甲狀腺素(T4)檢測試劑盒(酶聯免疫吸附試驗法,快捷型)
Instant ELISA Kit for Thyroxine (T4)
3,5,3',5'-Tetraiodothyronine; L-Thyroxine; Levothyroxine
-
產品包裝(模擬)
-
產品包裝(模擬)
-
實驗結果圖
-
通過ISO 9001、ISO 13485質量體系認證
特異性
本試劑盒用于檢測甲狀腺素(T4)檢測試劑盒(酶聯免疫吸附試驗法,快捷型)，經檢測與其它相似物質無明顯交叉反應。
由于受到技術及樣本來源的限制，不可能完成對所有相關或相似物質交叉反應檢測，因此本試劑盒有可能與未經檢測的其它物質有交叉反應。
回收率
分別于定值血清及血漿樣本中加入一定量的甲狀腺素(T4)檢測試劑盒(酶聯免疫吸附試驗法,快捷型)（加標樣品），重復測定并計算其均值，回收率為測定值與理論值的比率。
| 樣本 | 回收率范圍(%) | 平均回收率(%) |
| serum(n=5) | 78-98 | 90 |
| EDTA plasma(n=5) | 78-104 | 84 |
| heparin plasma(n=5) | 88-97 | 93 |
精密度
精密度用樣品測定值的變異系數CV表示。CV(%) = SD/mean×100
批內差：取同批次試劑盒對低、中、高值定值樣本進行定量檢測,每份樣本連續(xù)測定20 次，分別計算不同濃度樣本的平均值及SD值。
批間差：選取3個不同批次的試劑盒分別對低、中、高值定值樣本進行定量測定，每個樣本使用同一試劑盒重復測定8次，分別計算不同濃度樣本的平均值及SD值。
批內差： CV<10%
批間差： CV<12%
線性
在定值血清及血漿樣本內加入適量的甲狀腺素(T4)檢測試劑盒(酶聯免疫吸附試驗法,快捷型)，并倍比稀釋成1:2，1:4，1:8，1:16的待測樣本，線性范圍即為稀釋后樣本中甲狀腺素(T4)檢測試劑盒(酶聯免疫吸附試驗法,快捷型)含量的測定值與理論值的比率。
| 樣本 | 1:2 | 1:4 | 1:8 | 1:16 |
| serum(n=5) | 92-99% | 85-93% | 78-99% | 80-103% |
| EDTA plasma(n=5) | 78-90% | 83-94% | 89-102% | 81-96% |
| heparin plasma(n=5) | 80-101% | 98-105% | 88-104% | 79-96% |
穩(wěn)定性
經測定，試劑盒在有效期內按推薦溫度保存，其活性降低率小于5%。
為減小外部因素對試劑盒破壞前后檢測值的影響，實驗室的環(huán)境條件需盡量保持一致，尤其是實驗室內溫度、濕度及溫育條件。其次由同一實驗員來進行操作可減少人為誤差。
實驗流程
1. 實驗前標準品、試劑及樣本的準備；
2. 加樣（標準品及樣本）100µL，37°C溫育30分鐘;
3. 甩干，加檢測溶液A100µL，37°C溫育30分鐘;
4. 洗板3次；
5. 加檢測溶液B100µL， 37°C孵育10分鐘；
6. 洗板5次；
7. 加TMB底物90µL，37°C孵育10－20分鐘；
8. 加終止液50µL，立即450nm讀數。
實驗原理
將甲狀腺素(T4)檢測試劑盒(酶聯免疫吸附試驗法,快捷型)單克隆抗體包被于96孔微孔板中，制成固相載體，向微孔中加入標準品或標本以及同時加入HRP標記甲狀腺素(T4)檢測試劑盒(酶聯免疫吸附試驗法,快捷型)的多克隆抗體，孵育結束徹底洗滌后加入TMB底物顯色。TMB在過氧化物酶的催化下轉化成藍色，并在酸的作用下轉化成最終的黃色。顏色的深淺和樣品中的被檢測物濃度呈正相關。用酶標儀在450nm波長下測定吸光度(O.D.值)，計算樣品濃度。
相關產品
| 編號 | 適用物種：Pan-species (General，通用) | 應用(僅供研究使用，不用于臨床診斷！) |
| CPA452Ge11 | 甲狀腺素(T4)牛血清白蛋白偶聯物 | Immunogen; SDS-PAGE; WB. |
| CPA452Ge21 | 甲狀腺素(T4)卵白蛋白偶聯物 | Immunogen; SDS-PAGE; WB. |
| PAA452Ge01 | 甲狀腺素(T4)多克隆抗體 | ELISA, CLIA. / IHC-Fr, ICC, IP (predicted). |
| LAA452Ge81 | 甲狀腺素(T4)多克隆抗體(異硫氰酸熒光素標記) | WB; IHC; ICC; IF. |
| MAA452Ge28 | 甲狀腺素(T4)單克隆抗體 | ELISA, CLIA. / IHC-Fr, ICC, IP (predicted). |
| MAA452Ge25 | 甲狀腺素(T4)單克隆抗體 | ELISA, CLIA. / IHC-Fr, ICC, IP (predicted). |
| MAA452Ge23 | 甲狀腺素(T4)單克隆抗體 | ELISA, CLIA. / IHC-Fr, ICC, IP (predicted). |
| MAA452Ge22 | 甲狀腺素(T4)單克隆抗體 | ELISA, CLIA. / IHC-Fr, ICC, IP (predicted). |
| MAA452Ge21 | 甲狀腺素(T4)單克隆抗體 | ELISA, CLIA. / IHC-Fr, ICC, IP (predicted). |
| CEA452Ge | 甲狀腺素(T4)檢測試劑盒(酶聯免疫吸附試驗法) | Enzyme-linked immunosorbent assay for Antigen Detection. |
| IEA452Ge | 甲狀腺素(T4)檢測試劑盒(酶聯免疫吸附試驗法,快捷型) | Enzyme-linked immunosorbent assay for Antigen Detection. |
| MEA452Ge | 甲狀腺素(T4)檢測試劑盒(酶聯免疫吸附試驗法,小樣本) | Enzyme-linked immunosorbent assay for Antigen Detection. |
| CCA452Ge | 甲狀腺素(T4)檢測試劑盒(化學發(fā)光免疫分析法) | Chemiluminescent immunoassay for Antigen Detection. |
| LMA452Ge | 甲狀腺素(T4)等多因子檢測試劑盒(流式熒光發(fā)光法) | FLIA Kit for Antigen Detection. |
| KSA452Ge11 | 甲狀腺素(T4)檢測試劑盒DIY材料(酶聯免疫吸附試驗法) | 自己動手制作ELISA試劑盒的主要材料 |
參考文獻
| 雜志 | 參考文獻 |
| Aquatic Toxicology | Bioconcentration and metabolism of decabromodiphenyl ether (BDE-209) result in thyroid endocrine disruption in zebrafish larvae [ScienceDirect: S0166445X12000215] |
| Aquatic Toxicology | Exposure of zebrafish embryos/larvae to TDCPP alters concentrations of thyroid hormones and transcriptions of genes involved in the hypothalamic–pituitary–thyroid axis [PubMed: 23220413] |
| Life Science Journal | Can Nigella Sativa oil (NSO) reverse hypothyroid status induced by PTU in rat? biochemical and histological studies [Lifesciencesite: Source] |
