胱天蛋白酶9(CASP9)等多因子檢測試劑盒(流式熒光發(fā)光法)
Multiplex Assay Kit for Caspase 9 (CASP9) ,etc. by FLIA (Flow Luminescence Immunoassay)
APAF3; ICE-LAP6; MCH6; Apoptosis-Related Cysteine Peptidase; Cysteinyl Aspartate Specific Proteinases 9; Apoptotic protease-activating factor 3; ICE-like apoptotic protease 6
(注:單次混測多因子不超過8個指標(biāo) )
- 編號LMA627Hu
- 物種Homo sapiens (Human,人) 相同的名稱,不同的物種。
- 實驗方法雙抗夾心
- 反應(yīng)時長3.5h
- 檢測范圍0.02-20ng/mL
- 靈敏度最小可檢測劑量小于等于0.007 ng/mL.
- 樣本類型Tissue homogenates, cell lysates, cell culture supernates and other biological fluids.
- 下載 英文說明書 中文說明書
- 規(guī)格 8指標(biāo)數(shù) 7指標(biāo)數(shù) 6指標(biāo)數(shù) 5指標(biāo)數(shù) 4指標(biāo)數(shù) 3指標(biāo)數(shù) 2指標(biāo)數(shù)1指標(biāo)數(shù)
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特異性
本試劑盒用于檢測胱天蛋白酶9(CASP9)等多因子檢測試劑盒(流式熒光發(fā)光法),經(jīng)檢測與其它相似物質(zhì)無明顯交叉反應(yīng)。
由于受到技術(shù)及樣本來源的限制,不可能完成對所有相關(guān)或相似物質(zhì)交叉反應(yīng)檢測,因此本試劑盒有可能與未經(jīng)檢測的其它物質(zhì)有交叉反應(yīng)。
回收率
分別于定值血清及血漿樣本中加入一定量的胱天蛋白酶9(CASP9)等多因子檢測試劑盒(流式熒光發(fā)光法)(加標(biāo)樣品),重復(fù)測定并計算其均值,回收率為測定值與理論值的比率。
樣本 | 回收率范圍(%) | 平均回收率(%) |
serum(n=5) | 78-97 | 81 |
EDTA plasma(n=5) | 91-99 | 94 |
heparin plasma(n=5) | 86-101 | 89 |
sodium citrate plasma(n=5) | 85-97 | 90 |
精密度
精密度用樣品測定值的變異系數(shù)CV表示。CV(%) = SD/mean×100
批內(nèi)差:取同批次試劑盒對低、中、高值定值樣本進行定量檢測,每份樣本連續(xù)測定20 次,分別計算不同濃度樣本的平均值及SD值。
批間差:選取3個不同批次的試劑盒分別對低、中、高值定值樣本進行定量測定,每個樣本使用同一試劑盒重復(fù)測定8次,分別計算不同濃度樣本的平均值及SD值。
批內(nèi)差: CV<10%
批間差: CV<12%
線性
在定值血清及血漿樣本內(nèi)加入適量的胱天蛋白酶9(CASP9)等多因子檢測試劑盒(流式熒光發(fā)光法),并倍比稀釋成1:2,1:4,1:8,1:16的待測樣本,線性范圍即為稀釋后樣本中胱天蛋白酶9(CASP9)等多因子檢測試劑盒(流式熒光發(fā)光法)含量的測定值與理論值的比率。
樣本 | 1:2 | 1:4 | 1:8 | 1:16 |
serum(n=5) | 80-95% | 98-105% | 88-99% | 95-104% |
EDTA plasma(n=5) | 98-105% | 79-101% | 79-104% | 88-104% |
heparin plasma(n=5) | 78-94% | 85-97% | 78-99% | 90-103% |
sodium citrate plasma(n=5) | 99-105% | 92-101% | 94-101% | 95-103% |
穩(wěn)定性
經(jīng)測定,試劑盒在有效期內(nèi)按推薦溫度保存,其活性降低率小于5%。
為減小外部因素對試劑盒破壞前后檢測值的影響,實驗室的環(huán)境條件需盡量保持一致,尤其是實驗室內(nèi)溫度、濕度及溫育條件。其次由同一實驗員來進行操作可減少人為誤差。
實驗流程
1. 實驗前標(biāo)準品、試劑及樣本準備;
2. 加樣(標(biāo)準品、樣本、磁珠)標(biāo)準品或樣本100μL及磁珠10μL,
37°C酶標(biāo)板振蕩器孵育90分鐘;
3. 磁吸甩干,加檢測溶液A100μL,37°C酶標(biāo)板振蕩器孵育60分鐘;
4. 磁吸洗板3次;
5. 加檢測溶液B100μL,37°C振動孵育30分鐘;
6. 磁吸洗板3次;
7. 加鞘液100μL,旋渦震蕩2分鐘后讀數(shù)。
實驗原理
將胱天蛋白酶9(CASP9)等多因子檢測試劑盒(流式熒光發(fā)光法)抗體包被于磁珠,制成固相載體,向微孔中分別加入標(biāo)準品或標(biāo)本以及磁珠,其中的胱天蛋白酶9(CASP9)等多因子檢測試劑盒(流式熒光發(fā)光法)與連接于固相載體上的抗體結(jié)合,然后加入生物素化的胱天蛋白酶9(CASP9)等多因子檢測試劑盒(流式熒光發(fā)光法)抗體,將未結(jié)合的生物素化抗體洗凈后,加入PE標(biāo)記的親和素,再次徹底洗滌后即可上機讀數(shù)。MFI值和樣品中的胱天蛋白酶9(CASP9)等多因子檢測試劑盒(流式熒光發(fā)光法)呈正相關(guān)。
贈品
增值服務(wù)
相關(guān)產(chǎn)品
編號 | 適用物種:Homo sapiens (Human,人) | 應(yīng)用(僅供研究使用,不用于臨床診斷!) |
RPA627Hu04 | 胱天蛋白酶9(CASP9)重組蛋白 | Positive Control; Immunogen; SDS-PAGE; WB. |
RPA627Hu03 | 胱天蛋白酶9(CASP9)重組蛋白 | Positive Control; Immunogen; SDS-PAGE; WB. |
RPA627Hu01 | 胱天蛋白酶9(CASP9)重組蛋白 | Positive Control; Immunogen; SDS-PAGE; WB. |
RPA627Hu02 | 胱天蛋白酶9(CASP9)重組蛋白 | Positive Control; Immunogen; SDS-PAGE; WB. |
PAA627Hu02 | 胱天蛋白酶9(CASP9)多克隆抗體 | WB; IHC; ICC; IP. |
