Gremlin 1蛋白(GREM1)等多因子檢測試劑盒(流式熒光發(fā)光法)
Multiplex Assay Kit for Gremlin 1 (GREM1) ,etc. by FLIA (Flow Luminescence Immunoassay)
PIG2; CKTSF1B1; DAND2; IHG-2; DAND2; DRM; Increased in high glucose protein 2; Cell proliferation-inducing gene 2 protein; Cysteine knot superfamily 1, BMP antagonist 1
(注:單次混測多因子不超過8個指標 )
- 編號LMC128Mu
- 物種Mus musculus (Mouse,小鼠) 相同的名稱,不同的物種。
- 實驗方法雙抗夾心
- 反應時長3.5h
- 檢測范圍0.01-10ng/mL
- 靈敏度最小可檢測劑量小于等于0.003 ng/mL.
- 樣本類型Serum, plasma and other biological fluids
- 下載 英文說明書 中文說明書
- 規(guī)格 8指標數(shù) 7指標數(shù) 6指標數(shù) 5指標數(shù) 4指標數(shù) 3指標數(shù) 2指標數(shù)1指標數(shù)
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特異性
本試劑盒用于檢測Gremlin 1蛋白(GREM1)等多因子檢測試劑盒(流式熒光發(fā)光法),經檢測與其它相似物質無明顯交叉反應。
由于受到技術及樣本來源的限制,不可能完成對所有相關或相似物質交叉反應檢測,因此本試劑盒有可能與未經檢測的其它物質有交叉反應。
回收率
分別于定值血清及血漿樣本中加入一定量的Gremlin 1蛋白(GREM1)等多因子檢測試劑盒(流式熒光發(fā)光法)(加標樣品),重復測定并計算其均值,回收率為測定值與理論值的比率。
樣本 | 回收率范圍(%) | 平均回收率(%) |
serum(n=5) | 93-101 | 97 |
EDTA plasma(n=5) | 84-101 | 94 |
heparin plasma(n=5) | 83-94 | 90 |
sodium citrate plasma(n=5) | 81-94 | 88 |
精密度
精密度用樣品測定值的變異系數(shù)CV表示。CV(%) = SD/mean×100
批內差:取同批次試劑盒對低、中、高值定值樣本進行定量檢測,每份樣本連續(xù)測定20 次,分別計算不同濃度樣本的平均值及SD值。
批間差:選取3個不同批次的試劑盒分別對低、中、高值定值樣本進行定量測定,每個樣本使用同一試劑盒重復測定8次,分別計算不同濃度樣本的平均值及SD值。
批內差: CV<10%
批間差: CV<12%
線性
在定值血清及血漿樣本內加入適量的Gremlin 1蛋白(GREM1)等多因子檢測試劑盒(流式熒光發(fā)光法),并倍比稀釋成1:2,1:4,1:8,1:16的待測樣本,線性范圍即為稀釋后樣本中Gremlin 1蛋白(GREM1)等多因子檢測試劑盒(流式熒光發(fā)光法)含量的測定值與理論值的比率。
樣本 | 1:2 | 1:4 | 1:8 | 1:16 |
serum(n=5) | 81-93% | 97-105% | 82-101% | 84-99% |
EDTA plasma(n=5) | 97-105% | 97-104% | 83-101% | 89-101% |
heparin plasma(n=5) | 91-98% | 95-103% | 80-93% | 79-101% |
sodium citrate plasma(n=5) | 87-102% | 86-95% | 81-102% | 78-101% |
穩(wěn)定性
經測定,試劑盒在有效期內按推薦溫度保存,其活性降低率小于5%。
為減小外部因素對試劑盒破壞前后檢測值的影響,實驗室的環(huán)境條件需盡量保持一致,尤其是實驗室內溫度、濕度及溫育條件。其次由同一實驗員來進行操作可減少人為誤差。
實驗流程
1. 實驗前標準品、試劑及樣本準備;
2. 加樣(標準品、樣本、磁珠)標準品或樣本100μL及磁珠10μL,
37°C酶標板振蕩器孵育90分鐘;
3. 磁吸甩干,加檢測溶液A100μL,37°C酶標板振蕩器孵育60分鐘;
4. 磁吸洗板3次;
5. 加檢測溶液B100μL,37°C振動孵育30分鐘;
6. 磁吸洗板3次;
7. 加鞘液100μL,旋渦震蕩2分鐘后讀數(shù)。
實驗原理
將Gremlin 1蛋白(GREM1)等多因子檢測試劑盒(流式熒光發(fā)光法)抗體包被于磁珠,制成固相載體,向微孔中分別加入標準品或標本以及磁珠,其中的Gremlin 1蛋白(GREM1)等多因子檢測試劑盒(流式熒光發(fā)光法)與連接于固相載體上的抗體結合,然后加入生物素化的Gremlin 1蛋白(GREM1)等多因子檢測試劑盒(流式熒光發(fā)光法)抗體,將未結合的生物素化抗體洗凈后,加入PE標記的親和素,再次徹底洗滌后即可上機讀數(shù)。MFI值和樣品中的Gremlin 1蛋白(GREM1)等多因子檢測試劑盒(流式熒光發(fā)光法)呈正相關。
贈品
增值服務
相關產品
編號 | 適用物種:Mus musculus (Mouse,小鼠) | 應用(僅供研究使用,不用于臨床診斷!) |
RPC128Mu01 | Gremlin 1蛋白(GREM1)重組蛋白 | Positive Control; Immunogen; SDS-PAGE; WB. |
RPC128Mu02 | Gremlin 1蛋白(GREM1)重組蛋白 | Positive Control; Immunogen; SDS-PAGE; WB. |
APC128Mu01 | Gremlin 1蛋白(GREM1)活性蛋白 | Cell?culture;?Activity?Assays. |
PAC128Mu01 | Gremlin 1蛋白(GREM1)多克隆抗體 | WB; IHC |
PAC128Mu02 | Gremlin 1蛋白(GREM1)多克隆抗體 | WB; IHC; ICC; IP. |
LAC128Mu81 | Gremlin 1蛋白(GREM1)多克隆抗體(異硫氰酸熒光素標記) | WB; IHC; ICC; IF. |
LAC128Mu71 | Gremlin 1蛋白(GREM1)多克隆抗體(生物素標記) | WB; IHC; ICC. |
