脂多糖結(jié)合蛋白(LBP)檢測試劑盒(酶聯(lián)免疫吸附試驗法,高敏型)
High Sensitive ELISA Kit for Lipopolysaccharide Binding Protein (LBP)
LPS-Binding Protein
- 編號HEB406Ra
- 物種Rattus norvegicus (Rat,大鼠) 相同的名稱,不同的物種。
- 實驗方法雙抗夾心
- 反應(yīng)時長3h
- 檢測范圍15.6-1,000pg/mL
- 靈敏度最小可檢測劑量小于等于6.0pg/mL.
- 樣本類型Serum, plasma and other biological fluids
- 下載 英文說明書 中文說明書
- 規(guī)格 48T96T 96T*5 96T*10 96T*100
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特異性
本試劑盒用于檢測脂多糖結(jié)合蛋白(LBP)檢測試劑盒(酶聯(lián)免疫吸附試驗法,高敏型),經(jīng)檢測與其它相似物質(zhì)無明顯交叉反應(yīng)。
由于受到技術(shù)及樣本來源的限制,不可能完成對所有相關(guān)或相似物質(zhì)交叉反應(yīng)檢測,因此本試劑盒有可能與未經(jīng)檢測的其它物質(zhì)有交叉反應(yīng)。
回收率
分別于定值血清及血漿樣本中加入一定量的脂多糖結(jié)合蛋白(LBP)檢測試劑盒(酶聯(lián)免疫吸附試驗法,高敏型)(加標(biāo)樣品),重復(fù)測定并計算其均值,回收率為測定值與理論值的比率。
樣本 | 回收率范圍(%) | 平均回收率(%) |
serum(n=5) | 81-91 | 86 |
EDTA plasma(n=5) | 88-96 | 93 |
heparin plasma(n=5) | 90-97 | 94 |
精密度
精密度用樣品測定值的變異系數(shù)CV表示。CV(%) = SD/mean×100
批內(nèi)差:取同批次試劑盒對低、中、高值定值樣本進行定量檢測,每份樣本連續(xù)測定20 次,分別計算不同濃度樣本的平均值及SD值。
批間差:選取3個不同批次的試劑盒分別對低、中、高值定值樣本進行定量測定,每個樣本使用同一試劑盒重復(fù)測定8次,分別計算不同濃度樣本的平均值及SD值。
批內(nèi)差: CV<10%
批間差: CV<12%
線性
在定值血清及血漿樣本內(nèi)加入適量的脂多糖結(jié)合蛋白(LBP)檢測試劑盒(酶聯(lián)免疫吸附試驗法,高敏型),并倍比稀釋成1:2,1:4,1:8,1:16的待測樣本,線性范圍即為稀釋后樣本中脂多糖結(jié)合蛋白(LBP)檢測試劑盒(酶聯(lián)免疫吸附試驗法,高敏型)含量的測定值與理論值的比率。
樣本 | 1:2 | 1:4 | 1:8 | 1:16 |
serum(n=5) | 83-91% | 88-101% | 81-102% | 94-102% |
EDTA plasma(n=5) | 83-95% | 78-90% | 94-102% | 79-89% |
heparin plasma(n=5) | 85-102% | 97-104% | 94-102% | 99-105% |
穩(wěn)定性
經(jīng)測定,試劑盒在有效期內(nèi)按推薦溫度保存,其活性降低率小于5%。
為減小外部因素對試劑盒破壞前后檢測值的影響,實驗室的環(huán)境條件需盡量保持一致,尤其是實驗室內(nèi)溫度、濕度及溫育條件。其次由同一實驗員來進行操作可減少人為誤差。
實驗流程
1. 實驗前標(biāo)準(zhǔn)品、試劑及樣本的準(zhǔn)備;
2. 加樣(標(biāo)準(zhǔn)品及樣本)100µL,37°C孵育1小時;
3. 吸棄,加檢測溶液A100µL,37°C孵育1小時;
4. 洗板3次;
5. 加檢測溶液B100µL,37°C孵育30分鐘;
6. 洗板5次;
7. 加TMB底物90µL,37°C孵育10-20分鐘;
8. 加終止液50µL,立即450nm讀數(shù)。
實驗原理
將脂多糖結(jié)合蛋白(LBP)檢測試劑盒(酶聯(lián)免疫吸附試驗法,高敏型)抗體包被于96孔微孔板中,制成固相載體,向微孔中分別加入標(biāo)準(zhǔn)品或標(biāo)本,其中的脂多糖結(jié)合蛋白(LBP)檢測試劑盒(酶聯(lián)免疫吸附試驗法,高敏型)與連接于固相載體上的抗體結(jié)合,然后加入生物素化的脂多糖結(jié)合蛋白(LBP)檢測試劑盒(酶聯(lián)免疫吸附試驗法,高敏型)抗體,將未結(jié)合的生物素化抗體洗凈后,加入HRP標(biāo)記的親和素,再次徹底洗滌后加入TMB底物顯色。TMB在過氧化物酶的催化下轉(zhuǎn)化成藍色,并在酸的作用下轉(zhuǎn)化成最終的黃色。顏色的深淺和樣品中的脂多糖結(jié)合蛋白(LBP)檢測試劑盒(酶聯(lián)免疫吸附試驗法,高敏型)呈正相關(guān)。用酶標(biāo)儀在450nm波長下測定吸光度(O.D.值),計算樣品濃度。
增值服務(wù)
相關(guān)產(chǎn)品
編號 | 適用物種:Rattus norvegicus (Rat,大鼠) | 應(yīng)用(僅供研究使用,不用于臨床診斷!) |
RPB406Ra02 | 脂多糖結(jié)合蛋白(LBP)重組蛋白 | Positive Control; Immunogen; SDS-PAGE; WB. |
RPB406Ra01 | 脂多糖結(jié)合蛋白(LBP)重組蛋白 | Positive Control; Immunogen; SDS-PAGE; WB. |
PAB406Ra01 | 脂多糖結(jié)合蛋白(LBP)多克隆抗體 | WB; IHC; ICC; IP. |
PAB406Ra02 | 脂多糖結(jié)合蛋白(LBP)多克隆抗體 | WB; IHC; ICC; IP. |
LAB406Ra41 | 脂多糖結(jié)合蛋白(LBP)多克隆抗體(藻紅素標(biāo)記) | WB; IHC; ICC; IF. |
MAB406Ra21 | 脂多糖結(jié)合蛋白(LBP)單克隆抗體 | WB; IHC; ICC; IP. |
