甲狀腺素(T4)多克隆抗體(異硫氰酸熒光素標記)
FITC-Linked Polyclonal Antibody to Thyroxine (T4)
3,5,3',5'-Tetraiodothyronine; L-Thyroxine; Levothyroxine
- 編號LAA452Ge81
- 物種Pan-species (General,通用) 相同的名稱,不同的物種。
- 來源抗體標記
- Ig類型 IgG
- 純化方式抗原特異性親和純化
- 標記物熒光素
- 原抗體 PAA452Ge01-甲狀腺素(T4)多克隆抗體
- 緩沖液成份磷酸鹽緩沖液(pH7.4,含有0.02% NaN3和50%甘油)
- 性狀液體
- 濃度500μg/mL
- 且適物種Pan-species (General,通用)
- 應用WB; IHC; ICC; IF.
如果抗體需用于流式細胞術,請參見流式抗體。 - 下載 英文說明書 中文說明書
- 規(guī)格 20μl100μl 200μl 1ml 10ml
- 價格 ¥ 801 ¥ 1869 ¥ 2670 ¥ 6675 ¥ 26700
- 欲了解實際交易價格和更多情況,請與當地經銷商聯(lián)系!
特異性
該抗體是針對T4的兔多克隆抗體。在免疫組織化學染色和免疫印跡實驗中能識別T4。
用法
Western blotting: 0.5-2ug/ml
Immunocytochemistry in formalin fixed cells: 5-20ug/ml
Immunohistochemistry in formalin fixed frozen section: 5-20ug/ml
Immunohistochemistry in paraffin section: 5-20ug/ml
Enzyme-linked Immunosorbent Assay: 0.05-2ug/ml
Optimal working dilutions must be determined by end user.
儲存
經常使用則4°C保存。-20°C保存不超過兩年。避免反復凍融。
穩(wěn)定性
熱穩(wěn)定性以損失率顯示。損失率是由加速降解試驗決定,具體方法如下:在37°C孵育48小時,沒有顯著的降解或者沉淀產生。保質期內,在適當的條件下存儲,損失率低于5%。
贈品
相關產品
編號 | 適用物種:Pan-species (General,通用) | 應用(僅供研究使用,不用于臨床診斷!) |
CPA452Ge11 | 甲狀腺素(T4)牛血清白蛋白偶聯(lián)物 | Immunogen; SDS-PAGE; WB. |
CPA452Ge21 | 甲狀腺素(T4)卵白蛋白偶聯(lián)物 | Immunogen; SDS-PAGE; WB. |
PAA452Ge01 | 甲狀腺素(T4)多克隆抗體 | ELISA, CLIA. / IHC-Fr, ICC, IP (predicted). |
LAA452Ge81 | 甲狀腺素(T4)多克隆抗體(異硫氰酸熒光素標記) | WB; IHC; ICC; IF. |
MAA452Ge28 | 甲狀腺素(T4)單克隆抗體 | ELISA, CLIA. / IHC-Fr, ICC, IP (predicted). |
MAA452Ge25 | 甲狀腺素(T4)單克隆抗體 | ELISA, CLIA. / IHC-Fr, ICC, IP (predicted). |
MAA452Ge23 | 甲狀腺素(T4)單克隆抗體 | ELISA, CLIA. / IHC-Fr, ICC, IP (predicted). |
MAA452Ge22 | 甲狀腺素(T4)單克隆抗體 | ELISA, CLIA. / IHC-Fr, ICC, IP (predicted). |
MAA452Ge21 | 甲狀腺素(T4)單克隆抗體 | ELISA, CLIA. / IHC-Fr, ICC, IP (predicted). |
CEA452Ge | 甲狀腺素(T4)檢測試劑盒(酶聯(lián)免疫吸附試驗法) | Enzyme-linked immunosorbent assay for Antigen Detection. |
IEA452Ge | 甲狀腺素(T4)檢測試劑盒(酶聯(lián)免疫吸附試驗法,快捷型) | Enzyme-linked immunosorbent assay for Antigen Detection. |
MEA452Ge | 甲狀腺素(T4)檢測試劑盒(酶聯(lián)免疫吸附試驗法,小樣本) | Enzyme-linked immunosorbent assay for Antigen Detection. |
CCA452Ge | 甲狀腺素(T4)檢測試劑盒(化學發(fā)光免疫分析法) | Chemiluminescent immunoassay for Antigen Detection. |
LMA452Ge | 甲狀腺素(T4)等多因子檢測試劑盒(流式熒光發(fā)光法) | FLIA Kit for Antigen Detection. |
KSA452Ge11 | 甲狀腺素(T4)檢測試劑盒DIY材料(酶聯(lián)免疫吸附試驗法) | 自己動手制作ELISA試劑盒的主要材料 |
參考文獻
雜志 | 參考文獻 |
Aquatic Toxicology | Bioconcentration and metabolism of decabromodiphenyl ether (BDE-209) result in thyroid endocrine disruption in zebrafish larvae [ScienceDirect: S0166445X12000215] |
Aquatic Toxicology | Exposure of zebrafish embryos/larvae to TDCPP alters concentrations of thyroid hormones and transcriptions of genes involved in the hypothalamic–pituitary–thyroid axis [PubMed: 23220413] |
Life Science Journal | Can Nigella Sativa oil (NSO) reverse hypothyroid status induced by PTU in rat? biochemical and histological studies [Lifesciencesite: Source] |
Aquat Toxicol | Thyroid endocrine system disruption by pentachlorophenol: an in vitro and in vivo assay. [ScienceDirect: S0166445X13002129] |
Aquatic Toxicology | Impact of co-exposure with lead and decabromodiphenyl ether (BDE-209) on thyroid function in zebrafish larvae [Pubmed:25456233] |
