?Medicine | The relationship between autophagy and apoptosis of lamina propria dendritic cells from the small intestine on the immunodynamics of sepsis mice [] |
| ВЛИЯНИЕ ДНК МЕТИЛТРАНСФЕРАЗЫ 3А И ГИСТОНДЕАЦЕТИЛАЗЫ НА СОДЕРЖАНИЕ В МОНОНУКЛЕАРНЫХ КЛЕТКАХ ЦЕЛЬНОЙ КРОВИ?… [] | |
| Experimental and Therapeutic Medicine | RBP and Alb in neonatal hydronephrosis and its association with IL-12 levels in pregnant women before delivery [] |
| Chemosphere | Atmospheric H2S triggers immune damage by activating the TLR-7/MyD88/NF-κB pathway and NLRP3 inflammasome in broiler thymus [] |
| Infection and Drug Resistance | Disseminated And Infection Accompanied Sweet's Syndrome In A Patient With Anti-Interferon-γ Autoantibodies: A Case Report. [Pubmed: 31632104] |
| Biomed Pharmacother | Suppression of Fpr2 expression protects against endotoxin-induced acute lung injury by interacting with Nrf2-regulated TAK1 activation [Pubmed: 32106380] |
| Silkworm pupa oil attenuates acetaminophen‐induced acute liver injury by inhibiting oxidative stress‐mediated NF‐κB signaling [Pubmed: 31993149] | |
| J Vet Med Sci | Innate immune response of mammary gland induced by intramammary infusion of Bifidobacterium breve in lactating dairy cows [Pubmed: 33071254] |
| Bat? Karadeniz T?p Dergisi | Vasküler Demans Modelinde ?evresel Zenginle?tirmenin Depresyon Benzeri Davran??, Kortikal ve Hipokampal BDNF ve IL-1β Seviyeleri üzerine Etkisi [] |
| Evaluation of lipopolysaccharide-induced acute lung injury attenuation in mice by Glycyrrhiza glabra [] | |
| Life Sci | BBG enhances OLT1177-induced NLRP3 inflammasome inactivation by targeting P2X7R/NLRP3 and MyD88/NF-|êB signaling in DSS-induced colitis in rats [33548287] |
| IUBMB Life | HOXA5 induces M2 macrophage polarization to attenuate carotid atherosclerosis by activating MED1 [34117711] |
| Int J Biol Macromol | Establishing novel roles of bifidocin LHA, antibacterial, antibiofilm and immunomodulator against Pseudomonas aeruginosa corneal infection model [34171261] |
| Am J Transl Res | Combined treatment with epigenetic agents enhances anti-tumor activity of T cells by upregulating the ACRBP expression in hepatocellular carcinoma [34377237] |
| J Periodontol | Cytokine profile in serum and gingival crevicular fluid of children with inflammatory bowel disease: A case‐control study [34730850] |
| Biomedicines | Exploring Epithelial–Mesenchymal Transition Signals in Endometriosis Diagnosis and In Vitro Fertilization Outcomes [34829910] |
| Journal of Nanobiotechnology | Biomimetic GBM-targeted drug delivery system boosting ferroptosis for immunotherapy of orthotopic drug-resistant GBM [Pubmed:35351131] |
| J Nanobiotechnology | Cyclosporine A loaded brain targeting nanoparticle to treat cerebral ischemia/reperfusion injury in mice [Pubmed:35658867] |
| Biomedicines | Deletion of Toxoplasma Rhoptry Protein 38 (PruΔrop38) as a Vaccine Candidate for Toxoplasmosis in a Murine Model [Pubmed:35740356] |