SEB406Ra | 脂多糖結(jié)合蛋白(LBP)檢測試劑盒(酶聯(lián)免疫吸附試驗法) | Enzyme-linked immunosorbent assay for Antigen Detection. |
HEB406Ra | 脂多糖結(jié)合蛋白(LBP)檢測試劑盒(酶聯(lián)免疫吸附試驗法,高敏型) | Enzyme-linked immunosorbent assay for Antigen Detection. |
LMB406Ra | 脂多糖結(jié)合蛋白(LBP)等多因子檢測試劑盒(流式熒光發(fā)光法) | FLIA Kit for Antigen Detection. |
參考文獻
雜志 | 參考文獻 |
37 | A Marker of Endotoxemia Is Associated With Obesity and Related Metabolic Disorders in Apparently Healthy Chinese [Diabetes: source] |
AIDS | Oral serum-derived bovine immunoglobulin improves duodenal immune reconstitution and absorption function in patients with HIV enteropathy [PubMed: PMC3754419] |
31 | Gut microbiome composition is linked to whole grain-induced immunological improvements [Pubmed: 23038174] |
The Veterinary Journal | High ambient temperature alleviates the inflammatory response and growth depression in pigs challenged with Escherichia coli lipopolysaccharide [Pubmed: 24792207] |
Perfusion | The effect of normovolemic modified ultrafiltration on inflammatory mediators, endotoxins, terminal complement complexes and clinical outcome in high-risk cardiac surgery patients [Pubmed: 23429100] |
FEMS Microbiology Ecology | A gut microbiota-targeted dietary intervention for amelioration of chronic inflammation underlying metabolic syndrome. [Pubmed: 24117923] |
Digestive diseases and sciences | Frequency and Severity of Cirrhotic Cardiomyopathy and Its Possible Relationship with Bacterial Endotoxemia [Pubmed: 23907333] |
Atherosclerosis | Circulating pathogen associated molecular pattern – Binding proteins and High Mobility Group Box protein 1 in nascent metabolic syndrome: Implications for cellular Toll-like receptor activity [Pubmed:25063948] |
BMC Complementary and Alternative Medicine | Identification of plasma protein markers common to patients with malignant tumour and Abnormal Savda in Uighur medicine: a prospective clinical study [Pubmed:25652121] |
Atherosclerosis | Increased adipose tissue secretion of Fetuin-A, lipopolysaccharide-binding protein and high-mobility group box protein 1 in metabolic syndrome [PubMed: 25978344] |
Annals of Gastroenterology | Systemic levels of human β-defensin 1 are elevated in patients with cirrhosis [PubMed: 26751578] |
Food Chem. | Comparative proteomics of milk fat globule membrane in goat colostrum and mature milk [Pubmed:27173528] |
Liver International | High serum lipopolysaccharide binding protein is associated with increased mortality in patients with decompensated cirrhosis [pubmed:27712029] |
Journal of Functional Foods | Modulation of gut microbiota and gut-generated metabolites by bitter melon results in improvement in the metabolic status in high fat diet-induced obese rats [10.1016/j.jff.2017.12.050] |
