欧美综合自拍亚洲综合网_欧美黑人巨大videos异族_可乐操中文字幕_亚洲成av人福利专区网站_国产强奸视频在播放_免费无码国产在线53_最近最新的免費中文字幕_抖音免费视频素材_亚洲精品熟女一二区_国产无套粉嫩白浆在线麻豆

天冬氨酸轉(zhuǎn)氨酶(AST)等多因子檢測試劑盒(流式熒光發(fā)光法)

Multiplex Assay Kit for Aspartate Aminotransferase (AST) ,etc. by FLIA (Flow Luminescence Immunoassay)

cCAT; AAT; ASAT; SGOT; GOT1; Cysteine transaminase, cytoplasmic; Transaminase A; Aspartate Transaminase 1,Cytoplasmic; Glutamic-Oxaloacetic Transaminase 1,Soluble

（注：單次混測多因子不超過8個指標(biāo) ）

  • 天冬氨酸轉(zhuǎn)氨酶(AST)等多因子檢測試劑盒(流式熒光發(fā)光法) 產(chǎn)品包裝(模擬)
  • 天冬氨酸轉(zhuǎn)氨酶(AST)等多因子檢測試劑盒(流式熒光發(fā)光法) 產(chǎn)品包裝(模擬)
  • Certificate 通過ISO 9001、ISO 13485質(zhì)量體系認(rèn)證

特異性

本試劑盒用于檢測天冬氨酸轉(zhuǎn)氨酶(AST)等多因子檢測試劑盒(流式熒光發(fā)光法)，經(jīng)檢測與其它相似物質(zhì)無明顯交叉反應(yīng)。
由于受到技術(shù)及樣本來源的限制，不可能完成對所有相關(guān)或相似物質(zhì)交叉反應(yīng)檢測，因此本試劑盒有可能與未經(jīng)檢測的其它物質(zhì)有交叉反應(yīng)。

回收率

分別于定值血清及血漿樣本中加入一定量的天冬氨酸轉(zhuǎn)氨酶(AST)等多因子檢測試劑盒(流式熒光發(fā)光法)（加標(biāo)樣品），重復(fù)測定并計(jì)算其均值，回收率為測定值與理論值的比率。

樣本 回收率范圍(%) 平均回收率(%)
serum(n=5) 97-104 101
EDTA plasma(n=5) 79-105 89
heparin plasma(n=5) 85-94 89

精密度

精密度用樣品測定值的變異系數(shù)CV表示。CV(%) = SD/mean×100
批內(nèi)差：取同批次試劑盒對低、中、高值定值樣本進(jìn)行定量檢測,每份樣本連續(xù)測定20 次，分別計(jì)算不同濃度樣本的平均值及SD值。
批間差：選取3個不同批次的試劑盒分別對低、中、高值定值樣本進(jìn)行定量測定，每個樣本使用同一試劑盒重復(fù)測定8次，分別計(jì)算不同濃度樣本的平均值及SD值。
批內(nèi)差： CV<10%
批間差： CV<12%

線性

在定值血清及血漿樣本內(nèi)加入適量的天冬氨酸轉(zhuǎn)氨酶(AST)等多因子檢測試劑盒(流式熒光發(fā)光法)，并倍比稀釋成1:2，1:4，1:8，1:16的待測樣本，線性范圍即為稀釋后樣本中天冬氨酸轉(zhuǎn)氨酶(AST)等多因子檢測試劑盒(流式熒光發(fā)光法)含量的測定值與理論值的比率。

樣本 1:2 1:4 1:8 1:16
serum(n=5) 81-101% 81-98% 87-96% 78-92%
EDTA plasma(n=5) 80-101% 81-90% 98-105% 90-101%
heparin plasma(n=5) 79-101% 86-101% 79-98% 95-104%

穩(wěn)定性

經(jīng)測定，試劑盒在有效期內(nèi)按推薦溫度保存，其活性降低率小于5%。
為減小外部因素對試劑盒破壞前后檢測值的影響，實(shí)驗(yàn)室的環(huán)境條件需盡量保持一致，尤其是實(shí)驗(yàn)室內(nèi)溫度、濕度及溫育條件。其次由同一實(shí)驗(yàn)員來進(jìn)行操作可減少人為誤差。

