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耐酒石酸酸性磷酸酶(ACP5)等多因子檢測試劑盒(流式熒光發(fā)光法)

Multiplex Assay Kit for Acid Phosphatase 5, Tartrate Resistant (ACP5) ,etc. by FLIA (Flow Luminescence Immunoassay)

TRAP; TrATPase; Tartrate Resistant Acid Phosphatase; Type 5 acid phosphatase

（注：單次混測多因子不超過8個指標(biāo) ）

  • 耐酒石酸酸性磷酸酶(ACP5)等多因子檢測試劑盒(流式熒光發(fā)光法) 產(chǎn)品包裝(模擬)
  • 耐酒石酸酸性磷酸酶(ACP5)等多因子檢測試劑盒(流式熒光發(fā)光法) 產(chǎn)品包裝(模擬)
  • Certificate 通過ISO 9001、ISO 13485質(zhì)量體系認(rèn)證

特異性

本試劑盒用于檢測耐酒石酸酸性磷酸酶(ACP5)等多因子檢測試劑盒(流式熒光發(fā)光法)，經(jīng)檢測與其它相似物質(zhì)無明顯交叉反應(yīng)。
由于受到技術(shù)及樣本來源的限制，不可能完成對所有相關(guān)或相似物質(zhì)交叉反應(yīng)檢測，因此本試劑盒有可能與未經(jīng)檢測的其它物質(zhì)有交叉反應(yīng)。

回收率

分別于定值血清及血漿樣本中加入一定量的耐酒石酸酸性磷酸酶(ACP5)等多因子檢測試劑盒(流式熒光發(fā)光法)（加標(biāo)樣品），重復(fù)測定并計算其均值，回收率為測定值與理論值的比率。

樣本 回收率范圍(%) 平均回收率(%)
serum(n=5) 84-101 88
EDTA plasma(n=5) 90-97 93
heparin plasma(n=5) 79-105 91

精密度

精密度用樣品測定值的變異系數(shù)CV表示。CV(%) = SD/mean×100
批內(nèi)差：取同批次試劑盒對低、中、高值定值樣本進(jìn)行定量檢測,每份樣本連續(xù)測定20 次，分別計算不同濃度樣本的平均值及SD值。
批間差：選取3個不同批次的試劑盒分別對低、中、高值定值樣本進(jìn)行定量測定，每個樣本使用同一試劑盒重復(fù)測定8次，分別計算不同濃度樣本的平均值及SD值。
批內(nèi)差： CV<10%
批間差： CV<12%

線性

在定值血清及血漿樣本內(nèi)加入適量的耐酒石酸酸性磷酸酶(ACP5)等多因子檢測試劑盒(流式熒光發(fā)光法)，并倍比稀釋成1:2，1:4，1:8，1:16的待測樣本，線性范圍即為稀釋后樣本中耐酒石酸酸性磷酸酶(ACP5)等多因子檢測試劑盒(流式熒光發(fā)光法)含量的測定值與理論值的比率。

樣本 1:2 1:4 1:8 1:16
serum(n=5) 91-98% 99-105% 79-102% 86-101%
EDTA plasma(n=5) 90-97% 97-105% 87-101% 86-95%
heparin plasma(n=5) 97-105% 88-101% 89-101% 85-101%

穩(wěn)定性

經(jīng)測定，試劑盒在有效期內(nèi)按推薦溫度保存，其活性降低率小于5%。
為減小外部因素對試劑盒破壞前后檢測值的影響，實驗室的環(huán)境條件需盡量保持一致，尤其是實驗室內(nèi)溫度、濕度及溫育條件。其次由同一實驗員來進(jìn)行操作可減少人為誤差。

實驗流程

1. 實驗前標(biāo)準(zhǔn)品、試劑及樣本準(zhǔn)備；
2. 加樣（標(biāo)準(zhǔn)品、樣本、磁珠）標(biāo)準(zhǔn)品或樣本100μL及磁珠10μL，
    37°C酶標(biāo)板振蕩器孵育90分鐘；
3. 磁吸甩干，加檢測溶液A100μL，37°C酶標(biāo)板振蕩器孵育60分鐘；
4. 磁吸洗板3次；
5. 加檢測溶液B100μL，37°C振動孵育30分鐘；
6. 磁吸洗板3次；
7. 加鞘液100μL，旋渦震蕩2分鐘后讀數(shù)。