| Aquat Toxicol | Thyroid endocrine system disruption by pentachlorophenol: an in vitro and in vivo assay. [ScienceDirect: S0166445X13002129] |
| Aquatic Toxicology | Impact of co-exposure with lead and decabromodiphenyl ether (BDE-209) on thyroid function in zebrafish larvae [Pubmed:25456233] |
| Chemosphere | Exposure to2,2′,4,4′-tetrabromodiphenyl ether (BDE-47) alters thyroid hormone levels and thyroid hormone-reCavia (Guinea pig )lated gene transcription in manila clam?Ruditapes philippinarum [science:S0045653516302016] |
| Frontiers in cellular neuroscience | Elevated Thyroid Peroxidase Antibody Increases Risk of Post-partum Depression by Decreasing Prefrontal Cortex BDNF and 5-HT Levels in Mice [pubmed:28119573] |
| Toxicological?Sciences | Exposure of pregnant mice to perfluorobutanesulfonate causes hypothyroxinemia and developmental abnormalities in female offspring [pubmed:27803384] |
| Sci Rep | Exposure of pregnant mice to triclosan impairs placental development and nutrient transport. [pubmed:28322267] |
| Aquat Toxicol. | Developmental toxicity and thyroid hormone-disrupting effects of 2,4-dichloro-6-nitrophenol in Chinese rare minnow (Gobiocypris rarus). [pubmed:28187359] |
| Bulletin of the World Health Organization | Endemic goitre in the Sudan despite longstanding programmes for the control of iodine deficiency disorders [handle:123456789] |
| Biological Rhythm Research | Age-related and seasonal variations in plasma uncarboxylated osteocalcin in male Murrah buffaloes [09291016.2016.1275393] |
| Toxicological Sciences | Exposure of Pregnant Mice to Triclosan Causes Insulin Resistance via Thyroxine Reduction [pubmed:28973666] |
| Environmental Pollution | Transgenerational endocrine disruption and neurotoxicity in zebrafish larvae after parental exposure to binary mixtures of decabromodiphenyl ether (BDE-209) and lead [10.1016/j.envpol.2017.06.053] |
| Sciencepaper online | 四溴雙酚A對菲律賓蛤仔的生長抑制效應機理Growth inhibition Effects of TBBPA on juvenile manila clam Ruditapes philippinarum [5790267088906950000] |
| Tropical Journal of Pharmaceutical Research | Neuroprotective effects of thymoquinone against cerebellar histopathological changes in propylthiouracil-induced hypothyroidism in adult rats [10.4314/tjpr.v16i5.9] |
| s-space | Thyroid disrupting effects and associated mechanisms of TCPP in GH3 cell line and zebrafish (Danio rerio) larva and adult [10371/137708] |
| Environmental Pollution | Time-dependent inhibitory effects of Tris(1, 3-dichloro-2-propyl) phosphate on growth and transcription of genes involved in the GH/IGF axis, but not the HPT axis, in female zebrafish. [pubmed:28624628] |
| Environmental pollution | Stereoselective degradation and thyroid endocrine disruption of lambda-cyhalothrin in lizards (Eremias argus) following oral exposure [pubmed:28970022] |
| Environmental?Science?& Technology | Thyroid hormone-disrupting potentials of major benzophenones in two cell lines (GH3 and FRTL-5) and embryo-larval zebrafish [Pubmed:29995391] |
| Environmental Pollution | Prenatal exposure to polychlorinated biphenyl and umbilical cord hormones and birth outcomes in an island population [Pubmed:29525625] |
| Toxicological?Sciences | Impact of Perfluorooctane Sulfonate on Reproductive Ability of Female Mice through Suppression of Estrogen Receptor a-Activated Kisspeptin Neurons [10.1093:toxsci] |