實(shí)驗(yàn)流程

1. 實(shí)驗(yàn)前標(biāo)準(zhǔn)品、試劑及樣本準(zhǔn)備；
2. 加樣（標(biāo)準(zhǔn)品、樣本、磁珠）標(biāo)準(zhǔn)品或樣本100μL及磁珠10μL，
    37°C酶標(biāo)板振蕩器孵育90分鐘；
3. 磁吸甩干，加檢測溶液A100μL，37°C酶標(biāo)板振蕩器孵育60分鐘；
4. 磁吸洗板3次；
5. 加檢測溶液B100μL，37°C振動孵育30分鐘；
6. 磁吸洗板3次；
7. 加鞘液100μL，旋渦震蕩2分鐘后讀數(shù)。

實(shí)驗(yàn)原理

將天冬氨酸轉(zhuǎn)氨酶(AST)等多因子檢測試劑盒(流式熒光發(fā)光法)抗體包被于磁珠，制成固相載體，向微孔中分別加入標(biāo)準(zhǔn)品或標(biāo)本以及磁珠，其中的天冬氨酸轉(zhuǎn)氨酶(AST)等多因子檢測試劑盒(流式熒光發(fā)光法)與連接于固相載體上的抗體結(jié)合，然后加入生物素化的天冬氨酸轉(zhuǎn)氨酶(AST)等多因子檢測試劑盒(流式熒光發(fā)光法)抗體，將未結(jié)合的生物素化抗體洗凈后，加入PE標(biāo)記的親和素，再次徹底洗滌后即可上機(jī)讀數(shù)。MFI值和樣品中的天冬氨酸轉(zhuǎn)氨酶(AST)等多因子檢測試劑盒(流式熒光發(fā)光法)呈正相關(guān)。

贈品

相關(guān)產(chǎn)品

編號 適用物種：Mus musculus (Mouse，小鼠) 應(yīng)用(僅供研究使用，不用于臨床診斷！)
RPB214Mu01 天冬氨酸轉(zhuǎn)氨酶(AST)重組蛋白 Positive Control; Immunogen; SDS-PAGE; WB.
RPB214Mu02 天冬氨酸轉(zhuǎn)氨酶(AST)重組蛋白 Positive Control; Immunogen; SDS-PAGE; WB.
APB214Mu01 天冬氨酸轉(zhuǎn)氨酶(AST)活性蛋白 Cell?culture;?Activity?Assays.
PAB214Mu01 天冬氨酸轉(zhuǎn)氨酶(AST)多克隆抗體 WB; IHC; ICC; IP.
PAB214Mu02 天冬氨酸轉(zhuǎn)氨酶(AST)多克隆抗體 WB; IHC; ICC; IP.
LAB214Mu81 天冬氨酸轉(zhuǎn)氨酶(AST)多克隆抗體(異硫氰酸熒光素標(biāo)記) WB; IHC; ICC; IF.
LAB214Mu71 天冬氨酸轉(zhuǎn)氨酶(AST)多克隆抗體(生物素標(biāo)記) WB; IHC; ICC.
MAB214Mu22 天冬氨酸轉(zhuǎn)氨酶(AST)單克隆抗體 WB; IHC; ICC; IP.
MAB214Mu21 天冬氨酸轉(zhuǎn)氨酶(AST)單克隆抗體 WB; IHC; ICC; IP.
MEB214Mu 天冬氨酸轉(zhuǎn)氨酶(AST)檢測試劑盒(酶聯(lián)免疫吸附試驗(yàn)法,小樣本) Enzyme-linked immunosorbent assay for Antigen Detection.
IEB214Mu 天冬氨酸轉(zhuǎn)氨酶(AST)檢測試劑盒(酶聯(lián)免疫吸附試驗(yàn)法,快捷型) Enzyme-linked immunosorbent assay for Antigen Detection.
SEB214Mu 天冬氨酸轉(zhuǎn)氨酶(AST)檢測試劑盒(酶聯(lián)免疫吸附試驗(yàn)法) Enzyme-linked immunosorbent assay for Antigen Detection.
LMB214Mu 天冬氨酸轉(zhuǎn)氨酶(AST)等多因子檢測試劑盒(流式熒光發(fā)光法) FLIA Kit for Antigen Detection.
PSB214Mu01 天冬氨酸轉(zhuǎn)氨酶(AST)抗體對 ELISA; CLIA; ELISPOT; Luminex; Immunochromatography and other Immunoassays.
KSB214Mu01 天冬氨酸轉(zhuǎn)氨酶(AST)檢測試劑盒DIY材料(酶聯(lián)免疫吸附試驗(yàn)法) Main materials for "Do It (ELISA Kit) Yourself".

