欧美综合自拍亚洲综合网_欧美黑人巨大videos异族_可乐操中文字幕_亚洲成av人福利专区网站_国产强奸视频在播放_免费无码国产在线53_最近最新的免費中文字幕_抖音免费视频素材_亚洲精品熟女一二区_国产无套粉嫩白浆在线麻豆

鐵調(diào)素(Hepc)等多因子檢測試劑盒(流式熒光發(fā)光法)

Multiplex Assay Kit for Hepcidin (Hepc) ,etc. by FLIA (Flow Luminescence Immunoassay)

HAMP; HFE2B; PLTR; LEAP1; Hepcidin Antimicrobial Peptide; Liver-expressed antimicrobial peptide 1; Putative liver tumor regressor

（注：單次混測多因子不超過8個指標(biāo) ）

  • 鐵調(diào)素(Hepc)等多因子檢測試劑盒(流式熒光發(fā)光法) 產(chǎn)品包裝(模擬)
  • 鐵調(diào)素(Hepc)等多因子檢測試劑盒(流式熒光發(fā)光法) 產(chǎn)品包裝(模擬)
  • Certificate 通過ISO 9001、ISO 13485質(zhì)量體系認證

特異性

本試劑盒用于檢測鐵調(diào)素(Hepc)等多因子檢測試劑盒(流式熒光發(fā)光法)，經(jīng)檢測與其它相似物質(zhì)無明顯交叉反應(yīng)。
由于受到技術(shù)及樣本來源的限制，不可能完成對所有相關(guān)或相似物質(zhì)交叉反應(yīng)檢測，因此本試劑盒有可能與未經(jīng)檢測的其它物質(zhì)有交叉反應(yīng)。

回收率

分別于定值血清及血漿樣本中加入一定量的鐵調(diào)素(Hepc)等多因子檢測試劑盒(流式熒光發(fā)光法)（加標(biāo)樣品），重復(fù)測定并計算其均值，回收率為測定值與理論值的比率。

樣本 回收率范圍(%) 平均回收率(%)
serum(n=5) 97-105 102
EDTA plasma(n=5) 95-102 99
heparin plasma(n=5) 96-104 101

精密度

精密度用樣品測定值的變異系數(shù)CV表示。CV(%) = SD/mean×100
批內(nèi)差：取同批次試劑盒對低、中、高值定值樣本進行定量檢測,每份樣本連續(xù)測定20 次，分別計算不同濃度樣本的平均值及SD值。
批間差：選取3個不同批次的試劑盒分別對低、中、高值定值樣本進行定量測定，每個樣本使用同一試劑盒重復(fù)測定8次，分別計算不同濃度樣本的平均值及SD值。
批內(nèi)差： CV<10%
批間差： CV<12%

線性

在定值血清及血漿樣本內(nèi)加入適量的鐵調(diào)素(Hepc)等多因子檢測試劑盒(流式熒光發(fā)光法)，并倍比稀釋成1:2，1:4，1:8，1:16的待測樣本，線性范圍即為稀釋后樣本中鐵調(diào)素(Hepc)等多因子檢測試劑盒(流式熒光發(fā)光法)含量的測定值與理論值的比率。

樣本 1:2 1:4 1:8 1:16
serum(n=5) 83-93% 84-98% 78-101% 86-98%
EDTA plasma(n=5) 90-98% 93-105% 96-105% 84-91%
heparin plasma(n=5) 78-93% 88-101% 78-95% 90-104%

穩(wěn)定性

經(jīng)測定，試劑盒在有效期內(nèi)按推薦溫度保存，其活性降低率小于5%。
為減小外部因素對試劑盒破壞前后檢測值的影響，實驗室的環(huán)境條件需盡量保持一致，尤其是實驗室內(nèi)溫度、濕度及溫育條件。其次由同一實驗員來進行操作可減少人為誤差。

實驗流程

1. 實驗前標(biāo)準品、試劑及樣本準備；
2. 加樣（標(biāo)準品、樣本、磁珠）標(biāo)準品或樣本100μL及磁珠10μL，
    37°C酶標(biāo)板振蕩器孵育90分鐘；
3. 磁吸甩干，加檢測溶液A100μL，37°C酶標(biāo)板振蕩器孵育60分鐘；
4. 磁吸洗板3次；
5. 加檢測溶液B100μL，37°C振動孵育30分鐘；
6. 磁吸洗板3次；
7. 加鞘液100μL，旋渦震蕩2分鐘后讀數(shù)。