| Frontiers in Molecular Neuroscience | Impact of Triclosan on Female Reproduction through Reducing Thyroid Hormones to Suppress Hypothalamic Kisspeptin Neurons in Mice [Pubmed:29403355] |
| Drug?metabolism and disposition | Activation of 5-HT1A receptors in the hypothalamic paraventricular nuclei (PVN) negatively regulates cytochrome P450 expression and activity in rat liver [Pubmed:29555828] |
| International Journal of Endocrinology | Histopathological Characterization and Whole Exome Sequencing of Ectopic Thyroid: Fetal Architecture in a Functional Ectopic Gland from Adult Patient [Pubmed:29593791] |
| Archives?Animal?Breeding | Age-related changes in testicular parameters and their relationship to thyroid hormones and testosterone in male Murrah buffaloes [10.5194:aab-61-191-2018] |
| Environmental Pollution | Transgenerational thyroid endocrine disruption induced by bisphenol S affects the early development of zebrafish offspring [Pubmed: 30243188] |
| Journal of Agricultural and?Food?Chemistry | Metabolism of diflubenzuron in lizard (Eremias argus) and comparative toxicity of diflubenzuron and its metabolite [Pubmed: 30346759] |
| Journal of Veterinary?Research | Elastographic and morphological testicular changes in hypothyroidism–an experimental study [Doi: 10.2478/jvetres-2018-0049] |
| Science of The?Total?Environment | Dose-dependent effects of flufenoxuron on thyroid system of mature female lizards (Eremias argus) and their offspring [Pubmed: 30448662] |
| Cell?Reports | Zika Virus Infection in Hypothalamus Causes Hormone Deficiencies and Leads to Irreversible Growth Delay and Memory Impairment in Mice [Pubmed: 30404008] |
| Drug Metabolism and Disposition | Serotonin receptors of 5-HT2 type in the hypothalamic arcuate nuclei (ARC) positively regulate liver cytochrome P450 via stimulation of the growth hormone-releasing?… [Pubmed: 30518657] |
| Science?of?the?Total?Environment | Parental exposure to 6: 2 chlorinated polyfluorinated ether sulfonate (F53B) induced transgenerational thyroid hormone disruption in zebrafish [Doi: 10.1016/j.scitotenv.2019.02.198] |
| Chemosphere | Inhibition of growth in juvenile manila clam Ruditapes philippinarum: Potential adverse outcome pathway of TBBPA [Pubmed: 30844590] |
| Journal of Veterinary?Research | Pituitary-testicular axis dysfunction in methimazole-induced hypothyroidism in rats [Doi: 10.2478/jvetres-2019-0008] |
| Journal of Diabetes Research | The Adverse Effects of Thyrotropin Absence on Pancreatic β Cell Function in Mice [] |
| Aquatic Toxicology | Effects of cis-bifenthrin enantiomers on the growth, behavioral, biomarkers of oxidative damage and bioaccumulation in Xenopus laevis [Pubmed: 31276910] |
| ecotoxicology and environmental safety | Exposure to humidifier disinfectants induces developmental effects and disrupts thyroid endocrine systems in zebrafish larvae [Pubmed: 31539807] |
| Chemosphere | Exposure of adult mice to perfluorobutanesulfonate impacts ovarian functions through hypothyroxinemia leading to down-regulation of Akt-mTOR signaling [Pubmed: 31809938] |
| Autoimmunity | Therapeutic effect of mesenchymal stem cell on Hashimoto's thyroiditis in a rat model by modulating Th17/Treg cell balance [Pubmed: 31793369] |
| AQUATIC TOXICOLOGY | Interaction between hypoxia and perfluorobutane sulfonate on developmental toxicity and endocrine disruption in marine medaka embryos [Pubmed: 32172180] |
