欧美综合自拍亚洲综合网_欧美黑人巨大videos异族_可乐操中文字幕_亚洲成av人福利专区网站_国产强奸视频在播放_免费无码国产在线53_最近最新的免費中文字幕_抖音免费视频素材_亚洲精品熟女一二区_国产无套粉嫩白浆在线麻豆

免疫球蛋白E(IgE)等多因子檢測試劑盒(流式熒光發(fā)光法)

Multiplex Assay Kit for Immunoglobulin E (IgE) ,etc. by FLIA (Flow Luminescence Immunoassay)

IGHE; Immunoglobulin Heavy Constant Epsilon; Ig epsilon chain C region

（注：單次混測多因子不超過8個指標(biāo) ）

  • 免疫球蛋白E(IgE)等多因子檢測試劑盒(流式熒光發(fā)光法) 產(chǎn)品包裝(模擬)
  • 免疫球蛋白E(IgE)等多因子檢測試劑盒(流式熒光發(fā)光法) 產(chǎn)品包裝(模擬)
  • Certificate 通過ISO 9001、ISO 13485質(zhì)量體系認(rèn)證

特異性

本試劑盒用于檢測免疫球蛋白E(IgE)等多因子檢測試劑盒(流式熒光發(fā)光法)，經(jīng)檢測與其它相似物質(zhì)無明顯交叉反應(yīng)。
由于受到技術(shù)及樣本來源的限制，不可能完成對所有相關(guān)或相似物質(zhì)交叉反應(yīng)檢測，因此本試劑盒有可能與未經(jīng)檢測的其它物質(zhì)有交叉反應(yīng)。

回收率

分別于定值血清及血漿樣本中加入一定量的免疫球蛋白E(IgE)等多因子檢測試劑盒(流式熒光發(fā)光法)（加標(biāo)樣品），重復(fù)測定并計算其均值，回收率為測定值與理論值的比率。

樣本 回收率范圍(%) 平均回收率(%)
serum(n=5) 90-105 98
EDTA plasma(n=5) 80-98 87
heparin plasma(n=5) 86-95 91
sodium citrate plasma(n=5) 97-105 101

精密度

精密度用樣品測定值的變異系數(shù)CV表示。CV(%) = SD/mean×100
批內(nèi)差：取同批次試劑盒對低、中、高值定值樣本進行定量檢測,每份樣本連續(xù)測定20 次，分別計算不同濃度樣本的平均值及SD值。
批間差：選取3個不同批次的試劑盒分別對低、中、高值定值樣本進行定量測定，每個樣本使用同一試劑盒重復(fù)測定8次，分別計算不同濃度樣本的平均值及SD值。
批內(nèi)差： CV<10%
批間差： CV<12%

線性

在定值血清及血漿樣本內(nèi)加入適量的免疫球蛋白E(IgE)等多因子檢測試劑盒(流式熒光發(fā)光法)，并倍比稀釋成1:2，1:4，1:8，1:16的待測樣本，線性范圍即為稀釋后樣本中免疫球蛋白E(IgE)等多因子檢測試劑盒(流式熒光發(fā)光法)含量的測定值與理論值的比率。

樣本 1:2 1:4 1:8 1:16
serum(n=5) 83-104% 91-102% 86-97% 79-96%
EDTA plasma(n=5) 92-105% 90-98% 98-105% 98-105%
heparin plasma(n=5) 81-93% 93-101% 97-105% 79-89%
sodium citrate plasma(n=5) 83-102% 97-105% 78-98% 78-89%

穩(wěn)定性

經(jīng)測定，試劑盒在有效期內(nèi)按推薦溫度保存，其活性降低率小于5%。
為減小外部因素對試劑盒破壞前后檢測值的影響，實驗室的環(huán)境條件需盡量保持一致，尤其是實驗室內(nèi)溫度、濕度及溫育條件。其次由同一實驗員來進行操作可減少人為誤差。