Chemosphere | Exposure to2,2′,4,4′-tetrabromodiphenyl ether (BDE-47) alters thyroid hormone levels and thyroid hormone-reCavia (Guinea pig )lated gene transcription in manila clam?Ruditapes philippinarum [science:S0045653516302016] |
Frontiers in cellular neuroscience | Elevated Thyroid Peroxidase Antibody Increases Risk of Post-partum Depression by Decreasing Prefrontal Cortex BDNF and 5-HT Levels in Mice [pubmed:28119573] |
Toxicological?Sciences | Exposure of pregnant mice to perfluorobutanesulfonate causes hypothyroxinemia and developmental abnormalities in female offspring [pubmed:27803384] |
Sci Rep | Exposure of pregnant mice to triclosan impairs placental development and nutrient transport. [pubmed:28322267] |
Aquat Toxicol. | Developmental toxicity and thyroid hormone-disrupting effects of 2,4-dichloro-6-nitrophenol in Chinese rare minnow (Gobiocypris rarus). [pubmed:28187359] |
Bulletin of the World Health Organization | Endemic goitre in the Sudan despite longstanding programmes for the control of iodine deficiency disorders [handle:123456789] |
Biological Rhythm Research | Age-related and seasonal variations in plasma uncarboxylated osteocalcin in male Murrah buffaloes [09291016.2016.1275393] |
Toxicological Sciences | Exposure of Pregnant Mice to Triclosan Causes Insulin Resistance via Thyroxine Reduction [pubmed:28973666] |
Environmental Pollution | Transgenerational endocrine disruption and neurotoxicity in zebrafish larvae after parental exposure to binary mixtures of decabromodiphenyl ether (BDE-209) and lead [10.1016/j.envpol.2017.06.053] |
Sciencepaper online | 四溴雙酚A對菲律賓蛤仔的生長抑制效應機理Growth inhibition Effects of TBBPA on juvenile manila clam Ruditapes philippinarum [5790267088906950000] |
Tropical Journal of Pharmaceutical Research | Neuroprotective effects of thymoquinone against cerebellar histopathological changes in propylthiouracil-induced hypothyroidism in adult rats [10.4314/tjpr.v16i5.9] |
s-space | Thyroid disrupting effects and associated mechanisms of TCPP in GH3 cell line and zebrafish (Danio rerio) larva and adult [10371/137708] |
Environmental Pollution | Time-dependent inhibitory effects of Tris(1, 3-dichloro-2-propyl) phosphate on growth and transcription of genes involved in the GH/IGF axis, but not the HPT axis, in female zebrafish. [pubmed:28624628] |
Environmental pollution | Stereoselective degradation and thyroid endocrine disruption of lambda-cyhalothrin in lizards (Eremias argus) following oral exposure [pubmed:28970022] |
Environmental?Science?& Technology | Thyroid hormone-disrupting potentials of major benzophenones in two cell lines (GH3 and FRTL-5) and embryo-larval zebrafish [Pubmed:29995391] |
Environmental Pollution | Prenatal exposure to polychlorinated biphenyl and umbilical cord hormones and birth outcomes in an island population [Pubmed:29525625] |
Toxicological?Sciences | Impact of Perfluorooctane Sulfonate on Reproductive Ability of Female Mice through Suppression of Estrogen Receptor a-Activated Kisspeptin Neurons [10.1093:toxsci] |
Frontiers in Molecular Neuroscience | Impact of Triclosan on Female Reproduction through Reducing Thyroid Hormones to Suppress Hypothalamic Kisspeptin Neurons in Mice [Pubmed:29403355] |