| Clinical Immunology | The role of protein disulphide-isomerase A3 as autoantigen in the pathogenesis of autoimmune thyroiditis and related brain damage in adult mice [Pubmed: 31982645] |
| Chemosphere | Effect of triadimefon and its metabolite on adult amphibians Xenopus laevis [Pubmed: 31743868] |
| Nature | Feeding induces cholesterol biosynthesis via the mTORC1–USP20–HMGCR axis [Pubmed: 33177714] |
| Toxicol Lett | Sex-specific effects of triphenyltin chloride (TPT) on thyroid disruption and metabolizing enzymes in adult zebrafish (Danio rerio) [Pubmed: 32525014] |
| Ecotoxicology | Changes in thyroid hormone levels and related gene expressions in embryo–larval zebraish exposed to binary combinations of bifenthrin and acetochlor [Pubmed: 32468518] |
| Int Immunopharmacol | Experimental evidence for alpha enolase as one potential autoantigen in the pathogenesis of both autoimmune thyroiditis and its related encephalopathy [Pubmed: 32442899] |
| BioMed Research International | Pathophysiological Changes in Female Rats with Estrous Cycle Disorder Induced by Long-Term Heat Stress [Pubmed: 32685488] |
| Brazilian Journal of Development | Maternal exposure of triclosan causes fetal development restriction and female reproductive alterations in rat offspring/A exposi??o materna ao triclosan causa?… [] |
| CHEMOSPHERE | Effects of 2-ethylhexyl-4-methoxycinnamate (EHMC) on thyroid hormones and genes associated with thyroid, neurotoxic, and nephrotoxic responses in adult and larval zebrafish (Danio rerio) [] |
| Andrologia | Thyroid hormones, Sertoli cell proliferation and differentiation in progenies from carbamazepine?\treated rat dams during pregnancy and lactation [33433934] |
| The Journal of Clinical Endocrinology & Metabolism | High levels of thyroid hormone impair regulatory T cells function via reduced PD-1 expression [33758937] |
| Endocrinology | Genetic Manipulation on Zebrafish duox Recapitulate the Clinical Manifestations of Congenital Hypothyroidism [34019632] |
| Ecotoxicol Environ Saf | Xenopus laevis tadpoles exposed to metamifop: Changes in growth, behavioral endpoints, neurotransmitters, antioxidant system and thyroid development [34126306] |
| Clin Epigenetics | Genome?wide profiling of DNA methylation and gene expression unravel the epigenetic landscape in diabetes-related hypothyroidism [34092241] |
| Journal of Hazardous Materials | Exposure of adult zebrafish (Danio rerio) to Tetrabromobisphenol A causes neurotoxicity in larval offspring, an adverse transgenerational effect [33647619] |
| Pharmaceutics | The Selective NMDA Receptor GluN2B Subunit Antagonist CP-101,606 with Antidepressant Properties Modulates Cytochrome P450 Expression in the Liver [34683936] |
| Aquat Toxicol | Thyroid disruption and growth inhibition of zebrafish embryos/larvae by phenanthrene treatment at environmentally relevant concentrations [34933138] |
| Ecotoxicol Environ Saf | Perfluorohexanoic acid caused disruption of the hypothalamus-pituitary-thyroid axis in zebrafish larvae [Pubmed:35131581] |
| Toxics | Environmental Co-Exposure to Potassium Perchlorate and Cd Caused Toxicity and Thyroid Endocrine Disruption in Zebrafish Embryos and Larvae (Danio rerio) [Pubmed:35448459] |
| Fluorene-9-Bisphenol Induced Thyroid-Disrupting Effects and Histopathological Alterations in Adult Zebrafish (Danio Rerio) [] |
留言咨詢