SEC128Mu | Gremlin 1蛋白(GREM1)檢測試劑盒(酶聯(lián)免疫吸附試驗法) | Enzyme-linked immunosorbent assay for Antigen Detection. |
LMC128Mu | Gremlin 1蛋白(GREM1)等多因子檢測試劑盒(流式熒光發(fā)光法) | FLIA Kit for Antigen Detection. |
參考文獻
雜志 | 參考文獻 |
Lung | Intrinsic BMP Antagonist Gremlin-1 as a Novel Circulating Marker in Pulmonary Arterial Hypertension [PubMed: 25926293] |
Tumour Biol | Overexpression of Gremlin promotes non-small cell lung cancer progression [PubMed: 26392110] |
Int J Cardiol | Triple head-to-head comparison of fibrotic biomarkers galectin-3, osteopontin and gremlin-1 for long-term prognosis in suspected and proven acute heart failure patients [PubMed: 26539964] |
International Journal of Cardiology | Triple?head-to-head?comparison?of?fibrotic?biomarkers?galectin-3,?osteopontin?and?gremlin-1?for?long-termprognosis?in?suspected?and?proven?acute?heart?failure?patients [Pubmed:26539964] |
American Journal of Respiratory Cell and Molecular Biology | BMPR2 Mutation-independent Mechanisms of Disrupted BMP Signaling in IPAH [Pubmed:27187737] |
J Rheumatol | GREM1 Is a Key ReCavia (Guinea pig )lator of Synoviocyte Hyperplasia and Invasiveness [Pubmed:26834210] |
European cells & materials | The BMP2 variant L51P restores the osteogenic differentiation of human mesenchymal stromal cells in the presence of intervertebral disc cells. [pubmed:28266688] |
Sci Rep. | Genomic reprograming analysis of the Mesothelial to Mesenchymal Transition identifies biomarkers in peritoneal dialysis patients. [pubmed:28327551] |
American Journal of Respiratory Cell and Molecular Biology | Bone Morphogenic Protein Type 2 Receptor Mutation-Independent Mechanisms of Disrupted Bone Morphogenetic Protein Signaling in Idiopathic Pulmonary Arterial … [rcmb.2015-0402OC] |
Molecular Medicine Reports | Gremlin-1 suppression increases BMP-2-induced osteogenesis of human mesenchymal stem cells [pubmed:28260028] |
Cellular Haemostasis and Platelets | Platelets as a novel source of Gremlin-1: Implications for thrombo inflammation [pubmed:27929199] |
Scientific Reports | Gremlin, A Potential Urinary Biomarker of Anca-Associated Crescentic Glomerulonephritis [Pubmed: 31053735] |
International Journal of Molecular Sciences | Transcriptomic and Proteomic Analyses Reveal the Potential Mode of Action of Chondrocyte Sheets in Hyaline Cartilage Regeneration [Pubmed: 31878307] |
American Journal of Physiology-Cell Physiology | Suppression of microRNA-23a/b-3p delays the development degenerative joint disease by activating Grem1 and TGF-β/Smad signaling pathway [Pubmed: 32186934] |
Inflammopharmacology | Protective role of microRNA-23a/b-3p inhibition against osteoarthritis through Gremlin1-depenent activation of TGF-β/smad signaling in chondrocytesa [Pubmed:35441352] |
Mol Carcinog | CAF‐derived exosomes transmitted Gremlin‐1 promotes cancer progression and decreases the sensitivity of hepatoma cells to sorafenib [Pubmed:35638711] |