Drug?metabolism and disposition | Activation of 5-HT1A receptors in the hypothalamic paraventricular nuclei (PVN) negatively regulates cytochrome P450 expression and activity in rat liver [Pubmed:29555828] |
International Journal of Endocrinology | Histopathological Characterization and Whole Exome Sequencing of Ectopic Thyroid: Fetal Architecture in a Functional Ectopic Gland from Adult Patient [Pubmed:29593791] |
Archives?Animal?Breeding | Age-related changes in testicular parameters and their relationship to thyroid hormones and testosterone in male Murrah buffaloes [10.5194:aab-61-191-2018] |
Environmental Pollution | Transgenerational thyroid endocrine disruption induced by bisphenol S affects the early development of zebrafish offspring [Pubmed: 30243188] |
Journal of Agricultural and?Food?Chemistry | Metabolism of diflubenzuron in lizard (Eremias argus) and comparative toxicity of diflubenzuron and its metabolite [Pubmed: 30346759] |
Journal of Veterinary?Research | Elastographic and morphological testicular changes in hypothyroidism–an experimental study [Doi: 10.2478/jvetres-2018-0049] |
Science of The?Total?Environment | Dose-dependent effects of flufenoxuron on thyroid system of mature female lizards (Eremias argus) and their offspring [Pubmed: 30448662] |
Cell?Reports | Zika Virus Infection in Hypothalamus Causes Hormone Deficiencies and Leads to Irreversible Growth Delay and Memory Impairment in Mice [Pubmed: 30404008] |
Drug Metabolism and Disposition | Serotonin receptors of 5-HT2 type in the hypothalamic arcuate nuclei (ARC) positively regulate liver cytochrome P450 via stimulation of the growth hormone-releasing?… [Pubmed: 30518657] |
Science?of?the?Total?Environment | Parental exposure to 6: 2 chlorinated polyfluorinated ether sulfonate (F53B) induced transgenerational thyroid hormone disruption in zebrafish [Doi: 10.1016/j.scitotenv.2019.02.198] |
Chemosphere | Inhibition of growth in juvenile manila clam Ruditapes philippinarum: Potential adverse outcome pathway of TBBPA [Pubmed: 30844590] |
Journal of Veterinary?Research | Pituitary-testicular axis dysfunction in methimazole-induced hypothyroidism in rats [Doi: 10.2478/jvetres-2019-0008] |
Journal of Diabetes Research | The Adverse Effects of Thyrotropin Absence on Pancreatic β Cell Function in Mice [] |
Aquatic Toxicology | Effects of cis-bifenthrin enantiomers on the growth, behavioral, biomarkers of oxidative damage and bioaccumulation in Xenopus laevis [Pubmed: 31276910] |
ecotoxicology and environmental safety | Exposure to humidifier disinfectants induces developmental effects and disrupts thyroid endocrine systems in zebrafish larvae [Pubmed: 31539807] |
Chemosphere | Exposure of adult mice to perfluorobutanesulfonate impacts ovarian functions through hypothyroxinemia leading to down-regulation of Akt-mTOR signaling [Pubmed: 31809938] |
Autoimmunity | Therapeutic effect of mesenchymal stem cell on Hashimoto's thyroiditis in a rat model by modulating Th17/Treg cell balance [Pubmed: 31793369] |
AQUATIC TOXICOLOGY | Interaction between hypoxia and perfluorobutane sulfonate on developmental toxicity and endocrine disruption in marine medaka embryos [Pubmed: 32172180] |
Clinical Immunology | The role of protein disulphide-isomerase A3 as autoantigen in the pathogenesis of autoimmune thyroiditis and related brain damage in adult mice [Pubmed: 31982645] |