參考文獻(xiàn)

雜志 參考文獻(xiàn)
Kidney International Adrenocorticotropic hormone ameliorates acute kidney injury by steroidogenic-dependent and -independent mechanisms [PubMed: PMC3612362]
Proceedings of the National Academy of Sciences Pathogenesis of emerging severe fever with thrombocytopenia syndrome virus in C57/BL6 mouse model [PubMed: PMC3382536]
African Journal of Pharmacy and Pharmacology Protective effect of Panax ginseng against N-acetyl-p-aminophenol-induced hepatotoxicity in rats [Academicjournals: Source]
Kidney International Adrenocorticotropic hormone ameliorates acute kidney injury by steroidogenic-dependent and-independent mechanisms [Pubmed: 23325074]
European Journal of Pharmacology Protective effect of Et-1 receptor antagonist bosentan on paracetamol induced acute liver toxicity in rats [ScienceDirect: S0014299914000235]
Journal of Immunology The P2X1 Receptor Is Required for Neutrophil Extravasation during Lipopolysaccharide-Induced Lethal Endotoxemia in Mice [Pubmed:25480563]
Gastroenterol Res Pract Assessing the Effect of Leptin on Liver Damage in Case of Hepatic Injury Associated with Paracetamol Poisoning [PubMed: 26697061]
Gastroenterol Res Pract. Advanced Studies in Clinical and Experimental Research in Gastroenterology [Pubmed:26955389]
Scientific Reports Adiponectin?protects?the?rats?liver?against?chronic?intermittent?hypoxia?induced?injury?throughAMP-activated?protein?kinase?pathway. [pubmed:27678302]
?Biochimica et Biophysica Acta (BBA)-General Subjects Modulation of gut microbiota contributes to curcumin-mediated attenuation of hepatic steatosis in rats [pubmed:28341485]
Scientific Reports Deletion of MCP-1 Impedes Pathogenesis of Acid Ceramidase Deficiency [Pubmed:29379059]
FEBS?Open?Bio A critical role of hepatitis B virus polymerase in cirrhosis, hepatocellular carcinoma, and steatosis [Pubmed:29321963]
BMC?Complementary and Alternative?Medicine? Hepatoprotective activity of Erythrina× neillii leaf extract and characterization of its phytoconstituents [Pubmed: 28095910]
laboratory investigation Hepatic pathology and altered gene transcription in a murine model of acid ceramidase deficiency [Pubmed: 31186526]
Brain Behavior and Immunity Corticosterone-mediated microglia activation affects dendritic spine plasticity and motor learning functions in minimal hepatic encephalopathy [Pubmed: 31437533]
Biomed Research International Liraglutide Attenuates Nonalcoholic Fatty Liver Disease by Modulating Gut Microbiota in Rats Administered a High-Fat Diet [Pubmed: 32149099]
Nanomaterials Biological Safety and Biodistribution of Chitosan Nanoparticles [Pubmed: 32340313]
European Journal of Biology Effects of Tempol in Lipopolysaccharide-Induced Liver Injury []
Pathogens Antimalarial Effect of the Total Glycosides of the Medicinal Plant, Ranunculus japonicus [33925018]
Int Immunopharmacol Taraxasterol mitigates Con A-induced hepatitis in mice by suppressing interleukin-2 expression and its signaling in T lymphocytes [34848154]
Acta Pharmacol Sin NFAT inhibitor 11R-VIVIT ameliorates mouse renal fibrosis after ischemia-reperfusion-induced acute kidney injury [34937917]
J Magn Reson Imaging Water Specific MRI T1 Mapping for Evaluating Liver Inflammation Activity Grades in Rats With Methionine‐Choline‐Deficient Diet‐Induced Nonalcoholic Fatty Liver?… [Pubmed:35212074]
Medcomm Blockade of mIL‐6R alleviated lipopolysaccharide‐induced systemic inflammatory response syndrome by suppressing NF‐κB‐mediated Ccl2 expression and?… [Pubmed:35548710]
International Immunopharmacology Dimethyl fumarate ameliorates autoimmune hepatitis in mice by blocking NLRP3 inflammasome activation [Pubmed:35605433]
留言咨詢