實驗流程

1. 實驗前標(biāo)準(zhǔn)品、試劑及樣本準(zhǔn)備；
2. 加樣（標(biāo)準(zhǔn)品、樣本、磁珠）標(biāo)準(zhǔn)品或樣本100μL及磁珠10μL，
    37°C酶標(biāo)板振蕩器孵育90分鐘；
3. 磁吸甩干，加檢測溶液A100μL，37°C酶標(biāo)板振蕩器孵育60分鐘；
4. 磁吸洗板3次；
5. 加檢測溶液B100μL，37°C振動孵育30分鐘；
6. 磁吸洗板3次；
7. 加鞘液100μL，旋渦震蕩2分鐘后讀數(shù)。

實驗原理

將免疫球蛋白E(IgE)等多因子檢測試劑盒(流式熒光發(fā)光法)抗體包被于磁珠，制成固相載體，向微孔中分別加入標(biāo)準(zhǔn)品或標(biāo)本以及磁珠，其中的免疫球蛋白E(IgE)等多因子檢測試劑盒(流式熒光發(fā)光法)與連接于固相載體上的抗體結(jié)合，然后加入生物素化的免疫球蛋白E(IgE)等多因子檢測試劑盒(流式熒光發(fā)光法)抗體，將未結(jié)合的生物素化抗體洗凈后，加入PE標(biāo)記的親和素，再次徹底洗滌后即可上機讀數(shù)。MFI值和樣品中的免疫球蛋白E(IgE)等多因子檢測試劑盒(流式熒光發(fā)光法)呈正相關(guān)。

贈品

相關(guān)產(chǎn)品

編號 適用物種：Sus scrofa; Porcine (Pig，豬) 應(yīng)用(僅供研究使用，不用于臨床診斷！)
NPA545Po01 免疫球蛋白E(IgE)天然蛋白 Positive Control; Immunogen; SDS-PAGE; WB.
RPA545Po01 免疫球蛋白E(IgE)重組蛋白 Positive Control; Immunogen; SDS-PAGE; WB.
CPA545Po21 免疫球蛋白E(IgE)卵白蛋白偶聯(lián)物 Immunogen; SDS-PAGE; WB.
PAA545Po01 免疫球蛋白E(IgE)多克隆抗體 WB; IHC; ICC; IP.
PAA545Po08 免疫球蛋白E(IgE)多克隆抗體 WB; IHC; ICC; IP.
MAA545Po22 免疫球蛋白E(IgE)單克隆抗體 WB; IHC; ICC; IP.
MAA545Po21 免疫球蛋白E(IgE)單克隆抗體 WB; IHC; ICC; IP.
SEA545Po 免疫球蛋白E(IgE)檢測試劑盒(酶聯(lián)免疫吸附試驗法) Enzyme-linked immunosorbent assay for Antigen Detection.
SCA545Po 免疫球蛋白E(IgE)檢測試劑盒(化學(xué)發(fā)光免疫分析法) Chemiluminescent immunoassay for Antigen Detection.
LMA545Po 免疫球蛋白E(IgE)等多因子檢測試劑盒(流式熒光發(fā)光法) FLIA Kit for Antigen Detection.