Scientific Reports | Adamdec1, Ednrb and Ptgs1/Cox1, inflammation genes upregulated in the intestinal mucosa of obese rats, are downregulated by three probiotic strains. [pubmed:28512356] |
Journal of ethnopharmacology | A standardized extract of the fruit of Hovenia dulcis alleviated alcohol-induced hangover in healthy subjects with heterozygous ALDH2: A randomized, controlled, crossover trial. [pubmed:28750942] |
Journal of?Allergy?and Clinical Immunology | On a Western diet, APOE ?4 is associated with low innate immune sensing, but not APOE ?3 [Pubmed:29928926] |
Journal of?Animal?Sciences | Influence of yeast culture and feed antibiotics on ruminal fermentation and site and extent of digestion in beef heifers fed high grain rations [Pubmed:30060086] |
Journal?of?Animal?Science | Using ruminally protected and nonprotected active dried yeast as alternatives to antibiotics in finishing beef steers: growth performance, carcass traits, blood?… [Pubmed: 30184125] |
BMC?Complementary and Alternative?Medicine | Evaluation of the effect of Lactobacillus reuteri V3401 on biomarkers of inflammation, cardiovascular risk and liver steatosis in obese adults with metabolic?… [Pubmed: 30453950] |
Cell Communication and Signaling | Oridonin protects LPS-induced acute lung injury by modulating Nrf2-mediated oxidative stress and Nrf2-independent NLRP3 and NF-κB pathways [] |
Nutrients | High-dose Glycerol Monolaurate Up-Regulated Beneficial Indigenous Microbiota without Inducing Metabolic Dysfunction and Systemic Inflammation: New Insights?… [] |
Journal of Animal Science | Ruminally protected and unprotected Saccharomyces cerevisiae fermentation products as alternatives to antibiotics in finishing beef steers [Pubmed: 31410465] |
Nutrients | Lactobacillus reuteri V3401 Reduces Inflammatory Biomarkers and Modifies the Gastrointestinal Microbiome in Adults with Metabolic Syndrome: The PROSIR Study [Pubmed: 31370223] |
journal of veterinary pharmacology and therapeutics | Effects of sinomenine in LPS‐associated diseases are related to inhibition of LBP, Mac‐1, and L‐selectin levels [Pubmed: 31490576] |
Amyloid | Needle-shaped amyloid deposition in rat mammary gland: evidence of a novel amyloid fibril protein [Pubmed: 31615282] |
Food Research International | Ethanol Extract of Propolis Prevents High-Fat Diet-Induced Insulin Resistance and Obesity in Association with Modulation of Gut Microbiota in Mice [Pubmed: 32156386] |
Journal of Translational Medicine | Gut microbiota from coronary artery disease patients contributes to vascular dysfunction in mice by regulating bile acid metabolism and immune activation [Pubmed: 33036625] |
Communications Biology | Oxidation of fish oil exacerbates alcoholic liver disease by enhancing intestinal dysbiosis in mice [Pubmed: 32879433] |