實驗原理

將鐵調(diào)素(Hepc)等多因子檢測試劑盒(流式熒光發(fā)光法)抗體包被于磁珠，制成固相載體，向微孔中分別加入標(biāo)準品或標(biāo)本以及磁珠，其中的鐵調(diào)素(Hepc)等多因子檢測試劑盒(流式熒光發(fā)光法)與連接于固相載體上的抗體結(jié)合，然后加入生物素化的鐵調(diào)素(Hepc)等多因子檢測試劑盒(流式熒光發(fā)光法)抗體，將未結(jié)合的生物素化抗體洗凈后，加入PE標(biāo)記的親和素，再次徹底洗滌后即可上機讀數(shù)。MFI值和樣品中的鐵調(diào)素(Hepc)等多因子檢測試劑盒(流式熒光發(fā)光法)呈正相關(guān)。

贈品

相關(guān)產(chǎn)品

編號 適用物種：Canis familiaris; Canine (Dog，犬) 應(yīng)用(僅供研究使用，不用于臨床診斷！)
RPB979Ca01 鐵調(diào)素(Hepc)重組蛋白 Positive Control; Immunogen; SDS-PAGE; WB.
SEB979Ca 鐵調(diào)素(Hepc)檢測試劑盒(酶聯(lián)免疫吸附試驗法) Enzyme-linked immunosorbent assay for Antigen Detection.
LMB979Ca 鐵調(diào)素(Hepc)等多因子檢測試劑盒(流式熒光發(fā)光法) FLIA Kit for Antigen Detection.