實驗原理

將耐酒石酸酸性磷酸酶(ACP5)等多因子檢測試劑盒(流式熒光發(fā)光法)抗體包被于磁珠，制成固相載體，向微孔中分別加入標(biāo)準(zhǔn)品或標(biāo)本以及磁珠，其中的耐酒石酸酸性磷酸酶(ACP5)等多因子檢測試劑盒(流式熒光發(fā)光法)與連接于固相載體上的抗體結(jié)合，然后加入生物素化的耐酒石酸酸性磷酸酶(ACP5)等多因子檢測試劑盒(流式熒光發(fā)光法)抗體，將未結(jié)合的生物素化抗體洗凈后，加入PE標(biāo)記的親和素，再次徹底洗滌后即可上機讀數(shù)。MFI值和樣品中的耐酒石酸酸性磷酸酶(ACP5)等多因子檢測試劑盒(流式熒光發(fā)光法)呈正相關(guān)。

贈品

相關(guān)產(chǎn)品

編號 適用物種：Mus musculus (Mouse，小鼠) 應(yīng)用(僅供研究使用，不用于臨床診斷！)
RPA902Mu01 耐酒石酸酸性磷酸酶(ACP5)重組蛋白 Positive Control; Immunogen; SDS-PAGE; WB.
RPA902Mu02 耐酒石酸酸性磷酸酶(ACP5)重組蛋白 Positive Control; Immunogen; SDS-PAGE; WB.
PAA902Mu02 耐酒石酸酸性磷酸酶(ACP5)多克隆抗體 WB; IHC; ICC; IP.
PAA902Mu01 耐酒石酸酸性磷酸酶(ACP5)多克隆抗體 WB; IHC; ICC; IP.
SEA902Mu 耐酒石酸酸性磷酸酶(ACP5)檢測試劑盒(酶聯(lián)免疫吸附試驗法) Enzyme-linked immunosorbent assay for Antigen Detection.
MEA902Mu 耐酒石酸酸性磷酸酶(ACP5)檢測試劑盒(酶聯(lián)免疫吸附試驗法,小樣本) Enzyme-linked immunosorbent assay for Antigen Detection.
LMA902Mu 耐酒石酸酸性磷酸酶(ACP5)等多因子檢測試劑盒(流式熒光發(fā)光法) FLIA Kit for Antigen Detection.

參考文獻(xiàn)

雜志 參考文獻(xiàn)
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Evidence-Based Complementary and Alternative Medicine Antiosteoporosis Activity of New Oriental Medicine Preparation (Kyungokgo Mixed with Water Extract of Hovenia dulcis) on the Ovariectomized Mice [Pubmed:25737735]
Advances in Medical Sciences The influence of bexarotene, a selective agonist of the retinoid receptor X (RXR), and tazarotene, a selective agonist of the retinoid acid receptor (RAR), on bone metabolism in rats [PubMed: 26569440]
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Biomedicine & Pharmacotherapy Anti-osteoporotic effects of an antidepressant tianeptine on ovariectomized rats [S0753332216322752]
Clinical Trials in Degenerative Diseases Correlating oxidative stress-related factors with bone metabolic markers in older adult male patients exhibiting degenerative osteoporosis in the high-altitude hypoxic area of China: study protocol for a non-randomized controlled trial [issn:2542-3975]
Scientific Reports Evidence for excessive osteoclast activation in SIRT6 null mice [Pubmed:30030453]
International?Journal?of?Molecular?Sciences? Suppression Effect of Astaxanthin on Osteoclast Formation In Vitro and Bone Loss In Vivo [Pubmed:29562730]
Experimental Gerontology The decline of whole-body glucose metabolism in ovariectomized rats [Pubmed: 30292771]
Bone?Research NUMB maintains bone mass by promoting degradation of PTEN and GLI1 via ubiquitination in osteoblasts [Pubmed: 30455992]
Cell Death & Disease QKI deficiency leads to osteoporosis by promoting RANKL-induced osteoclastogenesis and disrupting bone metabolism [Pubmed: 32382069]
Effect of Corticopuncture (CP), Photobiomodulation (PBM) and the Combined Method on the Rate of Tooth Movement and Root Resorption: A Molecular, Histological … []
Evid Based Complement Alternat Med Gujiansan Ameliorates Avascular Necrosis of the Femoral Head by Regulating Autophagy via the HIF-1α/BNIP3 Pathway [34512780]
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