MOLECULAR NUTRITION & FOOD RESEARCH | Antibiotic Treatment Reduces the Health Benefits of Soy Protein [Pubmed: 32729948] |
JOURNAL OF AGRICULTURAL AND FOOD CHEMISTRY | Highly Branched RG-I Domain Enrichment are Indispensable for Pectin Mitigating Against High-Fat Diet-Induced Obesity [Pubmed: 32633953] |
fish & shellfish immunology | Molecular characterization and expression analysis of chemokine (CXCL12) from Nile tilapia (Oreochromis niloticus) [Pubmed: 32540504] |
Scientific Reports | Tandem Mass Tag (TMT)-based quantitative proteomics reveals potential targets associated with onset of Sub-clinical Mastitis in cows [Pubmed: 32518370] |
experimental eye research | Corneal epithelial injury-induced norepinephrine promotes Pseudomonas aeruginosa keratitis [Pubmed: 32376471] |
Frontiers in Microbiology | Naringin Attenuates High Fat Diet Induced Non-alcoholic Fatty Liver Disease and Gut Bacterial Dysbiosis in Mice [] |
Journal of Burn Care & Research | Umbilical cord mesenchymal stem cells (hUCMSCs) for inflammatory regulation after excision and grafting of severe burn wounds in rats [33313794] |
JOURNAL OF ANIMAL SCIENCE | Processing Index of Barley Grain and Dietary Undigested Neutral Detergent Fiber Concentration Affected Chewing Behavior, Ruminal pH and Total Tract Nutrient??- [33474564] |
journal of agricultural and food chemistry | Influence of a Biotechnologically Produced Oyster Mushroom (Pleurotus sajor-caju) on the Gut Microbiota and Microbial Metabolites in Obese Zucker Rats [33497213] |
JOURNAL OF HAZARDOUS MATERIALS | Melatonin alleviates Ochratoxin A-induced liver inflammation involved intestinal microbiota homeostasis and intestinal microbiota-independent manner [33582472] |
Doctoral Thesis | Studies on Mastitis Caused by Translocated-Bacterial Components in Ruminants [] |
Arthritis Res Ther | Monocyte transcriptomes from patients with axial spondyloarthritis reveal dysregulated monocytopoiesis and a distinct inflammatory imprint [34560894] |
Microorganisms | Composition and Functions of the Gut Microbiome in Pediatric Obesity: Relationships with Markers of Insulin Resistance [34361925] |
Antioxidants | The Prebiotic Potential of Geraniin and Geraniin-Enriched Extract against High-Fat-Diet-Induced Metabolic Syndrome in Sprague Dawley Rats [Pubmed:35453317] |
Microbiome | Elucidating the role of the gut microbiota in the physiological effects of dietary fiber [Pubmed:35562794] |
Bioscience of Microbiota Food and Health | Local free fatty acids trigger the expression of lipopolysaccharide-binding protein in murine white adipose tissue [Pubmed:35433160] |
Siberian Medical Review | Сorrelation between the level of 25 (OH) D3 in blood, the content of antimicrobial peptides in oral fluid and dental caries intensity in young individuals [] |