Chemosphere | Effect of triadimefon and its metabolite on adult amphibians Xenopus laevis [Pubmed: 31743868] |
Nature | Feeding induces cholesterol biosynthesis via the mTORC1–USP20–HMGCR axis [Pubmed: 33177714] |
Toxicol Lett | Sex-specific effects of triphenyltin chloride (TPT) on thyroid disruption and metabolizing enzymes in adult zebrafish (Danio rerio) [Pubmed: 32525014] |
Ecotoxicology | Changes in thyroid hormone levels and related gene expressions in embryo–larval zebraish exposed to binary combinations of bifenthrin and acetochlor [Pubmed: 32468518] |
Int Immunopharmacol | Experimental evidence for alpha enolase as one potential autoantigen in the pathogenesis of both autoimmune thyroiditis and its related encephalopathy [Pubmed: 32442899] |
BioMed Research International | Pathophysiological Changes in Female Rats with Estrous Cycle Disorder Induced by Long-Term Heat Stress [Pubmed: 32685488] |
Brazilian Journal of Development | Maternal exposure of triclosan causes fetal development restriction and female reproductive alterations in rat offspring/A exposi??o materna ao triclosan causa?… [] |
CHEMOSPHERE | Effects of 2-ethylhexyl-4-methoxycinnamate (EHMC) on thyroid hormones and genes associated with thyroid, neurotoxic, and nephrotoxic responses in adult and larval zebrafish (Danio rerio) [] |
Andrologia | Thyroid hormones, Sertoli cell proliferation and differentiation in progenies from carbamazepine?\treated rat dams during pregnancy and lactation [33433934] |
The Journal of Clinical Endocrinology & Metabolism | High levels of thyroid hormone impair regulatory T cells function via reduced PD-1 expression [33758937] |
Endocrinology | Genetic Manipulation on Zebrafish duox Recapitulate the Clinical Manifestations of Congenital Hypothyroidism [34019632] |
Ecotoxicol Environ Saf | Xenopus laevis tadpoles exposed to metamifop: Changes in growth, behavioral endpoints, neurotransmitters, antioxidant system and thyroid development [34126306] |
Clin Epigenetics | Genome?wide profiling of DNA methylation and gene expression unravel the epigenetic landscape in diabetes-related hypothyroidism [34092241] |
Journal of Hazardous Materials | Exposure of adult zebrafish (Danio rerio) to Tetrabromobisphenol A causes neurotoxicity in larval offspring, an adverse transgenerational effect [33647619] |
Pharmaceutics | The Selective NMDA Receptor GluN2B Subunit Antagonist CP-101,606 with Antidepressant Properties Modulates Cytochrome P450 Expression in the Liver [34683936] |
Aquat Toxicol | Thyroid disruption and growth inhibition of zebrafish embryos/larvae by phenanthrene treatment at environmentally relevant concentrations [34933138] |
Ecotoxicol Environ Saf | Perfluorohexanoic acid caused disruption of the hypothalamus-pituitary-thyroid axis in zebrafish larvae [Pubmed:35131581] |
Toxics | Environmental Co-Exposure to Potassium Perchlorate and Cd Caused Toxicity and Thyroid Endocrine Disruption in Zebrafish Embryos and Larvae (Danio rerio) [Pubmed:35448459] |
Fluorene-9-Bisphenol Induced Thyroid-Disrupting Effects and Histopathological Alterations in Adult Zebrafish (Danio Rerio) [] |
留言咨詢