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胰高血糖素(GCG)等多因子檢測試劑盒(流式熒光發(fā)光法)

Multiplex Assay Kit for Glucagon (GCG) ,etc. by FLIA (Flow Luminescence Immunoassay)

GC; GLP1; GLP2; GRPP; Glicentin-Related Polypeptide; Glucagen; Oxyntomodulin; Incretin hormone

（注：單次混測多因子不超過8個指標 ）

  • 胰高血糖素(GCG)等多因子檢測試劑盒(流式熒光發(fā)光法) 產品包裝(模擬)
  • 胰高血糖素(GCG)等多因子檢測試劑盒(流式熒光發(fā)光法) 產品包裝(模擬)
  • Certificate 通過ISO 9001、ISO 13485質量體系認證

特異性

本試劑盒用于檢測胰高血糖素(GCG)等多因子檢測試劑盒(流式熒光發(fā)光法)，經檢測與其它相似物質無明顯交叉反應。
由于受到技術及樣本來源的限制，不可能完成對所有相關或相似物質交叉反應檢測，因此本試劑盒有可能與未經檢測的其它物質有交叉反應。

回收率

分別于定值血清及血漿樣本中加入一定量的胰高血糖素(GCG)等多因子檢測試劑盒(流式熒光發(fā)光法)（加標樣品），重復測定并計算其均值，回收率為測定值與理論值的比率。

樣本 回收率范圍(%) 平均回收率(%)
serum(n=5) 94-102 97
EDTA plasma(n=5) 99-105 102
heparin plasma(n=5) 87-95 90

精密度

精密度用樣品測定值的變異系數CV表示。CV(%) = SD/mean×100
批內差：取同批次試劑盒對低、中、高值定值樣本進行定量檢測,每份樣本連續(xù)測定20 次，分別計算不同濃度樣本的平均值及SD值。
批間差：選取3個不同批次的試劑盒分別對低、中、高值定值樣本進行定量測定，每個樣本使用同一試劑盒重復測定8次，分別計算不同濃度樣本的平均值及SD值。
批內差： CV<10%
批間差： CV<12%

線性

在定值血清及血漿樣本內加入適量的胰高血糖素(GCG)等多因子檢測試劑盒(流式熒光發(fā)光法)，并倍比稀釋成1:2，1:4，1:8，1:16的待測樣本，線性范圍即為稀釋后樣本中胰高血糖素(GCG)等多因子檢測試劑盒(流式熒光發(fā)光法)含量的測定值與理論值的比率。

樣本 1:2 1:4 1:8 1:16
serum(n=5) 93-103% 88-104% 83-101% 92-104%
EDTA plasma(n=5) 81-92% 90-99% 99-105% 91-104%
heparin plasma(n=5) 99-105% 93-101% 84-93% 80-98%

穩(wěn)定性

經測定，試劑盒在有效期內按推薦溫度保存，其活性降低率小于5%。
為減小外部因素對試劑盒破壞前后檢測值的影響，實驗室的環(huán)境條件需盡量保持一致，尤其是實驗室內溫度、濕度及溫育條件。其次由同一實驗員來進行操作可減少人為誤差。

實驗流程

1. 實驗前標準品、試劑及樣本準備；
2. 加樣（標準品、樣本、磁珠、檢測溶液A）標準品或樣本50μL及磁珠10μL，加檢測溶液A50μL，
    37°C酶標板振蕩器孵育60分鐘；
3. 磁吸洗板3次；
4. 加檢測溶液B100μL，37°C振動孵育30分鐘；
5. 磁吸洗板3次；
6. 加鞘液100μL，旋渦震蕩2分鐘后讀數。

實驗原理

將胰高血糖素(GCG)等多因子檢測試劑盒(流式熒光發(fā)光法)抗體包被于磁珠，制成固相載體，向微孔中分別加入標準品或標本以及磁珠、標記VEGFA，標記的胰高血糖素(GCG)等多因子檢測試劑盒(流式熒光發(fā)光法)和未標記的胰高血糖素(GCG)等多因子檢測試劑盒(流式熒光發(fā)光法)與連接于固相載體上的抗體競爭結合，然后將未結合物洗凈后，加入PE標記的親和素，再次徹底洗滌后即可上機讀數。MFI值和樣品中的胰高血糖素(GCG)等多因子檢測試劑盒(流式熒光發(fā)光法)呈負相關。

贈品

相關產品

編號 適用物種：Chicken (Gallus，雞) 應用(僅供研究使用，不用于臨床診斷！)
RPB266Ga01 胰高血糖素(GCG)重組蛋白 Positive Control; Immunogen; SDS-PAGE; WB.
PAB266Ga01 胰高血糖素(GCG)多克隆抗體 WB; IHC; ICC; IP.
MAB266Ga21 胰高血糖素(GCG)單克隆抗體 WB; IHC; ICC; IP.
CEB266Ga 胰高血糖素(GCG)檢測試劑盒(酶聯(lián)免疫吸附試驗法) Enzyme-linked immunosorbent assay for Antigen Detection.
LMB266Ga 胰高血糖素(GCG)等多因子檢測試劑盒(流式熒光發(fā)光法) FLIA Kit for Antigen Detection.

參考文獻

雜志 參考文獻
Molecular Metabolism PER2 promotes glucose storage to liver glycogen during feeding and acute fasting by inducing Gys2 PTG and GL expression [PubMed: PMC3773838]
Exp Biol Med (Maywood). Increasing glucagon secretion could antagonize the action of exogenous insulin for glycemic control in streptozocin-induced diabetic rhesus monkeys. [Pubmed: 23760004]
European Journal of Endocrinology Specificity and sensitivity of commercially available assays for glucagon and oxyntomodulin measurement in human [Eje-online: Source]
American Diabetes Association IL-21 is a major negative regulator of IRF4-dependent lipolysis affecting Tregs in adipose tissue and systemic insulin sensitivity [Pubmed:24430438]
Journal of Pharmacy and Pharmacology Enzogenol improves diabetes-related metabolic change in C57BL/KsJ-db/db?mice, a model of type 2 diabetes mellitus [Pubmed:24611864]
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Appl Physiol Nutr Metab The glucoreCavia (Guinea pig )latory response to high-intensity aerobic exercise following training in rats with insulin-treated type 1 diabetes mellitus [Pubmed:27175938]
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