PAA627Hu03 | 胱天蛋白酶9(CASP9)多克隆抗體 | WB; IHC; ICC; IP. |
PAA627Hu01 | 胱天蛋白酶9(CASP9)多克隆抗體 | WB; FCM |
LAA627Hu81 | 胱天蛋白酶9(CASP9)多克隆抗體(異硫氰酸熒光素標(biāo)記) | WB; IHC; ICC; IF. |
LAA627Hu71 | 胱天蛋白酶9(CASP9)多克隆抗體(生物素標(biāo)記) | WB; IHC; ICC. |
MAA627Hu23 | 胱天蛋白酶9(CASP9)單克隆抗體 | WB; IHC; ICC; IP. |
MAA627Hu27 | 胱天蛋白酶9(CASP9)單克隆抗體 | WB; IHC; ICC; IP. |
MAA627Hu29 | 胱天蛋白酶9(CASP9)單克隆抗體 | WB; IHC; ICC; IP. |
MAA627Hu21 | 胱天蛋白酶9(CASP9)單克隆抗體 | WB; IHC; ICC; IP. |
MAA627Hu24 | 胱天蛋白酶9(CASP9)單克隆抗體 | WB; IHC; ICC; IP. |
MAA627Hu25 | 胱天蛋白酶9(CASP9)單克隆抗體 | WB; IHC; ICC; IP. |
MAA627Hu22 | 胱天蛋白酶9(CASP9)單克隆抗體 | WB,IHC |
MEA627Hu | 胱天蛋白酶9(CASP9)檢測試劑盒(酶聯(lián)免疫吸附試驗法,小樣本) | Enzyme-linked immunosorbent assay for Antigen Detection. |
SEA627Hu | 胱天蛋白酶9(CASP9)檢測試劑盒(酶聯(lián)免疫吸附試驗法) | Enzyme-linked immunosorbent assay for Antigen Detection. |
LMA627Hu | 胱天蛋白酶9(CASP9)等多因子檢測試劑盒(流式熒光發(fā)光法) | FLIA Kit for Antigen Detection. |
KSA627Hu01 | 胱天蛋白酶9(CASP9)檢測試劑盒DIY材料(酶聯(lián)免疫吸附試驗法) | Main materials for "Do It (ELISA Kit) Yourself". |
參考文獻
雜志 | 參考文獻 |
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Molecular Neurobiology | Ursodeoxycholic Acid Ameliorates Apoptotic Cascade in the Rotenone Model of Parkinson’s Disease: Modulation of Mitochondrial Perturbations [Pubmed:25502462] |
BMC Cancer | Saikosaponin?d?induces?cell?death?through?caspase-3-dependent,?caspase-3-independent?andmitochondrial?pathways?in?mammalian?hepatic?stellate?cells. [pubmed:27461108] |
Exp Lung Res | Stretch-induced apoptosis in rat alveolar epithelial cells is mediated by the intrinsic mitochondrial pathway. [pubmed:28394655] |
The American Journal of Chinese Medicine | Saikosaponin a Induces Apoptosis through Mitochondria-Dependent Pathway in Hepatic Stellate Cells [pubmed:28231747] |
Frontiers in Pharmacology | Docking Studies and Biological Evaluation of a Potential β-Secretase Inhibitor of 3-Hydroxyhericenone F from Hericium erinaceus. [pubmed:28553224] |
Clinical?And Experimental Pharmacology And Physiology? | Diallyl sulfide alleviates cisplatin‐induced nephrotoxicity in rats via suppressing NF‐κB downstream inflammatory proteins and p53/Puma signalling pathway [Pubmed:29266336] |
Environmental Toxicology | Antihepatocarcinogenic activity of whey protein concentrate and lactoferrin in diethylnitrosamine‐treated male albino mice [Pubmed: 31087429] |
European journal of medicinal chemistry | Synthesis of novel S-linked dihydroartemisinin derivatives and evaluation of their anticancer activity [Pubmed: 31216504] |
Odontology | Effects of different pulp-capping materials on cell death signaling pathways of lipoteichoic acid-stimulated human dental pulp stem cells [Pubmed: 33206337] |
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SAGE Journals | Insights Into Protective Mechanisms of Dandelion Leaf Extract Against Cisplatin-Induced Nephrotoxicity in Rats: Role of Inhibitory Effect on Inflammatory and Apoptotic?… [] |
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