參考文獻

雜志 參考文獻
World J Gastroenterol. Hepcidin expression in colon during trinitrobenzene sulfonic acid-induced colitis in rats [Pubmed: 24764672]
International Journal of Advanced Research Modulations of serum hepcidin, IL6 and iron status by different exercise regimens in ovarectomized rats [Com:Source]
Neurochemical Journal Serum iron metabolism markers including hepcidin in multiple sclerosis patients [Springer:Source]
Afro-Egypt J Infect Endem Dis Monitoring Hepcidin Level in Chronic Hepatitis C Virus Patients during Therapy [Zu:Source]
Gastroenterology Gluconeogenic signals regulate iron homeostasis via hepcidin in mice [Pubmed:Pmc3989026]
J Card Fail. Inappropriate expression of hepcidin by liver congestion contributes to anemia and relative iron deficiency [Pubmed:24440572]
Food Funct. The action of JAK, SMAD and ERK signal pathways on hepcidin suppression by polysaccharides from Angelica sinensis in rats with iron deficiency anemia [Pubmed:24752529]
Free Radic Res. Changes in skeletal muscle iron metabolism outpace amyotrophic lateral sclerosis onset in transgenic rats bearing the G93A hmSOD1 gene mutation [Pubmed:25175826]
Saudi J Kidney Dis Transpl. Hepcidin and regulation of iron homeostasis in maintenance hemodialysis patients [Pubmed:25193892]
J Endocrinol Invest.? The interplay among iron metabolism, endothelium and inflammatory cascade in dysmetabolic disorders [Pubmed:25245337]
Gastroenterology Research and Practice An Evaluation of the Correlation between Hepcidin Serum Levels and Disease Activity in Inflammatory Bowel Disease [Pubmed:25628652]
Clin Lab. Serum hepcidin levels in Bulgarian population. [Pubmed:25651734]
The Tohoku Journal of Experimental Medicine Liver Congestion in Heart Failure Contributes to Inappropriately Increased Serum Hepcidin despite Anemia [Pubmed:25742771]
Cancer Res Hepcidin regulation in prostate and its disruption in prostate cancer [PubMed: 25858146]
Biochem Biophys Res Commun B-cell translocation gene 2 promotes hepatic hepcidin production via induction of Yin Yang 1 [PubMed: 25839654]
Blood Purif Effect of a More Permeable Dialysis Membrane on ESA Resistance in Hemodialysis Patients - A Pilot Investigation [PubMed: 26536083]
Asian Journal of Clinical Nutrition Urinary Hepcidin in Diagnosis of Iron Deficiency Anemia [Openview: E01Ad7C4A9109Ed5Fadb03Ec206D7Bdc]
Front Psychol Ferrous sulfate, but not iron polymaltose complex, aggravates local and systemic inflammation and oxidative stress in dextran sodium sulfate-induced colitis in rats [PubMed: 25852597]
Biol Trace Elem Res Iron Supplementation Attenuates the Inflammatory Status of Anemic Piglets by Regulating Hepcidin [PubMed: 25774043]
APS Journals Kupffer cell depletion protects against the steatosis, but not the liver damage, induced by marginal copper, high fructose diet in male rats. [PubMed: 25813056]
European Journal of Haematology Hepcidin in tumor‐related iron deficiency anemia and tumor‐related anemia of chronic disease: pathogenic mechanisms and diagnosis [PubMed: 24954786]
WJA The Effects of Treatment on Serum Hepcidin and Iron Homeostasis in HIV-1-Infected In-dividuals [Journal: 59107]
Nutrition, Metabolism and Cardiovascular Diseases Hepcidin?and?iron?metabolism?associated?with?cardiometabolic?risk factors?in?children: A case-control?study. [Pubmed:27139516]
Food Funct The effects of polysaccharides from the root of Angelica sinensis on tumor growth and iron metabolism in H22-bearing mice [Pubmed:26757699]
Antioxid Redox Signal. Hydrogen sulfide attenuates inflammatory hepcidin by reducing IL-6 secretion and promoting SIRT1-mediated STAT3 deacetylation [Pubmed:26154696]
BioMed Research International The Impact of Acute Matriptase Inhibition in Hepatic Inflammatory Models. [pubmed:27642598]
Frontiers in Neuroscience In Absence of the Cellular Prion Protein, Alterations in Copper Metabolism and Copper-Dependent Oxidase Activity Affect Iron Distribution. [pubmed:27729845]
Experimental Biology and Medicine Effects of iron supplementation in mice with hypoferremia induced by obesity [pubmed:27439539]
Nutrition, Metabolism and Cardiovascular Diseases Hepcidin and iron metabolism associated with cardiometabolic risk factors in children: A case–control study [pubmed:27139516]
PLOS?ONE S-Propargyl-Cysteine, a?Novel?Hydrogen?Sulfide?Donor,?Inhibits?Inflammatory?Hepcidin?and Relieves Anemia of Inflammation by Inhibiting IL-6/STAT3 Pathway. [pubmed:27649298]
Endocrine connections Hepcidin links gluco-toxicity to pancreatic beta cell dysfunction by inhibiting Pdx-1 expression [pubmed:28179377]
Maternal and cord blood hepcidin levels based on gestational weeks in term and preterm infants [publication:305921433_Maternal_and_cord_blood_hepc]
Oxidative Medicine and Cellular Longevity Acidic Polysaccharide from Angelica sinensis Reverses Anemia of Chronic Disease Involving the Suppression of Inflammatory Hepcidin and NF-κB Activation [pubmed:29147463]
The Royal Society of Chemistry Silkworm feces extract improves iron deficiency anemia via suppressing hepcidin expression and promoting iron-regulatory proteins expression [DOI: 10.1039/C7RA09576G]
Journal of Inflammation Evaluation of a competitive hepcidin ELISA assay in the differential diagnosis of iron deficiency anaemia with concurrent inflammation and anaemia of inflammation in elderly patients [10.1186:s12950-017-0166-3]
Antioxidants and Redox Signaling AMPK serves as a Therapeutic Target against Anemia of Inflammation [pubmed:27923278]
NutriciónHospitalaria Micronutrient supplementation in gastric bypass surgery: prospective study on in??? ammation and iron metabolism in premenopausal women [10.20960/nh.1162]