參考文獻

雜志 參考文獻
Molecular Carcinogenesis IL17A gene polymorphisms, serum IL-17A and IgE levels, and hepatocellular carcinoma risk in patients with chronic hepatitis B virus infection [PubMed: 23280722]
Human Immunology IL21 and IL21R polymorphisms and their interactive effects on serum IL-21 and IgE levels in patients with chronic hepatitis B virus infection [PubMed: 23354321]
Scand J Immunol Passive transfer of tumour-derived MDSCs inhibits asthma-related airway inflammation [Pubmed: 24313384]
AAPS PharmSciTech Topical Lyogel Containing Corticosteroid Decreases IgE Expression and Enhances the Therapeutic Efficacy Against Atopic Eczema [PubMed: 25511806]
Ann Allergy Asthma Immunol Reduced airway microbiota diversity is associated with elevated allergic respiratory inflammation [PubMed: 26123423]
Molecular Medicine Reports The?protective?role?of?vitamin?D3?in a?murine?model?of?asthma?via?the?suppression?of TGF-β/Smad?signaling?and?activation?of the?Nrf2/HO-1?pathway. [pubmed:27484042]
Gut Pathog. Oral administration of Clostridium butyricum CGMCC0313‐1 reduces ovalbumin‐induced allergic airway inflammation in mice [pubmed:28250847]
International Immunopharmacology Catalpol alleviates ovalbumin-induced asthma in mice: Reduced eosinophil infiltration in the lung [pubmed:27992791]
Asian Pacific Society of Respirology Oral administration of Clostridium butyricum CGMCC0313-1 reduces ovalbumin-induced allergic airway inflammation in mice [pubmed:28122397]
Molecular Medicine Reports Protective effects of astragaloside IV against ovalbumin-induced allergic rhinitis are mediated by T-box protein expressed in T cells/GATA-3 and forkhead box protein 3/retinoic acid-related orphan nuclear receptor γt. [pubmed:28586019]
Journal of Autoimmunity Commensal bacteria aggravate allergic asthma via NLRP3/IL-1β signaling in post-weaning mice [10.1016:j.jaut.2018.07.003]
Water?Research? Sub-chronic inhalation of reclaimed water-induced fibrotic lesion in a mouse model [Pubmed:29655095]
Human & Experimental Toxicology Investigation of hypersensitivity potential of diacetyl by determining cytokine profiles [Pubmed:29233007]
Journal of?International?Medical?Research Changes in Th1/Th2-producing cytokines during acute exacerbation chronic obstructive pulmonary disease [Pubmed:29950127]
Journal Of?Cellular?Biochemistry Effects of catalpol on bronchial asthma and its relationship with cytokines [Pubmed: 30536454]
INTERNATIONAL JOURNAL OF MOLECULAR?SCIENCES? Adipose Tissue-Derived Mesenchymal Stem Cell Modulates the Immune Response of Allergic Rhinitis in a Rat Model [Pubmed: 30781605]
Royal?Society?Open?Science Metabolomics analysis of baicalin on ovalbumin-sensitized allergic rhinitis rats []
Health and Medicine Antiallergical Effect of New Combined Nazal Aerodisperse System in the Conditions of Experimental Allergic Rhinitis []
American Journal of Translational Research Inhibitory effects of catalpol coordinated with budesonide and their relationship with cytokines and Interleukin-13 expression [Pubmed: 31737193]
MOLECULAR IMMUNOLOGY Animal models for analysis of hypersensitivity reactions to Shuanghuanglian injection [Pubmed: 32302806]
bioscience reports Protective effect of Asarum sieboldii essential oil on ovalbumin induced allergic rhinitis in rat [Pubmed: 32395767]
international journal of antimicrobial agents Synergistic Phage-surfactant Combination Clears IgE-promoted Staphylococcus aureus Aggregation in Vitro and Enhances the Effect in Vivo [Pubmed: 32335278]
Aggravation of Airway Inflammation in RSV-Infected Asthmatic Mice Following Infection-Induced Alteration of Gut Microbiota []
ACS Biomaterials Science & Engineering Tissue-Engineered Decellularized Allografts for Anterior Cruciate Ligament Reconstruction []
Biomarkers of Allergic Asthma and their Association with Serum Parameters []
Biomed Pharmacother Qingfei oral liquid inhibited autophagy to alleviate inflammation via mTOR signaling pathway in RSV-infected asthmatic mice [33706133]
Environ Pollut Trace endotoxin in reclaimed water is only one of the risk sources in subchronic inhalation exposure [34090073]
Annals of Tropical Medicine & Public Health Effectiveness of biomarkers and serum parameters in determination allergic asthma and detection of its severity []
Mediators Inflamm Roles Played by the PI3K/Akt/HIF-1α Pathway and IL-17A in the Chinese Subtype of Chronic Sinusitis with Nasal Polyps [Pubmed:35075349]
Expert Opinion on Therapeutic Targets Dapagliflozin mitigates ovalbumin-prompted airway inflammatory-oxidative successions and associated bronchospasm in a rat model of allergic asthma [Pubmed:35549595]
留言咨詢