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脂多糖結(jié)合蛋白(LBP)檢測試劑盒(酶聯(lián)免疫吸附試驗法,高敏型)

High Sensitive ELISA Kit for Lipopolysaccharide Binding Protein (LBP)

LPS-Binding Protein

  • 脂多糖結(jié)合蛋白(LBP)檢測試劑盒(酶聯(lián)免疫吸附試驗法,高敏型) 產(chǎn)品包裝(模擬)
  • 脂多糖結(jié)合蛋白(LBP)檢測試劑盒(酶聯(lián)免疫吸附試驗法,高敏型) 產(chǎn)品包裝(模擬)
  • 脂多糖結(jié)合蛋白(LBP)檢測試劑盒(酶聯(lián)免疫吸附試驗法,高敏型) 實驗結(jié)果圖
  • HEB406Ra.jpg 標(biāo)準(zhǔn)曲線圖
  • Certificate 通過ISO 9001、ISO 13485質(zhì)量體系認證

特異性

本試劑盒用于檢測脂多糖結(jié)合蛋白(LBP)檢測試劑盒(酶聯(lián)免疫吸附試驗法,高敏型),經(jīng)檢測與其它相似物質(zhì)無明顯交叉反應(yīng)。
由于受到技術(shù)及樣本來源的限制,不可能完成對所有相關(guān)或相似物質(zhì)交叉反應(yīng)檢測,因此本試劑盒有可能與未經(jīng)檢測的其它物質(zhì)有交叉反應(yīng)。

回收率

分別于定值血清及血漿樣本中加入一定量的脂多糖結(jié)合蛋白(LBP)檢測試劑盒(酶聯(lián)免疫吸附試驗法,高敏型)(加標(biāo)樣品),重復(fù)測定并計算其均值,回收率為測定值與理論值的比率。

樣本 回收率范圍(%) 平均回收率(%)
serum(n=5) 81-91 86
EDTA plasma(n=5) 88-96 93
heparin plasma(n=5) 90-97 94

精密度

精密度用樣品測定值的變異系數(shù)CV表示。CV(%) = SD/mean×100
批內(nèi)差:取同批次試劑盒對低、中、高值定值樣本進行定量檢測,每份樣本連續(xù)測定20 次,分別計算不同濃度樣本的平均值及SD值。
批間差:選取3個不同批次的試劑盒分別對低、中、高值定值樣本進行定量測定,每個樣本使用同一試劑盒重復(fù)測定8次,分別計算不同濃度樣本的平均值及SD值。
批內(nèi)差: CV<10%
批間差: CV<12%

線性

在定值血清及血漿樣本內(nèi)加入適量的脂多糖結(jié)合蛋白(LBP)檢測試劑盒(酶聯(lián)免疫吸附試驗法,高敏型),并倍比稀釋成1:2,1:4,1:8,1:16的待測樣本,線性范圍即為稀釋后樣本中脂多糖結(jié)合蛋白(LBP)檢測試劑盒(酶聯(lián)免疫吸附試驗法,高敏型)含量的測定值與理論值的比率。

樣本 1:2 1:4 1:8 1:16
serum(n=5) 83-91% 88-101% 81-102% 94-102%
EDTA plasma(n=5) 83-95% 78-90% 94-102% 79-89%
heparin plasma(n=5) 85-102% 97-104% 94-102% 99-105%

穩(wěn)定性

經(jīng)測定,試劑盒在有效期內(nèi)按推薦溫度保存,其活性降低率小于5%。
為減小外部因素對試劑盒破壞前后檢測值的影響,實驗室的環(huán)境條件需盡量保持一致,尤其是實驗室內(nèi)溫度、濕度及溫育條件。其次由同一實驗員來進行操作可減少人為誤差。

實驗流程

1. 實驗前標(biāo)準(zhǔn)品、試劑及樣本的準(zhǔn)備;
2. 加樣(標(biāo)準(zhǔn)品及樣本)100µL,37°C孵育1小時;
3. 吸棄,加檢測溶液A100µL,37°C孵育1小時;
4. 洗板3次;
5. 加檢測溶液B100µL,37°C孵育30分鐘;
6. 洗板5次;
7. 加TMB底物90µL,37°C孵育10-20分鐘;
8. 加終止液50µL,立即450nm讀數(shù)。

實驗原理

將脂多糖結(jié)合蛋白(LBP)檢測試劑盒(酶聯(lián)免疫吸附試驗法,高敏型)抗體包被于96孔微孔板中,制成固相載體,向微孔中分別加入標(biāo)準(zhǔn)品或標(biāo)本,其中的脂多糖結(jié)合蛋白(LBP)檢測試劑盒(酶聯(lián)免疫吸附試驗法,高敏型)與連接于固相載體上的抗體結(jié)合,然后加入生物素化的脂多糖結(jié)合蛋白(LBP)檢測試劑盒(酶聯(lián)免疫吸附試驗法,高敏型)抗體,將未結(jié)合的生物素化抗體洗凈后,加入HRP標(biāo)記的親和素,再次徹底洗滌后加入TMB底物顯色。TMB在過氧化物酶的催化下轉(zhuǎn)化成藍色,并在酸的作用下轉(zhuǎn)化成最終的黃色。顏色的深淺和樣品中的脂多糖結(jié)合蛋白(LBP)檢測試劑盒(酶聯(lián)免疫吸附試驗法,高敏型)呈正相關(guān)。用酶標(biāo)儀在450nm波長下測定吸光度(O.D.值),計算樣品濃度。

相關(guān)產(chǎn)品

編號 適用物種:Rattus norvegicus (Rat,大鼠) 應(yīng)用(僅供研究使用,不用于臨床診斷!)
RPB406Ra02 脂多糖結(jié)合蛋白(LBP)重組蛋白 Positive Control; Immunogen; SDS-PAGE; WB.
RPB406Ra01 脂多糖結(jié)合蛋白(LBP)重組蛋白 Positive Control; Immunogen; SDS-PAGE; WB.
PAB406Ra01 脂多糖結(jié)合蛋白(LBP)多克隆抗體 WB; IHC; ICC; IP.
PAB406Ra02 脂多糖結(jié)合蛋白(LBP)多克隆抗體 WB; IHC; ICC; IP.
LAB406Ra41 脂多糖結(jié)合蛋白(LBP)多克隆抗體(藻紅素標(biāo)記) WB; IHC; ICC; IF.
MAB406Ra21 脂多糖結(jié)合蛋白(LBP)單克隆抗體 WB; IHC; ICC; IP.
SEB406Ra 脂多糖結(jié)合蛋白(LBP)檢測試劑盒(酶聯(lián)免疫吸附試驗法) Enzyme-linked immunosorbent assay for Antigen Detection.
HEB406Ra 脂多糖結(jié)合蛋白(LBP)檢測試劑盒(酶聯(lián)免疫吸附試驗法,高敏型) Enzyme-linked immunosorbent assay for Antigen Detection.
LMB406Ra 脂多糖結(jié)合蛋白(LBP)等多因子檢測試劑盒(流式熒光發(fā)光法) FLIA Kit for Antigen Detection.

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