The Japanese Society of Hematology Hepcidin suppression in β?thalassemia is associated with the down?regulation of atonal homolog 8 [pubmed:28405918]
Tuberculosis Modulation of iron status biomarkers in tuberculosis-diabetes co-morbidity [10.1016/j.tube.2017.11.011]
Oman Medical Journal The Role of Insulin Therapy in Correcting Hepcidin Levels in Patients with Type 2 Diabetes Mellitus. [pubmed:28584599]
Cellular?Physiology and Biochemistry The Effects of Angelica Sinensis Polysaccharide on Tumor Growth and Iron Metabolism by Regulating Hepcidin in Tumor-Bearing Mice [Pubmed:29843136]
Cellular?Microbiology? inhibits ferroportin translation by modulating FBXL5‐IRP2 axis for its growth within host macrophages [Pubmed:29470856]
Journal of Histochemistry & Cytochemistry Acute and Chronic Iron Overloading Differentially Modulates the Expression of Cellular Iron-homeostatic Molecules in Normal Rat Kidney [Pubmed:29873589]
Clinical and Experimental?Medicine Increased NGAL level associated with iron store in chronic kidney disease with anemia [Pubmed:29909502]
Hematology Relationship between obesity and iron deficiency anemia: is there a role of hepcidin? [Pubmed:29318945]
Hepcidin serum levels in HCV chronically mono-infected na?ve patients, compared to healthy individuals [77856]
Food & Function Effect of iron supplement from microalgae on the treatment of iron-deficient anemia in rats [Doi: 10.1039/C8FO01696H]
Haematologica New thiazolidinones reduce iron overload in mouse models of hereditary hemochromatosis and β-thalassemia [Pubmed: 30792208]
Ann Intensive Care Inflammatory anemia-associated parameters are related to 28-day mortality in patients with sepsis admitted to the ICU: a preliminary observational study [Pubmed: 31183575]
Journal of Functional Foods Dietary gelatin enhances non-heme iron absorption possibly via regulation of systemic iron homeostasis in rats []
Naunyn Schmiedebergs Arch Pharmacol. 3-Amidinophenylalanine-derived matriptase inhibitors can modulate hepcidin production in vitro [Pubmed: 31659405]
DEVELOPMENT GROWTH & DIFFERENTIATION H2O2 induces oxidative stress damage through the BMP‐6/SMAD/hepcidin axis [Pubmed: 32012242]
Frontiers in Physiology Increased Hepcidin Levels During a Period of High Training Load Do Not Alter Iron Status in Male Elite Junior Rowers [Pubmed: 32038278]
J Korean Med Sci Can Calprotectin Show Subclinical Inflammation in Familial Mediterranean Fever Patients? [Pubmed: 32174062]
nephrology dialysis transplantation Iron kinetics following treatment with sucroferric oxyhydroxide or ferric citrate in healthy rats and models of anaemia, iron overload or inflammation [Pubmed: 32259248]
mikrochimica acta Gold-silver core-shell nanoparticle–based impedimetric immunosensor for detection of iron homeostasis biomarker hepcidin [Pubmed: 33095336]
JOURNAL OF STEROID BIOCHEMISTRY AND MOLECULAR BIOLOGY Circulating Osteocyte‐Related Biomarkers (vitamin D, sclerostin, dickkopf-1), hepcidin, and oxidative stress markers in early breast cancer: their impact in?… [Pubmed: 33065276]
Sleep Breath Relation of serum hepcidin levels and restless legs syndrome in chronic hemodialysis patients [Pubmed: 33029690]
journal of trace elements in experimental medicine Difference in iron metabolism may partly explain sex-related variability in the manifestation of Wilson's disease [Pubmed: 32937238]
reproduction in domestic animals Expression of iron regulatory proteins in full term swine placenta [Pubmed: 32449967]
Neurobiology of Aging Estrogen deficiency is associated with brain iron deposition via upregulation of hepcidin expression in aged female mice [Pubmed: 32920472]
The Egyptian Journal of Haematology Urinary hepcidin concentration in assessment of iron homeostasis in pediatrics []
COMPARATIVE MONITORING OF THE ENZYME ACTIVITY OF BONE METABOLISM IN PATIENTS AFTER DENTAL IMPLANTATION []
Serum iron, prealbumin, D-dimer and prothrombin time are indicators of the diagnosis, severity and outcome of Salmonella enterica serovar Enteritidis infection []
Гепсидин и индекс фиброза печени MELD-XI как маркеры полиорганной недостаточности у пациентов хронической сердечной недостаточностью с?… []
Ассоциации уровней NT-proBNP и гепсидина с клинико-лабораторными параметрами у больных хронической сердечной недостаточностью с разной … []
Biosci Rep Correlation of hepcidin and serum ferritin levels in thalassemia patients at Chiang Mai University Hospital [33565577]
Biometals Iron metabolism is disturbed and anti-copper treatment improves but does not normalize iron metabolism in Wilson's disease [33555495]
J Mol Cell Cardiol Systemic iron deficiency does not affect the cardiac iron content and progression of heart failure [34139233]
Continuous Veno-Venous Hemofiltration (CVVH) Improves Iron Metabolism Disorders in Patients With Sepsis: A Single-Center Prospective Cohort Study []
Reports of Biochemistry & Molecular Biology Evaluation of Serum Cyclooxygenase, Hepcidin Levels in Acute Renal Injury (AKI) Patients Following Cardiac Catheterization []
Food Funct High-dose ferric citrate supplementation attenuates omega-3 polyunsaturated fatty acid biosynthesis via downregulating delta 5 and 6 desaturases in rats with high-fat?… [34787162]
J Trace Elem Med Biol Caffeine and alcohol-friends or foes of human iron stores? [Pubmed:35063815]
PeerJ Resveratrol ameliorates iron overload induced liver fibrosis in mice by regulating iron homeostasis [Pubmed:35698613]
Serum Calprotectin and Iron Metabolism Biomarker Levels in Beh?et's Disease []
Food Bioscience Roles of homopolymeric apoferritin in alleviating alcohol-induced liver injury []
留言咨詢